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The map label for this gene is dosC [H]

Identifier: 146338453

GI number: 146338453

Start: 1454143

End: 1455390

Strand: Direct

Name: dosC [H]

Synonym: BRADO1369

Alternate gene names: 146338453

Gene position: 1454143-1455390 (Clockwise)

Preceding gene: 146338451

Following gene: 146338454

Centisome position: 19.5

GC content: 66.51

Gene sequence:

>1248_bases
TTGCGTCGTCCTGCGGTCGGGATGCTGAACGTTCCGACGCTGTGGACCGCCTTCGTGGTCAACTTCCTCGCGCTGGGCCT
GATCTGGGCCTACGTGATGCGCAGCTATCCGAATCTGGAATCGGCGCGCTACTGGACGGCGTCCGCCTTCATGTGCGCGC
CCGCCGCGCTGTTCGCGCTCGGCCGGTTCTATTTCGGATCCTACGTGCCGCTGCTCGCCGGCGCGTCGCTGATGACGCTG
GCCATCTGCCTCGCCGCAATGGGCGTCGAGCGCTTCTATGGCCAGAAGGCCTCCTGGCGGCTGAGCTTCGCGGTGGTCGC
CGTGACCTGCGCGTCGATCATCTTCTTTCTCGACGTCTACGACCACGGCCCGCTCCGGATCCTGTGCTATTCGATTGGTC
AGGCCATCCCGATGGCCATGATCGCGCGGCTGGTGCTCGATGCAAGAAACGGCCCGATCAACCCTGGCGCCCGGTTGGCC
GGGCTGTTGTCGCTTCTCATCATCGCGGTCTTCGCCGGCCGCTCGGTTGCGGGCTTTGTCGGCGCCGATTTCAACTTCAG
CCATTCGAGCAACGGCCAGGCGGCCGCGGTGCTGGTGCTGATTTTCCTGTCGATGTCGCTGAACTTCGGCTTTCTGCTGA
TGGCCGTGAACCGGCTGCGCAACGAGGCCGCCGATCTGGCGCTGCTCGACGACCTCACCGGCGTCTCCAACCGCCGCCAC
CTGATGCAGCGGCTCACCGAGGAGTGCGCCCGCGCCGAGCGCTCGGGGCGGGCGTTTGCGTTGCTGGTGATCGATCTCGA
CGGCTTCAAGCTCATCAACGACAATTATGGTCACGCGACCGGAGATGCCTGCCTGCAGCACTTCACGCTGATGGCCCAGA
CCAGGCTGCGCCCGGGCGACATGCTGGCGCGCACCGGCGGCGACGAGTTCTGTATTCTGCTGCCGTCCTCCTCGGTGCGC
GAGGGGGCGATGATCGCGCGGCGGATCCTGCAGGTCTGCCGCGACGATGCCGAGGCTTGCGCCGGGGCCGACGTGCCGAT
CGAGGTCTCGATCGGCGTCGCCGAATGGACCCGCGATGTCGGCGCGTTTCCGGACCGGGTGATGGCGCGGGCGGATCAGG
CGCTTTATGCGGCCAAGAAGGACGGCAAGAGCCGCTTCGCCGTCTACCAGGACGCGCCCGCTTTGGCGCCCGAAGAGATC
CATCCCATCGCCATGCACCAGCCGAAGCAGGATGCCGCGGCTGAGTAG

Upstream 100 bases:

>100_bases
GGTGGAGGAATTGAGGAGCCGTTAAGTGCGCCGCGCTCATGTTGCCGCCGATGACAGGGAAGGCCATGAGTACGGCGTTG
GTGAATGTTAAGGACAGGAC

Downstream 100 bases:

>100_bases
CGGTGCTATCGTTCCGGCCATGCGGCCGATCCGAACCTTCCTCAAGGCAGCCGTCGCGCTGCTTCTTCTCATGGACGCGG
CGTCCGCCGACGATCTCGCC

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MRRPAVGMLNVPTLWTAFVVNFLALGLIWAYVMRSYPNLESARYWTASAFMCAPAALFALGRFYFGSYVPLLAGASLMTL
AICLAAMGVERFYGQKASWRLSFAVVAVTCASIIFFLDVYDHGPLRILCYSIGQAIPMAMIARLVLDARNGPINPGARLA
GLLSLLIIAVFAGRSVAGFVGADFNFSHSSNGQAAAVLVLIFLSMSLNFGFLLMAVNRLRNEAADLALLDDLTGVSNRRH
LMQRLTEECARAERSGRAFALLVIDLDGFKLINDNYGHATGDACLQHFTLMAQTRLRPGDMLARTGGDEFCILLPSSSVR
EGAMIARRILQVCRDDAEACAGADVPIEVSIGVAEWTRDVGAFPDRVMARADQALYAAKKDGKSRFAVYQDAPALAPEEI
HPIAMHQPKQDAAAE

Sequences:

>Translated_415_residues
MRRPAVGMLNVPTLWTAFVVNFLALGLIWAYVMRSYPNLESARYWTASAFMCAPAALFALGRFYFGSYVPLLAGASLMTL
AICLAAMGVERFYGQKASWRLSFAVVAVTCASIIFFLDVYDHGPLRILCYSIGQAIPMAMIARLVLDARNGPINPGARLA
GLLSLLIIAVFAGRSVAGFVGADFNFSHSSNGQAAAVLVLIFLSMSLNFGFLLMAVNRLRNEAADLALLDDLTGVSNRRH
LMQRLTEECARAERSGRAFALLVIDLDGFKLINDNYGHATGDACLQHFTLMAQTRLRPGDMLARTGGDEFCILLPSSSVR
EGAMIARRILQVCRDDAEACAGADVPIEVSIGVAEWTRDVGAFPDRVMARADQALYAAKKDGKSRFAVYQDAPALAPEEI
HPIAMHQPKQDAAAE
>Mature_415_residues
MRRPAVGMLNVPTLWTAFVVNFLALGLIWAYVMRSYPNLESARYWTASAFMCAPAALFALGRFYFGSYVPLLAGASLMTL
AICLAAMGVERFYGQKASWRLSFAVVAVTCASIIFFLDVYDHGPLRILCYSIGQAIPMAMIARLVLDARNGPINPGARLA
GLLSLLIIAVFAGRSVAGFVGADFNFSHSSNGQAAAVLVLIFLSMSLNFGFLLMAVNRLRNEAADLALLDDLTGVSNRRH
LMQRLTEECARAERSGRAFALLVIDLDGFKLINDNYGHATGDACLQHFTLMAQTRLRPGDMLARTGGDEFCILLPSSSVR
EGAMIARRILQVCRDDAEACAGADVPIEVSIGVAEWTRDVGAFPDRVMARADQALYAAKKDGKSRFAVYQDAPALAPEEI
HPIAMHQPKQDAAAE

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=37.5757575757576, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787262, Length=171, Percent_Identity=30.9941520467836, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=36.4705882352941, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786584, Length=159, Percent_Identity=35.8490566037736, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=32.9192546583851, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787802, Length=178, Percent_Identity=31.4606741573034, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=187, Percent_Identity=29.4117647058824, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=34.5029239766082, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=32.2981366459627, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=205, Percent_Identity=31.219512195122, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081974, Length=171, Percent_Identity=30.9941520467836, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081977, Length=187, Percent_Identity=31.5508021390374, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45040; Mature: 45040

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPAVGMLNVPTLWTAFVVNFLALGLIWAYVMRSYPNLESARYWTASAFMCAPAALFAL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHH
GRFYFGSYVPLLAGASLMTLAICLAAMGVERFYGQKASWRLSFAVVAVTCASIIFFLDVY
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
DHGPLRILCYSIGQAIPMAMIARLVLDARNGPINPGARLAGLLSLLIIAVFAGRSVAGFV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
GADFNFSHSSNGQAAAVLVLIFLSMSLNFGFLLMAVNRLRNEAADLALLDDLTGVSNRRH
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LMQRLTEECARAERSGRAFALLVIDLDGFKLINDNYGHATGDACLQHFTLMAQTRLRPGD
HHHHHHHHHHHHHHCCCEEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCH
MLARTGGDEFCILLPSSSVREGAMIARRILQVCRDDAEACAGADVPIEVSIGVAEWTRDV
HHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHH
GAFPDRVMARADQALYAAKKDGKSRFAVYQDAPALAPEEIHPIAMHQPKQDAAAE
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRPAVGMLNVPTLWTAFVVNFLALGLIWAYVMRSYPNLESARYWTASAFMCAPAALFAL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHH
GRFYFGSYVPLLAGASLMTLAICLAAMGVERFYGQKASWRLSFAVVAVTCASIIFFLDVY
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
DHGPLRILCYSIGQAIPMAMIARLVLDARNGPINPGARLAGLLSLLIIAVFAGRSVAGFV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
GADFNFSHSSNGQAAAVLVLIFLSMSLNFGFLLMAVNRLRNEAADLALLDDLTGVSNRRH
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LMQRLTEECARAERSGRAFALLVIDLDGFKLINDNYGHATGDACLQHFTLMAQTRLRPGD
HHHHHHHHHHHHHHCCCEEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCH
MLARTGGDEFCILLPSSSVREGAMIARRILQVCRDDAEACAGADVPIEVSIGVAEWTRDV
HHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHH
GAFPDRVMARADQALYAAKKDGKSRFAVYQDAPALAPEEIHPIAMHQPKQDAAAE
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]