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The map label for this gene is ltaA [H]

Identifier: 146339795

GI number: 146339795

Start: 3003277

End: 3004344

Strand: Reverse

Name: ltaA [H]

Synonym: BRADO2796

Alternate gene names: 146339795

Gene position: 3004344-3003277 (Counterclockwise)

Preceding gene: 146339796

Following gene: 146339791

Centisome position: 40.29

GC content: 69.76

Gene sequence:

>1068_bases
ATGTATTACACCCCGCCGCAACGCGATCCCAAGGCGCCGCCCGTGCGCGTCAACCTGCTCTCGGATACGCAGACGCGCCC
GACCGCCGCGATGCGCGAGGCGATGGCGCGGGCCGAGGTCGGCGACGAGCAGATCGGCGACGATCCGACCGTGCACGAGC
TCACCGCCCGCGTCGCCGCCCTGCTCGGCAAGGAAGCCGCGGTGCTGATGCCCTCGGGCACGATGTGCAACGTCGCCGCG
ACCCTGGTGCATTGCCGTCCCGGTGACGAGATCCTGGCGCACGAGACCGCGCACATCATCTCGCGCGAGGGCGGCGCGCA
TGCCGCGCTCGGCGGTTTCCAGGTGACGCAACTGAAGGGGCCCGACGGCCAGTTCACGTCGGAGACGTTCCGGGCTGCGC
TGCATCCGCGCTCGCGCTACCAGCCGCCGCAGACCGTCGTCAGCGTCGAGCAGACCGCCAATATCGGCGGCGGCACGATC
TGGAAGAAGGCCGCCCTCGACGAGATCGTGGAGATCGCCAAGGCGAGCGGCATGGCGACGCACATGGACGGCGCGCGGCT
GCTGAATGCGACCGTGGCAACCGGCATCCCCGCCGCCGAGATGACCGCTGGCTGGGATTCCGCCTGGATCGATTTCTCCA
AGGGGCTGGGCGCGCCGATCGGCGGCGTGCTCGCCGGCAGCGCGGCATTCATTGATGAAGTGTGGCGCTGGAAGCAGCGG
CTCGGCGGCGCCATGCGCCAGGCCGGCGTGGTCGCGGCGGCCTGCATCTACGCGCTCGATCACCACGTCGACCGGCTCGC
CGAGGATCACGCCAATGCGCGGGCGCTGGCGCGCGGGCTGTCGCAGATTCACGGCATCGAGGTGCAGGAGCCGGAGACCA
ATCTCGTGTTCTTCAAGCCCGACGGCGCCGGCGTGCCCGGCGACAGGATGGTGAGCGAACTCCGCAAGCGCGGCGTGCTG
CTCGCGATGATGGACGGCCGCATCCGCGCCTGCACCCATCTCGATGTAACCGCAGCGATGATCGAGGAGATGGTCGGCCA
TGTGAGGGAGATCGTGCGGGGAGCATGA

Upstream 100 bases:

>100_bases
GCCGGACATCTGGCTCGCGGCGTGATCGCAGGGAGGCGCGTTTTGCGGGAGGGTCGCGAGTTGGGTAAGGTCCTGGCGTG
AAAGCCAACGAGGACTGCCG

Downstream 100 bases:

>100_bases
ACTCTTAGCCCGCATGAGCGCAACGACATGCGGCTTCACCGATAAGGCTCGTGAAACCCCGGATGTCGCTTTCGCTCATC
CGGGCTACGCAAGCGCCTCA

Product: putative L-allo-threonine aldolase

Products: Acetaldehyde; Glycine [C]

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MYYTPPQRDPKAPPVRVNLLSDTQTRPTAAMREAMARAEVGDEQIGDDPTVHELTARVAALLGKEAAVLMPSGTMCNVAA
TLVHCRPGDEILAHETAHIISREGGAHAALGGFQVTQLKGPDGQFTSETFRAALHPRSRYQPPQTVVSVEQTANIGGGTI
WKKAALDEIVEIAKASGMATHMDGARLLNATVATGIPAAEMTAGWDSAWIDFSKGLGAPIGGVLAGSAAFIDEVWRWKQR
LGGAMRQAGVVAAACIYALDHHVDRLAEDHANARALARGLSQIHGIEVQEPETNLVFFKPDGAGVPGDRMVSELRKRGVL
LAMMDGRIRACTHLDVTAAMIEEMVGHVREIVRGA

Sequences:

>Translated_355_residues
MYYTPPQRDPKAPPVRVNLLSDTQTRPTAAMREAMARAEVGDEQIGDDPTVHELTARVAALLGKEAAVLMPSGTMCNVAA
TLVHCRPGDEILAHETAHIISREGGAHAALGGFQVTQLKGPDGQFTSETFRAALHPRSRYQPPQTVVSVEQTANIGGGTI
WKKAALDEIVEIAKASGMATHMDGARLLNATVATGIPAAEMTAGWDSAWIDFSKGLGAPIGGVLAGSAAFIDEVWRWKQR
LGGAMRQAGVVAAACIYALDHHVDRLAEDHANARALARGLSQIHGIEVQEPETNLVFFKPDGAGVPGDRMVSELRKRGVL
LAMMDGRIRACTHLDVTAAMIEEMVGHVREIVRGA
>Mature_355_residues
MYYTPPQRDPKAPPVRVNLLSDTQTRPTAAMREAMARAEVGDEQIGDDPTVHELTARVAALLGKEAAVLMPSGTMCNVAA
TLVHCRPGDEILAHETAHIISREGGAHAALGGFQVTQLKGPDGQFTSETFRAALHPRSRYQPPQTVVSVEQTANIGGGTI
WKKAALDEIVEIAKASGMATHMDGARLLNATVATGIPAAEMTAGWDSAWIDFSKGLGAPIGGVLAGSAAFIDEVWRWKQR
LGGAMRQAGVVAAACIYALDHHVDRLAEDHANARALARGLSQIHGIEVQEPETNLVFFKPDGAGVPGDRMVSELRKRGVL
LAMMDGRIRACTHLDVTAAMIEEMVGHVREIVRGA

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=339, Percent_Identity=38.3480825958702, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI193206522, Length=346, Percent_Identity=32.6589595375723, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI193206520, Length=346, Percent_Identity=32.6589595375723, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI193206524, Length=346, Percent_Identity=32.6589595375723, Blast_Score=168, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6320789, Length=341, Percent_Identity=31.0850439882698, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21355439, Length=296, Percent_Identity=41.554054054054, Blast_Score=214, Evalue=9e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5 [C]

Molecular weight: Translated: 37836; Mature: 37836

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYTPPQRDPKAPPVRVNLLSDTQTRPTAAMREAMARAEVGDEQIGDDPTVHELTARVAA
CCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHH
LLGKEAAVLMPSGTMCNVAATLVHCRPGDEILAHETAHIISREGGAHAALGGFQVTQLKG
HHCCCCEEEECCCCHHHHHHHHEECCCCCHHHHHHHHHHHHCCCCCCEEECCEEEEEECC
PDGQFTSETFRAALHPRSRYQPPQTVVSVEQTANIGGGTIWKKAALDEIVEIAKASGMAT
CCCCCHHHHHHHHHCCHHCCCCCHHHEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEE
HMDGARLLNATVATGIPAAEMTAGWDSAWIDFSKGLGAPIGGVLAGSAAFIDEVWRWKQR
CCCHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGGAMRQAGVVAAACIYALDHHVDRLAEDHANARALARGLSQIHGIEVQEPETNLVFFKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECC
DGAGVPGDRMVSELRKRGVLLAMMDGRIRACTHLDVTAAMIEEMVGHVREIVRGA
CCCCCCHHHHHHHHHHCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYYTPPQRDPKAPPVRVNLLSDTQTRPTAAMREAMARAEVGDEQIGDDPTVHELTARVAA
CCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHH
LLGKEAAVLMPSGTMCNVAATLVHCRPGDEILAHETAHIISREGGAHAALGGFQVTQLKG
HHCCCCEEEECCCCHHHHHHHHEECCCCCHHHHHHHHHHHHCCCCCCEEECCEEEEEECC
PDGQFTSETFRAALHPRSRYQPPQTVVSVEQTANIGGGTIWKKAALDEIVEIAKASGMAT
CCCCCHHHHHHHHHCCHHCCCCCHHHEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEE
HMDGARLLNATVATGIPAAEMTAGWDSAWIDFSKGLGAPIGGVLAGSAAFIDEVWRWKQR
CCCHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGGAMRQAGVVAAACIYALDHHVDRLAEDHANARALARGLSQIHGIEVQEPETNLVFFKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECC
DGAGVPGDRMVSELRKRGVLLAMMDGRIRACTHLDVTAAMIEEMVGHVREIVRGA
CCCCCCHHHHHHHHHHCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-Threonine [C]

Specific reaction: L-Threonine <==> Acetaldehyde + Glycine [C]

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]