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The map label for this gene is bclA [H]

Identifier: 146339346

GI number: 146339346

Start: 2480214

End: 2481887

Strand: Direct

Name: bclA [H]

Synonym: BRADO2327

Alternate gene names: 146339346

Gene position: 2480214-2481887 (Clockwise)

Preceding gene: 146339345

Following gene: 146339347

Centisome position: 33.26

GC content: 66.43

Gene sequence:

>1674_bases
ATGGCCACTGCCGCCAAGGTTCAGCAGCCGGAGAGCGCGCACAGCGCCCACGTCGACACCTTCGCCCAGGACAATCTGCC
GCCGCGCGAGATGTGGCCGGATTTCGTGTTCAGCCGCCCCGAACTCCAGTACCCGCCGCGGCTGAACTGCGTGACGCATT
TCCTCGACCGCTGGGTGGAAGAGGGACGCGGCGACGCGCCCTGCATCATCAGCACGGAGGTCAGCTACACCTATCGTGAG
CTGCAGGCGCTGGTGAACAAGATCGCCAACGTCCTCGTCGGCAAGCTCGGCCTGGTGCCCGGCGGCCGCGTGCTGCTGCG
GTCCGCCAACAATCCGATGATGGTCGCGACTTATCTCGCCGCGATCAAGGCCGGTGGCATCGTGGTCGCGACCATGCCGC
TGTTGCGCGCGAAGGAGTTGTCCTATCCGATTCAGAAGGCGCAGATCGCGCTGGCGCTGTGCGACGGCAAGCTCGCCGAC
GAGATGGAGAAGACCCGCGCGGTCGCGCCGGCGCTGAAGCAGGTCGTGTATTGGGGCAACGGGCAGCCCGACGCTCTGGA
AGCGCTGATCGCGGATGCAAGCCCCGACTTCGCCGCCCTCGACACGGCGTCCGACGATCTGTGCCTGATCGCCTTCACTT
CCGGCACGACCGGCGATCCCAAGGGCACCATGCATTTCCACCGCGACATGCTCGCGGTGTGCGACGGCTATGCCCGTAAC
GTGCTGCGCGCCAGCCAGAGCGACCGATTCATCGGCACCGCGCCGCTCGCCTTCACCTTTGGCTTCGGCGGCGTGCTGTT
CCCGATGCATATCGGCGCCTCCTATGTCGTGCTGGAAAAGACTTCGCCGGACGACCTGCTCTCTGCCATCGAGCGCTATA
GAGCGACGGTCTGCTTCACGGCGCCCACAGCGTATCGTGCGATGATCGGAAAGCTCGCAGGCCGCGACATCTCCTCTCTG
CGCAAATGCGTCTCCGCCGGCGAGACGCTGCCGAAGCCGACCTTCGATGCCTGGCTCAAGGCCACCGGGCTCAAGCTGAT
GGACGGCATCGGCTCGACCGAGATGCTGCACATCTTCATCAGCGCCGTTGAGGACGAGATCCGCCCCGGCGCCACCGGCA
AGCCCGTACCGGGCTATGAGGCGAAGATCGTCGACGACGAGGGCCGCGACGTGCCGCCGGGCACGATGGGCCGGCTCGCC
GTCCGCGGCCCCACCGGCTGCCGCTATCTCGCCGACGAGCGCCAGCGCAAATATGTCCAGAACGGCTGGAACGTCACCGG
CGACACCTATGTGATGGATGAGGACGGCTACTTCTGGTACCAGTCGCGCTCCGACGACATGATCGTGTCGTCGGGCTACA
ACATCGCAGGCACCGATGTCGAAGCCGCCCTGCTGACGCATCCGACCGTGGCCGAATGCGGTGTGGTCGGTGCGCCGGAC
GAGGCCCGCGGCATGGTCGTGAAGGCCTATGTCGTGCTGGCGCCGGGTGCGGAAGGTACAACGGCGCTGGTGGCCGAGCT
GCAGGAGCACGTCAAACGCGAGATCGCGCCGTACAAATATCCGCGCGCGATTGAGTTCGTGACGCAGCTTCCCAAGACAG
AGACCGGCAAGCTGAAGCGGTTCGCCCTGCGGCAGATCGCGCAGGCCGCGGCTGCGCCTGGCCCGGCCGCGTAG

Upstream 100 bases:

>100_bases
TCTTCGGCTCTCTTCGCCTCTTGCGTCCAATTGTTTTAAGTCTAAAATGTTTAGGACTGAAACGTGCCGGGCGTCCTTCC
CTTAGAGACAGGAGCGTGTG

Downstream 100 bases:

>100_bases
CAGAGACAAGGAGAGACGTTCGTGAGCACGACCAAAGCGCCCACGCTCGCCGTGGTGAAGCAGGACGATTCGAGCCTGCG
CGTGCTGCAGCCGAGCGGCT

Product: putative acid-thiol ligase

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MATAAKVQQPESAHSAHVDTFAQDNLPPREMWPDFVFSRPELQYPPRLNCVTHFLDRWVEEGRGDAPCIISTEVSYTYRE
LQALVNKIANVLVGKLGLVPGGRVLLRSANNPMMVATYLAAIKAGGIVVATMPLLRAKELSYPIQKAQIALALCDGKLAD
EMEKTRAVAPALKQVVYWGNGQPDALEALIADASPDFAALDTASDDLCLIAFTSGTTGDPKGTMHFHRDMLAVCDGYARN
VLRASQSDRFIGTAPLAFTFGFGGVLFPMHIGASYVVLEKTSPDDLLSAIERYRATVCFTAPTAYRAMIGKLAGRDISSL
RKCVSAGETLPKPTFDAWLKATGLKLMDGIGSTEMLHIFISAVEDEIRPGATGKPVPGYEAKIVDDEGRDVPPGTMGRLA
VRGPTGCRYLADERQRKYVQNGWNVTGDTYVMDEDGYFWYQSRSDDMIVSSGYNIAGTDVEAALLTHPTVAECGVVGAPD
EARGMVVKAYVVLAPGAEGTTALVAELQEHVKREIAPYKYPRAIEFVTQLPKTETGKLKRFALRQIAQAAAAPGPAA

Sequences:

>Translated_557_residues
MATAAKVQQPESAHSAHVDTFAQDNLPPREMWPDFVFSRPELQYPPRLNCVTHFLDRWVEEGRGDAPCIISTEVSYTYRE
LQALVNKIANVLVGKLGLVPGGRVLLRSANNPMMVATYLAAIKAGGIVVATMPLLRAKELSYPIQKAQIALALCDGKLAD
EMEKTRAVAPALKQVVYWGNGQPDALEALIADASPDFAALDTASDDLCLIAFTSGTTGDPKGTMHFHRDMLAVCDGYARN
VLRASQSDRFIGTAPLAFTFGFGGVLFPMHIGASYVVLEKTSPDDLLSAIERYRATVCFTAPTAYRAMIGKLAGRDISSL
RKCVSAGETLPKPTFDAWLKATGLKLMDGIGSTEMLHIFISAVEDEIRPGATGKPVPGYEAKIVDDEGRDVPPGTMGRLA
VRGPTGCRYLADERQRKYVQNGWNVTGDTYVMDEDGYFWYQSRSDDMIVSSGYNIAGTDVEAALLTHPTVAECGVVGAPD
EARGMVVKAYVVLAPGAEGTTALVAELQEHVKREIAPYKYPRAIEFVTQLPKTETGKLKRFALRQIAQAAAAPGPAA
>Mature_556_residues
ATAAKVQQPESAHSAHVDTFAQDNLPPREMWPDFVFSRPELQYPPRLNCVTHFLDRWVEEGRGDAPCIISTEVSYTYREL
QALVNKIANVLVGKLGLVPGGRVLLRSANNPMMVATYLAAIKAGGIVVATMPLLRAKELSYPIQKAQIALALCDGKLADE
MEKTRAVAPALKQVVYWGNGQPDALEALIADASPDFAALDTASDDLCLIAFTSGTTGDPKGTMHFHRDMLAVCDGYARNV
LRASQSDRFIGTAPLAFTFGFGGVLFPMHIGASYVVLEKTSPDDLLSAIERYRATVCFTAPTAYRAMIGKLAGRDISSLR
KCVSAGETLPKPTFDAWLKATGLKLMDGIGSTEMLHIFISAVEDEIRPGATGKPVPGYEAKIVDDEGRDVPPGTMGRLAV
RGPTGCRYLADERQRKYVQNGWNVTGDTYVMDEDGYFWYQSRSDDMIVSSGYNIAGTDVEAALLTHPTVAECGVVGAPDE
ARGMVVKAYVVLAPGAEGTTALVAELQEHVKREIAPYKYPRAIEFVTQLPKTETGKLKRFALRQIAQAAAAPGPAA

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=535, Percent_Identity=32.8971962616822, Blast_Score=266, Evalue=5e-71,
Organism=Homo sapiens, GI38505220, Length=487, Percent_Identity=34.2915811088296, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI122937307, Length=508, Percent_Identity=34.6456692913386, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI115511026, Length=542, Percent_Identity=30.9963099630996, Blast_Score=233, Evalue=5e-61,
Organism=Homo sapiens, GI58082049, Length=536, Percent_Identity=30.9701492537313, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI157311624, Length=527, Percent_Identity=30.7400379506641, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI157311622, Length=527, Percent_Identity=30.7400379506641, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI187761345, Length=533, Percent_Identity=27.7673545966229, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI187761343, Length=533, Percent_Identity=27.7673545966229, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI13375727, Length=557, Percent_Identity=26.9299820466786, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI156151445, Length=533, Percent_Identity=28.7054409005629, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI28416953, Length=583, Percent_Identity=27.6157804459691, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI8923896, Length=644, Percent_Identity=25.1552795031056, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI253970489, Length=415, Percent_Identity=28.6746987951807, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI195947378, Length=441, Percent_Identity=25.1700680272109, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI42544134, Length=370, Percent_Identity=26.4864864864865, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI145693145, Length=511, Percent_Identity=31.7025440313112, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788107, Length=528, Percent_Identity=28.030303030303, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1790505, Length=547, Percent_Identity=27.7879341864717, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI221142682, Length=486, Percent_Identity=27.3662551440329, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1786529, Length=568, Percent_Identity=26.4084507042254, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786810, Length=500, Percent_Identity=26.2, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789201, Length=359, Percent_Identity=25.9052924791086, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788595, Length=489, Percent_Identity=24.1308793456033, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1786801, Length=537, Percent_Identity=25.512104283054, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538037, Length=529, Percent_Identity=25.7088846880907, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17557194, Length=527, Percent_Identity=26.1859582542694, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI71994694, Length=526, Percent_Identity=29.6577946768061, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI71994703, Length=526, Percent_Identity=29.6577946768061, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71994690, Length=526, Percent_Identity=29.6577946768061, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71982997, Length=571, Percent_Identity=27.1453590192644, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71983001, Length=571, Percent_Identity=27.1453590192644, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=536, Percent_Identity=28.1716417910448, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=491, Percent_Identity=24.847250509165, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71996755, Length=492, Percent_Identity=25.4065040650407, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=364, Percent_Identity=28.021978021978, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17558820, Length=476, Percent_Identity=23.5294117647059, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=479, Percent_Identity=23.1732776617954, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=530, Percent_Identity=23.3962264150943, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=346, Percent_Identity=26.5895953757225, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=527, Percent_Identity=27.134724857685, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323182, Length=387, Percent_Identity=31.266149870801, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319699, Length=355, Percent_Identity=27.887323943662, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24667955, Length=566, Percent_Identity=27.208480565371, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24581924, Length=535, Percent_Identity=26.7289719626168, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI62472339, Length=531, Percent_Identity=27.4952919020716, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24667959, Length=436, Percent_Identity=27.7522935779816, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI18859661, Length=525, Percent_Identity=26.6666666666667, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21355181, Length=515, Percent_Identity=24.8543689320388, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI21355225, Length=570, Percent_Identity=25.0877192982456, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24648260, Length=480, Percent_Identity=23.9583333333333, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356947, Length=507, Percent_Identity=24.4575936883629, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653035, Length=502, Percent_Identity=24.1035856573705, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922652, Length=482, Percent_Identity=22.4066390041494, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648253, Length=464, Percent_Identity=24.7844827586207, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648255, Length=464, Percent_Identity=25, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI20130357, Length=368, Percent_Identity=24.4565217391304, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648257, Length=436, Percent_Identity=24.3119266055046, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21356441, Length=489, Percent_Identity=24.5398773006135, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI161076582, Length=498, Percent_Identity=21.8875502008032, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 60210; Mature: 60078

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAAKVQQPESAHSAHVDTFAQDNLPPREMWPDFVFSRPELQYPPRLNCVTHFLDRWVE
CCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHH
EGRGDAPCIISTEVSYTYRELQALVNKIANVLVGKLGLVPGGRVLLRSANNPMMVATYLA
HCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHH
AIKAGGIVVATMPLLRAKELSYPIQKAQIALALCDGKLADEMEKTRAVAPALKQVVYWGN
HHHCCCEEEEEHHHHHHHHHCCCHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHEECCC
GQPDALEALIADASPDFAALDTASDDLCLIAFTSGTTGDPKGTMHFHRDMLAVCDGYARN
CCHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
VLRASQSDRFIGTAPLAFTFGFGGVLFPMHIGASYVVLEKTSPDDLLSAIERYRATVCFT
HHHHCCCCCEEECCCHHEECCCCCHHHHHHCCCEEEEEECCCHHHHHHHHHHHCEEEEEE
APTAYRAMIGKLAGRDISSLRKCVSAGETLPKPTFDAWLKATGLKLMDGIGSTEMLHIFI
CCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHCCCCHHHHHHHH
SAVEDEIRPGATGKPVPGYEAKIVDDEGRDVPPGTMGRLAVRGPTGCRYLADERQRKYVQ
HHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
NGWNVTGDTYVMDEDGYFWYQSRSDDMIVSSGYNIAGTDVEAALLTHPTVAECGVVGAPD
CCCCCCCCEEEEECCCEEEEECCCCCEEEECCCCEECCCCCHHHHCCCCHHHCCCCCCCC
EARGMVVKAYVVLAPGAEGTTALVAELQEHVKREIAPYKYPRAIEFVTQLPKTETGKLKR
HHCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHH
FALRQIAQAAAAPGPAA
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ATAAKVQQPESAHSAHVDTFAQDNLPPREMWPDFVFSRPELQYPPRLNCVTHFLDRWVE
CCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHH
EGRGDAPCIISTEVSYTYRELQALVNKIANVLVGKLGLVPGGRVLLRSANNPMMVATYLA
HCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHH
AIKAGGIVVATMPLLRAKELSYPIQKAQIALALCDGKLADEMEKTRAVAPALKQVVYWGN
HHHCCCEEEEEHHHHHHHHHCCCHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHEECCC
GQPDALEALIADASPDFAALDTASDDLCLIAFTSGTTGDPKGTMHFHRDMLAVCDGYARN
CCHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
VLRASQSDRFIGTAPLAFTFGFGGVLFPMHIGASYVVLEKTSPDDLLSAIERYRATVCFT
HHHHCCCCCEEECCCHHEECCCCCHHHHHHCCCEEEEEECCCHHHHHHHHHHHCEEEEEE
APTAYRAMIGKLAGRDISSLRKCVSAGETLPKPTFDAWLKATGLKLMDGIGSTEMLHIFI
CCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHCCCCHHHHHHHH
SAVEDEIRPGATGKPVPGYEAKIVDDEGRDVPPGTMGRLAVRGPTGCRYLADERQRKYVQ
HHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
NGWNVTGDTYVMDEDGYFWYQSRSDDMIVSSGYNIAGTDVEAALLTHPTVAECGVVGAPD
CCCCCCCCEEEEECCCEEEEECCCCCEEEECCCCEECCCCCHHHHCCCCHHHCCCCCCCC
EARGMVVKAYVVLAPGAEGTTALVAELQEHVKREIAPYKYPRAIEFVTQLPKTETGKLKR
HHCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHH
FALRQIAQAAAAPGPAA
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]