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The map label for this gene is livK [C]

Identifier: 146339363

GI number: 146339363

Start: 2496401

End: 2497522

Strand: Reverse

Name: livK [C]

Synonym: BRADO2344

Alternate gene names: 146339363

Gene position: 2497522-2496401 (Counterclockwise)

Preceding gene: 146339364

Following gene: 146339352

Centisome position: 33.49

GC content: 64.08

Gene sequence:

>1122_bases
TTGGCCGCCACGCTCTGGGCAGGCGCGGCGCAAGCCGAAAACACGGTCTACATTCCCGACGTCGTCGAGCTGTCCGGCCC
CGGCGCGGTGTCGGGCACCAATTGGCGCGACGGTGTCGCGCTGGCGGTCGACGAGATCAACGCCGCCGGCGGCATTCTCG
GCCGGAAGATCGTGACCGAACATCTGGATACGCAGAGCAATCCGGGCGTGTCGCGCGCCCAGGTGCAGAAGGTGCTCGAT
CGCGAGCCGTACGTGGTGCTGGGGCCGATCTATTCCGGCTCGGTCAAGGTCAACATGGCGCTGACGCAACAGGCGGAGGT
GCCGCAGATCGTCGGCGCCGAGGCCGCCGATATCACCACGCAGGGCAATCCCTGGATCTTCCGCACCGCCTTCGGCCAGC
AGTTCAGCATGCCGAAGATCGCCAACTATCTGCACGACCAGCTCAAGGTGAAGTCGGTCGCGGTCGTCTGGGTCAACAAC
GATTTCGGCAAGGGCGGCCGCGACAGCTTCGTCAAGGAGATGAAGGTCCGCGGCATCGAGATCGCGTCCGACCTCTCAAC
CGAGCAGGGCCAGGTCGATTTCGGCTCCGACGTCATCAAGCTGAAGAACGTCAAGGCCGATGCCGTCTTCGTCTACACCA
ACGAGGAGGAGAGCGCGCGCTTCCTGATCGAGGCCAAGCGTCAGGGATTGACGGTTCCGCTGTTCGGCGAGACCACGCTC
CTGAGCCAGAAGGTCGTCGAGCTCGCGGGCCCCGCCGCCAATGGCGTACGCGGCCACGTCGGCCTGAGCGCGGATGCGCC
CGTTCCGGCCATTCAGGCATTTGCGAAGAAGTTCAGCGATCGCTATCGCTATTGGCCGGATCACAACGGCATCAAGGGCT
ATACCGCGGTCTATCTCGTCAAATACGTGACCGAGATCATCGGCAAGTTCGACGGCCACGCCTTCGGCACGACCATGAAG
GGCCTGACGCTGACGCCCGACAAGGCGCCGGGCATGCTGATGGAAGCGAGCTGGGATCAGAACGGCGACATCGACCGCGA
GAGCTTCCTGGCGGAGATCGTCGACGGCAAGCAGAAGATCGTGGCGACCTTGCCGAAGCTGAAGCCGTCCAAGGCGCCGT
AA

Upstream 100 bases:

>100_bases
TTGCTCGGCTTTGCGGAGGCGCCGCCGGATCGATCGACATAAGCAAAAAAGAAATGGGGAGGACATCATGAGGCATTTGA
AATTATTGAGAGGGGTGGCG

Downstream 100 bases:

>100_bases
CATCGGCGCAGCCCGGCTTTCGGCCCGCAGAAGTCCTCCCCGCGCACACCGCGCGCGAGGAGGAGTTGATATCAACCCTT
GGCGAGCTGCTCGTCGAGGT

Product: branched chain amino-acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MAATLWAGAAQAENTVYIPDVVELSGPGAVSGTNWRDGVALAVDEINAAGGILGRKIVTEHLDTQSNPGVSRAQVQKVLD
REPYVVLGPIYSGSVKVNMALTQQAEVPQIVGAEAADITTQGNPWIFRTAFGQQFSMPKIANYLHDQLKVKSVAVVWVNN
DFGKGGRDSFVKEMKVRGIEIASDLSTEQGQVDFGSDVIKLKNVKADAVFVYTNEEESARFLIEAKRQGLTVPLFGETTL
LSQKVVELAGPAANGVRGHVGLSADAPVPAIQAFAKKFSDRYRYWPDHNGIKGYTAVYLVKYVTEIIGKFDGHAFGTTMK
GLTLTPDKAPGMLMEASWDQNGDIDRESFLAEIVDGKQKIVATLPKLKPSKAP

Sequences:

>Translated_373_residues
MAATLWAGAAQAENTVYIPDVVELSGPGAVSGTNWRDGVALAVDEINAAGGILGRKIVTEHLDTQSNPGVSRAQVQKVLD
REPYVVLGPIYSGSVKVNMALTQQAEVPQIVGAEAADITTQGNPWIFRTAFGQQFSMPKIANYLHDQLKVKSVAVVWVNN
DFGKGGRDSFVKEMKVRGIEIASDLSTEQGQVDFGSDVIKLKNVKADAVFVYTNEEESARFLIEAKRQGLTVPLFGETTL
LSQKVVELAGPAANGVRGHVGLSADAPVPAIQAFAKKFSDRYRYWPDHNGIKGYTAVYLVKYVTEIIGKFDGHAFGTTMK
GLTLTPDKAPGMLMEASWDQNGDIDRESFLAEIVDGKQKIVATLPKLKPSKAP
>Mature_372_residues
AATLWAGAAQAENTVYIPDVVELSGPGAVSGTNWRDGVALAVDEINAAGGILGRKIVTEHLDTQSNPGVSRAQVQKVLDR
EPYVVLGPIYSGSVKVNMALTQQAEVPQIVGAEAADITTQGNPWIFRTAFGQQFSMPKIANYLHDQLKVKSVAVVWVNND
FGKGGRDSFVKEMKVRGIEIASDLSTEQGQVDFGSDVIKLKNVKADAVFVYTNEEESARFLIEAKRQGLTVPLFGETTLL
SQKVVELAGPAANGVRGHVGLSADAPVPAIQAFAKKFSDRYRYWPDHNGIKGYTAVYLVKYVTEIIGKFDGHAFGTTMKG
LTLTPDKAPGMLMEASWDQNGDIDRESFLAEIVDGKQKIVATLPKLKPSKAP

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=234, Percent_Identity=25.6410256410256, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994941, Length=234, Percent_Identity=25.6410256410256, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40250; Mature: 40119

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATLWAGAAQAENTVYIPDVVELSGPGAVSGTNWRDGVALAVDEINAAGGILGRKIVTE
CCCEEECCCCCCCCEEECCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
HLDTQSNPGVSRAQVQKVLDREPYVVLGPIYSGSVKVNMALTQQAEVPQIVGAEAADITT
HCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEEECCCCCCHHHCCCCCCEEE
QGNPWIFRTAFGQQFSMPKIANYLHDQLKVKSVAVVWVNNDFGKGGRDSFVKEMKVRGIE
CCCCEEEEECCCCCCCCHHHHHHHHHHHEEEEEEEEEEECCCCCCCHHHHHHHHHHCCEE
IASDLSTEQGQVDFGSDVIKLKNVKADAVFVYTNEEESARFLIEAKRQGLTVPLFGETTL
EHHCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHEEHHHCCCEEEECCCHHH
LSQKVVELAGPAANGVRGHVGLSADAPVPAIQAFAKKFSDRYRYWPDHNGIKGYTAVYLV
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
KYVTEIIGKFDGHAFGTTMKGLTLTPDKAPGMLMEASWDQNGDIDRESFLAEIVDGKQKI
HHHHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHH
VATLPKLKPSKAP
HHHCCCCCCCCCC
>Mature Secondary Structure 
AATLWAGAAQAENTVYIPDVVELSGPGAVSGTNWRDGVALAVDEINAAGGILGRKIVTE
CCEEECCCCCCCCEEECCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
HLDTQSNPGVSRAQVQKVLDREPYVVLGPIYSGSVKVNMALTQQAEVPQIVGAEAADITT
HCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEEECCCCCCHHHCCCCCCEEE
QGNPWIFRTAFGQQFSMPKIANYLHDQLKVKSVAVVWVNNDFGKGGRDSFVKEMKVRGIE
CCCCEEEEECCCCCCCCHHHHHHHHHHHEEEEEEEEEEECCCCCCCHHHHHHHHHHCCEE
IASDLSTEQGQVDFGSDVIKLKNVKADAVFVYTNEEESARFLIEAKRQGLTVPLFGETTL
EHHCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHEEHHHCCCEEEECCCHHH
LSQKVVELAGPAANGVRGHVGLSADAPVPAIQAFAKKFSDRYRYWPDHNGIKGYTAVYLV
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
KYVTEIIGKFDGHAFGTTMKGLTLTPDKAPGMLMEASWDQNGDIDRESFLAEIVDGKQKI
HHHHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHH
VATLPKLKPSKAP
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA