Click here to switch to the map view.

The map label for this gene is iolD1 [H]

Identifier: 146339269

GI number: 146339269

Start: 2389799

End: 2391637

Strand: Reverse

Name: iolD1 [H]

Synonym: BRADO2243

Alternate gene names: 146339269

Gene position: 2391637-2389799 (Counterclockwise)

Preceding gene: 146339271

Following gene: 146339268

Centisome position: 32.07

GC content: 68.68

Gene sequence:

>1839_bases
ATGGCGACGATCAGGCTGACGATGGCGCAGGCGCTCGTCGCCGCGATCGCGGCGCAGCGGACGGTGATCGACGGCGAGAC
GGTGCCGCTGTTCGCCGGCGTCTGGGCGATCTTCGGCCATGGCAATGTGGCCGGTCTCGGCGAGGCGCTGTTTGCCGTGC
GCGATCGCCTGCCCACCTTGCGCGCGCATCATGAGCAGGCGATGGCCCATGCCGCGATCGCATTTGCCAAGGCCGCGCGC
CGCCGGCGCATGATGGCCGTGACGAGCTCGATCGGCCCCGGGGCGACCAACATGGTGACAGCCGCAGCCGTCGCGCATGT
CAACCGTCTGCCGCTCTTGCTGCTGCCGGGCGATGTCTTCGCCGGACGGCGTCCCGACCCGGTGTTGCAGCAGATCGAGG
ATTTTGGCGACGGCACTGTCTCGGCCAATGATTGCTTCCGCCCGGTCTCGCGCTATTTCGACCGGATCACCCGGCCCGAG
CAGATCATCCCGGCCTTCGAGCGTGCGATGCAGGTTCTGACCGACCCCGCCGAATGCGGCCCGGTGACTCTCGCGCTGTG
CCAGGACGTGCAGACGGAGGCCTACGACTTTCCGTCTCAATTCTTCGCCGAGCGCATCTGGCAGCCGTGCCGGCCGCGGC
CCGACGCGACGCGACTGGCCGAGACTGCAACCCTCATCAAGACATCCAAGCGCCCGCTCGTCATCGCCGGCGGCGGCGTC
CTCTACAGCGAGGCGGAACGCGACCTCGCCGAGTTCTGCCTGACGCACGGCGTTCCCGCGGCGGAGACGCAGGCCGGCAA
GAGCGCACTGCCTCACGACCATCCGCTCAATCTCGGCGCGATCGGCGTCACCGGCACCGGCGCGGCCAATGCGGCTGCGC
AGCAGGCCGATGTGGTGCTCGCGATCGGCACCCGGCTGCAGGACTTCACCACCGGCTCGCGCGCGCTGTTCGCCGAGGCC
TGCCGCATCATCGGCCTCAACACGCAGCCGTTCGATGCCGGCAAGCACCGCGCGCAGCCGCTGGTCGCCGATGCCAGGGC
CGGACTGGCGGAGCTCGGTACAGCCCTTCATGGCTGGACGGCGCCTGCCACCTGGACCGAACAGGCAAAAGCCGGCCGCA
CGGCCTGGCTGGACACCGCGGCGCGCTATCTTGCGGCTAGCAACGCGGCCTTGCCCAGCGATGCCGAGGTGATCGGTGCC
GTGCAACGCCGCAGCCGGCCCGACGACGTGGTGCTGTGCGCCGCAGGCGGCTTGCCCGGCGAGTTGCACAAGCTGTGGCA
GCCGGGCGCGCCCGGCGGCTATCATATGGAATACGGCTATTCGTGCATGGGCTATGAGATCGCCGGCGGCCTGGGCGCCA
AGCTCGCCGATCCCGCCCGAGACGTCATCGTCATGGTCGGCGACGGATCCTATCTGATGATGAATTCCGAGATCGCCACG
TCGATCATGCTCGGGGCAAAACTGATCATCGTCGTGCTCGACAATCGCGGTTTCGGCTGCATCAACCGGCTCCAGAACGC
GACCGGAGGTGCCCCGTTCAACAACCTGCTGCGCGACACGCGTCACGTGACCTTGCCCGAGATCGACTTCGCGAGCCACG
CAGCCAGCCTCGGCGCGATCGCGCACAAGGTCGCCGGGATCACCGAGCTCGAAGCGGCGCTCGATGCGGCGCGCGGCCAC
GATCGCACCAGCGTCATCGTGATCGACACCGACCCACTGCGGTCTACCGATGCCGGCGGCCATTGGTGGGAGGTGGCGGT
GCCGGAGGTCTCGGAGCGCGCGGAGATCGAGGCATCCAGACGCAACTATATCGAGGCGCTGCGGCGCCGCGGCCATTGA

Upstream 100 bases:

>100_bases
GTGAGCGCGCGCGCTTTTGCAAAGGCATGCGCAGAGCCCAGTGCTGCATCATGTCGGCGGGCCTGATCATGTTGCGTGCT
GGGTTGAGCAGGAGCGAATA

Downstream 100 bases:

>100_bases
TCCAGCGAGGACGACATGCCCATCCGTATCGGCGCGAATCCCATCGGGTGGTCGAACGACGATCTGCAGGAGATCGGCGG
CGAGACGCCGCTCGAGACCT

Product: putative malonic semialdehyde oxidative decarboxylase

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase 1 [H]

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MATIRLTMAQALVAAIAAQRTVIDGETVPLFAGVWAIFGHGNVAGLGEALFAVRDRLPTLRAHHEQAMAHAAIAFAKAAR
RRRMMAVTSSIGPGATNMVTAAAVAHVNRLPLLLLPGDVFAGRRPDPVLQQIEDFGDGTVSANDCFRPVSRYFDRITRPE
QIIPAFERAMQVLTDPAECGPVTLALCQDVQTEAYDFPSQFFAERIWQPCRPRPDATRLAETATLIKTSKRPLVIAGGGV
LYSEAERDLAEFCLTHGVPAAETQAGKSALPHDHPLNLGAIGVTGTGAANAAAQQADVVLAIGTRLQDFTTGSRALFAEA
CRIIGLNTQPFDAGKHRAQPLVADARAGLAELGTALHGWTAPATWTEQAKAGRTAWLDTAARYLAASNAALPSDAEVIGA
VQRRSRPDDVVLCAAGGLPGELHKLWQPGAPGGYHMEYGYSCMGYEIAGGLGAKLADPARDVIVMVGDGSYLMMNSEIAT
SIMLGAKLIIVVLDNRGFGCINRLQNATGGAPFNNLLRDTRHVTLPEIDFASHAASLGAIAHKVAGITELEAALDAARGH
DRTSVIVIDTDPLRSTDAGGHWWEVAVPEVSERAEIEASRRNYIEALRRRGH

Sequences:

>Translated_612_residues
MATIRLTMAQALVAAIAAQRTVIDGETVPLFAGVWAIFGHGNVAGLGEALFAVRDRLPTLRAHHEQAMAHAAIAFAKAAR
RRRMMAVTSSIGPGATNMVTAAAVAHVNRLPLLLLPGDVFAGRRPDPVLQQIEDFGDGTVSANDCFRPVSRYFDRITRPE
QIIPAFERAMQVLTDPAECGPVTLALCQDVQTEAYDFPSQFFAERIWQPCRPRPDATRLAETATLIKTSKRPLVIAGGGV
LYSEAERDLAEFCLTHGVPAAETQAGKSALPHDHPLNLGAIGVTGTGAANAAAQQADVVLAIGTRLQDFTTGSRALFAEA
CRIIGLNTQPFDAGKHRAQPLVADARAGLAELGTALHGWTAPATWTEQAKAGRTAWLDTAARYLAASNAALPSDAEVIGA
VQRRSRPDDVVLCAAGGLPGELHKLWQPGAPGGYHMEYGYSCMGYEIAGGLGAKLADPARDVIVMVGDGSYLMMNSEIAT
SIMLGAKLIIVVLDNRGFGCINRLQNATGGAPFNNLLRDTRHVTLPEIDFASHAASLGAIAHKVAGITELEAALDAARGH
DRTSVIVIDTDPLRSTDAGGHWWEVAVPEVSERAEIEASRRNYIEALRRRGH
>Mature_611_residues
ATIRLTMAQALVAAIAAQRTVIDGETVPLFAGVWAIFGHGNVAGLGEALFAVRDRLPTLRAHHEQAMAHAAIAFAKAARR
RRMMAVTSSIGPGATNMVTAAAVAHVNRLPLLLLPGDVFAGRRPDPVLQQIEDFGDGTVSANDCFRPVSRYFDRITRPEQ
IIPAFERAMQVLTDPAECGPVTLALCQDVQTEAYDFPSQFFAERIWQPCRPRPDATRLAETATLIKTSKRPLVIAGGGVL
YSEAERDLAEFCLTHGVPAAETQAGKSALPHDHPLNLGAIGVTGTGAANAAAQQADVVLAIGTRLQDFTTGSRALFAEAC
RIIGLNTQPFDAGKHRAQPLVADARAGLAELGTALHGWTAPATWTEQAKAGRTAWLDTAARYLAASNAALPSDAEVIGAV
QRRSRPDDVVLCAAGGLPGELHKLWQPGAPGGYHMEYGYSCMGYEIAGGLGAKLADPARDVIVMVGDGSYLMMNSEIATS
IMLGAKLIIVVLDNRGFGCINRLQNATGGAPFNNLLRDTRHVTLPEIDFASHAASLGAIAHKVAGITELEAALDAARGHD
RTSVIVIDTDPLRSTDAGGHWWEVAVPEVSERAEIEASRRNYIEALRRRGH

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=551, Percent_Identity=21.7785843920145, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=26.1101243339254, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1790104, Length=462, Percent_Identity=25.3246753246753, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1786717, Length=535, Percent_Identity=24.6728971962617, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1787096, Length=490, Percent_Identity=22.6530612244898, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788716, Length=504, Percent_Identity=23.015873015873, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=23.4991423670669, Blast_Score=111, Evalue=4e-25,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 65126; Mature: 64995

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIRLTMAQALVAAIAAQRTVIDGETVPLFAGVWAIFGHGNVAGLGEALFAVRDRLPTL
CCEEEHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
RAHHEQAMAHAAIAFAKAARRRRMMAVTSSIGPGATNMVTAAAVAHVNRLPLLLLPGDVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHC
AGRRPDPVLQQIEDFGDGTVSANDCFRPVSRYFDRITRPEQIIPAFERAMQVLTDPAECG
CCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
PVTLALCQDVQTEAYDFPSQFFAERIWQPCRPRPDATRLAETATLIKTSKRPLVIAGGGV
CCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCE
LYSEAERDLAEFCLTHGVPAAETQAGKSALPHDHPLNLGAIGVTGTGAANAAAQQADVVL
EEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCEEE
AIGTRLQDFTTGSRALFAEACRIIGLNTQPFDAGKHRAQPLVADARAGLAELGTALHGWT
EECCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHCCHHHHHHHHCCCC
APATWTEQAKAGRTAWLDTAARYLAASNAALPSDAEVIGAVQRRSRPDDVVLCAAGGLPG
CCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCH
ELHKLWQPGAPGGYHMEYGYSCMGYEIAGGLGAKLADPARDVIVMVGDGSYLMMNSEIAT
HHHHHCCCCCCCCCEEECCCEEECEEECCCCCCCCCCCCCCEEEEECCCCEEEECHHHHH
SIMLGAKLIIVVLDNRGFGCINRLQNATGGAPFNNLLRDTRHVTLPEIDFASHAASLGAI
HHHHCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AHKVAGITELEAALDAARGHDRTSVIVIDTDPLRSTDAGGHWWEVAVPEVSERAEIEASR
HHHHHCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
RNYIEALRRRGH
HHHHHHHHHCCC
>Mature Secondary Structure 
ATIRLTMAQALVAAIAAQRTVIDGETVPLFAGVWAIFGHGNVAGLGEALFAVRDRLPTL
CEEEHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
RAHHEQAMAHAAIAFAKAARRRRMMAVTSSIGPGATNMVTAAAVAHVNRLPLLLLPGDVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHC
AGRRPDPVLQQIEDFGDGTVSANDCFRPVSRYFDRITRPEQIIPAFERAMQVLTDPAECG
CCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
PVTLALCQDVQTEAYDFPSQFFAERIWQPCRPRPDATRLAETATLIKTSKRPLVIAGGGV
CCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCE
LYSEAERDLAEFCLTHGVPAAETQAGKSALPHDHPLNLGAIGVTGTGAANAAAQQADVVL
EEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCEEE
AIGTRLQDFTTGSRALFAEACRIIGLNTQPFDAGKHRAQPLVADARAGLAELGTALHGWT
EECCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHCCHHHHHHHHCCCC
APATWTEQAKAGRTAWLDTAARYLAASNAALPSDAEVIGAVQRRSRPDDVVLCAAGGLPG
CCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCH
ELHKLWQPGAPGGYHMEYGYSCMGYEIAGGLGAKLADPARDVIVMVGDGSYLMMNSEIAT
HHHHHCCCCCCCCCEEECCCEEECEEECCCCCCCCCCCCCCEEEEECCCCEEEECHHHHH
SIMLGAKLIIVVLDNRGFGCINRLQNATGGAPFNNLLRDTRHVTLPEIDFASHAASLGAI
HHHHCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AHKVAGITELEAALDAARGHDRTSVIVIDTDPLRSTDAGGHWWEVAVPEVSERAEIEASR
HHHHHCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
RNYIEALRRRGH
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA