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The map label for this gene is purB [H]

Identifier: 146339829

GI number: 146339829

Start: 3032477

End: 3033784

Strand: Reverse

Name: purB [H]

Synonym: BRADO2830

Alternate gene names: 146339829

Gene position: 3033784-3032477 (Counterclockwise)

Preceding gene: 146339839

Following gene: 146339826

Centisome position: 40.69

GC content: 66.82

Gene sequence:

>1308_bases
ATGATCCCCCGCTACACCCGTCCCGAAATGGCCTCGATCTGGGAACCGCAGACCCGGTTCAAGATCTGGTTCGAGATCGA
GGCGCATGCGGCGGACGCGCTGGCCGAGCTCGGCACCATACCCAAGGATGCCGCCAGGACGATCTGGGCCAAGGCCAAGG
ACGCTACTTTCGACGTCGCCCGCATCGACGAGATCGAGCGCGAGACCAAGCACGACGTCATCGCCTTCCTGACCCATCTC
GCCGAGATCGTCGGTCCCGAGGCGCGCTTCGTGCACCAGGGCATGACCTCGTCCGACGTGCTCGACACCTGCCTCAACGT
GCAGCTGACCCGCGCCGCCGACCTGCTGCTCGCCGATCTCGACAAGGTTCTCGCGGCGCTCAAGAAGCGCGCCTTCGAGC
ACAAGATGACGCCGACCATCGGCCGGTCGCACGGCATCCATGCCGAGCCGGTCACCTTCGGCCTGAAGCTCGCTTATGCC
TATGCCGAGTTCTCCCGCGCCCGTGACCGCCTGGTCGCCGCGCGCAAGGAGGTCGCGACCTGCGCCATCTCGGGCGCGGT
CGGCACATTCGCGCAGATCGATCCGCGCGTGGAGGAGCACGTTGCCAAGGCGATGGGTCTGACGCCCGAGCCGGTGTCGA
CCCAGGTCATCCCGCGCGATCGCCACGCGATGTTCTTCGCCACGCTCGGCGTGATCGCCTCCTCCGTCGAGCGCTTCGCG
ACGGAAGTTCGCCACCTGCAGCGCACCGAGGTGCTGGAGGCCGAGGAGTTCTTCTCGGAGGGCCAGAAGGGCTCCTCGGC
GATGCCGCACAAGCGCAACCCGGTGCTGTCGGAGAACCTGACCGGCCTCGCCCGCATGGTCCGCGCCTATGTGACGCCGG
CGATGGAGAACGTCGTGCTCTGGCACGAGCGCGACATCTCGCACTCCTCGGTCGAGCGCATGATCGGGCCCGACGCCACC
GTCACGCTGGACTTCGCGCTGGTGCGCCTCGCCGGCCTGACCGAGAAGCTCCTGGTCTACCCGGCCAACATGGAGAAGAA
CCTCGACCGGCTCGGCGGCCTCGTGCATTCGCAGCGCCTCTTGATCGCGCTCACCCAGAAGGGCGCGAGTCGCGAGGACT
CCTACAAGCTGGTCCAGCGCAACGCGATGCCGGTGTGGCGCGGCGAAGGCGACTTCCGCACCCTGCTGAAGGCCGACGCC
GACGTGAAGAAGTATCTGACCGATGCCGAGATCGACGAGCAGTTCGACCTCGGCTACCACCTCAAGCACGTCGACACGAT
CTTCAAGCGGGTGTTCGGCGAGAGCTGA

Upstream 100 bases:

>100_bases
TTGTGTCGCCTCGGCGGCTCTGGTAGAGCACGGCGTAAAATGCTGCTGGCGCGGACGCGTTTCGTCCCGCCTACCTTCCC
TCCCGCCTGGAGAGCCGTCG

Downstream 100 bases:

>100_bases
GCGCGCCTCGATCAGGCGGACGCCTCAGTCCGCCTCCTCGCACTTGTCCTGCTTGACCTTGAACATCTTCCGGAACTGGG
TCTCGAGCGCGCTCCGCATC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIPRYTRPEMASIWEPQTRFKIWFEIEAHAADALAELGTIPKDAARTIWAKAKDATFDVARIDEIERETKHDVIAFLTHL
AEIVGPEARFVHQGMTSSDVLDTCLNVQLTRAADLLLADLDKVLAALKKRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYA
YAEFSRARDRLVAARKEVATCAISGAVGTFAQIDPRVEEHVAKAMGLTPEPVSTQVIPRDRHAMFFATLGVIASSVERFA
TEVRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLSENLTGLARMVRAYVTPAMENVVLWHERDISHSSVERMIGPDAT
VTLDFALVRLAGLTEKLLVYPANMEKNLDRLGGLVHSQRLLIALTQKGASREDSYKLVQRNAMPVWRGEGDFRTLLKADA
DVKKYLTDAEIDEQFDLGYHLKHVDTIFKRVFGES

Sequences:

>Translated_435_residues
MIPRYTRPEMASIWEPQTRFKIWFEIEAHAADALAELGTIPKDAARTIWAKAKDATFDVARIDEIERETKHDVIAFLTHL
AEIVGPEARFVHQGMTSSDVLDTCLNVQLTRAADLLLADLDKVLAALKKRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYA
YAEFSRARDRLVAARKEVATCAISGAVGTFAQIDPRVEEHVAKAMGLTPEPVSTQVIPRDRHAMFFATLGVIASSVERFA
TEVRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLSENLTGLARMVRAYVTPAMENVVLWHERDISHSSVERMIGPDAT
VTLDFALVRLAGLTEKLLVYPANMEKNLDRLGGLVHSQRLLIALTQKGASREDSYKLVQRNAMPVWRGEGDFRTLLKADA
DVKKYLTDAEIDEQFDLGYHLKHVDTIFKRVFGES
>Mature_435_residues
MIPRYTRPEMASIWEPQTRFKIWFEIEAHAADALAELGTIPKDAARTIWAKAKDATFDVARIDEIERETKHDVIAFLTHL
AEIVGPEARFVHQGMTSSDVLDTCLNVQLTRAADLLLADLDKVLAALKKRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYA
YAEFSRARDRLVAARKEVATCAISGAVGTFAQIDPRVEEHVAKAMGLTPEPVSTQVIPRDRHAMFFATLGVIASSVERFA
TEVRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLSENLTGLARMVRAYVTPAMENVVLWHERDISHSSVERMIGPDAT
VTLDFALVRLAGLTEKLLVYPANMEKNLDRLGGLVHSQRLLIALTQKGASREDSYKLVQRNAMPVWRGEGDFRTLLKADA
DVKKYLTDAEIDEQFDLGYHLKHVDTIFKRVFGES

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=402, Percent_Identity=28.3582089552239, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=28.6089238845144, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1787376, Length=352, Percent_Identity=28.4090909090909, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=399, Percent_Identity=26.3157894736842, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=365, Percent_Identity=27.3972602739726, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323391, Length=411, Percent_Identity=28.2238442822384, Blast_Score=136, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24647570, Length=382, Percent_Identity=26.9633507853403, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48694; Mature: 48694

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYTRPEMASIWEPQTRFKIWFEIEAHAADALAELGTIPKDAARTIWAKAKDATFDVA
CCCCCCCCCHHHHCCCCCEEEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHH
RIDEIERETKHDVIAFLTHLAEIVGPEARFVHQGMTSSDVLDTCLNVQLTRAADLLLADL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
DKVLAALKKRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYAYAEFSRARDRLVAARKEVAT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFAQIDPRVEEHVAKAMGLTPEPVSTQVIPRDRHAMFFATLGVIASSVERFA
HHHHCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEVRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLSENLTGLARMVRAYVTPAMENVVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
WHERDISHSSVERMIGPDATVTLDFALVRLAGLTEKLLVYPANMEKNLDRLGGLVHSQRL
EECCCCCHHHHHHHHCCCCEEHHHHHHHHHHCCCHHHEEECCCHHHHHHHHHHHHHHCHH
LIALTQKGASREDSYKLVQRNAMPVWRGEGDFRTLLKADADVKKYLTDAEIDEQFDLGYH
EEEEECCCCCCCHHHHHHHHCCCCEECCCCHHHHHHHCCHHHHHHHCCCCCCCHHCCCHH
LKHVDTIFKRVFGES
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPRYTRPEMASIWEPQTRFKIWFEIEAHAADALAELGTIPKDAARTIWAKAKDATFDVA
CCCCCCCCCHHHHCCCCCEEEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHH
RIDEIERETKHDVIAFLTHLAEIVGPEARFVHQGMTSSDVLDTCLNVQLTRAADLLLADL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
DKVLAALKKRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYAYAEFSRARDRLVAARKEVAT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFAQIDPRVEEHVAKAMGLTPEPVSTQVIPRDRHAMFFATLGVIASSVERFA
HHHHCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEVRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLSENLTGLARMVRAYVTPAMENVVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
WHERDISHSSVERMIGPDATVTLDFALVRLAGLTEKLLVYPANMEKNLDRLGGLVHSQRL
EECCCCCHHHHHHHHCCCCEEHHHHHHHHHHCCCHHHEEECCCHHHHHHHHHHHHHHCHH
LIALTQKGASREDSYKLVQRNAMPVWRGEGDFRTLLKADADVKKYLTDAEIDEQFDLGYH
EEEEECCCCCCCHHHHHHHHCCCCEECCCCHHHHHHHCCHHHHHHHCCCCCCCHHCCCHH
LKHVDTIFKRVFGES
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]