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The map label for this gene is glcB

Identifier: 146338162

GI number: 146338162

Start: 1123808

End: 1125982

Strand: Reverse

Name: glcB

Synonym: BRADO1059

Alternate gene names: 146338162

Gene position: 1125982-1123808 (Counterclockwise)

Preceding gene: 146338163

Following gene: 146338160

Centisome position: 15.1

GC content: 65.06

Gene sequence:

>2175_bases
ATGACCCGTATCGACGCCCATGGACTGAAGATCGCCCCCGTCCTGTTCGACTTCATCACCAAGGAGGCGGCGCCGAAGAC
CGGGATCGATCCGGACGCGTTCTGGGCCGGCGTGGCCGCGATCCTGCGCGATCTCGGGTCCAAAAACCGCGAATTGCTCA
AATTCCGCGATACGCTGCAGGACAAGATCGACGCCTGGCACCTCGCCAACAAGGGCAAGGCGTTCGACCTCGGCGCCTAC
ACGGCGTTCTTGAAGGAGATCGGCTACCTCCTGCCGGAGCCCGCGACCCAGAAGGTCGAGACGGCCAACGTTGACGACGA
GATCGGCAAGATCTGCGGTCCCCAACTCGTGGTGCCGCTGACCAACGCCCGCTACGCGCTGAATGCGGCCAATGCGCGCT
GGGGTTCGCTGTACGACGCCTTCTACGGCACCGACGCCATCCCGCATGATCCGAGCGAGGGCGGAAAGGGCTATAACAAG
GCCAGGGGCGACAAGGTGATCGCCAAGGCCAAGGCGTTCCTCGACGCCGCCGCGCCGCTCGCCACCGGCAGCCACACCGA
CGTCACCGCCTACAGCGTCATTGCTGGGCAACTGTCCGCCAAGCTGAAGAGCGGCAACGCCACTGCACTGAAGAACGCCG
GTCAGTTCGCCGGCTACCAGGGCTCGGCCGAGGCGCCGTCCGTGATCCTGCTCGCCAACAATGGCCTGCATGTCGAGGTC
GTGATCGACCGCAGCCACGTCATCGGCAAGGACGACCCGGCCGGCGTCTCCGACATGATCCTGGAAGCCGCGGTCTCGAC
CATCCTCGACATGGAGGACTCGGTCGCCGCCGTCGACGCCGAGGACAAGGTGCTGGTCTATCGCAACACGCTCGGCCTGA
TGAACGGCACGCTGTCGGCCGATTTCGAGAAGGGCGGCAAGACGCTCACGCGCGCCTTGAACAGCGACCGCAGCTACACG
ACGCCGTCCGGCGGCACGCTCGCTTTACACGGCCGCAGCCTGCTGCTGATCCGCAACTGCGGACATCACATGTTCACCGA
CGCCGTGCTCGGCGCCGACGGCGAGGAAATTCCGGAGGGCATTCTCGACGCCGCCGTCACCGGCCTGCTGGCGATCCACG
ACCTCAAGGGCCACTCCAAGGTCAAGAACAGCCGCACCGGCTCGATTTACATCGTCAAGCCGAAGATGCACGGCCCCGAC
GAGGTCGCGCTGACGGTCGAGCTGTTCGCGCGCGTCGAAAAGATGCTCGGCCTGCCGGAGAACACCATGAAGGTCGGCAT
CATGGACGAGGAGCGCCGCACCACGGTCAACCTCAAGGCCTGCATCCAGCAGGCCTCCAAGCGCATCGTGTTCATCAACA
CCGGCTTCCTCGACCGCACCGGCGACGAGATCCACACCTCGATGGAAGCGGGTCCGATGATCCGCAAGAACGAGATGAAG
GCGCAGCCCTGGATCAAGGCCTATGAGGACTGGAACGTCGACATGGGCCTGATCGACGGCCTGCCCGGCCACGCCCAGAT
CGGCAAGGGCATGTGGGCGGCGCCGGACAAGATGGCGGACATGCTGGCGCAGAAGATCGGCCACCCCTCCGCCGGCGCCA
CCACTGCCTGGGTGCCGTCGCCGACGGCGGCGACCTTGCATGCCCTGCACTATCACCAGGTCAATGTGAAGGCTCGGCAG
GACGAACTGACAAAGGGCGGGCCGCGCGCCAAGCTCGGCGACATCCTCACCATCCCCGTGTCGAACTCGAACTGGGCGCC
GGACGACGTCCGCCAGGAGATCGACAACAACTGCCAGGGTATTCTCGGCTACGTCGTGCGCTGGATCGACCAGGGCGTCG
GCTGCTCCAAGGTGCCTGATATCCACGATGTCGGCCTGATGGAAGACCGCGCAACGTTGCGCATCTCCAGCCAGCATCTC
GCCAACTGGCTGCATCATGGCGTGGTCAGCAAGGACCAGGTGATGGATTCGCTGAAGCGCATGGCGGTGGTGGTGGACAA
GCAGAACGCCGGCGATCCGCTGTATCAGCCGATGGCACCATCGTTCGACGGAGTGGCGTTCAAGGCGGCCTGCGACCTGG
TGTTCGAAGGCCGGACGCAGCCGAATGGCTATACCGAGTACATCCTCACCGCGCGGCGGCGCGAAGCCAAAGCCAGCGAG
ACGGCGAAGGCCTGA

Upstream 100 bases:

>100_bases
CGGCTTTCACGTTAAATGCACTGCACAAGCCGCCGGGAGGCGGCCGTTTCAGCATGCCCAAGACACGCGCAACGCCCTTA
TTCGAGAGAAACGTCCCATC

Downstream 100 bases:

>100_bases
CGACGAAAAGCCCCGGCCGACCGCCGGGGCTTTTTTAGAGCGAATAAAGGTATCATGAAACCTTTTCAGATGACGGCGAT
GTATTTCAGCAGCGAGATCA

Product: malate synthase G

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 724; Mature: 723

Protein sequence:

>724_residues
MTRIDAHGLKIAPVLFDFITKEAAPKTGIDPDAFWAGVAAILRDLGSKNRELLKFRDTLQDKIDAWHLANKGKAFDLGAY
TAFLKEIGYLLPEPATQKVETANVDDEIGKICGPQLVVPLTNARYALNAANARWGSLYDAFYGTDAIPHDPSEGGKGYNK
ARGDKVIAKAKAFLDAAAPLATGSHTDVTAYSVIAGQLSAKLKSGNATALKNAGQFAGYQGSAEAPSVILLANNGLHVEV
VIDRSHVIGKDDPAGVSDMILEAAVSTILDMEDSVAAVDAEDKVLVYRNTLGLMNGTLSADFEKGGKTLTRALNSDRSYT
TPSGGTLALHGRSLLLIRNCGHHMFTDAVLGADGEEIPEGILDAAVTGLLAIHDLKGHSKVKNSRTGSIYIVKPKMHGPD
EVALTVELFARVEKMLGLPENTMKVGIMDEERRTTVNLKACIQQASKRIVFINTGFLDRTGDEIHTSMEAGPMIRKNEMK
AQPWIKAYEDWNVDMGLIDGLPGHAQIGKGMWAAPDKMADMLAQKIGHPSAGATTAWVPSPTAATLHALHYHQVNVKARQ
DELTKGGPRAKLGDILTIPVSNSNWAPDDVRQEIDNNCQGILGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHL
ANWLHHGVVSKDQVMDSLKRMAVVVDKQNAGDPLYQPMAPSFDGVAFKAACDLVFEGRTQPNGYTEYILTARRREAKASE
TAKA

Sequences:

>Translated_724_residues
MTRIDAHGLKIAPVLFDFITKEAAPKTGIDPDAFWAGVAAILRDLGSKNRELLKFRDTLQDKIDAWHLANKGKAFDLGAY
TAFLKEIGYLLPEPATQKVETANVDDEIGKICGPQLVVPLTNARYALNAANARWGSLYDAFYGTDAIPHDPSEGGKGYNK
ARGDKVIAKAKAFLDAAAPLATGSHTDVTAYSVIAGQLSAKLKSGNATALKNAGQFAGYQGSAEAPSVILLANNGLHVEV
VIDRSHVIGKDDPAGVSDMILEAAVSTILDMEDSVAAVDAEDKVLVYRNTLGLMNGTLSADFEKGGKTLTRALNSDRSYT
TPSGGTLALHGRSLLLIRNCGHHMFTDAVLGADGEEIPEGILDAAVTGLLAIHDLKGHSKVKNSRTGSIYIVKPKMHGPD
EVALTVELFARVEKMLGLPENTMKVGIMDEERRTTVNLKACIQQASKRIVFINTGFLDRTGDEIHTSMEAGPMIRKNEMK
AQPWIKAYEDWNVDMGLIDGLPGHAQIGKGMWAAPDKMADMLAQKIGHPSAGATTAWVPSPTAATLHALHYHQVNVKARQ
DELTKGGPRAKLGDILTIPVSNSNWAPDDVRQEIDNNCQGILGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHL
ANWLHHGVVSKDQVMDSLKRMAVVVDKQNAGDPLYQPMAPSFDGVAFKAACDLVFEGRTQPNGYTEYILTARRREAKASE
TAKA
>Mature_723_residues
TRIDAHGLKIAPVLFDFITKEAAPKTGIDPDAFWAGVAAILRDLGSKNRELLKFRDTLQDKIDAWHLANKGKAFDLGAYT
AFLKEIGYLLPEPATQKVETANVDDEIGKICGPQLVVPLTNARYALNAANARWGSLYDAFYGTDAIPHDPSEGGKGYNKA
RGDKVIAKAKAFLDAAAPLATGSHTDVTAYSVIAGQLSAKLKSGNATALKNAGQFAGYQGSAEAPSVILLANNGLHVEVV
IDRSHVIGKDDPAGVSDMILEAAVSTILDMEDSVAAVDAEDKVLVYRNTLGLMNGTLSADFEKGGKTLTRALNSDRSYTT
PSGGTLALHGRSLLLIRNCGHHMFTDAVLGADGEEIPEGILDAAVTGLLAIHDLKGHSKVKNSRTGSIYIVKPKMHGPDE
VALTVELFARVEKMLGLPENTMKVGIMDEERRTTVNLKACIQQASKRIVFINTGFLDRTGDEIHTSMEAGPMIRKNEMKA
QPWIKAYEDWNVDMGLIDGLPGHAQIGKGMWAAPDKMADMLAQKIGHPSAGATTAWVPSPTAATLHALHYHQVNVKARQD
ELTKGGPRAKLGDILTIPVSNSNWAPDDVRQEIDNNCQGILGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHLA
NWLHHGVVSKDQVMDSLKRMAVVVDKQNAGDPLYQPMAPSFDGVAFKAACDLVFEGRTQPNGYTEYILTARRREAKASET
AKA

Specific function: Accounts For Almost The Entire Malate-Synthesizing Activity In Cells Metabolizing Glyoxylate. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family. GlcB subfamily

Homologues:

Organism=Escherichia coli, GI1789348, Length=719, Percent_Identity=59.2489568845619, Blast_Score=867, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MASZ_BRASO (A4YM47)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001203210.1
- ProteinModelPortal:   A4YM47
- SMR:   A4YM47
- STRING:   A4YM47
- GeneID:   5114900
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO1059
- eggNOG:   COG2225
- HOGENOM:   HBG350005
- OMA:   PKMHGPD
- ProtClustDB:   PRK02999
- BioCyc:   BSP376:BRADO1059-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00641
- InterPro:   IPR011076
- InterPro:   IPR001465
- InterPro:   IPR006253
- PANTHER:   PTHR21631:SF1
- TIGRFAMs:   TIGR01345

Pfam domain/function: PF01274 Malate_synthase; SSF51645 Malat_synth_like

EC number: =2.3.3.9

Molecular weight: Translated: 78075; Mature: 77944

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: ACT_SITE 338-338 ACT_SITE 629-629

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIDAHGLKIAPVLFDFITKEAAPKTGIDPDAFWAGVAAILRDLGSKNRELLKFRDTLQ
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DKIDAWHLANKGKAFDLGAYTAFLKEIGYLLPEPATQKVETANVDDEIGKICGPQLVVPL
HHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCEEEEEE
TNARYALNAANARWGSLYDAFYGTDAIPHDPSEGGKGYNKARGDKVIAKAKAFLDAAAPL
CCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCC
ATGSHTDVTAYSVIAGQLSAKLKSGNATALKNAGQFAGYQGSAEAPSVILLANNGLHVEV
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEE
VIDRSHVIGKDDPAGVSDMILEAAVSTILDMEDSVAAVDAEDKVLVYRNTLGLMNGTLSA
EEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCHHHHCCCCCC
DFEKGGKTLTRALNSDRSYTTPSGGTLALHGRSLLLIRNCGHHMFTDAVLGADGEEIPEG
CHHHCHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHCCCHHHHHHH
ILDAAVTGLLAIHDLKGHSKVKNSRTGSIYIVKPKMHGPDEVALTVELFARVEKMLGLPE
HHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCC
NTMKVGIMDEERRTTVNLKACIQQASKRIVFINTGFLDRTGDEIHTSMEAGPMIRKNEMK
CCEEEEEEECCCHHEEHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHCCCCEECCCCC
AQPWIKAYEDWNVDMGLIDGLPGHAQIGKGMWAAPDKMADMLAQKIGHPSAGATTAWVPS
CCCCHHHHCCCCCCCHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCC
PTAATLHALHYHQVNVKARQDELTKGGPRAKLGDILTIPVSNSNWAPDDVRQEIDNNCQG
CCHHHHHHHHEEEEECEECHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHH
ILGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHLANWLHHGVVSKDQVMDSLKR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHH
MAVVVDKQNAGDPLYQPMAPSFDGVAFKAACDLVFEGRTQPNGYTEYILTARRREAKASE
HEEEEECCCCCCCCCCCCCCCCCCEEHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
TAKA
CCCC
>Mature Secondary Structure 
TRIDAHGLKIAPVLFDFITKEAAPKTGIDPDAFWAGVAAILRDLGSKNRELLKFRDTLQ
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DKIDAWHLANKGKAFDLGAYTAFLKEIGYLLPEPATQKVETANVDDEIGKICGPQLVVPL
HHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCEEEEEE
TNARYALNAANARWGSLYDAFYGTDAIPHDPSEGGKGYNKARGDKVIAKAKAFLDAAAPL
CCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCC
ATGSHTDVTAYSVIAGQLSAKLKSGNATALKNAGQFAGYQGSAEAPSVILLANNGLHVEV
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEE
VIDRSHVIGKDDPAGVSDMILEAAVSTILDMEDSVAAVDAEDKVLVYRNTLGLMNGTLSA
EEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCHHHHCCCCCC
DFEKGGKTLTRALNSDRSYTTPSGGTLALHGRSLLLIRNCGHHMFTDAVLGADGEEIPEG
CHHHCHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHCCCHHHHHHH
ILDAAVTGLLAIHDLKGHSKVKNSRTGSIYIVKPKMHGPDEVALTVELFARVEKMLGLPE
HHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCC
NTMKVGIMDEERRTTVNLKACIQQASKRIVFINTGFLDRTGDEIHTSMEAGPMIRKNEMK
CCEEEEEEECCCHHEEHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHCCCCEECCCCC
AQPWIKAYEDWNVDMGLIDGLPGHAQIGKGMWAAPDKMADMLAQKIGHPSAGATTAWVPS
CCCCHHHHCCCCCCCHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCC
PTAATLHALHYHQVNVKARQDELTKGGPRAKLGDILTIPVSNSNWAPDDVRQEIDNNCQG
CCHHHHHHHHEEEEECEECHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHH
ILGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHLANWLHHGVVSKDQVMDSLKR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHH
MAVVVDKQNAGDPLYQPMAPSFDGVAFKAACDLVFEGRTQPNGYTEYILTARRREAKASE
HEEEEECCCCCCCCCCCCCCCCCCEEHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
TAKA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA