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The map label for this gene is gatA [H]

Identifier: 146337976

GI number: 146337976

Start: 920427

End: 921785

Strand: Reverse

Name: gatA [H]

Synonym: BRADO0868

Alternate gene names: 146337976

Gene position: 921785-920427 (Counterclockwise)

Preceding gene: 146337977

Following gene: 146337972

Centisome position: 12.36

GC content: 68.87

Gene sequence:

>1359_bases
TTGATGCGCGCTGATGAGATCGCAGGGACCGTCCTGGCAAGGCAGAGATCGGCACGGGAAATGGCCGAGCGGGCGCTGAC
GCGCATCGATGCGGCGCGCGCGCTCAACGCCGTCGTGACGATCGATCCGGAGCGAACGCGCGCGGAGGCCGACGCCGTCG
ATGCCCGCGTGGCAGCGGGTGAGGCGCTGCCGCTCGCTGGTGTGCCGGTGGTGATCAAGGACAATATCTGGGTTGGCGGC
TGGCGCATCACCCAAGGCTCCAAGCTGTTCGCGGGGTTCAAGGCGCCCGAGGATGCGATCGCGGTCGCGCGCCTGCGCCA
CGCCGGCGCGGTGATCGTGGGCATCGGCGCGACCTCGGAGTTCGCCTGCAAGGGCGTCACCACGTCGCTGCTGTATGGGC
CGACGCGGCATCCGCATAACCCGGATCTCACGCCCGGCGGATCGTCCGGCGGACCTGCCGTGGCGGTGGCCGCAGGTCTC
GTGCCGCTGGCGATCGGCACTGATGCGGGCGGCTCGAGCCGGCGGCCGCCCGCGCATGTTGGCGTGGTCGGCTTCAAGCC
GTCCTATGGCGCCGTTCCCTATGGACCCGGCTTTGCCGAGGCCGGATGGGGCATCTCCGTCGTGGCGCCCATCGCAGCAC
ATGTCGAGGATGCGAGGCTCGCTTTCGAAGCCATGGCCGGGATCGATCCGCGCGATCCGGCGACGTTCGATCTGCCAAAG
GGGGATGCATCACAGCCGCTGCGCATGGCGTTCTCGCCGCGCCTCGGGCTCGACGTCGCGGTCGACGACGAAGTCGGCAG
CGGTCTGCAGCGAGCGATTGATCGGCTGCGCGCGGGAGGAATGCCGATCGCAGATGCCGATCCCATCTGGCCGCCAGACC
TCGCCGAGGACGCGGTGATGCCGCTGCAGCATGCCGGGCTGGCCGCGCTGTTTGGTGACGCCTTTCGCCATGATGCCACG
CAATTCGATCCTGATATCGCCAAGCAGATCGAGCGTGGCCTCGGCCTCGGCGGCGCCGATGTCGCGCATGCGCTGCAGGC
AAGCGCACGGATCGCGCGCGCCTTCGCCGCGTTTTTTCTGGACGTCGATGTGCTGCTGGCGCCGACCGTGCCATGTGTGG
CCTGGCCGCTCACGCAGCTCGGTCCGACGATGATCGGCGGCAGGCCGGCGAGCCCGCGCGGCCATGCGGTGTTCACGCCG
TTCGCCAATCACGCGGGAGTACCCGCGATCTCGATTCCGTGCGGGAGAGACGCAAAGGGTCTGCCGTTCGGACTGCAATT
GATCGCAGCGCGTGGCCGCGATCGCCTGCTGCTCGATGTCGCTGCGCAAACCGAACGCGTGCTAGCCGATCGAGCTTGA

Upstream 100 bases:

>100_bases
CTGGTCGCGCTGCTCACTCCCCGTGTCGGGCTGGGCGGCAGCTTCGTCGCCCTTGGACTGATGGCGGCCGCCGGAGCGGC
CGTTCTCCTGATGGAGCGTT

Downstream 100 bases:

>100_bases
ACAGCCTCCAGCATTGCGGGTGCTCACCTCGCTGGGCTTGCGCGGAGAGTTGGGCGCACCGCCTCTCATGGCAGCCAGCG
TCGACCCAGATCACCAGACC

Product: putative glutamyl-tRNA(Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MMRADEIAGTVLARQRSAREMAERALTRIDAARALNAVVTIDPERTRAEADAVDARVAAGEALPLAGVPVVIKDNIWVGG
WRITQGSKLFAGFKAPEDAIAVARLRHAGAVIVGIGATSEFACKGVTTSLLYGPTRHPHNPDLTPGGSSGGPAVAVAAGL
VPLAIGTDAGGSSRRPPAHVGVVGFKPSYGAVPYGPGFAEAGWGISVVAPIAAHVEDARLAFEAMAGIDPRDPATFDLPK
GDASQPLRMAFSPRLGLDVAVDDEVGSGLQRAIDRLRAGGMPIADADPIWPPDLAEDAVMPLQHAGLAALFGDAFRHDAT
QFDPDIAKQIERGLGLGGADVAHALQASARIARAFAAFFLDVDVLLAPTVPCVAWPLTQLGPTMIGGRPASPRGHAVFTP
FANHAGVPAISIPCGRDAKGLPFGLQLIAARGRDRLLLDVAAQTERVLADRA

Sequences:

>Translated_452_residues
MMRADEIAGTVLARQRSAREMAERALTRIDAARALNAVVTIDPERTRAEADAVDARVAAGEALPLAGVPVVIKDNIWVGG
WRITQGSKLFAGFKAPEDAIAVARLRHAGAVIVGIGATSEFACKGVTTSLLYGPTRHPHNPDLTPGGSSGGPAVAVAAGL
VPLAIGTDAGGSSRRPPAHVGVVGFKPSYGAVPYGPGFAEAGWGISVVAPIAAHVEDARLAFEAMAGIDPRDPATFDLPK
GDASQPLRMAFSPRLGLDVAVDDEVGSGLQRAIDRLRAGGMPIADADPIWPPDLAEDAVMPLQHAGLAALFGDAFRHDAT
QFDPDIAKQIERGLGLGGADVAHALQASARIARAFAAFFLDVDVLLAPTVPCVAWPLTQLGPTMIGGRPASPRGHAVFTP
FANHAGVPAISIPCGRDAKGLPFGLQLIAARGRDRLLLDVAAQTERVLADRA
>Mature_452_residues
MMRADEIAGTVLARQRSAREMAERALTRIDAARALNAVVTIDPERTRAEADAVDARVAAGEALPLAGVPVVIKDNIWVGG
WRITQGSKLFAGFKAPEDAIAVARLRHAGAVIVGIGATSEFACKGVTTSLLYGPTRHPHNPDLTPGGSSGGPAVAVAAGL
VPLAIGTDAGGSSRRPPAHVGVVGFKPSYGAVPYGPGFAEAGWGISVVAPIAAHVEDARLAFEAMAGIDPRDPATFDLPK
GDASQPLRMAFSPRLGLDVAVDDEVGSGLQRAIDRLRAGGMPIADADPIWPPDLAEDAVMPLQHAGLAALFGDAFRHDAT
QFDPDIAKQIERGLGLGGADVAHALQASARIARAFAAFFLDVDVLLAPTVPCVAWPLTQLGPTMIGGRPASPRGHAVFTP
FANHAGVPAISIPCGRDAKGLPFGLQLIAARGRDRLLLDVAAQTERVLADRA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=28.714859437751, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=26.3581488933602, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17537465, Length=485, Percent_Identity=24.3298969072165, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17543272, Length=199, Percent_Identity=33.1658291457286, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=160, Percent_Identity=30.625, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=162, Percent_Identity=33.9506172839506, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=285, Percent_Identity=27.3684210526316, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=394, Percent_Identity=29.9492385786802, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323950, Length=227, Percent_Identity=30.8370044052863, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648113, Length=484, Percent_Identity=29.7520661157025, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45550774, Length=488, Percent_Identity=26.0245901639344, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648435, Length=488, Percent_Identity=26.0245901639344, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648437, Length=488, Percent_Identity=26.0245901639344, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161078093, Length=495, Percent_Identity=26.2626262626263, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24644968, Length=440, Percent_Identity=25.6818181818182, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21356731, Length=494, Percent_Identity=24.6963562753036, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648441, Length=433, Percent_Identity=26.0969976905312, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648439, Length=433, Percent_Identity=26.0969976905312, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24652985, Length=488, Percent_Identity=26.2295081967213, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922090, Length=488, Percent_Identity=26.2295081967213, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652981, Length=488, Percent_Identity=26.2295081967213, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652983, Length=488, Percent_Identity=26.2295081967213, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 46737; Mature: 46737

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00539 PYROKININ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRADEIAGTVLARQRSAREMAERALTRIDAARALNAVVTIDPERTRAEADAVDARVAAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHCC
EALPLAGVPVVIKDNIWVGGWRITQGSKLFAGFKAPEDAIAVARLRHAGAVIVGIGATSE
CCCCCCCCCEEEECCEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCC
FACKGVTTSLLYGPTRHPHNPDLTPGGSSGGPAVAVAAGLVPLAIGTDAGGSSRRPPAHV
HHHCCCHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEECCCCCCCCCCCCEE
GVVGFKPSYGAVPYGPGFAEAGWGISVVAPIAAHVEDARLAFEAMAGIDPRDPATFDLPK
EEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC
GDASQPLRMAFSPRLGLDVAVDDEVGSGLQRAIDRLRAGGMPIADADPIWPPDLAEDAVM
CCCCCCCEEEECCCCCCEEEECHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHC
PLQHAGLAALFGDAFRHDATQFDPDIAKQIERGLGLGGADVAHALQASARIARAFAAFFL
CHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
DVDVLLAPTVPCVAWPLTQLGPTMIGGRPASPRGHAVFTPFANHAGVPAISIPCGRDAKG
HHHHHHHCCCCHHHCCHHHCCCHHCCCCCCCCCCCEEECCCCCCCCCCEEECCCCCCCCC
LPFGLQLIAARGRDRLLLDVAAQTERVLADRA
CCHHHHHHHHCCCCEEEEEEHHHHHHHHHCCC
>Mature Secondary Structure
MMRADEIAGTVLARQRSAREMAERALTRIDAARALNAVVTIDPERTRAEADAVDARVAAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHCC
EALPLAGVPVVIKDNIWVGGWRITQGSKLFAGFKAPEDAIAVARLRHAGAVIVGIGATSE
CCCCCCCCCEEEECCEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCC
FACKGVTTSLLYGPTRHPHNPDLTPGGSSGGPAVAVAAGLVPLAIGTDAGGSSRRPPAHV
HHHCCCHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEECCCCCCCCCCCCEE
GVVGFKPSYGAVPYGPGFAEAGWGISVVAPIAAHVEDARLAFEAMAGIDPRDPATFDLPK
EEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC
GDASQPLRMAFSPRLGLDVAVDDEVGSGLQRAIDRLRAGGMPIADADPIWPPDLAEDAVM
CCCCCCCEEEECCCCCCEEEECHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHC
PLQHAGLAALFGDAFRHDATQFDPDIAKQIERGLGLGGADVAHALQASARIARAFAAFFL
CHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
DVDVLLAPTVPCVAWPLTQLGPTMIGGRPASPRGHAVFTPFANHAGVPAISIPCGRDAKG
HHHHHHHCCCCHHHCCHHHCCCHHCCCCCCCCCCCEEECCCCCCCCCCEEECCCCCCCCC
LPFGLQLIAARGRDRLLLDVAAQTERVLADRA
CCHHHHHHHHCCCCEEEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA