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The map label for this gene is mutB [H]

Identifier: 146339684

GI number: 146339684

Start: 2879902

End: 2882058

Strand: Reverse

Name: mutB [H]

Synonym: BRADO2679

Alternate gene names: 146339684

Gene position: 2882058-2879902 (Counterclockwise)

Preceding gene: 146339685

Following gene: 146339683

Centisome position: 38.65

GC content: 66.57

Gene sequence:

>2157_bases
ATGACCCGCATTCCGAATTTCGCTGACATCGCGTTCGAACCCACCGCCGCCCCCGCGCCCGCCGGCGCCGCAGAGCCGTG
GCTGACGCCGGAGGGCATCCCGGTGAAGCCGGCTTATGGCGAGGCCGATCTCGCCGGCATCGATTTCCTGGAGACATGGC
CGGGCACAGCGCCGTATCTGCGCGGCCCCTACCCGACCATGTATGTCAACCAGCCCTGGACGGTCCGGCAATATGCCGGC
TTCTCCACGGCCGAGGATTCCAACGCGTTCTACCGGCGCAACCTCGCCGCGGGCCAGAAGGGTCTCTCGGTCGCGTTCGA
TCTCGCCACCCACCGCGGCTATGATTCCGATCATCCGCGCGTCGGCGGCGACGTCGGCATGGCCGGCGTCGCGATCGACT
CGATCTACGACATGCGCACCTTGTTCGCCGGCATCCCGCTCGACCAGATGAGCGTGTCGATGACCATGAACGGCGCGGTG
CTGCCGATCCTCGCTTTGTTCGTCGTCGCGGCGGAAGAGCAAGGCGTGCCGCCGGAGAAGCTGTCGGGCACCATTCAGAA
CGACATTCTGAAGGAGTTCATGGTGCGCAATACCTACATCTATCCGCCTGCGCCCTCGATGCGGATCATCTCGGACATCT
TCGCCTTCACCTCGCAGCGGATGCCGAAGTTCAATTCCATCTCGATCTCCGGCTATCACATGCAGGAGGCCGGCGCGACG
CAGGACCTCGAGCTCGCCTATACGCTCGCCGACGGCGTCGAGTACTTGCGCGCCGGCATCGCGGCGGGTCTCGACGTCGA
CCGCTTCGCGCCGAGGCTGTCGTTCTTCTGGGCGATCGGCATGAACTTCTTCATGGAGGTCGCCAAGCTGCGCGCCGCGC
GGCTGCTGTGGGCCAAGCTCCTGAAGCCGTTCAATCCGAAAGATCCGCGCTCGCTGTCCCTGCGCACGCACAGCCAGACC
TCGGGCTGGTCGCTGACGGCACAGGACGTCTACAACAACGTGGTGCGTACTGCGGTCGAGGCCATGGCGGCGACCCAAGG
CCACACGCAGTCGCTGCACACCAATGCGCTCGACGAGGCGCTGGCGCTGCCGACCGACTTCTCCGCGCGCATCGCCCGCA
ACACGCAATTGTTCCTGCAGCAGGAAAGCGGCACCACCCGCATCATCGATCCCTGGGGCGGCTCGTATTACGTCGAGCGC
CTCACCCGCGATCTCGCCGCCAAGGCCTGGGGCCACATCCAGGAGATCGAGGAACTCGGCGGAATGGCCAAGGCGATCGA
GGCCGGCGTGCCGAAGCTGCGCATCGAGGAGGCCTCGGCCAAGACGCAGGCGCGCATCGACACCGGCCGCCAGTCGGTGA
TCGGCGTCAACAAGTACAAGCCGACCGACGAGCCGCCGCTCGACGTGCTGAAGGTCGACAATTCCACCGTGCGCCGGCTG
CAGATCGACAAGCTCTCCAGGTTGAAATCCGAGCGCAAGCAGGCCGAGGTCGATGCGGCGCTCGCGGCGCTGACGCGCTC
GGCCGGCGACGGCAACGGCAACCTGCTGGCGCTGGCGATCGAGGCCGCGCGCGCGAAGGCGACCGTCGGCGAGATCTCCG
ATGCGCTGGAGAAGGTGTTCGGCCGCCATCGCGCCGAGATCAAGTCCATCACCGGCGTCTACAAGCGGGAGATCGCCGCC
ATGTCCGGCAAAGCCGAGAAGCTGCAGACCTTGATCGAGTCGTTCGAGGAAGCGGAAGGCCGAAGGCCGCGCATCCTCGT
CGCCAAGATCGGCCAGGACGGTCACGACCGCGGCCAGAAGGTGATCGCGTCAGCCTTCGCCGACATCGGCTTCGACGTCG
ACATCGGGCCGCTGTTCGCCACCGCCGACGAGGCCGCGCGCCAAGCGGTCGAGAACGACGTGCACATTCTCGGCGTCTCG
TCGCTCGCAGCCGCGCATCTCACCGCCGTGCCGGAGCTCAAGGCCGCGCTCAAGAAGCAGGGCCGCGAGGACATCATGAT
CATTGTCGGCGGCGTGGTGCCGCCGCAGGATTACGATGCGCTGTACAAATCCGGCGCCGAGGCGATCTTCCCGCCGGGCA
CCGTCATCGCGGATGCCGCCGAGGAGCTGATCCACAAGCTCAATGCGCGGCTGGGCCACAGCGAGGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
GGACAGACGTCGCGGCAGAGGATCGCATGAACGACCCGACCTATCAGAGCAACGTGCGGCACCTGATCGAGCGCAGCCGC
CAGTTTGAGGAAGCCCGGAA

Downstream 100 bases:

>100_bases
CGCGCCAGCCATTTACGCATCATTTGACGGCGCTCGGTCACGAGATCGCGGGCCGGTTGGTTTGAACCCGCGCCATCTCG
GGCGATGCGCATCCTCAGCC

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MTRIPNFADIAFEPTAAPAPAGAAEPWLTPEGIPVKPAYGEADLAGIDFLETWPGTAPYLRGPYPTMYVNQPWTVRQYAG
FSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVGGDVGMAGVAIDSIYDMRTLFAGIPLDQMSVSMTMNGAV
LPILALFVVAAEEQGVPPEKLSGTIQNDILKEFMVRNTYIYPPAPSMRIISDIFAFTSQRMPKFNSISISGYHMQEAGAT
QDLELAYTLADGVEYLRAGIAAGLDVDRFAPRLSFFWAIGMNFFMEVAKLRAARLLWAKLLKPFNPKDPRSLSLRTHSQT
SGWSLTAQDVYNNVVRTAVEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVER
LTRDLAAKAWGHIQEIEELGGMAKAIEAGVPKLRIEEASAKTQARIDTGRQSVIGVNKYKPTDEPPLDVLKVDNSTVRRL
QIDKLSRLKSERKQAEVDAALAALTRSAGDGNGNLLALAIEAARAKATVGEISDALEKVFGRHRAEIKSITGVYKREIAA
MSGKAEKLQTLIESFEEAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATADEAARQAVENDVHILGVS
SLAAAHLTAVPELKAALKKQGREDIMIIVGGVVPPQDYDALYKSGAEAIFPPGTVIADAAEELIHKLNARLGHSEAAE

Sequences:

>Translated_718_residues
MTRIPNFADIAFEPTAAPAPAGAAEPWLTPEGIPVKPAYGEADLAGIDFLETWPGTAPYLRGPYPTMYVNQPWTVRQYAG
FSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVGGDVGMAGVAIDSIYDMRTLFAGIPLDQMSVSMTMNGAV
LPILALFVVAAEEQGVPPEKLSGTIQNDILKEFMVRNTYIYPPAPSMRIISDIFAFTSQRMPKFNSISISGYHMQEAGAT
QDLELAYTLADGVEYLRAGIAAGLDVDRFAPRLSFFWAIGMNFFMEVAKLRAARLLWAKLLKPFNPKDPRSLSLRTHSQT
SGWSLTAQDVYNNVVRTAVEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVER
LTRDLAAKAWGHIQEIEELGGMAKAIEAGVPKLRIEEASAKTQARIDTGRQSVIGVNKYKPTDEPPLDVLKVDNSTVRRL
QIDKLSRLKSERKQAEVDAALAALTRSAGDGNGNLLALAIEAARAKATVGEISDALEKVFGRHRAEIKSITGVYKREIAA
MSGKAEKLQTLIESFEEAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATADEAARQAVENDVHILGVS
SLAAAHLTAVPELKAALKKQGREDIMIIVGGVVPPQDYDALYKSGAEAIFPPGTVIADAAEELIHKLNARLGHSEAAE
>Mature_717_residues
TRIPNFADIAFEPTAAPAPAGAAEPWLTPEGIPVKPAYGEADLAGIDFLETWPGTAPYLRGPYPTMYVNQPWTVRQYAGF
STAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVGGDVGMAGVAIDSIYDMRTLFAGIPLDQMSVSMTMNGAVL
PILALFVVAAEEQGVPPEKLSGTIQNDILKEFMVRNTYIYPPAPSMRIISDIFAFTSQRMPKFNSISISGYHMQEAGATQ
DLELAYTLADGVEYLRAGIAAGLDVDRFAPRLSFFWAIGMNFFMEVAKLRAARLLWAKLLKPFNPKDPRSLSLRTHSQTS
GWSLTAQDVYNNVVRTAVEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVERL
TRDLAAKAWGHIQEIEELGGMAKAIEAGVPKLRIEEASAKTQARIDTGRQSVIGVNKYKPTDEPPLDVLKVDNSTVRRLQ
IDKLSRLKSERKQAEVDAALAALTRSAGDGNGNLLALAIEAARAKATVGEISDALEKVFGRHRAEIKSITGVYKREIAAM
SGKAEKLQTLIESFEEAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATADEAARQAVENDVHILGVSS
LAAAHLTAVPELKAALKKQGREDIMIIVGGVVPPQDYDALYKSGAEAIFPPGTVIADAAEELIHKLNARLGHSEAAE

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthes

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=689, Percent_Identity=62.4092888243832, Blast_Score=902, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=683, Percent_Identity=61.9326500732064, Blast_Score=849, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=688, Percent_Identity=62.3546511627907, Blast_Score=893, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 77843; Mature: 77712

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIPNFADIAFEPTAAPAPAGAAEPWLTPEGIPVKPAYGEADLAGIDFLETWPGTAPYL
CCCCCCCHHCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
RGPYPTMYVNQPWTVRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPR
CCCCCEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCC
VGGDVGMAGVAIDSIYDMRTLFAGIPLDQMSVSMTMNGAVLPILALFVVAAEEQGVPPEK
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCHHHHHHHHHHHHHCCCCCCHHH
LSGTIQNDILKEFMVRNTYIYPPAPSMRIISDIFAFTSQRMPKFNSISISGYHMQEAGAT
HCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECEEECCCCCC
QDLELAYTLADGVEYLRAGIAAGLDVDRFAPRLSFFWAIGMNFFMEVAKLRAARLLWAKL
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKPFNPKDPRSLSLRTHSQTSGWSLTAQDVYNNVVRTAVEAMAATQGHTQSLHTNALDEA
HCCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVERLTRDLAAKAWGHIQEIEELG
HCCCCCCHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GMAKAIEAGVPKLRIEEASAKTQARIDTGRQSVIGVNKYKPTDEPPLDVLKVDNSTVRRL
HHHHHHHCCCCCEEEEHHCCHHHHHHHCCCHHEEECCCCCCCCCCCCCEEECCCHHHHHH
QIDKLSRLKSERKQAEVDAALAALTRSAGDGNGNLLALAIEAARAKATVGEISDALEKVF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHH
GRHRAEIKSITGVYKREIAAMSGKAEKLQTLIESFEEAEGRRPRILVAKIGQDGHDRGQK
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
VIASAFADIGFDVDIGPLFATADEAARQAVENDVHILGVSSLAAAHLTAVPELKAALKKQ
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHC
GREDIMIIVGGVVPPQDYDALYKSGAEAIFPPGTVIADAAEELIHKLNARLGHSEAAE
CCCEEEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TRIPNFADIAFEPTAAPAPAGAAEPWLTPEGIPVKPAYGEADLAGIDFLETWPGTAPYL
CCCCCCHHCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
RGPYPTMYVNQPWTVRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPR
CCCCCEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCC
VGGDVGMAGVAIDSIYDMRTLFAGIPLDQMSVSMTMNGAVLPILALFVVAAEEQGVPPEK
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCHHHHHHHHHHHHHCCCCCCHHH
LSGTIQNDILKEFMVRNTYIYPPAPSMRIISDIFAFTSQRMPKFNSISISGYHMQEAGAT
HCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECEEECCCCCC
QDLELAYTLADGVEYLRAGIAAGLDVDRFAPRLSFFWAIGMNFFMEVAKLRAARLLWAKL
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKPFNPKDPRSLSLRTHSQTSGWSLTAQDVYNNVVRTAVEAMAATQGHTQSLHTNALDEA
HCCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVERLTRDLAAKAWGHIQEIEELG
HCCCCCCHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GMAKAIEAGVPKLRIEEASAKTQARIDTGRQSVIGVNKYKPTDEPPLDVLKVDNSTVRRL
HHHHHHHCCCCCEEEEHHCCHHHHHHHCCCHHEEECCCCCCCCCCCCCEEECCCHHHHHH
QIDKLSRLKSERKQAEVDAALAALTRSAGDGNGNLLALAIEAARAKATVGEISDALEKVF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHH
GRHRAEIKSITGVYKREIAAMSGKAEKLQTLIESFEEAEGRRPRILVAKIGQDGHDRGQK
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
VIASAFADIGFDVDIGPLFATADEAARQAVENDVHILGVSSLAAAHLTAVPELKAALKKQ
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHC
GREDIMIIVGGVVPPQDYDALYKSGAEAIFPPGTVIADAAEELIHKLNARLGHSEAAE
CCCEEEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099072 [H]