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The map label for this gene is cysH [H]

Identifier: 146338171

GI number: 146338171

Start: 1136703

End: 1137386

Strand: Direct

Name: cysH [H]

Synonym: BRADO1069

Alternate gene names: 146338171

Gene position: 1136703-1137386 (Clockwise)

Preceding gene: 146338170

Following gene: 146338172

Centisome position: 15.24

GC content: 67.98

Gene sequence:

>684_bases
GTGGCGGCGCTTGATGCGGCGCTGCGCAACGCAACGCCGCCCGAGGTCATCGAAGCGGCGCTTCAGGTGATCGGCCGCGA
TCGGCTCGCGGTGGTGTCGTCGTTCGGCTCGGAGTCGGCGGCGCTGCTGAAAGTCACCGCCGATGTCGATCCGGCGATTC
CCGTGGTCTTTCTCGACACCGGGCACATGTTTGCGGAGACGCTCGGCTATCGCGACCAGCTCATCGACATCCTCGGTCTG
CGCGACGTGCGCTCGATCGAGCCGGATGCCGCGGCGCTGGCGCGCGAGGATGCCGATAACGATCTATGGTTCTCCGATCC
CGACACCTGCTGCCGCATCCGCAAGGTGGAGCCGCTGGCCCGCGCGCTGGCGCCATTCACCGGATGGATCAACGGCCGCA
AGCGCTTCCAGGGTGGCCTGCGCGCGGCGATTGCCGTGGTCGAGCAGGACGGCGCGCGGATGAAGTTCAACCCGTTCGCC
AATGTCAGTCGCGAGGTTATCGCGGAGATCTATCGCTCGGCCAATCTGCCGCAGCATCCGCTGGTGGCCTCCGGCTTCCG
TTCGATCGGCTGCATGCCCTGCACCAGCCGCTCGGACGCGGGTGAGGACGAGCGCGCCGGCCGCTGGCGCGGTCGCGGCA
AGACAGAGTGCGGCATCCACACGATTGCGACACCGGCGGTGTAG

Upstream 100 bases:

>100_bases
GCCGCATCTCGGCGCTGCATCAGCGGCTGCAACAGCGTCACGCAGAGCGCGCCGGGCTATGAGCACGCTGGCCGCATCAC
ATCAGGCCGATGACACGTCC

Downstream 100 bases:

>100_bases
TTGGGCCCGGCGCGCGGGCTGCTCAGGCCGTCGTGACGACATTGTCTCGCGGCAAAGAACAGTCATCCCATCGCCGTGCA
TGACGTAGCAAAATGCCAAT

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MAALDAALRNATPPEVIEAALQVIGRDRLAVVSSFGSESAALLKVTADVDPAIPVVFLDTGHMFAETLGYRDQLIDILGL
RDVRSIEPDAAALAREDADNDLWFSDPDTCCRIRKVEPLARALAPFTGWINGRKRFQGGLRAAIAVVEQDGARMKFNPFA
NVSREVIAEIYRSANLPQHPLVASGFRSIGCMPCTSRSDAGEDERAGRWRGRGKTECGIHTIATPAV

Sequences:

>Translated_227_residues
MAALDAALRNATPPEVIEAALQVIGRDRLAVVSSFGSESAALLKVTADVDPAIPVVFLDTGHMFAETLGYRDQLIDILGL
RDVRSIEPDAAALAREDADNDLWFSDPDTCCRIRKVEPLARALAPFTGWINGRKRFQGGLRAAIAVVEQDGARMKFNPFA
NVSREVIAEIYRSANLPQHPLVASGFRSIGCMPCTSRSDAGEDERAGRWRGRGKTECGIHTIATPAV
>Mature_226_residues
AALDAALRNATPPEVIEAALQVIGRDRLAVVSSFGSESAALLKVTADVDPAIPVVFLDTGHMFAETLGYRDQLIDILGLR
DVRSIEPDAAALAREDADNDLWFSDPDTCCRIRKVEPLARALAPFTGWINGRKRFQGGLRAAIAVVEQDGARMKFNPFAN
VSREVIAEIYRSANLPQHPLVASGFRSIGCMPCTSRSDAGEDERAGRWRGRGKTECGIHTIATPAV

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=193, Percent_Identity=31.6062176165803, Blast_Score=91, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6325425, Length=230, Percent_Identity=29.5652173913043, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 24558; Mature: 24427

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALDAALRNATPPEVIEAALQVIGRDRLAVVSSFGSESAALLKVTADVDPAIPVVFLDT
CCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEC
GHMFAETLGYRDQLIDILGLRDVRSIEPDAAALAREDADNDLWFSDPDTCCRIRKVEPLA
CHHHHHHHCCHHHHHHHHCCHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
RALAPFTGWINGRKRFQGGLRAAIAVVEQDGARMKFNPFANVSREVIAEIYRSANLPQHP
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCC
LVASGFRSIGCMPCTSRSDAGEDERAGRWRGRGKTECGIHTIATPAV
CHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure 
AALDAALRNATPPEVIEAALQVIGRDRLAVVSSFGSESAALLKVTADVDPAIPVVFLDT
CCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEC
GHMFAETLGYRDQLIDILGLRDVRSIEPDAAALAREDADNDLWFSDPDTCCRIRKVEPLA
CHHHHHHHCCHHHHHHHHCCHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
RALAPFTGWINGRKRFQGGLRAAIAVVEQDGARMKFNPFANVSREVIAEIYRSANLPQHP
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCC
LVASGFRSIGCMPCTSRSDAGEDERAGRWRGRGKTECGIHTIATPAV
CHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]