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The map label for this gene is ycaL [H]

Identifier: 146338530

GI number: 146338530

Start: 1554318

End: 1555451

Strand: Direct

Name: ycaL [H]

Synonym: BRADO1449

Alternate gene names: 146338530

Gene position: 1554318-1555451 (Clockwise)

Preceding gene: 146338529

Following gene: 146338533

Centisome position: 20.84

GC content: 68.17

Gene sequence:

>1134_bases
ATGGACTCGACGTCGCCGGATTCTGATTCCGAGCCCGGCGCGGCCGAGGCGACGCGGGCGGTGCAGGTCGCCCCGCCCGG
CGGTCCCGCGACCTATTTCGACGGTTTGTCGAACCGGCGGCAGAACGTGACGATCGGATTTGCCAATCAACTCGTGATGC
GCAGCCCCGACAATTTCGTGTCGTGGCCGTATGCCGATATCCGCCGGGTCGACGGCCCGAACGGCACGCTGCGTGTGAGT
TGTCTGTCGGCGTCACCGCTGGCCCGGCTGGAGATCCGCGACGCGGCGCTGGTGGCCGAGCTGATCGCGCGCTGCAGCCA
GATCGACCAGAACCGGCTGACGCGCGGCGGCATCGCGCGAATCATCGCCTGGTCGTTGGCGGCGGTCGCCTCGATCGTGA
CGCTCGTGCTGGTCGTGATTCCGTTCGCGGCCGATCGGCTCACGCCGCTGGTGCCGCCTGCGATGGAGCGGCATCTCGGC
GAGGCCGCGGTCACGCAAATCAAGACGATCTACCATGCCCAGCTCTGCAGCGAGACGGCCGGTCAGGCGGCGTTCGCCAA
GCTGGTGACCGGACTGCGCCGTCCCGCGGGTCTCGACGACAGCATCGGCTCGCAGGTGCTCGATACGCCTGTTCCCAACG
CCTTCGCCCTGCCGGGCGGGCAGTTCTTCCTGTTCAGGGGGCTGCTGGACACGGCCAATGACCCCGACGAGGTCGCGGGC
GTGCTGGCGCATGAGCTGGGCCACCTCAAGCACCGCGATAATCTGCGGCAGTTGATTCATAACGGCGGCAGCTCCTTCCT
GATCGGTCTGCTGTTCGGCGACGTTACCGGCGCGGGCGCTGCCATCTTTGCCTCCCGCGCCATGATCAACGCGTCCTATT
CGCGCGAGGCGGAGGAGGCGGCCGACAGCTTCGCGATCGAGGTCATGCACAAGCTCGGGCGGCCGACGCGGCCGATGGGC
GAATTGTTGTTTCGCATCACCGGCAAGGAGGCCGGCAAGCAGCCCGCGCTGTGGGCGAGTCATCCGCTGACCGAGGACCG
CCTGGCCCGGATGCGCGCGGAAGACCGCCCGCCGAGCGGCCCGCCGCTGCTCAGCGCGGAGGAATGGAAGGCGCTGAAGG
CGATTTGCAAGTAG

Upstream 100 bases:

>100_bases
GCGACGGTGCTCGAATCGGTCCATGTGCATGGCATCGAGGCCATGGCCAACGTCGCCGCCAAGGGCGATCTGGCCAGTGC
GCTCGGTGAAGGATTTGCCG

Downstream 100 bases:

>100_bases
CTGATTTGCGCAGCTCCTTCGCTAATCACTGGTGTCGTCCCGGCGAACGCCGGGACCCATAATCACCGCGTCGAGTTGTG
AGAAGCTGGTGTTGCTCAGC

Product: putative metalloendopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MDSTSPDSDSEPGAAEATRAVQVAPPGGPATYFDGLSNRRQNVTIGFANQLVMRSPDNFVSWPYADIRRVDGPNGTLRVS
CLSASPLARLEIRDAALVAELIARCSQIDQNRLTRGGIARIIAWSLAAVASIVTLVLVVIPFAADRLTPLVPPAMERHLG
EAAVTQIKTIYHAQLCSETAGQAAFAKLVTGLRRPAGLDDSIGSQVLDTPVPNAFALPGGQFFLFRGLLDTANDPDEVAG
VLAHELGHLKHRDNLRQLIHNGGSSFLIGLLFGDVTGAGAAIFASRAMINASYSREAEEAADSFAIEVMHKLGRPTRPMG
ELLFRITGKEAGKQPALWASHPLTEDRLARMRAEDRPPSGPPLLSAEEWKALKAICK

Sequences:

>Translated_377_residues
MDSTSPDSDSEPGAAEATRAVQVAPPGGPATYFDGLSNRRQNVTIGFANQLVMRSPDNFVSWPYADIRRVDGPNGTLRVS
CLSASPLARLEIRDAALVAELIARCSQIDQNRLTRGGIARIIAWSLAAVASIVTLVLVVIPFAADRLTPLVPPAMERHLG
EAAVTQIKTIYHAQLCSETAGQAAFAKLVTGLRRPAGLDDSIGSQVLDTPVPNAFALPGGQFFLFRGLLDTANDPDEVAG
VLAHELGHLKHRDNLRQLIHNGGSSFLIGLLFGDVTGAGAAIFASRAMINASYSREAEEAADSFAIEVMHKLGRPTRPMG
ELLFRITGKEAGKQPALWASHPLTEDRLARMRAEDRPPSGPPLLSAEEWKALKAICK
>Mature_377_residues
MDSTSPDSDSEPGAAEATRAVQVAPPGGPATYFDGLSNRRQNVTIGFANQLVMRSPDNFVSWPYADIRRVDGPNGTLRVS
CLSASPLARLEIRDAALVAELIARCSQIDQNRLTRGGIARIIAWSLAAVASIVTLVLVVIPFAADRLTPLVPPAMERHLG
EAAVTQIKTIYHAQLCSETAGQAAFAKLVTGLRRPAGLDDSIGSQVLDTPVPNAFALPGGQFFLFRGLLDTANDPDEVAG
VLAHELGHLKHRDNLRQLIHNGGSSFLIGLLFGDVTGAGAAIFASRAMINASYSREAEEAADSFAIEVMHKLGRPTRPMG
ELLFRITGKEAGKQPALWASHPLTEDRLARMRAEDRPPSGPPLLSAEEWKALKAICK

Specific function: Unknown

COG id: COG0501

COG function: function code O; Zn-dependent protease with chaperone function

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M48B family [H]

Homologues:

Organism=Escherichia coli, GI87081800, Length=207, Percent_Identity=28.5024154589372, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001915 [H]

Pfam domain/function: PF01435 Peptidase_M48 [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 40335; Mature: 40335

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSTSPDSDSEPGAAEATRAVQVAPPGGPATYFDGLSNRRQNVTIGFANQLVMRSPDNFV
CCCCCCCCCCCCCHHHHHCEEEECCCCCCHHHHHHHCCCCCEEEEEEHHHHHHCCCCCCC
SWPYADIRRVDGPNGTLRVSCLSASPLARLEIRDAALVAELIARCSQIDQNRLTRGGIAR
CCCHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIAWSLAAVASIVTLVLVVIPFAADRLTPLVPPAMERHLGEAAVTQIKTIYHAQLCSETA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQAAFAKLVTGLRRPAGLDDSIGSQVLDTPVPNAFALPGGQFFLFRGLLDTANDPDEVAG
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHCCCCHHHHHH
VLAHELGHLKHRDNLRQLIHNGGSSFLIGLLFGDVTGAGAAIFASRAMINASYSREAEEA
HHHHHHHHHHHHHHHHHHHHCCCCHHEHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHH
ADSFAIEVMHKLGRPTRPMGELLFRITGKEAGKQPALWASHPLTEDRLARMRAEDRPPSG
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
PPLLSAEEWKALKAICK
CCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MDSTSPDSDSEPGAAEATRAVQVAPPGGPATYFDGLSNRRQNVTIGFANQLVMRSPDNFV
CCCCCCCCCCCCCHHHHHCEEEECCCCCCHHHHHHHCCCCCEEEEEEHHHHHHCCCCCCC
SWPYADIRRVDGPNGTLRVSCLSASPLARLEIRDAALVAELIARCSQIDQNRLTRGGIAR
CCCHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIAWSLAAVASIVTLVLVVIPFAADRLTPLVPPAMERHLGEAAVTQIKTIYHAQLCSETA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQAAFAKLVTGLRRPAGLDDSIGSQVLDTPVPNAFALPGGQFFLFRGLLDTANDPDEVAG
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHCCCCHHHHHH
VLAHELGHLKHRDNLRQLIHNGGSSFLIGLLFGDVTGAGAAIFASRAMINASYSREAEEA
HHHHHHHHHHHHHHHHHHHHCCCCHHEHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHH
ADSFAIEVMHKLGRPTRPMGELLFRITGKEAGKQPALWASHPLTEDRLARMRAEDRPPSG
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
PPLLSAEEWKALKAICK
CCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7836281 [H]