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The map label for this gene is yngF [H]

Identifier: 146339474

GI number: 146339474

Start: 2623897

End: 2624709

Strand: Reverse

Name: yngF [H]

Synonym: BRADO2462

Alternate gene names: 146339474

Gene position: 2624709-2623897 (Counterclockwise)

Preceding gene: 146339475

Following gene: 146339473

Centisome position: 35.2

GC content: 68.14

Gene sequence:

>813_bases
ATGTCACCTGCCTCTCCTACCGCCTTTGCCGAGGGCAAGATCCTCCTGACCGTCGGCGACGGGGTCGGGATCGTCACCTT
CAACAATCCCGAGAAGCGCAACGCGATGTCGTTGCAGATGTGGGAGGGCCTCGGCCACGCCCTGGTCGCGCTGCGCGACG
ACGCCGCCGTGCGGGTCGTGGTGCTGACCGGCGCCGGCGACAAGGCGTTCGTGTCGGGCGCCGATATCAGCCAGTTCGAG
CAGAGCCGCCACAATGCGGAGGCCTCCGAGGCCTATGCGCAGCGGAGCGCCACCCAGCGGGCGCTGCTCGCCGACTTCCC
CAAGCCGACGATCGCCTGCATCCGCGGCTTCTGCCTCGGCGGCGGGCTCCAGATTGCGATGCTGGCCGACATGCGCCTCG
CGGCTACCGACAGCCAGTTCGGCATTCCCGCGGCCAGGCTCGGCATCGCCTACGGCCATGATGGCCTGCGCAACCTGGTG
TCGCTGGTCGGCCCGTCGCAGGCGCGGCTGCTGCTCTATACCGGCCAGCGCATCGACGCCACGGAGGCCCTGCGGATCGG
CCTCATCGATCGGATGATGAACGTTGACGAACTGCTGCCGGCCACGCTCGACATCGCCCGCACCATCGCCGGCAACGCGC
CGCTCGCGGTACGGGCCGCCAAGATCACCATCGCCGAAGTGCTAAAGGACGAGAGCCGACGCGACATGGCCGCGATCAAG
CGGATCGGCGACGCCTGCATGGATTCCGAGGATTTCCGCGAGGGCCGCCGCGCCTTCATGGAAAAGCGCAAGCCGGTTTT
CAAAGGGCGCTGA

Upstream 100 bases:

>100_bases
TGATCCTGGCGCGGCTCTCAAACCTGATCTTGCGTTGACGCCGCATGGGTCGCGGTGAACGATGGCGTCAACCTGCCGCC
TGCTCCGGATGCCCATGCCG

Downstream 100 bases:

>100_bases
ACGCGGCCGCCATCTCTCTCGACTGACACGGTCGACGCGCATCTTTTTGCGGCGCGCGTGCCCGCAAAATCGTCTTGCGG
CTGCGTGAACGCTGATGTCA

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSPASPTAFAEGKILLTVGDGVGIVTFNNPEKRNAMSLQMWEGLGHALVALRDDAAVRVVVLTGAGDKAFVSGADISQFE
QSRHNAEASEAYAQRSATQRALLADFPKPTIACIRGFCLGGGLQIAMLADMRLAATDSQFGIPAARLGIAYGHDGLRNLV
SLVGPSQARLLLYTGQRIDATEALRIGLIDRMMNVDELLPATLDIARTIAGNAPLAVRAAKITIAEVLKDESRRDMAAIK
RIGDACMDSEDFREGRRAFMEKRKPVFKGR

Sequences:

>Translated_270_residues
MSPASPTAFAEGKILLTVGDGVGIVTFNNPEKRNAMSLQMWEGLGHALVALRDDAAVRVVVLTGAGDKAFVSGADISQFE
QSRHNAEASEAYAQRSATQRALLADFPKPTIACIRGFCLGGGLQIAMLADMRLAATDSQFGIPAARLGIAYGHDGLRNLV
SLVGPSQARLLLYTGQRIDATEALRIGLIDRMMNVDELLPATLDIARTIAGNAPLAVRAAKITIAEVLKDESRRDMAAIK
RIGDACMDSEDFREGRRAFMEKRKPVFKGR
>Mature_269_residues
SPASPTAFAEGKILLTVGDGVGIVTFNNPEKRNAMSLQMWEGLGHALVALRDDAAVRVVVLTGAGDKAFVSGADISQFEQ
SRHNAEASEAYAQRSATQRALLADFPKPTIACIRGFCLGGGLQIAMLADMRLAATDSQFGIPAARLGIAYGHDGLRNLVS
LVGPSQARLLLYTGQRIDATEALRIGLIDRMMNVDELLPATLDIARTIAGNAPLAVRAAKITIAEVLKDESRRDMAAIKR
IGDACMDSEDFREGRRAFMEKRKPVFKGR

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=32.1285140562249, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=28.968253968254, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI4502327, Length=259, Percent_Identity=30.5019305019305, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI70995211, Length=211, Percent_Identity=31.2796208530806, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI45643119, Length=235, Percent_Identity=25.9574468085106, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI260275230, Length=235, Percent_Identity=25.9574468085106, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI260274832, Length=235, Percent_Identity=25.9574468085106, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI68989263, Length=186, Percent_Identity=30.1075268817204, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=29.7752808988764, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI213417737, Length=211, Percent_Identity=25.5924170616114, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI157694516, Length=211, Percent_Identity=25.5924170616114, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594469, Length=227, Percent_Identity=25.1101321585903, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI37594471, Length=227, Percent_Identity=25.1101321585903, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI221142681, Length=251, Percent_Identity=32.2709163346614, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=32.1285140562249, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=31.7647058823529, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788597, Length=259, Percent_Identity=30.8880308880309, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788682, Length=203, Percent_Identity=31.0344827586207, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790281, Length=218, Percent_Identity=28.8990825688073, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=27.8195488721804, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=29.3172690763052, Blast_Score=120, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=29.8387096774194, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17540714, Length=213, Percent_Identity=29.5774647887324, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=29.3233082706767, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=217, Percent_Identity=30.8755760368664, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=243, Percent_Identity=24.6913580246914, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=164, Percent_Identity=31.7073170731707, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17508951, Length=248, Percent_Identity=27.8225806451613, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=248, Percent_Identity=27.8225806451613, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=248, Percent_Identity=27.8225806451613, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=200, Percent_Identity=27.5, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=200, Percent_Identity=27.5, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=244, Percent_Identity=27.4590163934426, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17549921, Length=182, Percent_Identity=28.021978021978, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=31.1284046692607, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI20129971, Length=255, Percent_Identity=30.9803921568627, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653477, Length=255, Percent_Identity=30.9803921568627, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19920382, Length=256, Percent_Identity=28.515625, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24650670, Length=206, Percent_Identity=28.6407766990291, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24654903, Length=178, Percent_Identity=28.6516853932584, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=24.6153846153846, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21357171, Length=197, Percent_Identity=25.8883248730964, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583077, Length=268, Percent_Identity=27.9850746268657, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583079, Length=268, Percent_Identity=27.9850746268657, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921000, Length=199, Percent_Identity=29.1457286432161, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571729, Length=197, Percent_Identity=25.8883248730964, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28571730, Length=197, Percent_Identity=25.8883248730964, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28997; Mature: 28866

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPASPTAFAEGKILLTVGDGVGIVTFNNPEKRNAMSLQMWEGLGHALVALRDDAAVRVV
CCCCCCCEECCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEEECCCEEEEE
VLTGAGDKAFVSGADISQFEQSRHNAEASEAYAQRSATQRALLADFPKPTIACIRGFCLG
EEECCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
GGLQIAMLADMRLAATDSQFGIPAARLGIAYGHDGLRNLVSLVGPSQARLLLYTGQRIDA
CCEEEEEEECCCEEECCCCCCCCHHHHEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCH
TEALRIGLIDRMMNVDELLPATLDIARTIAGNAPLAVRAAKITIAEVLKDESRRDMAAIK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHH
RIGDACMDSEDFREGRRAFMEKRKPVFKGR
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SPASPTAFAEGKILLTVGDGVGIVTFNNPEKRNAMSLQMWEGLGHALVALRDDAAVRVV
CCCCCCEECCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEEECCCEEEEE
VLTGAGDKAFVSGADISQFEQSRHNAEASEAYAQRSATQRALLADFPKPTIACIRGFCLG
EEECCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
GGLQIAMLADMRLAATDSQFGIPAARLGIAYGHDGLRNLVSLVGPSQARLLLYTGQRIDA
CCEEEEEEECCCEEECCCCCCCCHHHHEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCH
TEALRIGLIDRMMNVDELLPATLDIARTIAGNAPLAVRAAKITIAEVLKDESRRDMAAIK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHH
RIGDACMDSEDFREGRRAFMEKRKPVFKGR
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]