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The map label for this gene is 146338845

Identifier: 146338845

GI number: 146338845

Start: 1902950

End: 1903948

Strand: Direct

Name: 146338845

Synonym: BRADO1782

Alternate gene names: NA

Gene position: 1902950-1903948 (Clockwise)

Preceding gene: 146338844

Following gene: 146338846

Centisome position: 25.52

GC content: 66.67

Gene sequence:

>999_bases
ATGGGCCCGACGCAACGGGCCGACACCCGCGCGCACACGCTCGCGCGGATGATTGCGCTGCTGGAGGGCGCGGCCGCGCA
GGGCGCGACGCTGGTGGTGTTCCCGGAACTCGCCTTCACCACGTTCTTTCCGCGCTGGATCCTGGAGCGCGATGCGCTCG
ACAGCTATTTCGAGCGGTCGATGCCCAACCCTTCCGTGGCTGCGCTGTTCGATCGGGCGCGCGAACTGCGCGTCGGCTTC
TATGTCGGCTACGCCGAGCTGACGCCGGACGGTCGGCGTTTCAACAGCGCCATCCTGGTCGATGCCGACGGCCAGCTCAT
CAGCAAGTACCGCAAGGTCCATCTGCCGGGCTCGGTCGAGCCGCGCGAAGGAGCCAGATATCAGCAGCTCGAGAAGCGCT
ATTTCGGCTATGGCGATCTCGGCTTTCCGGCGGTGCGCGCCGGTCCCGAATGGGGCGGTGCGATCATGGGCATGATGATC
TGCAACGACAGGCGCTGGCCGGAATCCTGGCGCATGCTCGGGATGCAAGGCGTTGAGCTGGTCTGCGTCGGCTACAACTC
CGCGGCCTATGATCCGAACGGCGGCAACACCGAGGATGCGTCGTTGCGCACGTTCCACTCGACGCTGGTGGCGCAGGCCA
ATGCCTATATGAACGCGACCTGGGCAATCGCCGTGGCCAAGGCGGGCGACGAGGACGGCAGCGGACTGATCGGCGGCTCC
TGCATCGTCGATCCCAATGGCTGCATCGTCGCGCAGGCGACGACGCTCGCGGACGAGGTCCTGGTCGCCGACATCGATCT
CGATGCGTGCCGGCAGGGCAAGGACAAGATGTTCAACTTCGCTGCGCATCGCCGCCCCGATCAGTACAAGGTCATCACCG
AGCGCGCCGGCGTCGTCGAGCCGGTGCCGGTGGATAGCGGCCTGCGCTGCACGAAGCCAGGCTGCGGGCCCGGATTGCGC
GGCGACGTCTTCGCCACAGCGATTGCGACCTCTCGATGA

Upstream 100 bases:

>100_bases
CCTGTCGCGCGCTGGACGGCCGGCAATGGCGCCGGTGATGCCGGTCGCGCGCAGATTGGAGATCGCCGTGAGTCGTATCT
TTCGCGCCGCCGCTGCCCAG

Downstream 100 bases:

>100_bases
AGGATTCGGGCGCGCGTTCACACGGCGCCGTGGTTCCTCTCATTCCACACTGTCAAACAGCTGGGCAAGCCGCATCGTTG
CCGCGGCGCGCAGGCGCCCG

Product: N-carbamoyl-D-amino acid hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MGPTQRADTRAHTLARMIALLEGAAAQGATLVVFPELAFTTFFPRWILERDALDSYFERSMPNPSVAALFDRARELRVGF
YVGYAELTPDGRRFNSAILVDADGQLISKYRKVHLPGSVEPREGARYQQLEKRYFGYGDLGFPAVRAGPEWGGAIMGMMI
CNDRRWPESWRMLGMQGVELVCVGYNSAAYDPNGGNTEDASLRTFHSTLVAQANAYMNATWAIAVAKAGDEDGSGLIGGS
CIVDPNGCIVAQATTLADEVLVADIDLDACRQGKDKMFNFAAHRRPDQYKVITERAGVVEPVPVDSGLRCTKPGCGPGLR
GDVFATAIATSR

Sequences:

>Translated_332_residues
MGPTQRADTRAHTLARMIALLEGAAAQGATLVVFPELAFTTFFPRWILERDALDSYFERSMPNPSVAALFDRARELRVGF
YVGYAELTPDGRRFNSAILVDADGQLISKYRKVHLPGSVEPREGARYQQLEKRYFGYGDLGFPAVRAGPEWGGAIMGMMI
CNDRRWPESWRMLGMQGVELVCVGYNSAAYDPNGGNTEDASLRTFHSTLVAQANAYMNATWAIAVAKAGDEDGSGLIGGS
CIVDPNGCIVAQATTLADEVLVADIDLDACRQGKDKMFNFAAHRRPDQYKVITERAGVVEPVPVDSGLRCTKPGCGPGLR
GDVFATAIATSR
>Mature_331_residues
GPTQRADTRAHTLARMIALLEGAAAQGATLVVFPELAFTTFFPRWILERDALDSYFERSMPNPSVAALFDRARELRVGFY
VGYAELTPDGRRFNSAILVDADGQLISKYRKVHLPGSVEPREGARYQQLEKRYFGYGDLGFPAVRAGPEWGGAIMGMMIC
NDRRWPESWRMLGMQGVELVCVGYNSAAYDPNGGNTEDASLRTFHSTLVAQANAYMNATWAIAVAKAGDEDGSGLIGGSC
IVDPNGCIVAQATTLADEVLVADIDLDACRQGKDKMFNFAAHRRPDQYKVITERAGVVEPVPVDSGLRCTKPGCGPGLRG
DVFATAIATSR

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 36069; Mature: 35938

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPTQRADTRAHTLARMIALLEGAAAQGATLVVFPELAFTTFFPRWILERDALDSYFERS
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHC
MPNPSVAALFDRARELRVGFYVGYAELTPDGRRFNSAILVDADGQLISKYRKVHLPGSVE
CCCCHHHHHHHHHHHEEEEEEEEEEEECCCCCEECCEEEEECCCHHHHHHHEEECCCCCC
PREGARYQQLEKRYFGYGDLGFPAVRAGPEWGGAIMGMMICNDRRWPESWRMLGMQGVEL
CCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCHHEEEEEEECCCCCCHHHHHHCCCCCEE
VCVGYNSAAYDPNGGNTEDASLRTFHSTLVAQANAYMNATWAIAVAKAGDEDGSGLIGGS
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEECCE
CIVDPNGCIVAQATTLADEVLVADIDLDACRQGKDKMFNFAAHRRPDQYKVITERAGVVE
EEECCCCEEEEEHHHHHCEEEEEECCHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCC
PVPVDSGLRCTKPGCGPGLRGDVFATAIATSR
CCCCCCCCEECCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
GPTQRADTRAHTLARMIALLEGAAAQGATLVVFPELAFTTFFPRWILERDALDSYFERS
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHC
MPNPSVAALFDRARELRVGFYVGYAELTPDGRRFNSAILVDADGQLISKYRKVHLPGSVE
CCCCHHHHHHHHHHHEEEEEEEEEEEECCCCCEECCEEEEECCCHHHHHHHEEECCCCCC
PREGARYQQLEKRYFGYGDLGFPAVRAGPEWGGAIMGMMICNDRRWPESWRMLGMQGVEL
CCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCHHEEEEEEECCCCCCHHHHHHCCCCCEE
VCVGYNSAAYDPNGGNTEDASLRTFHSTLVAQANAYMNATWAIAVAKAGDEDGSGLIGGS
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEECCE
CIVDPNGCIVAQATTLADEVLVADIDLDACRQGKDKMFNFAAHRRPDQYKVITERAGVVE
EEECCCCEEEEEHHHHHCEEEEEECCHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCC
PVPVDSGLRCTKPGCGPGLRGDVFATAIATSR
CCCCCCCCEECCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]