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The map label for this gene is livM [H]

Identifier: 146339162

GI number: 146339162

Start: 2255737

End: 2257623

Strand: Direct

Name: livM [H]

Synonym: BRADO2120

Alternate gene names: 146339162

Gene position: 2255737-2257623 (Clockwise)

Preceding gene: 146339161

Following gene: 146339163

Centisome position: 30.25

GC content: 65.92

Gene sequence:

>1887_bases
ATGGCCTTCTATTTCGTCCAGTTCCTGACCGGCCTTGCCAGCGCGGCCTCGCTGTTCCTGGTGGCATCGGGACTGTCGAT
CATCTTCGGCGTCACCCGCATTGTTAATTTCGCGCATGGCGCGTTCTACATGCTCGGCGCCTACGTGGCGTTCTCGCTCA
CCGAGCGGCTGTCGGGCCCGCTGGGCTTCTGGGGCGGCATCGTCGTCGCAGCGCTCGCAGTGGCCGTCATCGGCGTGCTG
GTCGAGATCGTGCTGCTGCGCCGGATCTATCATGCGCCGGAGCTGTTCCAACTCCTCGGCACGTTCGGTCTGACCCTGAT
GGTCGAGGATTTGGTCGTCCTGATTTGGGGGCCGGACGATCTGGTCGGCCGCCGCGCGCCGGGCTTCAAGGGCGCCGTCG
ATTTCTTCGGCCAGAACATTCCGAGCTACGACCTGTTCCTGATCGTGCTCGGCCCCGTCGTGCTCGGGGCGCTGTGGCTG
CTGTTCCAGCGCACGCGCTGGGGCATCCTGGTGCGCGCGGCGACGCAGGACCGCGATATGGTCGCGGCGCTCGGCGTCAA
TCAGAAATGGCTGTTCACCTCGGTGTTCGCTGTCGGCGTCTTCCTCGCCGCGCTCGGCGGCGCGCTGCAGATCCCGCGCG
ATGCCGTGCATCACGCGATGGACCTGCGCATCATCGTCGAGGTGTTCGTTGTCGTCGTGATCGGCGGCCTCGGCAGCATT
TTGGGCGCCTTCGTCGCCGCGGTGCTGGTGTCCGAACTCAACGCCTTCGGCATCCTGATCTTCCCGACCATCTCGCTCAT
CCTGGTGTTCCTGGTGATGGCCGTCGTGCTGGTGGTACGGCCCTGGGGCCTGTTCGGCAGGAAAGAGGCGCCGGCGCGCC
GCACGCCGGGTCTCACCGTCATTCCCTGGCGTCCCCTGAGCTCGGTCGAGCGGCTCGCCTCCCTCGTCGCGCTGGCGTTT
GCCGCGATGCTACCCTTTATCGCCGGCAACTACGCGCTGACCGTCGGCTCTGAGATCGCGATCTTCGTGATCTTCGCGGC
CTCCTTGCATTTCCTGATGGCGGTCGGCGGCCTCGCCTCGTTCGGCCACGCTGCCTATTTCGGCCTCGGCGCCTATGGTG
TGGCGTTCCTCGCCAAGATGGCGGGACTGCCGATGATCGCCTGTCTGCTGCTTGGACCTCTGTTGGGTGCGGCGGGCGCG
GCCGTGTTCGGCGCCTTTGCCGTGCAGCTCTCCGGCGTGTACTTCGCGATGCTGACGCTCGCCTTTGCCCAGATCGTCTG
GTCGATCGCCTTCCAATGGGTCGCCGTGACCGGCGGCGACAATGGCATTCTCGGGCTTTGGCCGGAAAAATGGGCGGCCT
CGCCGTCGCATTTCTACTGGCTGGCGCTCGGTGTTTCCGCATTCGTCGTCAGCGTGCTGCGCCTCATCACGTTCTCGCCG
TTCGGCTATGCCCTGCGCGGCATGCGCGATTCGCCGCTGCGCAGCGAATCGATCGGCATCAACGGCAAGCGCATCCAGTG
GACGGCCTTCATCATCGCAGGCACGACCGCCGGCATCGGCGGCGCGCTGTTCGCGTACCTGAAGGGGAGCGTCTTCCCCG
ACAACATGGGGATCTCGCTGTCGGTCGACGCGCTGGTCATGGTGCTGCTCGGTGGCGTCGAAACCGTGCCCGGCGCGATC
TTCGGCGCCATCGTCTACAAGGCGCTCAACATCTGGCTGGTCAGCCAGACCGACTGGTCGAAGCTCGTGCTCGGCATCTT
CATCGTGCTCATCGTGGTGGTATTCCCGAAGGGCATCGTCGGCGTGGTCGAGAGCGTCATGCACCGGCGGCGCGCGACGG
CGCCCAAGCCTGCCACGCTGTCGGGCAAGATGGAGGGCGCGCAATGA

Upstream 100 bases:

>100_bases
ACAGGTTCAGGCCGAGGACCCTCACCCCACGCTTGCGGCTCTTCACACGATCCGCGCCCCGCTCGTTCAAAATCGACGAT
CTCCCTCACGCGGACCGCCC

Downstream 100 bases:

>100_bases
GTCTCGGCGTATCGCTGCTCTCGGTCGAGGGCCTGTCGAAATCCTACGGCGGCATCCACGCCGTCCGCAACGTGTCGTTC
ATGCTGCAGGCCGGCGAGAT

Product: putative high-affinity branched-chain amino acid transport system permease livM

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MAFYFVQFLTGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAFSLTERLSGPLGFWGGIVVAALAVAVIGVL
VEIVLLRRIYHAPELFQLLGTFGLTLMVEDLVVLIWGPDDLVGRRAPGFKGAVDFFGQNIPSYDLFLIVLGPVVLGALWL
LFQRTRWGILVRAATQDRDMVAALGVNQKWLFTSVFAVGVFLAALGGALQIPRDAVHHAMDLRIIVEVFVVVVIGGLGSI
LGAFVAAVLVSELNAFGILIFPTISLILVFLVMAVVLVVRPWGLFGRKEAPARRTPGLTVIPWRPLSSVERLASLVALAF
AAMLPFIAGNYALTVGSEIAIFVIFAASLHFLMAVGGLASFGHAAYFGLGAYGVAFLAKMAGLPMIACLLLGPLLGAAGA
AVFGAFAVQLSGVYFAMLTLAFAQIVWSIAFQWVAVTGGDNGILGLWPEKWAASPSHFYWLALGVSAFVVSVLRLITFSP
FGYALRGMRDSPLRSESIGINGKRIQWTAFIIAGTTAGIGGALFAYLKGSVFPDNMGISLSVDALVMVLLGGVETVPGAI
FGAIVYKALNIWLVSQTDWSKLVLGIFIVLIVVVFPKGIVGVVESVMHRRRATAPKPATLSGKMEGAQ

Sequences:

>Translated_628_residues
MAFYFVQFLTGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAFSLTERLSGPLGFWGGIVVAALAVAVIGVL
VEIVLLRRIYHAPELFQLLGTFGLTLMVEDLVVLIWGPDDLVGRRAPGFKGAVDFFGQNIPSYDLFLIVLGPVVLGALWL
LFQRTRWGILVRAATQDRDMVAALGVNQKWLFTSVFAVGVFLAALGGALQIPRDAVHHAMDLRIIVEVFVVVVIGGLGSI
LGAFVAAVLVSELNAFGILIFPTISLILVFLVMAVVLVVRPWGLFGRKEAPARRTPGLTVIPWRPLSSVERLASLVALAF
AAMLPFIAGNYALTVGSEIAIFVIFAASLHFLMAVGGLASFGHAAYFGLGAYGVAFLAKMAGLPMIACLLLGPLLGAAGA
AVFGAFAVQLSGVYFAMLTLAFAQIVWSIAFQWVAVTGGDNGILGLWPEKWAASPSHFYWLALGVSAFVVSVLRLITFSP
FGYALRGMRDSPLRSESIGINGKRIQWTAFIIAGTTAGIGGALFAYLKGSVFPDNMGISLSVDALVMVLLGGVETVPGAI
FGAIVYKALNIWLVSQTDWSKLVLGIFIVLIVVVFPKGIVGVVESVMHRRRATAPKPATLSGKMEGAQ
>Mature_627_residues
AFYFVQFLTGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAFSLTERLSGPLGFWGGIVVAALAVAVIGVLV
EIVLLRRIYHAPELFQLLGTFGLTLMVEDLVVLIWGPDDLVGRRAPGFKGAVDFFGQNIPSYDLFLIVLGPVVLGALWLL
FQRTRWGILVRAATQDRDMVAALGVNQKWLFTSVFAVGVFLAALGGALQIPRDAVHHAMDLRIIVEVFVVVVIGGLGSIL
GAFVAAVLVSELNAFGILIFPTISLILVFLVMAVVLVVRPWGLFGRKEAPARRTPGLTVIPWRPLSSVERLASLVALAFA
AMLPFIAGNYALTVGSEIAIFVIFAASLHFLMAVGGLASFGHAAYFGLGAYGVAFLAKMAGLPMIACLLLGPLLGAAGAA
VFGAFAVQLSGVYFAMLTLAFAQIVWSIAFQWVAVTGGDNGILGLWPEKWAASPSHFYWLALGVSAFVVSVLRLITFSPF
GYALRGMRDSPLRSESIGINGKRIQWTAFIIAGTTAGIGGALFAYLKGSVFPDNMGISLSVDALVMVLLGGVETVPGAIF
GAIVYKALNIWLVSQTDWSKLVLGIFIVLIVVVFPKGIVGVVESVMHRRRATAPKPATLSGKMEGAQ

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=318, Percent_Identity=26.7295597484277, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1789866, Length=305, Percent_Identity=25.5737704918033, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 66687; Mature: 66556

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFYFVQFLTGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAFSLTERLSGP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LGFWGGIVVAALAVAVIGVLVEIVLLRRIYHAPELFQLLGTFGLTLMVEDLVVLIWGPDD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCH
LVGRRAPGFKGAVDFFGQNIPSYDLFLIVLGPVVLGALWLLFQRTRWGILVRAATQDRDM
HCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
VAALGVNQKWLFTSVFAVGVFLAALGGALQIPRDAVHHAMDLRIIVEVFVVVVIGGLGSI
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAFVAAVLVSELNAFGILIFPTISLILVFLVMAVVLVVRPWGLFGRKEAPARRTPGLTV
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
IPWRPLSSVERLASLVALAFAAMLPFIAGNYALTVGSEIAIFVIFAASLHFLMAVGGLAS
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
FGHAAYFGLGAYGVAFLAKMAGLPMIACLLLGPLLGAAGAAVFGAFAVQLSGVYFAMLTL
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAQIVWSIAFQWVAVTGGDNGILGLWPEKWAASPSHFYWLALGVSAFVVSVLRLITFSP
HHHHHHHHHHHHEEEEECCCCCEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
FGYALRGMRDSPLRSESIGINGKRIQWTAFIIAGTTAGIGGALFAYLKGSVFPDNMGISL
HHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEE
SVDALVMVLLGGVETVPGAIFGAIVYKALNIWLVSQTDWSKLVLGIFIVLIVVVFPKGIV
EHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCHHH
GVVESVMHRRRATAPKPATLSGKMEGAQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AFYFVQFLTGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAFSLTERLSGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LGFWGGIVVAALAVAVIGVLVEIVLLRRIYHAPELFQLLGTFGLTLMVEDLVVLIWGPDD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCH
LVGRRAPGFKGAVDFFGQNIPSYDLFLIVLGPVVLGALWLLFQRTRWGILVRAATQDRDM
HCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
VAALGVNQKWLFTSVFAVGVFLAALGGALQIPRDAVHHAMDLRIIVEVFVVVVIGGLGSI
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAFVAAVLVSELNAFGILIFPTISLILVFLVMAVVLVVRPWGLFGRKEAPARRTPGLTV
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
IPWRPLSSVERLASLVALAFAAMLPFIAGNYALTVGSEIAIFVIFAASLHFLMAVGGLAS
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
FGHAAYFGLGAYGVAFLAKMAGLPMIACLLLGPLLGAAGAAVFGAFAVQLSGVYFAMLTL
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAQIVWSIAFQWVAVTGGDNGILGLWPEKWAASPSHFYWLALGVSAFVVSVLRLITFSP
HHHHHHHHHHHHEEEEECCCCCEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
FGYALRGMRDSPLRSESIGINGKRIQWTAFIIAGTTAGIGGALFAYLKGSVFPDNMGISL
HHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEE
SVDALVMVLLGGVETVPGAIFGAIVYKALNIWLVSQTDWSKLVLGIFIVLIVVVFPKGIV
EHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCHHH
GVVESVMHRRRATAPKPATLSGKMEGAQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]