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The map label for this gene is acrE [H]

Identifier: 146338215

GI number: 146338215

Start: 1186657

End: 1187790

Strand: Direct

Name: acrE [H]

Synonym: BRADO1114

Alternate gene names: 146338215

Gene position: 1186657-1187790 (Clockwise)

Preceding gene: 146338214

Following gene: 146338216

Centisome position: 15.91

GC content: 68.17

Gene sequence:

>1134_bases
TTGGCAGGCTGCAACGACACCACGCCCGCGCAGGCCTCGGCCAACGATCAGATCGCCGTGCCCGACGTCGGCATCATCAC
CGTCGAGCAGAAGTCGCGCGCGATCGTCCGCGAGCTGCCCGGCCGTATCGCGCCGACCCGCGTCTCTGATGTGCGGCCGC
GCGTCTCCGGCATTATCGTCGAGCGGCTGTTCCGCCAGGGCAGCGAGGTCAAGGCCGGCGATCCGCTCTATCGGATCGAT
CCCAAACCGTTCGAGGTGGAAATCCTGTCGAGCCGCGCGGCGCTGGCCAAGGCCGAGGCGGTGCACGAGCGCGCCGTGCA
GCAGGCGCGCCGCATCGCGTTGCTGTTCAAGGAGCGCGCCGCGCCCGAAGTCGAGAATGAGAAAGCGATCTCGTCAGAAC
GCGAGGCCACGGCCGATATCGAGGCCCGCAAGGCGGATCTCGCACGCGCGCAGCTCAATCTCGACTACGCCACCGTGCGC
GCGCCGATCGACGGCATCGTCGGCGCCGCGCAGGTCAGCGAAGGCGCCATCGCCGTCCAGAACGAGACCTCGCTGGTGAC
CATCCAGCAGCTTGATCCGATCTACGCCGACTTCACTCAGTCGGTCAGTGAGCTGAACCGGCTGCGCCGCGCCTTCGAGT
CCGGTGATCTCGACCAGATCGCACCCGATGCGATCAAGGTCCGGCTCGTGCTCGACGACGGCACGCCGTATGCGATCCCC
GGCAAGCTGCTGTTCTCCGACGCCAAGGTCGATGCCGCGACCGGGCAGGTGACCTTGCGCGGCGAGTTCGCCAATCCCAA
GCGCGAGCTGCTCCCTGGCATGTATGTTCGCGTCCGCATCGAGCAGGGCATCGACAGCGACTCGGTCGCGGTGCCGCAGC
AGGCGGTGCAGCGCAACGCCGCCGGCGGCTCCGAGGTCTATGTCATCCGCGAGGACAGCCGCGCCATCGCGCGTCCGATC
CGCACCGGACCGGTGCAGGATGGTCTCGTCCTGATCACCGACGGCCTGCGCGCCGGCGACAAGGTCGTCGTCGACGGCTT
CCAGAAATTTGCCGCCGGCGACAAGGTCGCGCCGCAGGCCTGGACCGAGGTCAGCACGGCGACGCAGCTCGAAACCAAGT
CGGCGTCGCGGTAA

Upstream 100 bases:

>100_bases
GTCGACCGCTTTCAAACATGATCGAGGGGCTCCATTGTCCATGTCTCGTTTTCGCGCGCCGCTCATCGGCAGCGCCTTGA
TGCTCGCAGCGGCTCTTCCT

Downstream 100 bases:

>100_bases
GTCCTGATGCCGAGCTTCTTCATCGACAGGCCGATCTTCGCCTGGGTCGTCGCGCTGTTCATCTGCCTGATCGGCGCCAT
CGCGATCCCGCTGCTGGCGG

Product: AcrB/AcrD/AcrF family mulitdrug efflux protein

Products: NA

Alternate protein names: Protein envC [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MAGCNDTTPAQASANDQIAVPDVGIITVEQKSRAIVRELPGRIAPTRVSDVRPRVSGIIVERLFRQGSEVKAGDPLYRID
PKPFEVEILSSRAALAKAEAVHERAVQQARRIALLFKERAAPEVENEKAISSEREATADIEARKADLARAQLNLDYATVR
APIDGIVGAAQVSEGAIAVQNETSLVTIQQLDPIYADFTQSVSELNRLRRAFESGDLDQIAPDAIKVRLVLDDGTPYAIP
GKLLFSDAKVDAATGQVTLRGEFANPKRELLPGMYVRVRIEQGIDSDSVAVPQQAVQRNAAGGSEVYVIREDSRAIARPI
RTGPVQDGLVLITDGLRAGDKVVVDGFQKFAAGDKVAPQAWTEVSTATQLETKSASR

Sequences:

>Translated_377_residues
MAGCNDTTPAQASANDQIAVPDVGIITVEQKSRAIVRELPGRIAPTRVSDVRPRVSGIIVERLFRQGSEVKAGDPLYRID
PKPFEVEILSSRAALAKAEAVHERAVQQARRIALLFKERAAPEVENEKAISSEREATADIEARKADLARAQLNLDYATVR
APIDGIVGAAQVSEGAIAVQNETSLVTIQQLDPIYADFTQSVSELNRLRRAFESGDLDQIAPDAIKVRLVLDDGTPYAIP
GKLLFSDAKVDAATGQVTLRGEFANPKRELLPGMYVRVRIEQGIDSDSVAVPQQAVQRNAAGGSEVYVIREDSRAIARPI
RTGPVQDGLVLITDGLRAGDKVVVDGFQKFAAGDKVAPQAWTEVSTATQLETKSASR
>Mature_376_residues
AGCNDTTPAQASANDQIAVPDVGIITVEQKSRAIVRELPGRIAPTRVSDVRPRVSGIIVERLFRQGSEVKAGDPLYRIDP
KPFEVEILSSRAALAKAEAVHERAVQQARRIALLFKERAAPEVENEKAISSEREATADIEARKADLARAQLNLDYATVRA
PIDGIVGAAQVSEGAIAVQNETSLVTIQQLDPIYADFTQSVSELNRLRRAFESGDLDQIAPDAIKVRLVLDDGTPYAIPG
KLLFSDAKVDAATGQVTLRGEFANPKRELLPGMYVRVRIEQGIDSDSVAVPQQAVQRNAAGGSEVYVIREDSRAIARPIR
TGPVQDGLVLITDGLRAGDKVVVDGFQKFAAGDKVAPQAWTEVSTATQLETKSASR

Specific function: May affect specific membrane functions, such as septum formation during cell division, and cell membrane permeability [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=370, Percent_Identity=38.6486486486486, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1789929, Length=356, Percent_Identity=37.9213483146067, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1786668, Length=364, Percent_Identity=40.1098901098901, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI87082036, Length=342, Percent_Identity=33.0409356725146, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1790519, Length=181, Percent_Identity=30.3867403314917, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40650; Mature: 40519

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGCNDTTPAQASANDQIAVPDVGIITVEQKSRAIVRELPGRIAPTRVSDVRPRVSGIIV
CCCCCCCCCCCCCCCCEEECCCCEEEEECCHHHHHHHHCCCCCCCCCHHHCCHHHHHHHH
ERLFRQGSEVKAGDPLYRIDPKPFEVEILSSRAALAKAEAVHERAVQQARRIALLFKERA
HHHHHCCCCCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APEVENEKAISSEREATADIEARKADLARAQLNLDYATVRAPIDGIVGAAQVSEGAIAVQ
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHEECCCEEEEE
NETSLVTIQQLDPIYADFTQSVSELNRLRRAFESGDLDQIAPDAIKVRLVLDDGTPYAIP
CCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEEEECCCCEECC
GKLLFSDAKVDAATGQVTLRGEFANPKRELLPGMYVRVRIEQGIDSDSVAVPQQAVQRNA
CCEEEECCCEECCCCEEEEEECCCCCHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHCC
AGGSEVYVIREDSRAIARPIRTGPVQDGLVLITDGLRAGDKVVVDGFQKFAAGDKVAPQA
CCCCEEEEEECCCHHHHCCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHCCCCCCCHH
WTEVSTATQLETKSASR
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AGCNDTTPAQASANDQIAVPDVGIITVEQKSRAIVRELPGRIAPTRVSDVRPRVSGIIV
CCCCCCCCCCCCCCCEEECCCCEEEEECCHHHHHHHHCCCCCCCCCHHHCCHHHHHHHH
ERLFRQGSEVKAGDPLYRIDPKPFEVEILSSRAALAKAEAVHERAVQQARRIALLFKERA
HHHHHCCCCCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APEVENEKAISSEREATADIEARKADLARAQLNLDYATVRAPIDGIVGAAQVSEGAIAVQ
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHEECCCEEEEE
NETSLVTIQQLDPIYADFTQSVSELNRLRRAFESGDLDQIAPDAIKVRLVLDDGTPYAIP
CCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEEEECCCCEECC
GKLLFSDAKVDAATGQVTLRGEFANPKRELLPGMYVRVRIEQGIDSDSVAVPQQAVQRNA
CCEEEECCCEECCCCEEEEEECCCCCHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHCC
AGGSEVYVIREDSRAIARPIRTGPVQDGLVLITDGLRAGDKVVVDGFQKFAAGDKVAPQA
CCCCEEEEEECCCHHHHCCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHCCCCCCCHH
WTEVSTATQLETKSASR
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1720861; 9278503 [H]