BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is livG [H]

Identifier: 146339848

GI number: 146339848

Start: 3052315

End: 3053148

Strand: Reverse

Name: livG [H]

Synonym: BRADO2850

Alternate gene names: 146339848

Gene position: 3053148-3052315 (Counterclockwise)

Preceding gene: 146339849

Following gene: 146339847

Centisome position: 40.95

GC content: 65.47

Gene sequence:

>834_bases
ATGAGCTCCGCTCCCATCCTCGCGCTCGAGAATCTCACCATGCGTTTCGGCGGCATCGTCGCCGTCAACGCGCTGTCGTT
CACCGCCCAGCGCAAGCAGATCACGGCGCTGATCGGCCCCAACGGCGCCGGCAAGACCACTGTCTTCAACTGCATCACGG
GCTTCTACAAGCCGACCTCCGGCGTGATCCGCCTGACGCATGACGACGGCACGCAATTCGAGATGCAGAAGCTGAAGGAC
TTCAACATCTCCAAGCAGGCCAAGGTCGCGCGCACCTTCCAGAACATCCGGCTGTTTCCCGGCATGACGGCGCTCGAGAA
CCTGATGGTGGCGCAGCACAACGCCCTGATGCGCGCCTCCGGTCTGACATTGATCGGGCTGCTCGGCCTGCCATCCTGGC
GCGCGGCCGAGCAGAGCGCGATCGACAATGCGGTGTTCTGGCTGAAGCGCGTCGGCCTGATCGACCGCGCCGACGACGCC
GCCGGCAATCTCGCTTATGGCGATCAGCGCCGTCTCGAGATCGCGCGCGCGATGTGCACCGGGCCTGCCCTGCTCTGCCT
CGACGAGCCGGCCGCCGGCCTCAACGCCCGCGAAAGCGCGGCGTTGAGCGAGCTGCTTCTCTCGATCCGCAGCGACCACG
CCACATCGATCCTGCTGATCGAGCACGACATGAGCGTGGTGATGGAGATCTCCGACCGCGTCGTGGTGATGGACTATGGC
GTCAAGATCGCCGAGGGAACGCCGCGCGAGATCCGCGACGATCCGAAGGTGATCGCCGCCTATCTCGGCACCGACGAGGA
AGAAGCCGCAGCCGTGATGGAGGCCGAGGCGTGA

Upstream 100 bases:

>100_bases
TGATCTGGCGCCCGCGCGGCATCGTCGGCCATCGCGCGCCGACCGTGTTCCTGGAAAAATCCAAGAGCATCTCCTCGGAC
CTCGTCAAGGAAGGCCACGG

Downstream 100 bases:

>100_bases
GTGCATCCCTGCTCGCGATCCGTAGCCTGCGCGCCTCCTACGGCAAGATCGAAGCGCTGAAGGGCGTCGACCTCGACATC
AAGGCCGGCGAAATCGTCGC

Product: high-affinity branched-chain amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSSAPILALENLTMRFGGIVAVNALSFTAQRKQITALIGPNGAGKTTVFNCITGFYKPTSGVIRLTHDDGTQFEMQKLKD
FNISKQAKVARTFQNIRLFPGMTALENLMVAQHNALMRASGLTLIGLLGLPSWRAAEQSAIDNAVFWLKRVGLIDRADDA
AGNLAYGDQRRLEIARAMCTGPALLCLDEPAAGLNARESAALSELLLSIRSDHATSILLIEHDMSVVMEISDRVVVMDYG
VKIAEGTPREIRDDPKVIAAYLGTDEEEAAAVMEAEA

Sequences:

>Translated_277_residues
MSSAPILALENLTMRFGGIVAVNALSFTAQRKQITALIGPNGAGKTTVFNCITGFYKPTSGVIRLTHDDGTQFEMQKLKD
FNISKQAKVARTFQNIRLFPGMTALENLMVAQHNALMRASGLTLIGLLGLPSWRAAEQSAIDNAVFWLKRVGLIDRADDA
AGNLAYGDQRRLEIARAMCTGPALLCLDEPAAGLNARESAALSELLLSIRSDHATSILLIEHDMSVVMEISDRVVVMDYG
VKIAEGTPREIRDDPKVIAAYLGTDEEEAAAVMEAEA
>Mature_276_residues
SSAPILALENLTMRFGGIVAVNALSFTAQRKQITALIGPNGAGKTTVFNCITGFYKPTSGVIRLTHDDGTQFEMQKLKDF
NISKQAKVARTFQNIRLFPGMTALENLMVAQHNALMRASGLTLIGLLGLPSWRAAEQSAIDNAVFWLKRVGLIDRADDAA
GNLAYGDQRRLEIARAMCTGPALLCLDEPAAGLNARESAALSELLLSIRSDHATSILLIEHDMSVVMEISDRVVVMDYGV
KIAEGTPREIRDDPKVIAAYLGTDEEEAAAVMEAEA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=240, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI27881501, Length=247, Percent_Identity=25.9109311740891, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI30795238, Length=247, Percent_Identity=25.9109311740891, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI21536376, Length=237, Percent_Identity=29.1139240506329, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI105990541, Length=252, Percent_Identity=26.5873015873016, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI116734710, Length=221, Percent_Identity=29.8642533936652, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI9665248, Length=236, Percent_Identity=27.1186440677966, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI47078218, Length=231, Percent_Identity=27.7056277056277, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI45446740, Length=231, Percent_Identity=27.7056277056277, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI27436953, Length=224, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1789864, Length=261, Percent_Identity=53.639846743295, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI87081709, Length=265, Percent_Identity=27.9245283018868, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789672, Length=253, Percent_Identity=26.0869565217391, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790525, Length=252, Percent_Identity=26.5873015873016, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787370, Length=275, Percent_Identity=28.3636363636364, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788761, Length=260, Percent_Identity=28.8461538461538, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788225, Length=257, Percent_Identity=27.2373540856031, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786872, Length=253, Percent_Identity=26.4822134387352, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788210, Length=263, Percent_Identity=27.7566539923954, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787029, Length=250, Percent_Identity=24.8, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789032, Length=249, Percent_Identity=26.9076305220884, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786398, Length=257, Percent_Identity=30.7392996108949, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081791, Length=262, Percent_Identity=27.8625954198473, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789593, Length=259, Percent_Identity=26.6409266409266, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786563, Length=268, Percent_Identity=27.9850746268657, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790190, Length=243, Percent_Identity=27.1604938271605, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI48995001, Length=237, Percent_Identity=27.0042194092827, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994997, Length=257, Percent_Identity=26.4591439688716, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082268, Length=260, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082267, Length=261, Percent_Identity=26.8199233716475, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787712, Length=258, Percent_Identity=24.8062015503876, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI48994943, Length=270, Percent_Identity=28.5185185185185, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI87081834, Length=242, Percent_Identity=28.099173553719, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1789991, Length=244, Percent_Identity=27.0491803278689, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788644, Length=259, Percent_Identity=27.7992277992278, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787164, Length=234, Percent_Identity=27.3504273504274, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081782, Length=249, Percent_Identity=26.9076305220884, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790739, Length=246, Percent_Identity=28.0487804878049, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788450, Length=268, Percent_Identity=25, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786703, Length=212, Percent_Identity=26.4150943396226, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790544, Length=277, Percent_Identity=25.2707581227437, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790467, Length=260, Percent_Identity=23.0769230769231, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786345, Length=248, Percent_Identity=27.4193548387097, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790162, Length=239, Percent_Identity=26.7782426778243, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787089, Length=243, Percent_Identity=25.1028806584362, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787758, Length=238, Percent_Identity=26.0504201680672, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789962, Length=239, Percent_Identity=24.6861924686192, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI145693107, Length=269, Percent_Identity=26.7657992565056, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786803, Length=247, Percent_Identity=24.2914979757085, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789873, Length=228, Percent_Identity=26.7543859649123, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788897, Length=265, Percent_Identity=26.4150943396226, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787792, Length=254, Percent_Identity=25.5905511811024, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786253, Length=254, Percent_Identity=26.7716535433071, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787105, Length=243, Percent_Identity=25.5144032921811, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788506, Length=239, Percent_Identity=26.3598326359833, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787759, Length=269, Percent_Identity=27.8810408921933, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788472, Length=252, Percent_Identity=21.4285714285714, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789963, Length=263, Percent_Identity=25.8555133079848, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790534, Length=218, Percent_Identity=27.5229357798165, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994883, Length=257, Percent_Identity=24.9027237354086, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787500, Length=241, Percent_Identity=24.896265560166, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789891, Length=220, Percent_Identity=27.7272727272727, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=233, Percent_Identity=28.755364806867, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115533608, Length=222, Percent_Identity=29.2792792792793, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17567269, Length=226, Percent_Identity=29.646017699115, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983523, Length=246, Percent_Identity=28.8617886178862, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71983510, Length=246, Percent_Identity=28.8617886178862, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71983516, Length=246, Percent_Identity=28.8617886178862, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17565586, Length=233, Percent_Identity=27.8969957081545, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24666092, Length=233, Percent_Identity=31.3304721030043, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI221512771, Length=242, Percent_Identity=28.9256198347107, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648314, Length=254, Percent_Identity=25.1968503937008, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28574150, Length=235, Percent_Identity=27.6595744680851, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI116007328, Length=235, Percent_Identity=27.6595744680851, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28574533, Length=219, Percent_Identity=28.310502283105, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI116007184, Length=241, Percent_Identity=26.1410788381743, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221500365, Length=241, Percent_Identity=26.1410788381743, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19920532, Length=231, Percent_Identity=23.8095238095238, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24580930, Length=231, Percent_Identity=23.8095238095238, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581191, Length=222, Percent_Identity=26.1261261261261, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581189, Length=222, Percent_Identity=26.1261261261261, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581193, Length=222, Percent_Identity=26.1261261261261, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581195, Length=222, Percent_Identity=26.1261261261261, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 29935; Mature: 29804

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAPILALENLTMRFGGIVAVNALSFTAQRKQITALIGPNGAGKTTVFNCITGFYKPTS
CCCCCEEEEHHHHHHHCCEEEEEHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCC
GVIRLTHDDGTQFEMQKLKDFNISKQAKVARTFQNIRLFPGMTALENLMVAQHNALMRAS
CEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHCEECCCHHHHHHHHHHHHHHHHHHC
GLTLIGLLGLPSWRAAEQSAIDNAVFWLKRVGLIDRADDAAGNLAYGDQRRLEIARAMCT
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
GPALLCLDEPAAGLNARESAALSELLLSIRSDHATSILLIEHDMSVVMEISDRVVVMDYG
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCEEEEECC
VKIAEGTPREIRDDPKVIAAYLGTDEEEAAAVMEAEA
EEEECCCCHHHCCCHHEEEEECCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SSAPILALENLTMRFGGIVAVNALSFTAQRKQITALIGPNGAGKTTVFNCITGFYKPTS
CCCCEEEEHHHHHHHCCEEEEEHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCC
GVIRLTHDDGTQFEMQKLKDFNISKQAKVARTFQNIRLFPGMTALENLMVAQHNALMRAS
CEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHCEECCCHHHHHHHHHHHHHHHHHHC
GLTLIGLLGLPSWRAAEQSAIDNAVFWLKRVGLIDRADDAAGNLAYGDQRRLEIARAMCT
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
GPALLCLDEPAAGLNARESAALSELLLSIRSDHATSILLIEHDMSVVMEISDRVVVMDYG
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCEEEEECC
VKIAEGTPREIRDDPKVIAAYLGTDEEEAAAVMEAEA
EEEECCCCHHHCCCHHEEEEECCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]