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The map label for this gene is paaF [C]

Identifier: 146339207

GI number: 146339207

Start: 2304423

End: 2305187

Strand: Reverse

Name: paaF [C]

Synonym: BRADO2169

Alternate gene names: 146339207

Gene position: 2305187-2304423 (Counterclockwise)

Preceding gene: 146339208

Following gene: 146339206

Centisome position: 30.91

GC content: 65.23

Gene sequence:

>765_bases
ATGACCGACCATCTGATCGTCACCGACGAGGGCGAGACGCGGACGATCAAGCTCAGGCGTCCCGAGAAGAAGAACGCGAT
CACGCAGGCCATGTATCTCGGCATGAGCCATGCGATCGACACCGCGCAGAACAATCCGCTGGTGCGATGCATCATCATCA
CCGGCGGCTCCGGCGTGTTCACCGCTGGCAACGACCTCGAGGATTTCCTGCGGGCCGGCGATGCCGGCAGCGAGGCGGCG
CGCACCTCGGCCGCGACCAAATTCCTGTATTCGCTGGCCCACAACGTCAAGCCGATCATCGCCGCGGTTGACGGCGTCGC
GATCGGCATCGGCACCACGATGCTGTTCCACTGCGACTATGTGCTGGCCTCGACCACGGCGACATTCTCGACGCCCTTCA
TCCATCTCGGCCTGGTGCCGGAGGGCGCCTCGAGCCTGCTGATGCCACAGACCATGGGCTATCAGCGCGCGTTCGCGATG
CTGGTGATGGGCCGCACCATGACCGCCGCGGATGCGCATATCGCCGGCTTCGTCAACACCGTGGTGGCGCCGGGTCATAC
CGAGGTCGAGGCGCACAAGGTGGCGCGCGACATCGCGCGGCTGCCCGCCGAGGCGGTCGCGATCGGACGCAAGTTGATCC
GCACCGCGCCGGACGAATTGACCCGCCGCATCGACCAGGAGGCCTATCTGTTCGCCGAGCGGATGACCTCGGAAGAGGCG
ATCGGCGCGTTCAAGGCGTTCCTGTCGCGCAAGAAGAAGAAATGA

Upstream 100 bases:

>100_bases
ACGTTCAGCTCTTGGCGTGACCGGGGGATGATGTAGGTTGGCGGGAGAGAAACGACTCGATCGTCACCGCCGGCGGCCGA
CGCACATGCATGAGGGCTCT

Downstream 100 bases:

>100_bases
CGGCGGGGCCGTCGAACGGGCATGGCGCAGTTTGATGTCATCGTGATCGGCTGCGGCGCGGTCGGCAGCGCCGCGCTGCT
GCATCTCGCCAGAGCGGGAA

Product: putative enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTDHLIVTDEGETRTIKLRRPEKKNAITQAMYLGMSHAIDTAQNNPLVRCIIITGGSGVFTAGNDLEDFLRAGDAGSEAA
RTSAATKFLYSLAHNVKPIIAAVDGVAIGIGTTMLFHCDYVLASTTATFSTPFIHLGLVPEGASSLLMPQTMGYQRAFAM
LVMGRTMTAADAHIAGFVNTVVAPGHTEVEAHKVARDIARLPAEAVAIGRKLIRTAPDELTRRIDQEAYLFAERMTSEEA
IGAFKAFLSRKKKK

Sequences:

>Translated_254_residues
MTDHLIVTDEGETRTIKLRRPEKKNAITQAMYLGMSHAIDTAQNNPLVRCIIITGGSGVFTAGNDLEDFLRAGDAGSEAA
RTSAATKFLYSLAHNVKPIIAAVDGVAIGIGTTMLFHCDYVLASTTATFSTPFIHLGLVPEGASSLLMPQTMGYQRAFAM
LVMGRTMTAADAHIAGFVNTVVAPGHTEVEAHKVARDIARLPAEAVAIGRKLIRTAPDELTRRIDQEAYLFAERMTSEEA
IGAFKAFLSRKKKK
>Mature_253_residues
TDHLIVTDEGETRTIKLRRPEKKNAITQAMYLGMSHAIDTAQNNPLVRCIIITGGSGVFTAGNDLEDFLRAGDAGSEAAR
TSAATKFLYSLAHNVKPIIAAVDGVAIGIGTTMLFHCDYVLASTTATFSTPFIHLGLVPEGASSLLMPQTMGYQRAFAML
VMGRTMTAADAHIAGFVNTVVAPGHTEVEAHKVARDIARLPAEAVAIGRKLIRTAPDELTRRIDQEAYLFAERMTSEEAI
GAFKAFLSRKKKK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=253, Percent_Identity=35.1778656126482, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI260275230, Length=253, Percent_Identity=35.1778656126482, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI260274832, Length=253, Percent_Identity=35.1778656126482, Blast_Score=137, Evalue=7e-33,
Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=28.2945736434109, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI221136756, Length=253, Percent_Identity=26.8774703557312, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI221136753, Length=253, Percent_Identity=26.8774703557312, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI221307494, Length=253, Percent_Identity=26.8774703557312, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI4757968, Length=253, Percent_Identity=26.8774703557312, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI49355787, Length=253, Percent_Identity=26.8774703557312, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI25453481, Length=253, Percent_Identity=25.6916996047431, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI51538573, Length=253, Percent_Identity=25.6916996047431, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI116235487, Length=253, Percent_Identity=25.6916996047431, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4757966, Length=186, Percent_Identity=29.5698924731183, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI51538581, Length=186, Percent_Identity=29.5698924731183, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=27.5303643724696, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI68989263, Length=210, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI20127408, Length=186, Percent_Identity=25.2688172043011, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI70995211, Length=179, Percent_Identity=29.608938547486, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=27.3092369477912, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1787660, Length=207, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=29.2134831460674, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082183, Length=244, Percent_Identity=25, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=35.5212355212355, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17549921, Length=229, Percent_Identity=33.1877729257642, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=26.8595041322314, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=227, Percent_Identity=28.1938325991189, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=257, Percent_Identity=24.5136186770428, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=213, Percent_Identity=23.943661971831, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=213, Percent_Identity=23.943661971831, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=213, Percent_Identity=23.943661971831, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=26.9230769230769, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=214, Percent_Identity=24.7663551401869, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24654903, Length=252, Percent_Identity=29.3650793650794, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI20129971, Length=201, Percent_Identity=27.363184079602, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653477, Length=201, Percent_Identity=27.363184079602, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920382, Length=176, Percent_Identity=29.5454545454545, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922422, Length=211, Percent_Identity=21.3270142180095, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653139, Length=245, Percent_Identity=23.6734693877551, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27379; Mature: 27248

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHLIVTDEGETRTIKLRRPEKKNAITQAMYLGMSHAIDTAQNNPLVRCIIITGGSGVF
CCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEE
TAGNDLEDFLRAGDAGSEAARTSAATKFLYSLAHNVKPIIAAVDGVAIGIGTTMLFHCDY
ECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEHHHHHHHHHHHH
VLASTTATFSTPFIHLGLVPEGASSLLMPQTMGYQRAFAMLVMGRTMTAADAHIAGFVNT
HHHHCCCCCCCCEEEEEECCCCCCHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VVAPGHTEVEAHKVARDIARLPAEAVAIGRKLIRTAPDELTRRIDQEAYLFAERMTSEEA
HCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHH
IGAFKAFLSRKKKK
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TDHLIVTDEGETRTIKLRRPEKKNAITQAMYLGMSHAIDTAQNNPLVRCIIITGGSGVF
CCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEE
TAGNDLEDFLRAGDAGSEAARTSAATKFLYSLAHNVKPIIAAVDGVAIGIGTTMLFHCDY
ECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEHHHHHHHHHHHH
VLASTTATFSTPFIHLGLVPEGASSLLMPQTMGYQRAFAMLVMGRTMTAADAHIAGFVNT
HHHHCCCCCCCCEEEEEECCCCCCHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VVAPGHTEVEAHKVARDIARLPAEAVAIGRKLIRTAPDELTRRIDQEAYLFAERMTSEEA
HCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHH
IGAFKAFLSRKKKK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA