Click here to switch to the map view.

The map label for this gene is glpK

Identifier: 146338091

GI number: 146338091

Start: 1049159

End: 1050661

Strand: Direct

Name: glpK

Synonym: BRADO0987

Alternate gene names: 146338091

Gene position: 1049159-1050661 (Clockwise)

Preceding gene: 146338090

Following gene: 146338093

Centisome position: 14.07

GC content: 68.86

Gene sequence:

>1503_bases
ATGTCCTTCGTGCTGGCCATCGATCAGGGCACCACGTCCTCGCGCGCGATGGTGTTCCGCTCCGACATCTCGATCGCCGC
GGTGGCGCAGCAGGAATTCCCGCAGCATTTCCCGGCCTCGGGCTGGGTCGAGCACGAGCCGGAGGACATCTGGACCTCGA
CCGTGATGACCTGCCGCGACGCGCTGGAGAAGGCGGGGCTCGCGGCCAAGGACATCGCCGCGATCGGCATCACCAATCAG
CGCGAGACCACCGTGGTGTGGGACCGCGCCACCGGACAGGCGGTGCATCGCGCCATCGTCTGGCAGGACCGCCGCACCGC
CGATATCTGCGCGAAGCTCAAGGCCGAGGGCCACGAGCCCGACGTCAGCGCCCGGACCGGCCTGATCATCGATCCCTATT
TCTCCGGCACCAAGGTCGCCTGGATCCTCGACCATGTGCCCGGCGCGCGCGAGCGCGCCGAGCGCGGCGAGCTCCTGTTC
GGCACCGTCGACTGCTATCTGCTGTGGCGGCTGACCGGCGGCCGCGTGCACGCGACCGACGCCACCAACGCCTCGCGCAC
CCTGCTGTTCAACATCCACACCGGCCAGTGGGACGACACGCTGCTGAAGCTGCTGCGCGTGCCGCGTTCGATGCTGCCCG
AGGTGAAGGATTCCTCCGCCGATTTCGGCACCACCACGCCCGATTTGTTCGGCGGCCCGATCAAGGTGGCGGGCATCGCC
GGCGACCAGCAGGCGGCGACGATCGGCCAGGCCTGCTTCACGCCGGGCATGATGAAGTCGACCTACGGCACCGGCTGCTT
CGCGCTGCTCAACACCGGCGCGACGCCGGTGAAGTCGAACAACAAGCTGCTGACGACCATCGCCTATCAGCTCAACGGTA
TCAGAACCTACGCGCTCGAAGGCTCGATCTTCGTCGCCGGCTCCGCGGTGCAGTGGCTGCGCGACGGGCTCGGAATCATC
AAGCACGCCGCCGAGACCGGCCCGCTTGCCGATAAATCCGATTCGATGCAGTCGGTCTATCTGGTGCCGGCCTTCGTCGG
CCTGGGCGCACCCTATTGGAATCCGCGCGTGCGCGGCGCGCTGTTCGGCCTCACCCGCAACACCGGCCCTGCCGAGCTCG
CCCATGCGACGCTCGAAAGCGTCTGCTACCAGACCTACGACCTCTGGGCGGCGATGCGCGCCGACTGGCCGGATGCGTCG
GCCGCGACCATCGTGCTGCGCGTCGACGGCGGCATGACGGCGTCGGACTGGACCATGCAGCGCCTCGCCGATCTGCTCGA
CGCGCCGGTCGACCGCCCGATGATCCAGGAGACGACGGCGCTCGGCGCCGCCTATCTCGCCGGCCTGAATGCGGGCGTCT
ATCCGGAGCCGGAGAAATTCGCCGACAATTGGCGCCTCGAGCACCGCTTCAGGCCGGCGATGAGCGCGGCCACGCGGCAG
CGCAAGCTCGCGGGCTGGGCGCGCGCGGTGAAGGGCGTGCTGGCAAGCGACGAAGGCGAGTAG

Upstream 100 bases:

>100_bases
GGTGAAGCGGCGCACCTTGCGCACCCTCCAACCACCTTGACCTTCCCCCGCCAATCCGCTTGCTTGTCGGCCCAACGTCA
ACCTTCCGGGAAGAAGCCCC

Downstream 100 bases:

>100_bases
GGCACGGCGCCCGCAGCGTCGGTCAGCTCGCGAAGGCGGCCAGGTCGGCATTGATCTGCGCCATATGGGTCACGAACAAT
CCGTGCGGTCCGCCAGGATA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSFVLAIDQGTTSSRAMVFRSDISIAAVAQQEFPQHFPASGWVEHEPEDIWTSTVMTCRDALEKAGLAAKDIAAIGITNQ
RETTVVWDRATGQAVHRAIVWQDRRTADICAKLKAEGHEPDVSARTGLIIDPYFSGTKVAWILDHVPGARERAERGELLF
GTVDCYLLWRLTGGRVHATDATNASRTLLFNIHTGQWDDTLLKLLRVPRSMLPEVKDSSADFGTTTPDLFGGPIKVAGIA
GDQQAATIGQACFTPGMMKSTYGTGCFALLNTGATPVKSNNKLLTTIAYQLNGIRTYALEGSIFVAGSAVQWLRDGLGII
KHAAETGPLADKSDSMQSVYLVPAFVGLGAPYWNPRVRGALFGLTRNTGPAELAHATLESVCYQTYDLWAAMRADWPDAS
AATIVLRVDGGMTASDWTMQRLADLLDAPVDRPMIQETTALGAAYLAGLNAGVYPEPEKFADNWRLEHRFRPAMSAATRQ
RKLAGWARAVKGVLASDEGE

Sequences:

>Translated_500_residues
MSFVLAIDQGTTSSRAMVFRSDISIAAVAQQEFPQHFPASGWVEHEPEDIWTSTVMTCRDALEKAGLAAKDIAAIGITNQ
RETTVVWDRATGQAVHRAIVWQDRRTADICAKLKAEGHEPDVSARTGLIIDPYFSGTKVAWILDHVPGARERAERGELLF
GTVDCYLLWRLTGGRVHATDATNASRTLLFNIHTGQWDDTLLKLLRVPRSMLPEVKDSSADFGTTTPDLFGGPIKVAGIA
GDQQAATIGQACFTPGMMKSTYGTGCFALLNTGATPVKSNNKLLTTIAYQLNGIRTYALEGSIFVAGSAVQWLRDGLGII
KHAAETGPLADKSDSMQSVYLVPAFVGLGAPYWNPRVRGALFGLTRNTGPAELAHATLESVCYQTYDLWAAMRADWPDAS
AATIVLRVDGGMTASDWTMQRLADLLDAPVDRPMIQETTALGAAYLAGLNAGVYPEPEKFADNWRLEHRFRPAMSAATRQ
RKLAGWARAVKGVLASDEGE
>Mature_499_residues
SFVLAIDQGTTSSRAMVFRSDISIAAVAQQEFPQHFPASGWVEHEPEDIWTSTVMTCRDALEKAGLAAKDIAAIGITNQR
ETTVVWDRATGQAVHRAIVWQDRRTADICAKLKAEGHEPDVSARTGLIIDPYFSGTKVAWILDHVPGARERAERGELLFG
TVDCYLLWRLTGGRVHATDATNASRTLLFNIHTGQWDDTLLKLLRVPRSMLPEVKDSSADFGTTTPDLFGGPIKVAGIAG
DQQAATIGQACFTPGMMKSTYGTGCFALLNTGATPVKSNNKLLTTIAYQLNGIRTYALEGSIFVAGSAVQWLRDGLGIIK
HAAETGPLADKSDSMQSVYLVPAFVGLGAPYWNPRVRGALFGLTRNTGPAELAHATLESVCYQTYDLWAAMRADWPDASA
ATIVLRVDGGMTASDWTMQRLADLLDAPVDRPMIQETTALGAAYLAGLNAGVYPEPEKFADNWRLEHRFRPAMSAATRQR
KLAGWARAVKGVLASDEGE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=43.6254980079681, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=43.6254980079681, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI42794763, Length=509, Percent_Identity=43.614931237721, Blast_Score=371, Evalue=1e-102,
Organism=Homo sapiens, GI41393575, Length=503, Percent_Identity=43.1411530815109, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI88196792, Length=505, Percent_Identity=29.9009900990099, Blast_Score=268, Evalue=1e-71,
Organism=Escherichia coli, GI1790361, Length=498, Percent_Identity=53.4136546184739, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1789987, Length=464, Percent_Identity=25, Blast_Score=115, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17535599, Length=501, Percent_Identity=38.12375249501, Blast_Score=324, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI71986140, Length=519, Percent_Identity=23.3140655105973, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71986147, Length=299, Percent_Identity=25.4180602006689, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321755, Length=478, Percent_Identity=35.3556485355649, Blast_Score=259, Evalue=7e-70,
Organism=Drosophila melanogaster, GI17864214, Length=503, Percent_Identity=41.1530815109344, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24654573, Length=443, Percent_Identity=41.5349887133183, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24655555, Length=506, Percent_Identity=36.3636363636364, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24655560, Length=506, Percent_Identity=36.3636363636364, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24655570, Length=506, Percent_Identity=36.3636363636364, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24655574, Length=506, Percent_Identity=36.3636363636364, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24655565, Length=506, Percent_Identity=36.3636363636364, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI19922058, Length=534, Percent_Identity=33.3333333333333, Blast_Score=262, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24652871, Length=495, Percent_Identity=34.5454545454545, Blast_Score=253, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24656367, Length=511, Percent_Identity=27.7886497064579, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21357867, Length=527, Percent_Identity=26.9449715370019, Blast_Score=172, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24656372, Length=358, Percent_Identity=28.2122905027933, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45552909, Length=255, Percent_Identity=29.0196078431373, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_BRASO (A4YLX6)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001203139.1
- ProteinModelPortal:   A4YLX6
- STRING:   A4YLX6
- GeneID:   5117577
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO0987
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   GWVEHEP
- ProtClustDB:   CLSK818559
- BioCyc:   BSP376:BRADO0987-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54185; Mature: 54054

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 11-11 BINDING 15-15 BINDING 81-81 BINDING 133-133 BINDING 242-242 BINDING 264-264 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVLAIDQGTTSSRAMVFRSDISIAAVAQQEFPQHFPASGWVEHEPEDIWTSTVMTCRD
CEEEEEECCCCCCCCEEEEECCCCEEEHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
ALEKAGLAAKDIAAIGITNQRETTVVWDRATGQAVHRAIVWQDRRTADICAKLKAEGHEP
HHHHCCCCHHHEEEEECCCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCC
DVSARTGLIIDPYFSGTKVAWILDHVPGARERAERGELLFGTVDCYLLWRLTGGRVHATD
CCCCCCCEEECCCCCCCEEEEEEECCCCHHHHHHCCCEEEEEEEEEEEEEECCCEEEECC
ATNASRTLLFNIHTGQWDDTLLKLLRVPRSMLPEVKDSSADFGTTTPDLFGGPIKVAGIA
CCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHCCCCEEEEEEC
GDQQAATIGQACFTPGMMKSTYGTGCFALLNTGATPVKSNNKLLTTIAYQLNGIRTYALE
CCCHHHHHHHHHCCCCHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEEHHCCEEEEEEC
GSIFVAGSAVQWLRDGLGIIKHAAETGPLADKSDSMQSVYLVPAFVGLGAPYWNPRVRGA
CCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCEE
LFGLTRNTGPAELAHATLESVCYQTYDLWAAMRADWPDASAATIVLRVDGGMTASDWTMQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHH
RLADLLDAPVDRPMIQETTALGAAYLAGLNAGVYPEPEKFADNWRLEHRFRPAMSAATRQ
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHCHHHHHHHHH
RKLAGWARAVKGVLASDEGE
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SFVLAIDQGTTSSRAMVFRSDISIAAVAQQEFPQHFPASGWVEHEPEDIWTSTVMTCRD
EEEEEECCCCCCCCEEEEECCCCEEEHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
ALEKAGLAAKDIAAIGITNQRETTVVWDRATGQAVHRAIVWQDRRTADICAKLKAEGHEP
HHHHCCCCHHHEEEEECCCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCC
DVSARTGLIIDPYFSGTKVAWILDHVPGARERAERGELLFGTVDCYLLWRLTGGRVHATD
CCCCCCCEEECCCCCCCEEEEEEECCCCHHHHHHCCCEEEEEEEEEEEEEECCCEEEECC
ATNASRTLLFNIHTGQWDDTLLKLLRVPRSMLPEVKDSSADFGTTTPDLFGGPIKVAGIA
CCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHCCCCEEEEEEC
GDQQAATIGQACFTPGMMKSTYGTGCFALLNTGATPVKSNNKLLTTIAYQLNGIRTYALE
CCCHHHHHHHHHCCCCHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEEHHCCEEEEEEC
GSIFVAGSAVQWLRDGLGIIKHAAETGPLADKSDSMQSVYLVPAFVGLGAPYWNPRVRGA
CCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCEE
LFGLTRNTGPAELAHATLESVCYQTYDLWAAMRADWPDASAATIVLRVDGGMTASDWTMQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHH
RLADLLDAPVDRPMIQETTALGAAYLAGLNAGVYPEPEKFADNWRLEHRFRPAMSAATRQ
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHCHHHHHHHHH
RKLAGWARAVKGVLASDEGE
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA