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The map label for this gene is yfcG [H]

Identifier: 146339908

GI number: 146339908

Start: 3111598

End: 3112302

Strand: Direct

Name: yfcG [H]

Synonym: BRADO2912

Alternate gene names: 146339908

Gene position: 3111598-3112302 (Clockwise)

Preceding gene: 146339906

Following gene: 146339909

Centisome position: 41.73

GC content: 64.11

Gene sequence:

>705_bases
ATGATCGACCTCCATTACGCGCCGACGCCGAACGGCTGGAAGATCTCGATCATGCTCGAGGAACTCGGGCTGCCTTATAC
CGTCATTCCCGTCGACATCCGTGCCGGAGACCAGTTCAAGCCGGATTTCCTGGCGATCAGCCCGAACAACCGGATTCCGG
CGATCATCGACCACGCTCCGGCAGACGGGGGCGAGCCGTTGTCGGTGTTCGAGACCGGCGCGATCCTGATCTACCTCGCC
GACAAGACCGGGCGTTTTCTGCCAACGGAAATGCGCGCGCGCTCGCGCGTGGTGCAATGGGTGATGTGGCAGATGGCGGG
GCTCGGGCCGATGATGGGCCAGCACGGTCATTTCGCGCTGTACGCTGCGGAGAAAGTGCCCTACGCGATCCAGCGCTACC
GCGACGAGGTCGCGCGCCTGTTCCACGTGCTCGACGTGCAGCTCGGCAAGACCGGCGCCTACGTCGCGGGTGAGGACTAC
TCGATTGCCGACATCGCCTGCTTCCCGTGGATGATGACGCACAAGGCGCAGGGCTTCACGCTCGACGACTATCCGAACAT
CAAGCGCTGGTACGCAACCTTGCGCGCGCGGCCGAAGCTGCAGGCGGGTCTTGCGATCGGCAAGTTCGTGAAGGAGCCGT
TCAACGACGAGACGCGCCACGTGATGTTCGGCGCGCGGGCGAAGGAGATGACGGGGCGATCGTGA

Upstream 100 bases:

>100_bases
TGGCTTAACTGTCAGGTACAGAAAGAACGGAGGCCGGACACGCGTTCCGCAGCGCGGCATAAGGGACCGGACACGATTGG
GGAAGGATGAAGGGAGAGGC

Downstream 100 bases:

>100_bases
GCCAGCGCCACGGACGCATCATGGGCGTTACGATAAACCCATCCAGACCGGCCGTGTCGCCCCTGGATTGCTTCGTCGCG
GAGCCTGTCATCGGGCCGCG

Product: putative glutathione transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MIDLHYAPTPNGWKISIMLEELGLPYTVIPVDIRAGDQFKPDFLAISPNNRIPAIIDHAPADGGEPLSVFETGAILIYLA
DKTGRFLPTEMRARSRVVQWVMWQMAGLGPMMGQHGHFALYAAEKVPYAIQRYRDEVARLFHVLDVQLGKTGAYVAGEDY
SIADIACFPWMMTHKAQGFTLDDYPNIKRWYATLRARPKLQAGLAIGKFVKEPFNDETRHVMFGARAKEMTGRS

Sequences:

>Translated_234_residues
MIDLHYAPTPNGWKISIMLEELGLPYTVIPVDIRAGDQFKPDFLAISPNNRIPAIIDHAPADGGEPLSVFETGAILIYLA
DKTGRFLPTEMRARSRVVQWVMWQMAGLGPMMGQHGHFALYAAEKVPYAIQRYRDEVARLFHVLDVQLGKTGAYVAGEDY
SIADIACFPWMMTHKAQGFTLDDYPNIKRWYATLRARPKLQAGLAIGKFVKEPFNDETRHVMFGARAKEMTGRS
>Mature_234_residues
MIDLHYAPTPNGWKISIMLEELGLPYTVIPVDIRAGDQFKPDFLAISPNNRIPAIIDHAPADGGEPLSVFETGAILIYLA
DKTGRFLPTEMRARSRVVQWVMWQMAGLGPMMGQHGHFALYAAEKVPYAIQRYRDEVARLFHVLDVQLGKTGAYVAGEDY
SIADIACFPWMMTHKAQGFTLDDYPNIKRWYATLRARPKLQAGLAIGKFVKEPFNDETRHVMFGARAKEMTGRS

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=198, Percent_Identity=56.0606060606061, Blast_Score=240, Evalue=5e-65,
Organism=Escherichia coli, GI87082195, Length=230, Percent_Identity=36.9565217391304, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI87081789, Length=189, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324100, Length=233, Percent_Identity=31.7596566523605, Blast_Score=123, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28571670, Length=177, Percent_Identity=27.1186440677966, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17864594, Length=198, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17864592, Length=194, Percent_Identity=28.8659793814433, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24646249, Length=201, Percent_Identity=29.3532338308458, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17933730, Length=207, Percent_Identity=27.0531400966184, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI85725204, Length=199, Percent_Identity=25.1256281407035, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17737923, Length=199, Percent_Identity=25.1256281407035, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24646251, Length=200, Percent_Identity=28, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26343; Mature: 26343

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLHYAPTPNGWKISIMLEELGLPYTVIPVDIRAGDQFKPDFLAISPNNRIPAIIDHAP
CEEEEECCCCCCEEEEEEEHHHCCCEEEEEEEEECCCCCCCCEEEECCCCCCCEEEECCC
ADGGEPLSVFETGAILIYLADKTGRFLPTEMRARSRVVQWVMWQMAGLGPMMGQHGHFAL
CCCCCCEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
YAAEKVPYAIQRYRDEVARLFHVLDVQLGKTGAYVAGEDYSIADIACFPWMMTHKAQGFT
EECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCCCEEHHHHHHHHHHCCCCCCC
LDDYPNIKRWYATLRARPKLQAGLAIGKFVKEPFNDETRHVMFGARAKEMTGRS
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHCCCCC
>Mature Secondary Structure
MIDLHYAPTPNGWKISIMLEELGLPYTVIPVDIRAGDQFKPDFLAISPNNRIPAIIDHAP
CEEEEECCCCCCEEEEEEEHHHCCCEEEEEEEEECCCCCCCCEEEECCCCCCCEEEECCC
ADGGEPLSVFETGAILIYLADKTGRFLPTEMRARSRVVQWVMWQMAGLGPMMGQHGHFAL
CCCCCCEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
YAAEKVPYAIQRYRDEVARLFHVLDVQLGKTGAYVAGEDYSIADIACFPWMMTHKAQGFT
EECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCCCEEHHHHHHHHHHCCCCCCC
LDDYPNIKRWYATLRARPKLQAGLAIGKFVKEPFNDETRHVMFGARAKEMTGRS
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]