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The map label for this gene is rfbE [H]

Identifier: 146339114

GI number: 146339114

Start: 2205346

End: 2206407

Strand: Reverse

Name: rfbE [H]

Synonym: BRADO2068

Alternate gene names: 146339114

Gene position: 2206407-2205346 (Counterclockwise)

Preceding gene: 146339115

Following gene: 146339113

Centisome position: 29.59

GC content: 64.88

Gene sequence:

>1062_bases
ATGATGGAAGCGACCTCGACTCAGGCTCCGATCGTGATCACCGGGGGCTGCGGCTTCATCGGCTGCAACCTTGCCGACCG
GCTCGCGACGCGTGGCGACCATGTGCTGATCCTCGACAACCTCGCGCGCGCCGGGGTGCGTGAAAATGCGCAATGGCTGA
AGTCGCGCCATGGCGATCGGATCACCATCACGGTCGGCGACATCCGCGAGCCGATCACGGTAATCGACACCGTGAAACAA
GCCAAGGCCGTGCTGCATCTCGCGGCACAGGTTGCGGTGACTTCCAGCCTCGACAACCCCGTCGACGATTTCGAGATCAA
TGCACGCGGCACTTTGAACGTGCTGGAAGCCGTTCGCTTGCACAATGCAGCCGCGCCCGTGCTGTTCGCCTCGACCAACA
AAGTGTACGGACGACTGATCGACGACGACGCGATCGCCCGCATTGGTCAGCGCTATCAGCCAATCAGCACGACGCTGACC
CAAGGCGTCTCCGAGCAGACGTCGCTCGATCTCTACAGCCCCTACGGCTGCTCCAAGGGCGCGGCCGATCAGTACGTTCA
CGACTATGCCCGCGTCTACGGCCTGAACACGGCGGTTCTGCGGATGAGCTGCATCTATGGTCCGCGGCAGTTCGGCAATG
AGGATCAGGGCTGGATCGCGCACTTCGTTCTCTCGGCCCTGCGCGGCGCGGCGTTGACGATCTATGGCGACGGCTGTCAG
GTGCGCGACGCGCTGTACGTCGCCGATGCGGTCGACGCCTGGCTCGCGGTGCTCGATCAGATCGAGGCGACGCGCGGGCG
CGTGTTCAACCTCGGCGGTGGCCCGTCGAACGCCATCAGTCTGCTGGAGCTGATCGACCAGATCGGGCAACTTTCCGGGC
CGGTCAATTACAGCTTCTCGGATTGGCGCCCCGGCGACCAGCCCTGGTACGTCACCGACATCGGCGCACTCGCGGGCGCG
ACCGGATGGCGGCCGCGGACATCCTTCGTCGAGGGACTTCGCGAGTTGCATGGCTGGCTGGCAACCCGCTTCGCCGAACG
CGGCCAGGGGGCGCTGGCATGA

Upstream 100 bases:

>100_bases
AGGATGGGCTGGACGAGCTGGCACATTACCTCGCCGACCAGATCGCCGACGACCAGGCGGAGAAGGCGACCGAAGAGCTG
TCGCAACGCGGGCTGGTTGC

Downstream 100 bases:

>100_bases
CCCGCGTCGCCCTGATCAATCCGAACTGGACGTTCGACGGCAGCATCTATTTCGGCTGCCGCAGCCCGCATCTGCCGATC
GAGCTCGGCATTTCCGAGCA

Product: putative CDP-tyvelose-2-epimerase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MMEATSTQAPIVITGGCGFIGCNLADRLATRGDHVLILDNLARAGVRENAQWLKSRHGDRITITVGDIREPITVIDTVKQ
AKAVLHLAAQVAVTSSLDNPVDDFEINARGTLNVLEAVRLHNAAAPVLFASTNKVYGRLIDDDAIARIGQRYQPISTTLT
QGVSEQTSLDLYSPYGCSKGAADQYVHDYARVYGLNTAVLRMSCIYGPRQFGNEDQGWIAHFVLSALRGAALTIYGDGCQ
VRDALYVADAVDAWLAVLDQIEATRGRVFNLGGGPSNAISLLELIDQIGQLSGPVNYSFSDWRPGDQPWYVTDIGALAGA
TGWRPRTSFVEGLRELHGWLATRFAERGQGALA

Sequences:

>Translated_353_residues
MMEATSTQAPIVITGGCGFIGCNLADRLATRGDHVLILDNLARAGVRENAQWLKSRHGDRITITVGDIREPITVIDTVKQ
AKAVLHLAAQVAVTSSLDNPVDDFEINARGTLNVLEAVRLHNAAAPVLFASTNKVYGRLIDDDAIARIGQRYQPISTTLT
QGVSEQTSLDLYSPYGCSKGAADQYVHDYARVYGLNTAVLRMSCIYGPRQFGNEDQGWIAHFVLSALRGAALTIYGDGCQ
VRDALYVADAVDAWLAVLDQIEATRGRVFNLGGGPSNAISLLELIDQIGQLSGPVNYSFSDWRPGDQPWYVTDIGALAGA
TGWRPRTSFVEGLRELHGWLATRFAERGQGALA
>Mature_353_residues
MMEATSTQAPIVITGGCGFIGCNLADRLATRGDHVLILDNLARAGVRENAQWLKSRHGDRITITVGDIREPITVIDTVKQ
AKAVLHLAAQVAVTSSLDNPVDDFEINARGTLNVLEAVRLHNAAAPVLFASTNKVYGRLIDDDAIARIGQRYQPISTTLT
QGVSEQTSLDLYSPYGCSKGAADQYVHDYARVYGLNTAVLRMSCIYGPRQFGNEDQGWIAHFVLSALRGAALTIYGDGCQ
VRDALYVADAVDAWLAVLDQIEATRGRVFNLGGGPSNAISLLELIDQIGQLSGPVNYSFSDWRPGDQPWYVTDIGALAGA
TGWRPRTSFVEGLRELHGWLATRFAERGQGALA

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=333, Percent_Identity=27.9279279279279, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI7657641, Length=344, Percent_Identity=24.7093023255814, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI56237023, Length=322, Percent_Identity=24.5341614906832, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI56118217, Length=322, Percent_Identity=24.5341614906832, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI189083684, Length=322, Percent_Identity=24.5341614906832, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=31.700288184438, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI48994969, Length=357, Percent_Identity=31.6526610644258, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786974, Length=348, Percent_Identity=27.2988505747126, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=28.4023668639053, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17568069, Length=337, Percent_Identity=27.0029673590504, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=26.0479041916168, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356223, Length=339, Percent_Identity=27.1386430678466, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 38174; Mature: 38174

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEATSTQAPIVITGGCGFIGCNLADRLATRGDHVLILDNLARAGVRENAQWLKSRHGDR
CCCCCCCCCCEEEECCCCEEECCHHHHHHCCCCEEEEEECHHHCCHHHHHHHHHHCCCCE
ITITVGDIREPITVIDTVKQAKAVLHLAAQVAVTSSLDNPVDDFEINARGTLNVLEAVRL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHH
HNAAAPVLFASTNKVYGRLIDDDAIARIGQRYQPISTTLTQGVSEQTSLDLYSPYGCSKG
HCCCCCEEEECCCHHHEEECCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
AADQYVHDYARVYGLNTAVLRMSCIYGPRQFGNEDQGWIAHFVLSALRGAALTIYGDGCQ
CHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCC
VRDALYVADAVDAWLAVLDQIEATRGRVFNLGGGPSNAISLLELIDQIGQLSGPVNYSFS
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
DWRPGDQPWYVTDIGALAGATGWRPRTSFVEGLRELHGWLATRFAERGQGALA
CCCCCCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMEATSTQAPIVITGGCGFIGCNLADRLATRGDHVLILDNLARAGVRENAQWLKSRHGDR
CCCCCCCCCCEEEECCCCEEECCHHHHHHCCCCEEEEEECHHHCCHHHHHHHHHHCCCCE
ITITVGDIREPITVIDTVKQAKAVLHLAAQVAVTSSLDNPVDDFEINARGTLNVLEAVRL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHH
HNAAAPVLFASTNKVYGRLIDDDAIARIGQRYQPISTTLTQGVSEQTSLDLYSPYGCSKG
HCCCCCEEEECCCHHHEEECCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
AADQYVHDYARVYGLNTAVLRMSCIYGPRQFGNEDQGWIAHFVLSALRGAALTIYGDGCQ
CHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCC
VRDALYVADAVDAWLAVLDQIEATRGRVFNLGGGPSNAISLLELIDQIGQLSGPVNYSFS
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
DWRPGDQPWYVTDIGALAGATGWRPRTSFVEGLRELHGWLATRFAERGQGALA
CCCCCCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]