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The map label for this gene is hypF [H]

Identifier: 146338763

GI number: 146338763

Start: 1811030

End: 1813288

Strand: Direct

Name: hypF [H]

Synonym: BRADO1697

Alternate gene names: 146338763

Gene position: 1811030-1813288 (Clockwise)

Preceding gene: 146338762

Following gene: 146338764

Centisome position: 24.29

GC content: 67.33

Gene sequence:

>2259_bases
ATGAGCACGGCGGGCCCGACATTTGCAGCGGATCGCGCGCGTCTCAGGCTCCGCGTGCGCGGTGCCGTGCAGGGCGTGGG
CTTCCGTCCCTATGTCCATGGCCTGGCCACACGCTATCGCCTTGGCGGCTTCGTCGCCAACGACGCTGATGGTGTCGTCA
TCGAGGTCGAGGGCGAGAGTGTCATGTCGTTTGTCGAAGCGCTGCCGCGACAGGCGCCGCCTCTGGCGCGTATCGACGAC
ATCGCCGTGGAACCTGTCGCCGCGCAGGCGAGTGCAGACTTCCGCATCGGCGAGAGCGTGGGCGGCCGGGTGACCACCCG
CATCGGTCCGGATGCGGCAACCTGTCCGGCCTGCCTTGCCGAGTTGTTCGATCCGGGCAGTCGATTTCATCGCTATCCTT
TCGTCAACTGCACCCATTGCGGCCCGCGCTTCACCATCGCCGAGCGCCTGCCTTACGACCGCGCGACGACGGCAATGAAG
AGCTTCCCGATGTGCGAGGCCTGCCGCTCGGACTATGAGGATCCAAGCGGCCGCCGCTTCCATGCCGAGGCGATCTCCTG
TCCGGATTGCGGGCCCCGGCTCAGCCACGACGTCGGTGAGATCGCGGCGGCGCTTGCGGAGGGAAAGATTGTCGCGATCA
AGGGACTCGGCGGCTATCAGATGCTGTGTGATGCGCGCGATGAGCCTGCGGTGCAGCGGCTGCGCGCGCGCAAGCAGCGC
GACGGCAAGCCGTTCGCTGTGATGCTGGCCTCGGTCGAAGCCGCTGGGGAAATTGCCGATCTCGATGCCGCCGAGCGCGG
ATTGCTGGAGCAGGTGGCCCGGCCGATCGTTCTGCTGCGCTCGCGCGGTCGTCTGGCGCCCTCGGTAGCGCCCGGTCTCG
CCAAGATCGGCGTGATGCTCCCGGTCGCGCCGCTGCATCATCTGGTCTTTGACGCGCTGCGTGCGCATGGTGGCAAACGG
AACGAGGCGCTGGTCTGCACCAGCGCCAATCCCAGCGGTCAGCCGCTGCTGACCGACAACGACGAGGCTCTGATCGATCT
CGCCGGCATCGCGGATCTGATCGTCACGCATGACCGCGACATTGTCATTCGTGCCGATGATTCCGTTGTCGCCATGATGG
CGGACTCCCCACGCTTCATCCGTCGGGCCCGCGGCTACGTTCCGGAACCGATCCGGCTGCCGCGCGCTCTCCCGCCGCTC
CTCGCGGTCGGGGCTCACCTCAAGTCGACGGTGACGGTGATCCGTGGCGACGAGGCCTTCGTGTCGCAGCATCTCGGCGA
CCTCGACACGGCCGCAGCGATCCGCTTCTTCGAGGAGACGATCGCGCATCTCACCTCCATCCTCGACGTCGCGCCGCTGG
CGATCGCGCACGACCTGCATCCGGACATGGCGTCGACGCGGTTCGCGCAGGCTCGGGGACTGCCGACATTCGGCGTGCAG
CATCATCATGCGCATGCCGCCGCGGTCATCGCGGAGCATGGCTTGCGCGCGCCTGTGCTTGCACTCGTGCTTGATGGCTA
TGGCTACGGCACCGATGGCGACGCCTGGGGCGGCGAACTGCTGCTGTGTGACGGAGCGATGTTCCGGCGCCTCGGGCATC
TCGCGCCGCTGAAGATGCCGGGCGGCGACCGCGCCGCGCGGGAACCGTGGCGCATGGCGGCCGCCGTGCTCCACGACCTC
GGACATGACGCCGAGATCGCTGCACGCTTCAGGAGTCAGCCGCAGGCGCGCCATCTGCCGACGTTGCTCGATCGCACCGA
TGCGGCCGTGACGACCAGCGCAGGCCGGCTGTTCGATGCTGTGGCGGGATTGCTCGGGGTCAGCACGGTTCAGAGCTATG
AAGGCGAAGCTGCCATGAAGCTCGAGGCGCTGGTTCGCACCACTGCAGTCCTCGAACAGGGCTGGACGATCGCCGATGGC
ATTCTTTCGCTTCGTCCGCTCCTCGCTCAACTGCTCGCCTGCGACTTGGACCCCGCTGAAGCCGCCGGCCTATTCCACGG
CACACTCGCTGCCGCCTGCGTGGACTGGATCGTATCTGCGTCGCGCGCAACCGGCGTGACCATGCTCGTGCTGAGTGGCG
GCTGCTTCCTCAATGCGCATCTGGCGGAGCTGATCGTCCGCGGCTGCAGAGCCGCCGGCGTCGATGCGTGGCTGCCGCGC
CGGCTGCCGCCCAACGATGGCGGACTGAGTTTAGGGCAGGCCTGGGTCGCCGGGCTCCAATTGATCAAGCAAGACAACGC
AGCTGGAGGGATCGTCTGA

Upstream 100 bases:

>100_bases
CAATCTCGGCCAAGACCGGCGAAGGTTTTTCCGCCTTCTATGCCTGGATCAGAAAGCAGGCCGACCGCGTCAGAGCGAGC
GCGCTGGGCGCGGTGCAGGG

Downstream 100 bases:

>100_bases
TGTGCCTGGCTATTCCTGCCGAGGTGATCAAGCTGCTACCCGACGACATGGCGATCGTGTCGATCGACGGTGTCAGCAAG
GAGGTCTCGGTCGCGCTGAT

Product: carbamoyl phosphate phosphatase

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 752; Mature: 751

Protein sequence:

>752_residues
MSTAGPTFAADRARLRLRVRGAVQGVGFRPYVHGLATRYRLGGFVANDADGVVIEVEGESVMSFVEALPRQAPPLARIDD
IAVEPVAAQASADFRIGESVGGRVTTRIGPDAATCPACLAELFDPGSRFHRYPFVNCTHCGPRFTIAERLPYDRATTAMK
SFPMCEACRSDYEDPSGRRFHAEAISCPDCGPRLSHDVGEIAAALAEGKIVAIKGLGGYQMLCDARDEPAVQRLRARKQR
DGKPFAVMLASVEAAGEIADLDAAERGLLEQVARPIVLLRSRGRLAPSVAPGLAKIGVMLPVAPLHHLVFDALRAHGGKR
NEALVCTSANPSGQPLLTDNDEALIDLAGIADLIVTHDRDIVIRADDSVVAMMADSPRFIRRARGYVPEPIRLPRALPPL
LAVGAHLKSTVTVIRGDEAFVSQHLGDLDTAAAIRFFEETIAHLTSILDVAPLAIAHDLHPDMASTRFAQARGLPTFGVQ
HHHAHAAAVIAEHGLRAPVLALVLDGYGYGTDGDAWGGELLLCDGAMFRRLGHLAPLKMPGGDRAAREPWRMAAAVLHDL
GHDAEIAARFRSQPQARHLPTLLDRTDAAVTTSAGRLFDAVAGLLGVSTVQSYEGEAAMKLEALVRTTAVLEQGWTIADG
ILSLRPLLAQLLACDLDPAEAAGLFHGTLAAACVDWIVSASRATGVTMLVLSGGCFLNAHLAELIVRGCRAAGVDAWLPR
RLPPNDGGLSLGQAWVAGLQLIKQDNAAGGIV

Sequences:

>Translated_752_residues
MSTAGPTFAADRARLRLRVRGAVQGVGFRPYVHGLATRYRLGGFVANDADGVVIEVEGESVMSFVEALPRQAPPLARIDD
IAVEPVAAQASADFRIGESVGGRVTTRIGPDAATCPACLAELFDPGSRFHRYPFVNCTHCGPRFTIAERLPYDRATTAMK
SFPMCEACRSDYEDPSGRRFHAEAISCPDCGPRLSHDVGEIAAALAEGKIVAIKGLGGYQMLCDARDEPAVQRLRARKQR
DGKPFAVMLASVEAAGEIADLDAAERGLLEQVARPIVLLRSRGRLAPSVAPGLAKIGVMLPVAPLHHLVFDALRAHGGKR
NEALVCTSANPSGQPLLTDNDEALIDLAGIADLIVTHDRDIVIRADDSVVAMMADSPRFIRRARGYVPEPIRLPRALPPL
LAVGAHLKSTVTVIRGDEAFVSQHLGDLDTAAAIRFFEETIAHLTSILDVAPLAIAHDLHPDMASTRFAQARGLPTFGVQ
HHHAHAAAVIAEHGLRAPVLALVLDGYGYGTDGDAWGGELLLCDGAMFRRLGHLAPLKMPGGDRAAREPWRMAAAVLHDL
GHDAEIAARFRSQPQARHLPTLLDRTDAAVTTSAGRLFDAVAGLLGVSTVQSYEGEAAMKLEALVRTTAVLEQGWTIADG
ILSLRPLLAQLLACDLDPAEAAGLFHGTLAAACVDWIVSASRATGVTMLVLSGGCFLNAHLAELIVRGCRAAGVDAWLPR
RLPPNDGGLSLGQAWVAGLQLIKQDNAAGGIV
>Mature_751_residues
STAGPTFAADRARLRLRVRGAVQGVGFRPYVHGLATRYRLGGFVANDADGVVIEVEGESVMSFVEALPRQAPPLARIDDI
AVEPVAAQASADFRIGESVGGRVTTRIGPDAATCPACLAELFDPGSRFHRYPFVNCTHCGPRFTIAERLPYDRATTAMKS
FPMCEACRSDYEDPSGRRFHAEAISCPDCGPRLSHDVGEIAAALAEGKIVAIKGLGGYQMLCDARDEPAVQRLRARKQRD
GKPFAVMLASVEAAGEIADLDAAERGLLEQVARPIVLLRSRGRLAPSVAPGLAKIGVMLPVAPLHHLVFDALRAHGGKRN
EALVCTSANPSGQPLLTDNDEALIDLAGIADLIVTHDRDIVIRADDSVVAMMADSPRFIRRARGYVPEPIRLPRALPPLL
AVGAHLKSTVTVIRGDEAFVSQHLGDLDTAAAIRFFEETIAHLTSILDVAPLAIAHDLHPDMASTRFAQARGLPTFGVQH
HHAHAAAVIAEHGLRAPVLALVLDGYGYGTDGDAWGGELLLCDGAMFRRLGHLAPLKMPGGDRAAREPWRMAAAVLHDLG
HDAEIAARFRSQPQARHLPTLLDRTDAAVTTSAGRLFDAVAGLLGVSTVQSYEGEAAMKLEALVRTTAVLEQGWTIADGI
LSLRPLLAQLLACDLDPAEAAGLFHGTLAAACVDWIVSASRATGVTMLVLSGGCFLNAHLAELIVRGCRAAGVDAWLPRR
LPPNDGGLSLGQAWVAGLQLIKQDNAAGGIV

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=765, Percent_Identity=43.1372549019608, Blast_Score=471, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 79861; Mature: 79729

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAGPTFAADRARLRLRVRGAVQGVGFRPYVHGLATRYRLGGFVANDADGVVIEVEGES
CCCCCCCCHHHHHHEEEEEEEHHCCCCCCHHHHHHHHHHHHCCEEECCCCCEEEEECCHH
VMSFVEALPRQAPPLARIDDIAVEPVAAQASADFRIGESVGGRVTTRIGPDAATCPACLA
HHHHHHHHCCCCCCCCCHHHHHCCCCHHCCCCCCEECCCCCCEEEEEECCCCHHHHHHHH
ELFDPGSRFHRYPFVNCTHCGPRFTIAERLPYDRATTAMKSFPMCEACRSDYEDPSGRRF
HHCCCCHHHCCCCCCEECCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHCCCCCCCCCEE
HAEAISCPDCGPRLSHDVGEIAAALAEGKIVAIKGLGGYQMLCDARDEPAVQRLRARKQR
EECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHC
DGKPFAVMLASVEAAGEIADLDAAERGLLEQVARPIVLLRSRGRLAPSVAPGLAKIGVML
CCCCEEEEEEHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
PVAPLHHLVFDALRAHGGKRNEALVCTSANPSGQPLLTDNDEALIDLAGIADLIVTHDRD
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEECCCCCEEEHHHHHHHHEECCCC
IVIRADDSVVAMMADSPRFIRRARGYVPEPIRLPRALPPLLAVGAHLKSTVTVIRGDEAF
EEEECCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHEEEEEECCHHH
VSQHLGDLDTAAAIRFFEETIAHLTSILDVAPLAIAHDLHPDMASTRFAQARGLPTFGVQ
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
HHHAHAAAVIAEHGLRAPVLALVLDGYGYGTDGDAWGGELLLCDGAMFRRLGHLAPLKMP
HHHHHHHHHHHHCCCCHHHHHHEECCCCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCCC
GGDRAAREPWRMAAAVLHDLGHDAEIAARFRSQPQARHLPTLLDRTDAAVTTSAGRLFDA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHCCHHHHCCCCCEECCHHHHHHH
VAGLLGVSTVQSYEGEAAMKLEALVRTTAVLEQGWTIADGILSLRPLLAQLLACDLDPAE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHH
AAGLFHGTLAAACVDWIVSASRATGVTMLVLSGGCFLNAHLAELIVRGCRAAGVDAWLPR
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEHHHHHHHHHHHHHHCCCCHHCCC
RLPPNDGGLSLGQAWVAGLQLIKQDNAAGGIV
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STAGPTFAADRARLRLRVRGAVQGVGFRPYVHGLATRYRLGGFVANDADGVVIEVEGES
CCCCCCCHHHHHHEEEEEEEHHCCCCCCHHHHHHHHHHHHCCEEECCCCCEEEEECCHH
VMSFVEALPRQAPPLARIDDIAVEPVAAQASADFRIGESVGGRVTTRIGPDAATCPACLA
HHHHHHHHCCCCCCCCCHHHHHCCCCHHCCCCCCEECCCCCCEEEEEECCCCHHHHHHHH
ELFDPGSRFHRYPFVNCTHCGPRFTIAERLPYDRATTAMKSFPMCEACRSDYEDPSGRRF
HHCCCCHHHCCCCCCEECCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHCCCCCCCCCEE
HAEAISCPDCGPRLSHDVGEIAAALAEGKIVAIKGLGGYQMLCDARDEPAVQRLRARKQR
EECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHC
DGKPFAVMLASVEAAGEIADLDAAERGLLEQVARPIVLLRSRGRLAPSVAPGLAKIGVML
CCCCEEEEEEHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
PVAPLHHLVFDALRAHGGKRNEALVCTSANPSGQPLLTDNDEALIDLAGIADLIVTHDRD
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEECCCCCEEEHHHHHHHHEECCCC
IVIRADDSVVAMMADSPRFIRRARGYVPEPIRLPRALPPLLAVGAHLKSTVTVIRGDEAF
EEEECCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHEEEEEECCHHH
VSQHLGDLDTAAAIRFFEETIAHLTSILDVAPLAIAHDLHPDMASTRFAQARGLPTFGVQ
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
HHHAHAAAVIAEHGLRAPVLALVLDGYGYGTDGDAWGGELLLCDGAMFRRLGHLAPLKMP
HHHHHHHHHHHHCCCCHHHHHHEECCCCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCCC
GGDRAAREPWRMAAAVLHDLGHDAEIAARFRSQPQARHLPTLLDRTDAAVTTSAGRLFDA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHCCHHHHCCCCCEECCHHHHHHH
VAGLLGVSTVQSYEGEAAMKLEALVRTTAVLEQGWTIADGILSLRPLLAQLLACDLDPAE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHH
AAGLFHGTLAAACVDWIVSASRATGVTMLVLSGGCFLNAHLAELIVRGCRAAGVDAWLPR
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEHHHHHHHHHHHHHHCCCCHHCCC
RLPPNDGGLSLGQAWVAGLQLIKQDNAAGGIV
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8326860 [H]