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The map label for this gene is tfdA [H]

Identifier: 146338184

GI number: 146338184

Start: 1149227

End: 1150114

Strand: Reverse

Name: tfdA [H]

Synonym: BRADO1082

Alternate gene names: 146338184

Gene position: 1150114-1149227 (Counterclockwise)

Preceding gene: 146338185

Following gene: 146338182

Centisome position: 15.42

GC content: 64.86

Gene sequence:

>888_bases
ATGGCCATCGCCATCCGGCAGCTACACCCCCATTTCGTCGGTGAAGTCTCGGGCGTGGATCTGCGCCATCCGCTCACGCG
CGATGAGGTCGCGGAGCTGCAGGCCGCGATCGATCACTACGCGGTGCTCGTCTTCCACGATCAGGACATCGGTGACGAGC
AGCAGCTCGCCTTTGCGCTGAATTTCGGCGACCGGGAGAAGGCGCGCGGCGGCACCGTGACGAAGAAGGAGGACTACCGT
CTCACCACCGGCCTCAACGACGTCTCCAATCTCGGCAAGGACGGCAAGCCGCTGCCGCCGGATCATCGCACGCATCTGTT
CAACCTCGGCAACTGCCTGTGGCACTCCGACAGCTCGTTCCGGCCGATCCCGGCGAAGTACTCGATCCTGTCGGCGCGCA
TCGTCAACCCGAAGGGTGGCAACACCGAATTCGCCGACATGCGTGCGGCCTACGACGCGCTGGACGAGCAGACCAAGGCC
GAGATCGATGATCTCGTCAGCGAACATTCGCTGATGTATTCGCGCGGCTCGCTCGGCTTCCTCGACTACACCGACGAGGA
GAAGAAGATGTTCAAGCCCGTCAGGCAGCGGCTGGTCCGCACGCATCCCGCCCACGGCCGCAAATCGCTGTATCTGTCAT
CGCATGCCGGCGCCGTGGTCGGCATGAGCATGCCGGAGGGACGGCTGCTGCTGCGCGATCTCACCGAGCACGCCACGCAG
CCGGAATTCGTCTACGTGCACAAATGGCGACTGCATGACCTGGTCATGTGGGACAACCGCCAGACCGTGCATCGCGTGCG
TCGCTACGATCAATCGCAGCCGCGCGACATGCGCCGGGCCACCGTGGCCGGGACGGAGCCGACGGTCGCGCAGCAGGCTG
CGGAGTAG

Upstream 100 bases:

>100_bases
CCCCGGTGCCTGCGTTGACTTCGCAGAGCGCCGGCGTAGCCTTTCGGGTGAGAAGCGGACCGACAGATCCGCCGCGACGA
GGACACGCAAGGAGAACGCC

Downstream 100 bases:

>100_bases
CGCTGGTTCAGCGACTTCGTTCCGGAGAGGCCGTCATTGCGAGGAGAGAAGCGACGAAGCCATCCAGAGGGGTCGCAGGG
CTCTGGATTGCTTCGCGGAG

Product: alpha-ketoglutarate-dependent 2, 4-dichlorophenoxyacetate dioxygenase

Products: Aminoacetaldehyde; CO2; H+; Sulfite; Succinate [C]

Alternate protein names: 2,4-D dioxygenase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAIAIRQLHPHFVGEVSGVDLRHPLTRDEVAELQAAIDHYAVLVFHDQDIGDEQQLAFALNFGDREKARGGTVTKKEDYR
LTTGLNDVSNLGKDGKPLPPDHRTHLFNLGNCLWHSDSSFRPIPAKYSILSARIVNPKGGNTEFADMRAAYDALDEQTKA
EIDDLVSEHSLMYSRGSLGFLDYTDEEKKMFKPVRQRLVRTHPAHGRKSLYLSSHAGAVVGMSMPEGRLLLRDLTEHATQ
PEFVYVHKWRLHDLVMWDNRQTVHRVRRYDQSQPRDMRRATVAGTEPTVAQQAAE

Sequences:

>Translated_295_residues
MAIAIRQLHPHFVGEVSGVDLRHPLTRDEVAELQAAIDHYAVLVFHDQDIGDEQQLAFALNFGDREKARGGTVTKKEDYR
LTTGLNDVSNLGKDGKPLPPDHRTHLFNLGNCLWHSDSSFRPIPAKYSILSARIVNPKGGNTEFADMRAAYDALDEQTKA
EIDDLVSEHSLMYSRGSLGFLDYTDEEKKMFKPVRQRLVRTHPAHGRKSLYLSSHAGAVVGMSMPEGRLLLRDLTEHATQ
PEFVYVHKWRLHDLVMWDNRQTVHRVRRYDQSQPRDMRRATVAGTEPTVAQQAAE
>Mature_294_residues
AIAIRQLHPHFVGEVSGVDLRHPLTRDEVAELQAAIDHYAVLVFHDQDIGDEQQLAFALNFGDREKARGGTVTKKEDYRL
TTGLNDVSNLGKDGKPLPPDHRTHLFNLGNCLWHSDSSFRPIPAKYSILSARIVNPKGGNTEFADMRAAYDALDEQTKAE
IDDLVSEHSLMYSRGSLGFLDYTDEEKKMFKPVRQRLVRTHPAHGRKSLYLSSHAGAVVGMSMPEGRLLLRDLTEHATQP
EFVYVHKWRLHDLVMWDNRQTVHRVRRYDQSQPRDMRRATVAGTEPTVAQQAAE

Specific function: Involved in degradation of the herbicide 2,4- dichlorophenoxyacetic acid (2,4-D). Is also able to degrade 2- methyl-4-chlorophenoxyacetic acid and 3-chlorobenzoic acid [H]

COG id: COG2175

COG function: function code Q; Probable taurine catabolism dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tfdA dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786565, Length=295, Percent_Identity=29.8305084745763, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003819 [H]

Pfam domain/function: PF02668 TauD [H]

EC number: 1.14.11.17 [C]

Molecular weight: Translated: 33471; Mature: 33340

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAIRQLHPHFVGEVSGVDLRHPLTRDEVAELQAAIDHYAVLVFHDQDIGDEQQLAFAL
CEEEEEHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEEE
NFGDREKARGGTVTKKEDYRLTTGLNDVSNLGKDGKPLPPDHRTHLFNLGNCLWHSDSSF
CCCCCHHCCCCCCCCCCCCEEECCCHHHHHCCCCCCCCCCCCHHHHHHCCCEEECCCCCC
RPIPAKYSILSARIVNPKGGNTEFADMRAAYDALDEQTKAEIDDLVSEHSLMYSRGSLGF
CCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LDYTDEEKKMFKPVRQRLVRTHPAHGRKSLYLSSHAGAVVGMSMPEGRLLLRDLTEHATQ
CCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHCCC
PEFVYVHKWRLHDLVMWDNRQTVHRVRRYDQSQPRDMRRATVAGTEPTVAQQAAE
CCEEEEEEEEEEEEEEECCHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure 
AIAIRQLHPHFVGEVSGVDLRHPLTRDEVAELQAAIDHYAVLVFHDQDIGDEQQLAFAL
EEEEEHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEEE
NFGDREKARGGTVTKKEDYRLTTGLNDVSNLGKDGKPLPPDHRTHLFNLGNCLWHSDSSF
CCCCCHHCCCCCCCCCCCCEEECCCHHHHHCCCCCCCCCCCCHHHHHHCCCEEECCCCCC
RPIPAKYSILSARIVNPKGGNTEFADMRAAYDALDEQTKAEIDDLVSEHSLMYSRGSLGF
CCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LDYTDEEKKMFKPVRQRLVRTHPAHGRKSLYLSSHAGAVVGMSMPEGRLLLRDLTEHATQ
CCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHCCC
PEFVYVHKWRLHDLVMWDNRQTVHRVRRYDQSQPRDMRRATVAGTEPTVAQQAAE
CCEEEEEEEEEEEEEEECCHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Ascorbate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Oxoglutarate; O2; Taurine [C]

Specific reaction: 2-Oxoglutarate + O2 + Taurine --> Aminoacetaldehyde + CO2 + H+ + Sulfite + Succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036764; 10777523; 11457355; 11955067 [H]