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The map label for this gene is ntrB [H]

Identifier: 146337899

GI number: 146337899

Start: 829469

End: 830944

Strand: Direct

Name: ntrB [H]

Synonym: BRADO0785

Alternate gene names: 146337899

Gene position: 829469-830944 (Clockwise)

Preceding gene: 146337888

Following gene: 146337901

Centisome position: 11.12

GC content: 67.68

Gene sequence:

>1476_bases
GTGACAGAGAGCGACAACCAGCCGATCCTGGAGCGTCTGCGACCGAAACGGTCGCGCCGGCTCGGCTTGTCCGGAAAGCT
GCTGTTGTTGACCATTCCGCTGGTGATGATCGCGGAAGTCCTGATCTACGTACCCGCGATCGCCAACTTCTGGAGCAATC
GACTGAACGATCGACTGGCGGCCGCCAATACCGCTGCTCTCGTGCTCGACGCAGCGCCCTCCGGCATGGTTCCGGACTCG
CTGGCGCGGCAGATCCTGACCTCGATCAACGCGCGCGGCGTCGCCATCAAGATGGGCCAGCAGCGGCGACTGCTGGCGAG
CGCCGATCTGCCCGCCTCGATCGACCACGACTTCGACATGCGCGACATGACCGTGTGGTCGTCGATCGCGAATTCCCTGA
AGATGCTGACCGAGACCGGCGATCATACGATGCGCGTGATTGGCCCGGCGCCGGGCCGCGCCCAGTTCATCGAGGTTGTC
ACCGACGAGAAGCCGCTGCGCAAGGCGATGTACCGCTTCTCGCGCAACCTGCTCGGCGTGTCGCTCCTGCTGGCGATGCT
GACCGCGGCCCTGGTGTATTTTGCCCTGCATTTCCTGTTCGTGCGGCCGATGCGGCGCCTGACAGCGAGCCTGGTCGGCT
TCCATGAGAATCCGGAGAGCGCGGCGCGCATCATCGTGCCGAGCCAGCGCTCCGACGAGATCGGCGTCGCCGAGCGCGAG
CTCGCCGACATGCAGCGCGATCTCGTCTCGATGCTGCATCAGAAGAGCCGGCTCGCCGCGCTCGGCCTTGCCGTCTCCAA
GATCAACCACGATCTGCGCAACCTGCTGGCCTCTTCGCAGCTGTTGTCGGACCAACTCGCCAGCGTGCCGGATCCGCGGG
TCCAGCGCTTCGCGCCGAAGCTGATGCGCTCGCTCGAGCGCGCCATCGCGTTCTGCCAATCCACGCTGTCTTACGGCAAG
GTGCAGGAGCCGGCGCCGGACCGCCGCATGGTCACCGTCGAGCCCGTGATCGCGGAGGTGCGGGAGACAGCCGGGCTGTC
GCCGGACGCCTCGATCACCTGGATCGCCGCGGTCGAGCGCGGCCTCAAGATCGATGCCGATCCGGATCAGCTGTTCCGCG
TGCTGCTCAACCTGGTCCGGAATGCTGCGCAGGCCCTGGACAGCCTCGCGGGCGACGCGGGCGTGCGCCAGATCCGCATC
ACCGGACGCCGTGAGGGCGCGGTGACCATCCTGGAAGTCTCCGATACCGGACCGGGCGTTCCGGCGAAGGTGCGCGAGCA
CCTGTTCGAAGCCTTCCGCGGGGCCGGCCGCCCCGGCGGTTCGGGCCTGGGCCTCGCCATCGCCGCGGAGCTGGTCCGCG
CCCATGACGGCGACATCCAGCTGGTCGAGGGCACGCTCGGGGCCACTTTCCGAATCACGATCCCCGACCGCCCGGTCGAA
CTGCAGAGCTTCTGGACCGAGCGCGCCAGCGCCTGA

Upstream 100 bases:

>100_bases
TGGAAACCTCAACATGAGCGATCCGTAATTTGACCGGACTCCGGTGCAGTCCCATCTTGCGGACGCGTAAGGGTGGGGGT
GTCTCTTACAAGGGCGATAC

Downstream 100 bases:

>100_bases
GCCGCCGCCGTTCGGCACCAAAAAATGCTCGCAAAATGTCCCGCTCCAGCCTTGCCAAGCGGCTTCCGAGCCGTTAGTCA
GAGGCCCTTCGCGAGGCCAG

Product: putative sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MTESDNQPILERLRPKRSRRLGLSGKLLLLTIPLVMIAEVLIYVPAIANFWSNRLNDRLAAANTAALVLDAAPSGMVPDS
LARQILTSINARGVAIKMGQQRRLLASADLPASIDHDFDMRDMTVWSSIANSLKMLTETGDHTMRVIGPAPGRAQFIEVV
TDEKPLRKAMYRFSRNLLGVSLLLAMLTAALVYFALHFLFVRPMRRLTASLVGFHENPESAARIIVPSQRSDEIGVAERE
LADMQRDLVSMLHQKSRLAALGLAVSKINHDLRNLLASSQLLSDQLASVPDPRVQRFAPKLMRSLERAIAFCQSTLSYGK
VQEPAPDRRMVTVEPVIAEVRETAGLSPDASITWIAAVERGLKIDADPDQLFRVLLNLVRNAAQALDSLAGDAGVRQIRI
TGRREGAVTILEVSDTGPGVPAKVREHLFEAFRGAGRPGGSGLGLAIAAELVRAHDGDIQLVEGTLGATFRITIPDRPVE
LQSFWTERASA

Sequences:

>Translated_491_residues
MTESDNQPILERLRPKRSRRLGLSGKLLLLTIPLVMIAEVLIYVPAIANFWSNRLNDRLAAANTAALVLDAAPSGMVPDS
LARQILTSINARGVAIKMGQQRRLLASADLPASIDHDFDMRDMTVWSSIANSLKMLTETGDHTMRVIGPAPGRAQFIEVV
TDEKPLRKAMYRFSRNLLGVSLLLAMLTAALVYFALHFLFVRPMRRLTASLVGFHENPESAARIIVPSQRSDEIGVAERE
LADMQRDLVSMLHQKSRLAALGLAVSKINHDLRNLLASSQLLSDQLASVPDPRVQRFAPKLMRSLERAIAFCQSTLSYGK
VQEPAPDRRMVTVEPVIAEVRETAGLSPDASITWIAAVERGLKIDADPDQLFRVLLNLVRNAAQALDSLAGDAGVRQIRI
TGRREGAVTILEVSDTGPGVPAKVREHLFEAFRGAGRPGGSGLGLAIAAELVRAHDGDIQLVEGTLGATFRITIPDRPVE
LQSFWTERASA
>Mature_490_residues
TESDNQPILERLRPKRSRRLGLSGKLLLLTIPLVMIAEVLIYVPAIANFWSNRLNDRLAAANTAALVLDAAPSGMVPDSL
ARQILTSINARGVAIKMGQQRRLLASADLPASIDHDFDMRDMTVWSSIANSLKMLTETGDHTMRVIGPAPGRAQFIEVVT
DEKPLRKAMYRFSRNLLGVSLLLAMLTAALVYFALHFLFVRPMRRLTASLVGFHENPESAARIIVPSQRSDEIGVAEREL
ADMQRDLVSMLHQKSRLAALGLAVSKINHDLRNLLASSQLLSDQLASVPDPRVQRFAPKLMRSLERAIAFCQSTLSYGKV
QEPAPDRRMVTVEPVIAEVRETAGLSPDASITWIAAVERGLKIDADPDQLFRVLLNLVRNAAQALDSLAGDAGVRQIRIT
GRREGAVTILEVSDTGPGVPAKVREHLFEAFRGAGRPGGSGLGLAIAAELVRAHDGDIQLVEGTLGATFRITIPDRPVEL
QSFWTERASA

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=328, Percent_Identity=27.4390243902439, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=27.2340425531915, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790436, Length=115, Percent_Identity=39.1304347826087, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786912, Length=234, Percent_Identity=29.0598290598291, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53664; Mature: 53533

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESDNQPILERLRPKRSRRLGLSGKLLLLTIPLVMIAEVLIYVPAIANFWSNRLNDRLA
CCCCCCCHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AANTAALVLDAAPSGMVPDSLARQILTSINARGVAIKMGQQRRLLASADLPASIDHDFDM
HCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCC
RDMTVWSSIANSLKMLTETGDHTMRVIGPAPGRAQFIEVVTDEKPLRKAMYRFSRNLLGV
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SLLLAMLTAALVYFALHFLFVRPMRRLTASLVGFHENPESAARIIVPSQRSDEIGVAERE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHH
LADMQRDLVSMLHQKSRLAALGLAVSKINHDLRNLLASSQLLSDQLASVPDPRVQRFAPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LMRSLERAIAFCQSTLSYGKVQEPAPDRRMVTVEPVIAEVRETAGLSPDASITWIAAVER
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEHHHHHC
GLKIDADPDQLFRVLLNLVRNAAQALDSLAGDAGVRQIRITGRREGAVTILEVSDTGPGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCC
PAKVREHLFEAFRGAGRPGGSGLGLAIAAELVRAHDGDIQLVEGTLGATFRITIPDRPVE
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCH
LQSFWTERASA
HHHHHHHHCCC
>Mature Secondary Structure 
TESDNQPILERLRPKRSRRLGLSGKLLLLTIPLVMIAEVLIYVPAIANFWSNRLNDRLA
CCCCCCHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AANTAALVLDAAPSGMVPDSLARQILTSINARGVAIKMGQQRRLLASADLPASIDHDFDM
HCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCC
RDMTVWSSIANSLKMLTETGDHTMRVIGPAPGRAQFIEVVTDEKPLRKAMYRFSRNLLGV
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SLLLAMLTAALVYFALHFLFVRPMRRLTASLVGFHENPESAARIIVPSQRSDEIGVAERE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHH
LADMQRDLVSMLHQKSRLAALGLAVSKINHDLRNLLASSQLLSDQLASVPDPRVQRFAPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LMRSLERAIAFCQSTLSYGKVQEPAPDRRMVTVEPVIAEVRETAGLSPDASITWIAAVER
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEHHHHHC
GLKIDADPDQLFRVLLNLVRNAAQALDSLAGDAGVRQIRITGRREGAVTILEVSDTGPGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCC
PAKVREHLFEAFRGAGRPGGSGLGLAIAAELVRAHDGDIQLVEGTLGATFRITIPDRPVE
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCH
LQSFWTERASA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]