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The map label for this gene is fadH [C]

Identifier: 146339368

GI number: 146339368

Start: 2501567

End: 2502685

Strand: Direct

Name: fadH [C]

Synonym: BRADO2349

Alternate gene names: 146339368

Gene position: 2501567-2502685 (Clockwise)

Preceding gene: 146339366

Following gene: 146339370

Centisome position: 33.55

GC content: 68.28

Gene sequence:

>1119_bases
ATGTCCGATCTCGATACCAGCGTGCTGTTTCGTCCGTTTCGCCTGAAATCGCTGGAGCTGAAGAACCGCATCGTCATGGC
GCCGATGACGCGCAACGCCGCGCCGAACGGCGTTCCCGGCGAAGCCAACGCCGCCTATTACCGCCGCCGCGCCGAAGGCG
GCGTGGGGCTGATCCTGACCGAGGGCACGGTGATCAACCGTCCGGCCTCGCGCAACGAGCCTAACATTCCCTTCTTCCAC
GGCGAGGCCGCGCTGGCCGGCTGGCAGAGCGTGGCGAGCGCCGTGCACGCCGCCGGCGGCCGCATCGCGCCGCAGATCTG
GCACACCGGCTCCACCAAGGGCATAAGCCGCTGGGAGCCGGACGCGCCGGTCGAGAGCCCGTCGGGCCTGGTCGCGCCGG
GCAAGCCACGCGGCAACATCATGACCGAGACCGACATCGCCGACACGGTTGCCGCCTTCGCGCAGGCTGCGGCCGATGCC
AAGCGCACCGGCTTCGACACGGTCGAAATCCACGGCGCCCATGGCTATCTTGTCGATGAGTTCTTCTGGTCCGGCACCAA
CAAGCGCGAGGACCGCTATGGTGGCCCGACCATCAAGGAGCGCTCGCGGTTTGCCGGCGAGATCGTCTCCGCGATCCGCA
AGGCTGTTGGTCCCGACTTCCCGATCATCCTGCGCGTCAGCCAGTGGAAGCAGCAGGACTACGCGACGCGCCTGGCCGAG
ACGCCGGCGCTGATGGGCGACTGGCTGCAGCCGCTGGTCGACGCCGGCGTCGACATCCTGCACTGCTCGCAGCGCCGCTT
CTGGGAACCGGAATTCCCGAAGATCGACGGCGAGAAGGGGCTCAACTTCGCCGGCTGGGCCAAGAAGATCACGGGCGCCG
CGACCATCAGTGTCGGCTCGGTCGGGCTGTCCGACGAGTTCTTCTCGGCGTTCGCCGGCAAGCCGTCGACCTCGGCCGAT
CTCGACGCGCTGCTCGAGCGGATGGCGAAGGACGAGTTCGACCTCATCGCCGTCGGCCGCGCGCTGATCAGCGATGCCGA
CTGGGTCGCCAAGATCAAGGCCGGCGACAAGGCCGGGCTGAAGGGCTTCAACGCCGCGGACCTCGGCGCGCTGGTGTGA

Upstream 100 bases:

>100_bases
GGTGGTTTCCTTTGGGATACCTGAGCGACTTCGGGTGCCCCATTGACTTCAGGTTTACGAAGTATACTGGAGCGGTTCCG
AACGCAAGGGAGCCTTCTGA

Downstream 100 bases:

>100_bases
GTTGGGTCGCGGCTTCTTCACCAAGTCCGTTCGCGTCCTCCTGCTAGAGCGTGGGTCGCCTGCGTTGTTAGTTGGATGCG
AATAAAAACCACGGAGTCGG

Product: putative oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSDLDTSVLFRPFRLKSLELKNRIVMAPMTRNAAPNGVPGEANAAYYRRRAEGGVGLILTEGTVINRPASRNEPNIPFFH
GEAALAGWQSVASAVHAAGGRIAPQIWHTGSTKGISRWEPDAPVESPSGLVAPGKPRGNIMTETDIADTVAAFAQAAADA
KRTGFDTVEIHGAHGYLVDEFFWSGTNKREDRYGGPTIKERSRFAGEIVSAIRKAVGPDFPIILRVSQWKQQDYATRLAE
TPALMGDWLQPLVDAGVDILHCSQRRFWEPEFPKIDGEKGLNFAGWAKKITGAATISVGSVGLSDEFFSAFAGKPSTSAD
LDALLERMAKDEFDLIAVGRALISDADWVAKIKAGDKAGLKGFNAADLGALV

Sequences:

>Translated_372_residues
MSDLDTSVLFRPFRLKSLELKNRIVMAPMTRNAAPNGVPGEANAAYYRRRAEGGVGLILTEGTVINRPASRNEPNIPFFH
GEAALAGWQSVASAVHAAGGRIAPQIWHTGSTKGISRWEPDAPVESPSGLVAPGKPRGNIMTETDIADTVAAFAQAAADA
KRTGFDTVEIHGAHGYLVDEFFWSGTNKREDRYGGPTIKERSRFAGEIVSAIRKAVGPDFPIILRVSQWKQQDYATRLAE
TPALMGDWLQPLVDAGVDILHCSQRRFWEPEFPKIDGEKGLNFAGWAKKITGAATISVGSVGLSDEFFSAFAGKPSTSAD
LDALLERMAKDEFDLIAVGRALISDADWVAKIKAGDKAGLKGFNAADLGALV
>Mature_371_residues
SDLDTSVLFRPFRLKSLELKNRIVMAPMTRNAAPNGVPGEANAAYYRRRAEGGVGLILTEGTVINRPASRNEPNIPFFHG
EAALAGWQSVASAVHAAGGRIAPQIWHTGSTKGISRWEPDAPVESPSGLVAPGKPRGNIMTETDIADTVAAFAQAAADAK
RTGFDTVEIHGAHGYLVDEFFWSGTNKREDRYGGPTIKERSRFAGEIVSAIRKAVGPDFPIILRVSQWKQQDYATRLAET
PALMGDWLQPLVDAGVDILHCSQRRFWEPEFPKIDGEKGLNFAGWAKKITGAATISVGSVGLSDEFFSAFAGKPSTSADL
DALLERMAKDEFDLIAVGRALISDADWVAKIKAGDKAGLKGFNAADLGALV

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=351, Percent_Identity=28.7749287749288, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787939, Length=245, Percent_Identity=35.5102040816327, Blast_Score=121, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17565138, Length=230, Percent_Identity=30, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI72001454, Length=214, Percent_Identity=31.3084112149533, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559802, Length=262, Percent_Identity=28.2442748091603, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17564188, Length=219, Percent_Identity=30.5936073059361, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17559804, Length=385, Percent_Identity=25.7142857142857, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17540738, Length=256, Percent_Identity=28.90625, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=374, Percent_Identity=28.0748663101604, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321973, Length=376, Percent_Identity=28.1914893617021, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 40005; Mature: 39873

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLDTSVLFRPFRLKSLELKNRIVMAPMTRNAAPNGVPGEANAAYYRRRAEGGVGLILT
CCCCCCHHHCCCHHHCCEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEE
EGTVINRPASRNEPNIPFFHGEAALAGWQSVASAVHAAGGRIAPQIWHTGSTKGISRWEP
CCEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCHHEEECCCCCCCCCCCC
DAPVESPSGLVAPGKPRGNIMTETDIADTVAAFAQAAADAKRTGFDTVEIHGAHGYLVDE
CCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
FFWSGTNKREDRYGGPTIKERSRFAGEIVSAIRKAVGPDFPIILRVSQWKQQDYATRLAE
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHH
TPALMGDWLQPLVDAGVDILHCSQRRFWEPEFPKIDGEKGLNFAGWAKKITGAATISVGS
CHHHHHHHHHHHHHCCCCEEECCHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECC
VGLSDEFFSAFAGKPSTSADLDALLERMAKDEFDLIAVGRALISDADWVAKIKAGDKAGL
CCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCC
KGFNAADLGALV
CCCCCHHHHCCC
>Mature Secondary Structure 
SDLDTSVLFRPFRLKSLELKNRIVMAPMTRNAAPNGVPGEANAAYYRRRAEGGVGLILT
CCCCCHHHCCCHHHCCEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEE
EGTVINRPASRNEPNIPFFHGEAALAGWQSVASAVHAAGGRIAPQIWHTGSTKGISRWEP
CCEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCHHEEECCCCCCCCCCCC
DAPVESPSGLVAPGKPRGNIMTETDIADTVAAFAQAAADAKRTGFDTVEIHGAHGYLVDE
CCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
FFWSGTNKREDRYGGPTIKERSRFAGEIVSAIRKAVGPDFPIILRVSQWKQQDYATRLAE
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHH
TPALMGDWLQPLVDAGVDILHCSQRRFWEPEFPKIDGEKGLNFAGWAKKITGAATISVGS
CHHHHHHHHHHHHHCCCCEEECCHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECC
VGLSDEFFSAFAGKPSTSADLDALLERMAKDEFDLIAVGRALISDADWVAKIKAGDKAGL
CCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCC
KGFNAADLGALV
CCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]