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The map label for this gene is livK [C]

Identifier: 146339344

GI number: 146339344

Start: 2478054

End: 2479226

Strand: Reverse

Name: livK [C]

Synonym: BRADO2325

Alternate gene names: 146339344

Gene position: 2479226-2478054 (Counterclockwise)

Preceding gene: 146339349

Following gene: 146339343

Centisome position: 33.25

GC content: 63.09

Gene sequence:

>1173_bases
ATGAAAGTGAGTTCGAAGCTGGCCGGTCTCGGCGTATTGGCCTGCCTGCTGGCGCAGCCAGCGCTGGCCGCCGACAAGAT
CAAGATCGGCGTCATCGTCACCCTGTCCGGCCCCGCGGCCGCGCTCGGCGGACAGGTCCGCGACGGCTTCAACCTTGCGA
TCAAGGACCTCGGCGGCAAGATGGGCGGTAAGGACGTCGAGGTGGTCGTCGCCGACGACGAGCTCAAGCCTGACGCCGCC
ATCACCAAGGCCAAGGGCCTGCTCGAGCGCGACAAGGTCGACTTCGTTGTCGGTCCGATCTTCTCCAACATGTTGCAGGC
GATCCACCGCCCGATCACCGAGAACAAGACCTTCCTGATCAGCCCGAACGCCGGCCCCTCCAGCTACGCGGGCAAGGAGT
GCAGCCCCTATTTCTACGTGACCTCGTACCAGAACGATCAGGTGCATGCGGTGCTCGGCAAGGTCGCCCAGGATCGCGGC
TACAAGAAGATGTATCTCCTGGTGCCGAATTATCAGGCTGGTCGCGACAGCGTCGCCGGGTTCAAGCTCGACTACAAGGG
CGAGATCGTCGAGGAGTCCTACGTTCCGCTGAACACGCTCGATTTCCAGCCCGAGCTGTCGAAAATCTCCTCGCAGAAGC
CGGACGCCGTGTTCACCTTCATGCCGGGCGGCATGGGCGTGAACCTGGTCAAGCAGTTCAAGCAGGCTGGCCTCGCCGAT
ACCGTTCCGGTGCTCTCGGCGTTCACCGTCGACGAATCCACCCTGCCCGCGCAGCAGGACGCCGCGGTCGGCATGTTCGG
CGGCGCCAACTGGGCGCCCAATTTCGACAATCCGCAGAGCAAGAAGTTCGTCGCCGGCTACGAGGCCGCCTATAACAGCG
TACCCGGCACCTACGCGATGCAGGCCTATGACGCCGCGCTCTTGATCGACAGCACCGTGAGGGCGGTCGGCGGCGACCTC
TCGGACAAGGACAAGGTGGCGGGCGCGCTCAAGAAGGCCGACTTTGCCTCGCTGCGCGGCAACTTCAAGTTCAACACCAA
CGGCTATCCGATCCAGGACTTCTATCTGACCAAGGTCGCCAAGCGTCCCGACGGCAAGTTCCAGACGGAGATCGTGCAGA
AGGTGTTCGAGAACTACGCCGACCGCTACGCTAGGGATTGCGCGGCCAAGTAA

Upstream 100 bases:

>100_bases
GCGTCATCAGACTAGGCTTGGTCCCAAGCTGCGGTCTGACCATCATTCGGTCAGGTGCAAAAAACATAAACCTGAATTTC
CGCATCCCGGGGGAGTTGTC

Downstream 100 bases:

>100_bases
TAACAGGGAGCGAAACCACCATGAGCCATCCGGAAATCGCCGGCATCACCCGGGCCAATGAGGGCATCCAGGGTATCTCC
TGGAACATCCTCGGCCAGAT

Product: putative ABC transporter substrate binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKVSSKLAGLGVLACLLAQPALAADKIKIGVIVTLSGPAAALGGQVRDGFNLAIKDLGGKMGGKDVEVVVADDELKPDAA
ITKAKGLLERDKVDFVVGPIFSNMLQAIHRPITENKTFLISPNAGPSSYAGKECSPYFYVTSYQNDQVHAVLGKVAQDRG
YKKMYLLVPNYQAGRDSVAGFKLDYKGEIVEESYVPLNTLDFQPELSKISSQKPDAVFTFMPGGMGVNLVKQFKQAGLAD
TVPVLSAFTVDESTLPAQQDAAVGMFGGANWAPNFDNPQSKKFVAGYEAAYNSVPGTYAMQAYDAALLIDSTVRAVGGDL
SDKDKVAGALKKADFASLRGNFKFNTNGYPIQDFYLTKVAKRPDGKFQTEIVQKVFENYADRYARDCAAK

Sequences:

>Translated_390_residues
MKVSSKLAGLGVLACLLAQPALAADKIKIGVIVTLSGPAAALGGQVRDGFNLAIKDLGGKMGGKDVEVVVADDELKPDAA
ITKAKGLLERDKVDFVVGPIFSNMLQAIHRPITENKTFLISPNAGPSSYAGKECSPYFYVTSYQNDQVHAVLGKVAQDRG
YKKMYLLVPNYQAGRDSVAGFKLDYKGEIVEESYVPLNTLDFQPELSKISSQKPDAVFTFMPGGMGVNLVKQFKQAGLAD
TVPVLSAFTVDESTLPAQQDAAVGMFGGANWAPNFDNPQSKKFVAGYEAAYNSVPGTYAMQAYDAALLIDSTVRAVGGDL
SDKDKVAGALKKADFASLRGNFKFNTNGYPIQDFYLTKVAKRPDGKFQTEIVQKVFENYADRYARDCAAK
>Mature_390_residues
MKVSSKLAGLGVLACLLAQPALAADKIKIGVIVTLSGPAAALGGQVRDGFNLAIKDLGGKMGGKDVEVVVADDELKPDAA
ITKAKGLLERDKVDFVVGPIFSNMLQAIHRPITENKTFLISPNAGPSSYAGKECSPYFYVTSYQNDQVHAVLGKVAQDRG
YKKMYLLVPNYQAGRDSVAGFKLDYKGEIVEESYVPLNTLDFQPELSKISSQKPDAVFTFMPGGMGVNLVKQFKQAGLAD
TVPVLSAFTVDESTLPAQQDAAVGMFGGANWAPNFDNPQSKKFVAGYEAAYNSVPGTYAMQAYDAALLIDSTVRAVGGDL
SDKDKVAGALKKADFASLRGNFKFNTNGYPIQDFYLTKVAKRPDGKFQTEIVQKVFENYADRYARDCAAK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41923; Mature: 41923

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSSKLAGLGVLACLLAQPALAADKIKIGVIVTLSGPAAALGGQVRDGFNLAIKDLGGK
CCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHCCCCCCCCEEEEEHHCCC
MGGKDVEVVVADDELKPDAAITKAKGLLERDKVDFVVGPIFSNMLQAIHRPITENKTFLI
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCEEEE
SPNAGPSSYAGKECSPYFYVTSYQNDQVHAVLGKVAQDRGYKKMYLLVPNYQAGRDSVAG
ECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
FKLDYKGEIVEESYVPLNTLDFQPELSKISSQKPDAVFTFMPGGMGVNLVKQFKQAGLAD
EEECCCCCEECCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCH
TVPVLSAFTVDESTLPAQQDAAVGMFGGANWAPNFDNPQSKKFVAGYEAAYNSVPGTYAM
HHHHHEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCHH
QAYDAALLIDSTVRAVGGDLSDKDKVAGALKKADFASLRGNFKFNTNGYPIQDFYLTKVA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
KRPDGKFQTEIVQKVFENYADRYARDCAAK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVSSKLAGLGVLACLLAQPALAADKIKIGVIVTLSGPAAALGGQVRDGFNLAIKDLGGK
CCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHCCCCCCCCEEEEEHHCCC
MGGKDVEVVVADDELKPDAAITKAKGLLERDKVDFVVGPIFSNMLQAIHRPITENKTFLI
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCEEEE
SPNAGPSSYAGKECSPYFYVTSYQNDQVHAVLGKVAQDRGYKKMYLLVPNYQAGRDSVAG
ECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
FKLDYKGEIVEESYVPLNTLDFQPELSKISSQKPDAVFTFMPGGMGVNLVKQFKQAGLAD
EEECCCCCEECCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCH
TVPVLSAFTVDESTLPAQQDAAVGMFGGANWAPNFDNPQSKKFVAGYEAAYNSVPGTYAM
HHHHHEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCHH
QAYDAALLIDSTVRAVGGDLSDKDKVAGALKKADFASLRGNFKFNTNGYPIQDFYLTKVA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
KRPDGKFQTEIVQKVFENYADRYARDCAAK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA