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The map label for this gene is livK [C]

Identifier: 146339846

GI number: 146339846

Start: 3050312

End: 3051430

Strand: Reverse

Name: livK [C]

Synonym: BRADO2848

Alternate gene names: 146339846

Gene position: 3051430-3050312 (Counterclockwise)

Preceding gene: 146339847

Following gene: 146339844

Centisome position: 40.92

GC content: 64.16

Gene sequence:

>1119_bases
ATGAAACCACTCAAACTCATTGGTCTGGCCCTGGGCGCCACGCTTGCGCTCTCGACCGCGGCCTACGCCGAAGACATCAC
CATCGCCGTGGCCGGCCCGATGACCGGCGGCGAGTCGGCCTTCGGCCGCCAGATGCAGAACGGCGCCGAGATGGCGATCG
CCGATCTCAATGCCGCCGGCGGCGTGCTCGGCAAGAAGCTCGCGCTGCAGGTGGGTGACGATGCCTGCGATCCGAAGCAG
GCCCGCTCGGTCGCCGAGAAGCTCGCCGGCTCCGGCATTCCGTTCGTCGCCGGCCATTTCTGCTCGTCGTCATCGATCCC
GGCCTCGGAGGCCTATGCCGACAGCAACGTGCTGCAGATCACCCCGGCTTCGACCAACCCGCTGTTCACCGAGCGCAAGC
TGTGGAACGTCGCCCGCGTCTGCGGCCGTGACGATCAGCAGGGCCTCGTCGCCGCCAACTACATCGCGAAGAACTTCAAG
GGCAAGAACATCGCGATCCTCAACGACAAGACCACCTACGGCAAGGGTCTCGCCGACGAAACCAAGAAGGCGCTCAACAA
GGCCGGCGTCACCGAGAAGATGTTCGAGTCGTACAACAAGGGCGACAAGGACTTCAACGCGATCGTGTCGCGCCTGAAGC
GTGAGAACATCGACCTCGTCTATGTCGGCGGCTATCATCAGGAAGCCGGTCTGATCCTGCGCCAGATGCGCGACCAGGGC
CTGAAGACGATCCTGATGGCGGGCGACGCCATGAACGACAAGGAGTTCGCCTCCATCACCGGTCCGGCCGCTGAGGGCAC
CCTCTTCACCTTCGGCCCCGAGCCGCGCAACAAGCCGACCGCGAAGGCGATCGTCGAGAAGTTCAAGGCCAAGAACATCG
ACCCCGAAGGCTACACCCTCTACACCTACGCCGCGATCCAGGTCTGGTCGCAGGCGGTGAAGAAGGCCAACACCACCGAC
GCCAAGAAGGTGATGGACACCATCAAGGCGGGCGAGTGGGACACCGTGCTCGGCAAGCTCGGCTTCGACGCCAAGGGCGA
CATCAAGCAGATCGACTACGTCGTCTACAAGTGGGACGCCAAGGGCGGCTACGCCGAGCTCGGCGGCGGCAAGTCCTGA

Upstream 100 bases:

>100_bases
AAAATGCCGGTGACTTCGGCGGAAAATCAGCCACAATGCGCGCGGTCTGCGGCCCGCCATTCGGGCCATTTCCATGTCGA
CCTACCCGCGAGGATTTCTC

Downstream 100 bases:

>100_bases
TCAGGTCCGCTGATTGCGGTTACGTCAAATGCAAACGCCCCGGTCATGGACCGGGGCGTTCTGTTTTGACTTTGGCGATG
CGGACACCGACGTCCGCGTC

Product: high-affinity branched-chain amino acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKPLKLIGLALGATLALSTAAYAEDITIAVAGPMTGGESAFGRQMQNGAEMAIADLNAAGGVLGKKLALQVGDDACDPKQ
ARSVAEKLAGSGIPFVAGHFCSSSSIPASEAYADSNVLQITPASTNPLFTERKLWNVARVCGRDDQQGLVAANYIAKNFK
GKNIAILNDKTTYGKGLADETKKALNKAGVTEKMFESYNKGDKDFNAIVSRLKRENIDLVYVGGYHQEAGLILRQMRDQG
LKTILMAGDAMNDKEFASITGPAAEGTLFTFGPEPRNKPTAKAIVEKFKAKNIDPEGYTLYTYAAIQVWSQAVKKANTTD
AKKVMDTIKAGEWDTVLGKLGFDAKGDIKQIDYVVYKWDAKGGYAELGGGKS

Sequences:

>Translated_372_residues
MKPLKLIGLALGATLALSTAAYAEDITIAVAGPMTGGESAFGRQMQNGAEMAIADLNAAGGVLGKKLALQVGDDACDPKQ
ARSVAEKLAGSGIPFVAGHFCSSSSIPASEAYADSNVLQITPASTNPLFTERKLWNVARVCGRDDQQGLVAANYIAKNFK
GKNIAILNDKTTYGKGLADETKKALNKAGVTEKMFESYNKGDKDFNAIVSRLKRENIDLVYVGGYHQEAGLILRQMRDQG
LKTILMAGDAMNDKEFASITGPAAEGTLFTFGPEPRNKPTAKAIVEKFKAKNIDPEGYTLYTYAAIQVWSQAVKKANTTD
AKKVMDTIKAGEWDTVLGKLGFDAKGDIKQIDYVVYKWDAKGGYAELGGGKS
>Mature_372_residues
MKPLKLIGLALGATLALSTAAYAEDITIAVAGPMTGGESAFGRQMQNGAEMAIADLNAAGGVLGKKLALQVGDDACDPKQ
ARSVAEKLAGSGIPFVAGHFCSSSSIPASEAYADSNVLQITPASTNPLFTERKLWNVARVCGRDDQQGLVAANYIAKNFK
GKNIAILNDKTTYGKGLADETKKALNKAGVTEKMFESYNKGDKDFNAIVSRLKRENIDLVYVGGYHQEAGLILRQMRDQG
LKTILMAGDAMNDKEFASITGPAAEGTLFTFGPEPRNKPTAKAIVEKFKAKNIDPEGYTLYTYAAIQVWSQAVKKANTTD
AKKVMDTIKAGEWDTVLGKLGFDAKGDIKQIDYVVYKWDAKGGYAELGGGKS

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=349, Percent_Identity=42.9799426934097, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI48994941, Length=358, Percent_Identity=44.4134078212291, Blast_Score=281, Evalue=3e-77,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39685; Mature: 39685

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLKLIGLALGATLALSTAAYAEDITIAVAGPMTGGESAFGRQMQNGAEMAIADLNAAG
CCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHC
GVLGKKLALQVGDDACDPKQARSVAEKLAGSGIPFVAGHFCSSSSIPASEAYADSNVLQI
CHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEHHCCCCCCCCCHHHHCCCCEEEE
TPASTNPLFTERKLWNVARVCGRDDQQGLVAANYIAKNFKGKNIAILNDKTTYGKGLADE
ECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHH
TKKALNKAGVTEKMFESYNKGDKDFNAIVSRLKRENIDLVYVGGYHQEAGLILRQMRDQG
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCC
LKTILMAGDAMNDKEFASITGPAAEGTLFTFGPEPRNKPTAKAIVEKFKAKNIDPEGYTL
CEEEEEECCCCCCCHHHEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEE
YTYAAIQVWSQAVKKANTTDAKKVMDTIKAGEWDTVLGKLGFDAKGDIKQIDYVVYKWDA
EHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEC
KGGYAELGGGKS
CCCEECCCCCCC
>Mature Secondary Structure
MKPLKLIGLALGATLALSTAAYAEDITIAVAGPMTGGESAFGRQMQNGAEMAIADLNAAG
CCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHC
GVLGKKLALQVGDDACDPKQARSVAEKLAGSGIPFVAGHFCSSSSIPASEAYADSNVLQI
CHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEHHCCCCCCCCCHHHHCCCCEEEE
TPASTNPLFTERKLWNVARVCGRDDQQGLVAANYIAKNFKGKNIAILNDKTTYGKGLADE
ECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHH
TKKALNKAGVTEKMFESYNKGDKDFNAIVSRLKRENIDLVYVGGYHQEAGLILRQMRDQG
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCC
LKTILMAGDAMNDKEFASITGPAAEGTLFTFGPEPRNKPTAKAIVEKFKAKNIDPEGYTL
CEEEEEECCCCCCCHHHEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEE
YTYAAIQVWSQAVKKANTTDAKKVMDTIKAGEWDTVLGKLGFDAKGDIKQIDYVVYKWDA
EHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEC
KGGYAELGGGKS
CCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA