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The map label for this gene is cysB [H]

Identifier: 146339463

GI number: 146339463

Start: 2609487

End: 2610377

Strand: Reverse

Name: cysB [H]

Synonym: BRADO2449

Alternate gene names: 146339463

Gene position: 2610377-2609487 (Counterclockwise)

Preceding gene: 146339467

Following gene: 146339461

Centisome position: 35.01

GC content: 68.13

Gene sequence:

>891_bases
ATGCATCAGGTCCGGTATTTTCTTGCCGTCGCCCGGCTGCTGCACTTCACGCGCGCGGCCGAGGAATGCAACGTCACGCA
GCCCTCGCTGACCCGCGCCATCAAGCAGCTCGAGGCCGAGCTCGGCGGCGACCTGTTCAGGCGCGAGCGGCCGGCCGCAC
AGCTGACCGAGCTCGGCGAGCGCATGTATCCGCTGCTGAAGCAGTGCTACGAGGCCGCCGCCGGCGCACGTACCCTTGCT
TCGTCCTTCAAGAGTGGCGAGGTCGGCACGCTCAGGATCGCGCTCGCCAATTCGGTCGACCTCGCCGTGCTGCTTCCGAT
GCTCGAGCAGTTGCAGCGGCAGTTCAACAAGCTCGAGTTCAGATTCCTGCGCGGCAGCAGCCGCGCGGTTGCGGACTATC
TCAAGAGCGGCGACGCCGAACTCGGCATCGCCGCCGAGATCGGCGAGATCTGGGATCGGCTCGATGTGTGGCCGCTGTTC
ACCGAGGATTTCGACGTCGCGGTGAGCGACAGGCATCGGCTCGCCGCGCAGGACACGATCGCCGTCGCGGAACTCGGCCG
CGATGCGCTGCTGTCGCGGGGCTATTGCGAGCATGGCGAGCGGCTGCGAACGCAGCTGCGGGAGCTCGGCGTCGAGATCG
AGCGCAGCCATGACGTCCATTCCGAGCGCGACCTGATCGCATTGGTCGAGGCCGGGGTCGGCATCGGCGTGGTGCCGCAG
ACCACCAGACTGCCGGCGACGCTGCGGCGCACGGCGCTGGATGGACTCGACGCGCGGCGGACGATCCACCTTTATGGCGT
CGCCGGCCGCGAGCGGACCGCCGTCGCATCCGCGGTCATGCGCATGTTGCGCGGCAGGGACTGGCAGAAATGGCTGGGTC
AGCTGGCGTGA

Upstream 100 bases:

>100_bases
ATAGGATGCCTCTATCGCCATCGGGTGATGCCAGTTGGTAGTCCCCAGTCAGATTGAAGCCGGGCGATCTGGGCCCGGTG
GAACGGAGCGATCCATGGAA

Downstream 100 bases:

>100_bases
GCGCGCTCACCGGCATGATCAGGCGGGGGCGGCCTGCGCGAGCGCGGCAACGAGATCGTCGATCTCCATCCGGCGACGGA
AATCAGGATCGATGTAGCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MHQVRYFLAVARLLHFTRAAEECNVTQPSLTRAIKQLEAELGGDLFRRERPAAQLTELGERMYPLLKQCYEAAAGARTLA
SSFKSGEVGTLRIALANSVDLAVLLPMLEQLQRQFNKLEFRFLRGSSRAVADYLKSGDAELGIAAEIGEIWDRLDVWPLF
TEDFDVAVSDRHRLAAQDTIAVAELGRDALLSRGYCEHGERLRTQLRELGVEIERSHDVHSERDLIALVEAGVGIGVVPQ
TTRLPATLRRTALDGLDARRTIHLYGVAGRERTAVASAVMRMLRGRDWQKWLGQLA

Sequences:

>Translated_296_residues
MHQVRYFLAVARLLHFTRAAEECNVTQPSLTRAIKQLEAELGGDLFRRERPAAQLTELGERMYPLLKQCYEAAAGARTLA
SSFKSGEVGTLRIALANSVDLAVLLPMLEQLQRQFNKLEFRFLRGSSRAVADYLKSGDAELGIAAEIGEIWDRLDVWPLF
TEDFDVAVSDRHRLAAQDTIAVAELGRDALLSRGYCEHGERLRTQLRELGVEIERSHDVHSERDLIALVEAGVGIGVVPQ
TTRLPATLRRTALDGLDARRTIHLYGVAGRERTAVASAVMRMLRGRDWQKWLGQLA
>Mature_296_residues
MHQVRYFLAVARLLHFTRAAEECNVTQPSLTRAIKQLEAELGGDLFRRERPAAQLTELGERMYPLLKQCYEAAAGARTLA
SSFKSGEVGTLRIALANSVDLAVLLPMLEQLQRQFNKLEFRFLRGSSRAVADYLKSGDAELGIAAEIGEIWDRLDVWPLF
TEDFDVAVSDRHRLAAQDTIAVAELGRDALLSRGYCEHGERLRTQLRELGVEIERSHDVHSERDLIALVEAGVGIGVVPQ
TTRLPATLRRTALDGLDARRTIHLYGVAGRERTAVASAVMRMLRGRDWQKWLGQLA

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790399, Length=270, Percent_Identity=28.8888888888889, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI157672245, Length=192, Percent_Identity=30.7291666666667, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787530, Length=242, Percent_Identity=23.1404958677686, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787879, Length=258, Percent_Identity=25.1937984496124, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693105, Length=245, Percent_Identity=25.3061224489796, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367136, Length=237, Percent_Identity=24.0506329113924, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=22.2222222222222, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33100; Mature: 33100

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQVRYFLAVARLLHFTRAAEECNVTQPSLTRAIKQLEAELGGDLFRRERPAAQLTELGE
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHH
RMYPLLKQCYEAAAGARTLASSFKSGEVGTLRIALANSVDLAVLLPMLEQLQRQFNKLEF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
RFLRGSSRAVADYLKSGDAELGIAAEIGEIWDRLDVWPLFTEDFDVAVSDRHRLAAQDTI
HHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHH
AVAELGRDALLSRGYCEHGERLRTQLRELGVEIERSHDVHSERDLIALVEAGVGIGVVPQ
HHHHHCHHHHHHCCHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCC
TTRLPATLRRTALDGLDARRTIHLYGVAGRERTAVASAVMRMLRGRDWQKWLGQLA
CCCCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure
MHQVRYFLAVARLLHFTRAAEECNVTQPSLTRAIKQLEAELGGDLFRRERPAAQLTELGE
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHH
RMYPLLKQCYEAAAGARTLASSFKSGEVGTLRIALANSVDLAVLLPMLEQLQRQFNKLEF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
RFLRGSSRAVADYLKSGDAELGIAAEIGEIWDRLDVWPLFTEDFDVAVSDRHRLAAQDTI
HHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHH
AVAELGRDALLSRGYCEHGERLRTQLRELGVEIERSHDVHSERDLIALVEAGVGIGVVPQ
HHHHHCHHHHHHCCHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCC
TTRLPATLRRTALDGLDARRTIHLYGVAGRERTAVASAVMRMLRGRDWQKWLGQLA
CCCCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]