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The map label for this gene is ruvB [H]

Identifier: 146338243

GI number: 146338243

Start: 1219092

End: 1220138

Strand: Direct

Name: ruvB [H]

Synonym: BRADO1147

Alternate gene names: 146338243

Gene position: 1219092-1220138 (Clockwise)

Preceding gene: 146338242

Following gene: 146338244

Centisome position: 16.35

GC content: 68.39

Gene sequence:

>1047_bases
ATGAAGCCGCCGGCGCGCATGGTCAGCCCGGAGCGGCGCTCCGACGATGTCGGTGACACCGCGCTGCGGCCGCAGCAGCT
GTCGGAGTTCGTCGGCCAGCAGCAGGCGCGCGCCAATCTGTCGATCTTCATCGAGGCGGCGCGCAAGCGCGGCGAGGCGC
TCGATCACGTGCTGTTCGTCGGTCCTCCGGGTCTTGGCAAGACCACGCTGGCGCAGATCGTCGCGCGCGAGCTCGGCGTC
GGCTTCCGTGCCACGTCCGGCCCGGTGATAGCGAAGGCGGGCGATCTCGCGGCGCTGCTGACCAATCTCGAGGAGCGCGA
CGTGCTGTTCATCGACGAGATCCATCGGCTCAGCCCGGCGGTGGAGGAGGTGCTCTATCCGGCAATGGAGGACTTCCAGC
TCGATCTCATCATCGGCGAGGGACCAGCGGCGCGCTCGGTCAAGATCGATCTGTCGAAGTTCACGCTGGTCGGCGCGACG
ACGCGGGCGGGCCTGCTGACAAATCCGCTACGCGACCGTTTCGGCATTCCAATCCGCTTGAACTTCTACACCGTCGAGGA
GCTCGAAGGCATCGTCACTCGCGGCGCCCGTGTGCTCGGGATCGGCATGACCCCCGATGGCGCCAACGAGATCGCGCGCC
GCGCCCGCGGCACGCCGCGCATCGCCGGCCGCCTGCTGCGCCGGGTGCGCGACTTCGCCTCCGCGGCCGACGCCAGCGCG
ATCGATCGCGCCATCGCCGATCACGCGCTGAGCGCGCTGGAGGTCGACGCGGCCGGGCTCGATGCGATGGATCGGCGCTA
TCTCTCGACCATCGCGCTGAACTACGGCGGCGGCCCGGTCGGCGTCGAGACGATGGCGGCGGCGCTGTCGGAGCCGCGCG
ATGCGATCGAGGACATCATCGAGCCTTATCTCATTCAATGCGGCTATCTGCAGCGCACCCCGCGCGGCCGGCTGTTGACC
TCGCATGCGTTCAAGCATCTCGGCATGGCCGAGCCCGCGAACCGCGATCCCAGCCAGATCGGCCTGTTTGGCAACGACGA
TGATTAA

Upstream 100 bases:

>100_bases
ATGATGCGAAAGCGCGGGTCATCGTGCCGTCGAACGGCAAGGGCCCCGACGTCGTGACGATCGGCGAACTGCTGCCGAAC
AAATACCGGCGCGGCAACAA

Downstream 100 bases:

>100_bases
CGAAATCTGCTGACAATCTGCACAGACGCACCCGAAATCGGTTGCAATGAACGAGGAAAAGGATTGCGTGCGAGGAGTTC
GTCCATGCTGTCACGCCGTC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKPPARMVSPERRSDDVGDTALRPQQLSEFVGQQQARANLSIFIEAARKRGEALDHVLFVGPPGLGKTTLAQIVARELGV
GFRATSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPAVEEVLYPAMEDFQLDLIIGEGPAARSVKIDLSKFTLVGAT
TRAGLLTNPLRDRFGIPIRLNFYTVEELEGIVTRGARVLGIGMTPDGANEIARRARGTPRIAGRLLRRVRDFASAADASA
IDRAIADHALSALEVDAAGLDAMDRRYLSTIALNYGGGPVGVETMAAALSEPRDAIEDIIEPYLIQCGYLQRTPRGRLLT
SHAFKHLGMAEPANRDPSQIGLFGNDDD

Sequences:

>Translated_348_residues
MKPPARMVSPERRSDDVGDTALRPQQLSEFVGQQQARANLSIFIEAARKRGEALDHVLFVGPPGLGKTTLAQIVARELGV
GFRATSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPAVEEVLYPAMEDFQLDLIIGEGPAARSVKIDLSKFTLVGAT
TRAGLLTNPLRDRFGIPIRLNFYTVEELEGIVTRGARVLGIGMTPDGANEIARRARGTPRIAGRLLRRVRDFASAADASA
IDRAIADHALSALEVDAAGLDAMDRRYLSTIALNYGGGPVGVETMAAALSEPRDAIEDIIEPYLIQCGYLQRTPRGRLLT
SHAFKHLGMAEPANRDPSQIGLFGNDDD
>Mature_348_residues
MKPPARMVSPERRSDDVGDTALRPQQLSEFVGQQQARANLSIFIEAARKRGEALDHVLFVGPPGLGKTTLAQIVARELGV
GFRATSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPAVEEVLYPAMEDFQLDLIIGEGPAARSVKIDLSKFTLVGAT
TRAGLLTNPLRDRFGIPIRLNFYTVEELEGIVTRGARVLGIGMTPDGANEIARRARGTPRIAGRLLRRVRDFASAADASA
IDRAIADHALSALEVDAAGLDAMDRRYLSTIALNYGGGPVGVETMAAALSEPRDAIEDIIEPYLIQCGYLQRTPRGRLLT
SHAFKHLGMAEPANRDPSQIGLFGNDDD

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=318, Percent_Identity=64.7798742138365, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1787119, Length=124, Percent_Identity=33.8709677419355, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37580; Mature: 37580

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPPARMVSPERRSDDVGDTALRPQQLSEFVGQQQARANLSIFIEAARKRGEALDHVLFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCEEEEE
GPPGLGKTTLAQIVARELGVGFRATSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPA
CCCCCCHHHHHHHHHHHHCCCEECCCCCEEECCCHHHHHHHCCCCCCEEHHHHHHHHHHH
VEEVLYPAMEDFQLDLIIGEGPAARSVKIDLSKFTLVGATTRAGLLTNPLRDRFGIPIRL
HHHHHHHHHHCCEEEEEECCCCCCCEEEEEHHHEEEEECCHHCCHHHCCHHHHCCCCEEE
NFYTVEELEGIVTRGARVLGIGMTPDGANEIARRARGTPRIAGRLLRRVRDFASAADASA
EEEEHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHH
IDRAIADHALSALEVDAAGLDAMDRRYLSTIALNYGGGPVGVETMAAALSEPRDAIEDII
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHCHHHHHHHHH
EPYLIQCGYLQRTPRGRLLTSHAFKHLGMAEPANRDPSQIGLFGNDDD
HHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure
MKPPARMVSPERRSDDVGDTALRPQQLSEFVGQQQARANLSIFIEAARKRGEALDHVLFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCEEEEE
GPPGLGKTTLAQIVARELGVGFRATSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPA
CCCCCCHHHHHHHHHHHHCCCEECCCCCEEECCCHHHHHHHCCCCCCEEHHHHHHHHHHH
VEEVLYPAMEDFQLDLIIGEGPAARSVKIDLSKFTLVGATTRAGLLTNPLRDRFGIPIRL
HHHHHHHHHHCCEEEEEECCCCCCCEEEEEHHHEEEEECCHHCCHHHCCHHHHCCCCEEE
NFYTVEELEGIVTRGARVLGIGMTPDGANEIARRARGTPRIAGRLLRRVRDFASAADASA
EEEEHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHH
IDRAIADHALSALEVDAAGLDAMDRRYLSTIALNYGGGPVGVETMAAALSEPRDAIEDII
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHCHHHHHHHHH
EPYLIQCGYLQRTPRGRLLTSHAFKHLGMAEPANRDPSQIGLFGNDDD
HHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]