Click here to switch to the map view.

The map label for this gene is rpoE [H]

Identifier: 146338150

GI number: 146338150

Start: 1114429

End: 1114977

Strand: Reverse

Name: rpoE [H]

Synonym: BRADO1047

Alternate gene names: 146338150

Gene position: 1114977-1114429 (Counterclockwise)

Preceding gene: 146338151

Following gene: 146338133

Centisome position: 14.95

GC content: 61.93

Gene sequence:

>549_bases
ATGCCTCTCACTGATTCCTTGCGGGACGATATCCTTGCGGCCGTCCCAAGCCTCCGCGCATTCGCGATTTCCTTGAGTGG
TAACAGCGATCGCGCCGACGACCTCGTGCAGGAAACTCTGCTGCGGGCGCTTGCCAACATCGATTCGTTTCAGCCGGGCT
CGAATCTGCCCGCGTGGTTGTTTACGATTTTGAGAAATCTGTTCCGCTCCGATTACCGCAAACGACGGCGCGAAGTCGAG
GACGCCGAGGGCAACTACGCCAAGACGCTCAAGACGCATCCCTCCCAGGGGGCGCATCTGGAGTTCGAAGAGTTCCGTGC
GGCTCTCGAGAAGCTTCCTCAGGACCAGCGCGAAGCGCTGATTCTGGTCGGCGCGTCTGGCTTCTCCTATGAAGATGCGG
CGGCGATCTGCGGCTGCGCCGTGGGCACGATCAAGAGCCGTGTTAACCGCGCGCGCTCCAAACTCGCGGCGCTGCTTTAT
GTCGAAGGTGCCGAGGATTTCGGCCCTGACGAGACGGTGCGGGCTGTGATCGGCGGCAGCGGCGGCTGA

Upstream 100 bases:

>100_bases
CCGGTTTGCAGAATTGGTGCGCAAACTTGACGCACCGGCAGCACAGTCCCACGTGGAGAACGGCGGGGGATCCAACGACC
AAAACGACGGGAGGGAGTGA

Downstream 100 bases:

>100_bases
CGCCACTACCGCATTCTGTAAAAAGAGAAGGAGGCGGCCTCCTCGCGGCGGCCGCCTCTTTTGCGTTGGAAGAACGAGTA
CAAAGAAGGAGTCACGCCGT

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MPLTDSLRDDILAAVPSLRAFAISLSGNSDRADDLVQETLLRALANIDSFQPGSNLPAWLFTILRNLFRSDYRKRRREVE
DAEGNYAKTLKTHPSQGAHLEFEEFRAALEKLPQDQREALILVGASGFSYEDAAAICGCAVGTIKSRVNRARSKLAALLY
VEGAEDFGPDETVRAVIGGSGG

Sequences:

>Translated_182_residues
MPLTDSLRDDILAAVPSLRAFAISLSGNSDRADDLVQETLLRALANIDSFQPGSNLPAWLFTILRNLFRSDYRKRRREVE
DAEGNYAKTLKTHPSQGAHLEFEEFRAALEKLPQDQREALILVGASGFSYEDAAAICGCAVGTIKSRVNRARSKLAALLY
VEGAEDFGPDETVRAVIGGSGG
>Mature_181_residues
PLTDSLRDDILAAVPSLRAFAISLSGNSDRADDLVQETLLRALANIDSFQPGSNLPAWLFTILRNLFRSDYRKRRREVED
AEGNYAKTLKTHPSQGAHLEFEEFRAALEKLPQDQREALILVGASGFSYEDAAAICGCAVGTIKSRVNRARSKLAALLYV
EGAEDFGPDETVRAVIGGSGG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=137, Percent_Identity=34.3065693430657, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 19812; Mature: 19681

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTDSLRDDILAAVPSLRAFAISLSGNSDRADDLVQETLLRALANIDSFQPGSNLPAWL
CCCCHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
FTILRNLFRSDYRKRRREVEDAEGNYAKTLKTHPSQGAHLEFEEFRAALEKLPQDQREAL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEE
ILVGASGFSYEDAAAICGCAVGTIKSRVNRARSKLAALLYVEGAEDFGPDETVRAVIGGS
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
GG
CC
>Mature Secondary Structure 
PLTDSLRDDILAAVPSLRAFAISLSGNSDRADDLVQETLLRALANIDSFQPGSNLPAWL
CCCHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
FTILRNLFRSDYRKRRREVEDAEGNYAKTLKTHPSQGAHLEFEEFRAALEKLPQDQREAL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEE
ILVGASGFSYEDAAAICGCAVGTIKSRVNRARSKLAALLYVEGAEDFGPDETVRAVIGGS
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
GG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]