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The map label for this gene is lhgO [H]

Identifier: 146338307

GI number: 146338307

Start: 1284863

End: 1285966

Strand: Reverse

Name: lhgO [H]

Synonym: BRADO1212

Alternate gene names: 146338307

Gene position: 1285966-1284863 (Counterclockwise)

Preceding gene: 146338313

Following gene: 146338305

Centisome position: 17.25

GC content: 65.76

Gene sequence:

>1104_bases
ATGGATAGAGTGGATTGCGTCGTCATCGGCGCCGGAGTCGTGGGGCTCGCGATCGCCCGCCAATTGGCTGGAGCGGGCCG
TGAAGTCATCGTCCTCGAGGCCGCCGAGGACATCGGCACGGGGACATCGTCTCGCAATAGCGAAGTGATCCATGCCGGCA
TCTACTACAAGGCCGGGAGCCTGATGGCCCGCTTCTGCGTGACCGGCAAGCGGATGCTCTACCGCTACTGCGACGAGCAT
GGCGTTCCCTATCGCAATTGCGGCAAGCTCATCGTTGCGACCAGCCCGGCGGAGACCGAGAAGCTGCAGGGCATCCGAGC
GCATGCCGAGGCGAACGGCGTCGAAGGCATGCGGCTCCTGACCGGTTCCGAGGCGCGGACGTTGGAGCCCGCCCTCGCCT
GCGAAGCCGCGTTGCTGTCGCCCTCCACCGGGATCCTCGACAGCCACGCCTTCATGCTGTCGCTGCGCGGCGAGATCGAG
GATGCGGGCGGCGCCTTGGCTTTCCACACCCCGCTGCTGCGCGCCAAGGCGACCGGGGACGGAATCGAGCTCGAAGCCGG
CGGCGAGCAGCCGATGATGTTGTCATGTCGGCTGCTGGTCAACGCCGCGGGCCTGAGCGCGCCGGCGGTGGCACGAGGGA
TCGACGGGATGCCGCCGCAGCTGGTGCCAACGGCCTATCTCGCCAAGGGCAATTATTTCAGCTGCAGCGCCAAGGCTCCC
TTCAGCCGTCTGATCTATCCGGTGCCGGAACCCGGCGGACTCGGCGTCCACCTCACGCTCGACATGGCGGGCCAGGCCCG
CTTCGGCCCTGACGTCGAGTGGATCGATACGATCGACTACACCGTCGATCCCGCTCGTGCGGAACGCTTCTACCCAGCGA
TCCGAAAATATTGGCCGACGCTACCCGACGGCGCGCTGATGCCGAGCTATTCGGGGATCAGGCCGAAGATCGTCCCTCCC
GCGGTGGCGTCGCAGGACTTCGTCGTTCAGGGACCGCGCGATCACGGTGTAGACGGACTCATCAATCTGTTCGGAATCGA
ATCGCCTGGACTGACGTCATCGCTTGCGATCGCCGCCCATGTCGGCGAACTCAGCGGTATCTGA

Upstream 100 bases:

>100_bases
TGATGAATACTTTGCTAAGGAGTGACGGACTTCGGACCGAGTTTCGCCCACCGGAGATTGCAACGAAACATGACTTCTCG
GAACCGTTTCGGAAACGCGA

Downstream 100 bases:

>100_bases
AGAACTCACCATCAGTGCGGCGTTATCTGTCGGACGACGCTCACTTCGCGCCGCGGCATGCCGGGCTCAATTCGTCATAT
CTTTAAGGAAGTTGCGCCTG

Product: NAD binding site:FAD dependent oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MDRVDCVVIGAGVVGLAIARQLAGAGREVIVLEAAEDIGTGTSSRNSEVIHAGIYYKAGSLMARFCVTGKRMLYRYCDEH
GVPYRNCGKLIVATSPAETEKLQGIRAHAEANGVEGMRLLTGSEARTLEPALACEAALLSPSTGILDSHAFMLSLRGEIE
DAGGALAFHTPLLRAKATGDGIELEAGGEQPMMLSCRLLVNAAGLSAPAVARGIDGMPPQLVPTAYLAKGNYFSCSAKAP
FSRLIYPVPEPGGLGVHLTLDMAGQARFGPDVEWIDTIDYTVDPARAERFYPAIRKYWPTLPDGALMPSYSGIRPKIVPP
AVASQDFVVQGPRDHGVDGLINLFGIESPGLTSSLAIAAHVGELSGI

Sequences:

>Translated_367_residues
MDRVDCVVIGAGVVGLAIARQLAGAGREVIVLEAAEDIGTGTSSRNSEVIHAGIYYKAGSLMARFCVTGKRMLYRYCDEH
GVPYRNCGKLIVATSPAETEKLQGIRAHAEANGVEGMRLLTGSEARTLEPALACEAALLSPSTGILDSHAFMLSLRGEIE
DAGGALAFHTPLLRAKATGDGIELEAGGEQPMMLSCRLLVNAAGLSAPAVARGIDGMPPQLVPTAYLAKGNYFSCSAKAP
FSRLIYPVPEPGGLGVHLTLDMAGQARFGPDVEWIDTIDYTVDPARAERFYPAIRKYWPTLPDGALMPSYSGIRPKIVPP
AVASQDFVVQGPRDHGVDGLINLFGIESPGLTSSLAIAAHVGELSGI
>Mature_367_residues
MDRVDCVVIGAGVVGLAIARQLAGAGREVIVLEAAEDIGTGTSSRNSEVIHAGIYYKAGSLMARFCVTGKRMLYRYCDEH
GVPYRNCGKLIVATSPAETEKLQGIRAHAEANGVEGMRLLTGSEARTLEPALACEAALLSPSTGILDSHAFMLSLRGEIE
DAGGALAFHTPLLRAKATGDGIELEAGGEQPMMLSCRLLVNAAGLSAPAVARGIDGMPPQLVPTAYLAKGNYFSCSAKAP
FSRLIYPVPEPGGLGVHLTLDMAGQARFGPDVEWIDTIDYTVDPARAERFYPAIRKYWPTLPDGALMPSYSGIRPKIVPP
AVASQDFVVQGPRDHGVDGLINLFGIESPGLTSSLAIAAHVGELSGI

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=264, Percent_Identity=32.5757575757576, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI87082148, Length=376, Percent_Identity=27.6595744680851, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17565764, Length=281, Percent_Identity=33.8078291814947, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24585081, Length=382, Percent_Identity=28.7958115183246, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 38626; Mature: 38626

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRVDCVVIGAGVVGLAIARQLAGAGREVIVLEAAEDIGTGTSSRNSEVIHAGIYYKAGS
CCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEEECHHCCCCCCCCCCCEEEEEEEEEHHH
LMARFCVTGKRMLYRYCDEHGVPYRNCGKLIVATSPAETEKLQGIRAHAEANGVEGMRLL
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCCCEEEE
TGSEARTLEPALACEAALLSPSTGILDSHAFMLSLRGEIEDAGGALAFHTPLLRAKATGD
ECCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCEEEECCCC
GIELEAGGEQPMMLSCRLLVNAAGLSAPAVARGIDGMPPQLVPTAYLAKGNYFSCSAKAP
CEEEECCCCCCEEEEEEEEEECCCCCCCHHHHCCCCCCCCCCCEEEEECCCEEEECCCCC
FSRLIYPVPEPGGLGVHLTLDMAGQARFGPDVEWIDTIDYTVDPARAERFYPAIRKYWPT
HHHCEEECCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCEECHHHHHHHHHHHHHHCCC
LPDGALMPSYSGIRPKIVPPAVASQDFVVQGPRDHGVDGLINLFGIESPGLTSSLAIAAH
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEHEEECCCCCCCHHHHHEEH
VGELSGI
HHHHCCC
>Mature Secondary Structure
MDRVDCVVIGAGVVGLAIARQLAGAGREVIVLEAAEDIGTGTSSRNSEVIHAGIYYKAGS
CCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEEECHHCCCCCCCCCCCEEEEEEEEEHHH
LMARFCVTGKRMLYRYCDEHGVPYRNCGKLIVATSPAETEKLQGIRAHAEANGVEGMRLL
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCCCEEEE
TGSEARTLEPALACEAALLSPSTGILDSHAFMLSLRGEIEDAGGALAFHTPLLRAKATGD
ECCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCEEEECCCC
GIELEAGGEQPMMLSCRLLVNAAGLSAPAVARGIDGMPPQLVPTAYLAKGNYFSCSAKAP
CEEEECCCCCCEEEEEEEEEECCCCCCCHHHHCCCCCCCCCCCEEEEECCCEEEECCCCC
FSRLIYPVPEPGGLGVHLTLDMAGQARFGPDVEWIDTIDYTVDPARAERFYPAIRKYWPT
HHHCEEECCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCEECHHHHHHHHHHHHHHCCC
LPDGALMPSYSGIRPKIVPPAVASQDFVVQGPRDHGVDGLINLFGIESPGLTSSLAIAAH
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEHEEECCCCCCCHHHHHEEH
VGELSGI
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]