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The map label for this gene is folE [H]

Identifier: 146339979

GI number: 146339979

Start: 3185666

End: 3186373

Strand: Direct

Name: folE [H]

Synonym: BRADO2984

Alternate gene names: 146339979

Gene position: 3185666-3186373 (Clockwise)

Preceding gene: 146339977

Following gene: 146339980

Centisome position: 42.72

GC content: 67.09

Gene sequence:

>708_bases
ATGGACACTTTGATCAAGACGCTGCGCGCCAACAAGCCCGAGGGAAAGCCGTCTGAGACCAAGCTGGCCGGCGAACGACC
GGCGGAGCTCGATCCGGCCGAGTTCCTGGCTGCGGCCGTGCGCGCCGACCGCCCCCGGCCGTCGCGCGCCGAGGCGGAAG
CCGCGGTTTATACGCTGCTCAATTACATCGGTGAGGATCCGACGCGCGAGGGCCTGATCGATACGCCGCGCCGGGTGGTC
GAGGCCTATGACGAGCTGTTCCAGGGCTACCATCAGTGCCCGGCCGAGGTGCTCGACCGCACCTTCGGTGAGACCGCCGG
GTACGACGATTTCGTGCTGATTCGCGACATCGAGTTCACGTCGCAATGCGAGCATCACATGATGCCCTTCTACGGCCGCG
CGCACATCGCCTATACGCCGGTCGAGCGTGTCGTCGGGCTGTCCAAGCTGGCGCGCCTGACCGACATTTTCGCCCGCCGC
CTGCAGACCCAGGAGCATCTGACGGCGCAGATCGCGGCCGCGATCGACGAGGTGCTGAAGCCGCGCGGCGTCGCGGTGAT
GATCGAGGCGGAGCACACCTGCATGTCGGTGCGCGGCGTCGCCAAGCACGGCGCGGTCACCTTCACCAGTCGCTTCACCG
GCATGTTCCGCGACAATCCGGCGGAGCAGTCCCGCTTCCTCTCCATGGTGCGCTCGCCGCAACGCTGA

Upstream 100 bases:

>100_bases
GCCGAGCCTGCGGATCACACCTCTGCAGGTGACGCCGTCCAAGGTGACGCTCTGCCGGTGACACTCCGGTCGGTCCGCCG
ACCGGTCGAACGGAGACGAG

Downstream 100 bases:

>100_bases
GCGCAGCATATTTCGGCTGTTACAGGCCTCGATCAGAGAAACTCCGTGTCTTCATCCTCAGCTGAGCGCGAGGAGGGATT
GGCCTTCCTCCCGAAATTCG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MDTLIKTLRANKPEGKPSETKLAGERPAELDPAEFLAAAVRADRPRPSRAEAEAAVYTLLNYIGEDPTREGLIDTPRRVV
EAYDELFQGYHQCPAEVLDRTFGETAGYDDFVLIRDIEFTSQCEHHMMPFYGRAHIAYTPVERVVGLSKLARLTDIFARR
LQTQEHLTAQIAAAIDEVLKPRGVAVMIEAEHTCMSVRGVAKHGAVTFTSRFTGMFRDNPAEQSRFLSMVRSPQR

Sequences:

>Translated_235_residues
MDTLIKTLRANKPEGKPSETKLAGERPAELDPAEFLAAAVRADRPRPSRAEAEAAVYTLLNYIGEDPTREGLIDTPRRVV
EAYDELFQGYHQCPAEVLDRTFGETAGYDDFVLIRDIEFTSQCEHHMMPFYGRAHIAYTPVERVVGLSKLARLTDIFARR
LQTQEHLTAQIAAAIDEVLKPRGVAVMIEAEHTCMSVRGVAKHGAVTFTSRFTGMFRDNPAEQSRFLSMVRSPQR
>Mature_235_residues
MDTLIKTLRANKPEGKPSETKLAGERPAELDPAEFLAAAVRADRPRPSRAEAEAAVYTLLNYIGEDPTREGLIDTPRRVV
EAYDELFQGYHQCPAEVLDRTFGETAGYDDFVLIRDIEFTSQCEHHMMPFYGRAHIAYTPVERVVGLSKLARLTDIFARR
LQTQEHLTAQIAAAIDEVLKPRGVAVMIEAEHTCMSVRGVAKHGAVTFTSRFTGMFRDNPAEQSRFLSMVRSPQR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=219, Percent_Identity=42.9223744292237, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI4503949, Length=219, Percent_Identity=42.9223744292237, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI66932970, Length=188, Percent_Identity=40.9574468085106, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI66932972, Length=177, Percent_Identity=42.9378531073446, Blast_Score=140, Evalue=8e-34,
Organism=Escherichia coli, GI1788476, Length=176, Percent_Identity=36.3636363636364, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=45.5056179775281, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321706, Length=177, Percent_Identity=45.7627118644068, Blast_Score=167, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24656782, Length=175, Percent_Identity=47.4285714285714, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24656791, Length=175, Percent_Identity=47.4285714285714, Blast_Score=161, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24656796, Length=175, Percent_Identity=47.4285714285714, Blast_Score=161, Evalue=4e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 26380; Mature: 26380

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLIKTLRANKPEGKPSETKLAGERPAELDPAEFLAAAVRADRPRPSRAEAEAAVYTLL
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
NYIGEDPTREGLIDTPRRVVEAYDELFQGYHQCPAEVLDRTFGETAGYDDFVLIRDIEFT
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCHH
SQCEHHMMPFYGRAHIAYTPVERVVGLSKLARLTDIFARRLQTQEHLTAQIAAAIDEVLK
HHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PRGVAVMIEAEHTCMSVRGVAKHGAVTFTSRFTGMFRDNPAEQSRFLSMVRSPQR
CCCCEEEEECCHHHHHHHHHHHCCCEEEHHHHHHHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDTLIKTLRANKPEGKPSETKLAGERPAELDPAEFLAAAVRADRPRPSRAEAEAAVYTLL
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
NYIGEDPTREGLIDTPRRVVEAYDELFQGYHQCPAEVLDRTFGETAGYDDFVLIRDIEFT
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCHH
SQCEHHMMPFYGRAHIAYTPVERVVGLSKLARLTDIFARRLQTQEHLTAQIAAAIDEVLK
HHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PRGVAVMIEAEHTCMSVRGVAKHGAVTFTSRFTGMFRDNPAEQSRFLSMVRSPQR
CCCCEEEEECCHHHHHHHHHHHCCCEEEHHHHHHHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]