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The map label for this gene is pntA2 [H]

Identifier: 146338292

GI number: 146338292

Start: 1272319

End: 1273446

Strand: Direct

Name: pntA2 [H]

Synonym: BRADO1196

Alternate gene names: 146338292

Gene position: 1272319-1273446 (Clockwise)

Preceding gene: 146338291

Following gene: 146338293

Centisome position: 17.06

GC content: 65.43

Gene sequence:

>1128_bases
ATGAAGATTGCCGTCGCCAAGGAAATCGACCCGTTGGAGCCGCGGGTCGCCGCCTCTCCCGACACGGTGAAGAAGTTCAA
GGCGCTGGGTGCCGAGATCGCGGTCGAGCCCGGCGCCGGCATCAAGTCGGGCCTGCCGGATTCGGAATTCACGGCGGCGG
GTGCAACCGTAAGTGCTGATGCGCTCAAGGACGCCGATATCATCATCAAGGTGAAGCGGCCGGAGGCCTCCGAACTCGCC
AACTACCGCCGCGGCGCGCTCGTGTTCGCGATCATGGATCCCTACGGCAACGACGCCGCGCTGAAAGCGATGGCCGACGC
CGGCATCGCGTCCTTCGCGATGGAACTGATGCCACGCATCACCCGCGCGCAGGTCATGGACGTATTGTCGAGCCAGGCGA
ACCTCGCCGGTTATCGCGCTGTCATCGAAGGCGCCGAGGCATTCGGCCGCGCCTTCCCGATGATGATGACCGCGGCCGGC
ACCGTGCCGGCAACCAAGGTCTTCGTGATGGGCGTCGGCGTCGCCGGCCTGCAGGCGATCGCGACCGCGCGTCGCCTTGG
CGCCATCGTCTCCGCCACTGACGTGCGCCCCGCCACCAAGGAGCAGGTCGAGAGTCTCGGCGCGAAGTTCCTGGCCGTCG
AGGACGAGGAGTTCAAGAACGCGCAGACCGCCGGCGGCTACGCCAAGGAGATGTCCAAGGAGTACCAGGCCAAGCAGGCT
GCTCTGACGGCGGAACACATCAAGAAGCAGGACGTCATTATCACGACCGCGCTGATCCCCGGCCGGCCGGCGCCGCGCCT
CGTCACCCTGGAGATGGTGAAGTCGATGCGTCCCGGCTCGGTGCTGGTCGACCTCGCGGTCGAACGCGGCGGCAATGTCG
AAGGCGTCAAGCTGGGCGAGACCGTCGAGATCGACGGCGTCAAGATCATCGGTTTCCCGAACCTCGCCGGCCGCGTCGCG
GCGTCGGCTTCTGGCCTCTACGCCCGCAACCTGTTCTCCTTCATCGAGACGATGATCGATAAGAAGGAGAAGACGCTGGC
CGTGAACTGGGACGACGAGCTGGTCAAGGCCACGGCTTTGACAAAGGACGGCGCCATCGTCCACCCGAATTTCCAGCCTA
AGGCTTAG

Upstream 100 bases:

>100_bases
GCTGACTGCCGCAGCCGATGGCTGACCCTGGCGTCACGCTGGAGCCCATGGACGTCCCGCCCGTTTTTAAGCTCGCGCGC
GCATCCGCGCCGGAGGCCTC

Downstream 100 bases:

>100_bases
TTGAGGATCTAGGAGCAAAGCCATGGAACATGTTGTCCAAGCCGTCGATCCCTTCGTTTTCCGGCTGTCGATCTTCGTCC
TCGCCGTCTTCGTCGGCTAC

Product: nicotinamide nucleotide transhydrogenase subunit alpha1

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Proton-translocating transhydrogenase component 1; Pyridine nucleotide transhydrogenase subunit alpha 1; dI [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKIAVAKEIDPLEPRVAASPDTVKKFKALGAEIAVEPGAGIKSGLPDSEFTAAGATVSADALKDADIIIKVKRPEASELA
NYRRGALVFAIMDPYGNDAALKAMADAGIASFAMELMPRITRAQVMDVLSSQANLAGYRAVIEGAEAFGRAFPMMMTAAG
TVPATKVFVMGVGVAGLQAIATARRLGAIVSATDVRPATKEQVESLGAKFLAVEDEEFKNAQTAGGYAKEMSKEYQAKQA
ALTAEHIKKQDVIITTALIPGRPAPRLVTLEMVKSMRPGSVLVDLAVERGGNVEGVKLGETVEIDGVKIIGFPNLAGRVA
ASASGLYARNLFSFIETMIDKKEKTLAVNWDDELVKATALTKDGAIVHPNFQPKA

Sequences:

>Translated_375_residues
MKIAVAKEIDPLEPRVAASPDTVKKFKALGAEIAVEPGAGIKSGLPDSEFTAAGATVSADALKDADIIIKVKRPEASELA
NYRRGALVFAIMDPYGNDAALKAMADAGIASFAMELMPRITRAQVMDVLSSQANLAGYRAVIEGAEAFGRAFPMMMTAAG
TVPATKVFVMGVGVAGLQAIATARRLGAIVSATDVRPATKEQVESLGAKFLAVEDEEFKNAQTAGGYAKEMSKEYQAKQA
ALTAEHIKKQDVIITTALIPGRPAPRLVTLEMVKSMRPGSVLVDLAVERGGNVEGVKLGETVEIDGVKIIGFPNLAGRVA
ASASGLYARNLFSFIETMIDKKEKTLAVNWDDELVKATALTKDGAIVHPNFQPKA
>Mature_375_residues
MKIAVAKEIDPLEPRVAASPDTVKKFKALGAEIAVEPGAGIKSGLPDSEFTAAGATVSADALKDADIIIKVKRPEASELA
NYRRGALVFAIMDPYGNDAALKAMADAGIASFAMELMPRITRAQVMDVLSSQANLAGYRAVIEGAEAFGRAFPMMMTAAG
TVPATKVFVMGVGVAGLQAIATARRLGAIVSATDVRPATKEQVESLGAKFLAVEDEEFKNAQTAGGYAKEMSKEYQAKQA
ALTAEHIKKQDVIITTALIPGRPAPRLVTLEMVKSMRPGSVLVDLAVERGGNVEGVKLGETVEIDGVKIIGFPNLAGRVA
ASASGLYARNLFSFIETMIDKKEKTLAVNWDDELVKATALTKDGAIVHPNFQPKA

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=388, Percent_Identity=38.4020618556701, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI122939153, Length=388, Percent_Identity=38.4020618556701, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1787887, Length=372, Percent_Identity=43.5483870967742, Blast_Score=288, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17550456, Length=367, Percent_Identity=45.5040871934605, Blast_Score=304, Evalue=5e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 39529; Mature: 39529

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVAKEIDPLEPRVAASPDTVKKFKALGAEIAVEPGAGIKSGLPDSEFTAAGATVSAD
CCEECCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEHH
ALKDADIIIKVKRPEASELANYRRGALVFAIMDPYGNDAALKAMADAGIASFAMELMPRI
HCCCCEEEEEECCCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
TRAQVMDVLSSQANLAGYRAVIEGAEAFGRAFPMMMTAAGTVPATKVFVMGVGVAGLQAI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEECCHHHHHHH
ATARRLGAIVSATDVRPATKEQVESLGAKFLAVEDEEFKNAQTAGGYAKEMSKEYQAKQA
HHHHHHHHHHEECCCCCCCHHHHHHCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHH
ALTAEHIKKQDVIITTALIPGRPAPRLVTLEMVKSMRPGSVLVDLAVERGGNVEGVKLGE
HHHHHHHCCCCEEEEEEECCCCCCCCEEEHHHHHCCCCCCEEEEEEECCCCCCCEEEECC
TVEIDGVKIIGFPNLAGRVAASASGLYARNLFSFIETMIDKKEKTLAVNWDDELVKATAL
EEEECCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHEE
TKDGAIVHPNFQPKA
CCCCCEECCCCCCCC
>Mature Secondary Structure
MKIAVAKEIDPLEPRVAASPDTVKKFKALGAEIAVEPGAGIKSGLPDSEFTAAGATVSAD
CCEECCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEHH
ALKDADIIIKVKRPEASELANYRRGALVFAIMDPYGNDAALKAMADAGIASFAMELMPRI
HCCCCEEEEEECCCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
TRAQVMDVLSSQANLAGYRAVIEGAEAFGRAFPMMMTAAGTVPATKVFVMGVGVAGLQAI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEECCHHHHHHH
ATARRLGAIVSATDVRPATKEQVESLGAKFLAVEDEEFKNAQTAGGYAKEMSKEYQAKQA
HHHHHHHHHHEECCCCCCCHHHHHHCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHH
ALTAEHIKKQDVIITTALIPGRPAPRLVTLEMVKSMRPGSVLVDLAVERGGNVEGVKLGE
HHHHHHHCCCCEEEEEEECCCCCCCCEEEHHHHHCCCCCCEEEEEEECCCCCCCEEEECC
TVEIDGVKIIGFPNLAGRVAASASGLYARNLFSFIETMIDKKEKTLAVNWDDELVKATAL
EEEECCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHEE
TKDGAIVHPNFQPKA
CCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]