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The map label for this gene is truA [H]

Identifier: 146337887

GI number: 146337887

Start: 816913

End: 817650

Strand: Direct

Name: truA [H]

Synonym: BRADO0772

Alternate gene names: 146337887

Gene position: 816913-817650 (Clockwise)

Preceding gene: 146337886

Following gene: 146337888

Centisome position: 10.96

GC content: 66.53

Gene sequence:

>738_bases
ATGCCCCGCTACAAGCTCACCATCGAATATGACGGCGCGCCGTTCTGCGGCTGGCAGCTGCAAGAGACGCTGCCGTCGGT
GCAGGGCGTGCTCGAGGCGGCCGTGCAGGCGACCTGCGGTGTGCCGACCCGCGTGCACGGCTCGGGTCGAACCGACGCCG
GCGTCCACGCCACCGGCCAAGTCGCGCATTGCGACATCGACAAGGAGTTCCGTCCTGACAAATTGCGCGACGCGCTCAAC
GCCCATCTGCGGCCGAACCCGGTTGCCGTGCTCGACGCGCAGATCGTACCCGACACGTTCGAAGCCCGCTTCTCGGCGCG
CAAGCGGCACTACCGCTATCGGATCGTCAACCGCCGTTCCAATCTCGCGCTCGAGGTCGGCCGCGTCTGGCGGGTGCCGC
AGCGGCTCGATTCGGATGCGATGCACGTCGCCGCGCAGCGGCTGATCGGCAAGCATGATTTCACGACGTTCCGCGACACA
GAATGTCAGGCGAAGTCGCCCGAGAAGACACTCGATCAGCTCGACGTCGTCAGGGATGGAGATGCTGTCACCATTATCAC
CTCGGCACGCTCTTATTTGCACAGCCAGGTGCGCTCGATGGTCGGCTCGCTGGTCTGGGTCGGGCAGGGCCGCTGGAGCG
CTGATGATCTGGCAGCCGCCCTGGCGGCGCGCCGCCGCGCGGCCTGCGGAGTGGTCGCTCCGCCGGAGGGACTCTATTTG
GTGCAGGTCGACTATTGA

Upstream 100 bases:

>100_bases
GCCGCGGGCTTGACCCGCGCATCCATCTCGCGAACAAGCTTCATGCTTGGATGGATTGCCGGGTCGAGCCCGGCAATGAC
GGCCAAACGCAATCGAGGCG

Downstream 100 bases:

>100_bases
GATCCGATCTTCAGTGGAACCGGTCGCCGACAACATTAACATATGCGAATGAGCATCCCGATGAGTTGCTATTCTGCATT
GCGGGCCAGACTTCCTTAAC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MPRYKLTIEYDGAPFCGWQLQETLPSVQGVLEAAVQATCGVPTRVHGSGRTDAGVHATGQVAHCDIDKEFRPDKLRDALN
AHLRPNPVAVLDAQIVPDTFEARFSARKRHYRYRIVNRRSNLALEVGRVWRVPQRLDSDAMHVAAQRLIGKHDFTTFRDT
ECQAKSPEKTLDQLDVVRDGDAVTIITSARSYLHSQVRSMVGSLVWVGQGRWSADDLAAALAARRRAACGVVAPPEGLYL
VQVDY

Sequences:

>Translated_245_residues
MPRYKLTIEYDGAPFCGWQLQETLPSVQGVLEAAVQATCGVPTRVHGSGRTDAGVHATGQVAHCDIDKEFRPDKLRDALN
AHLRPNPVAVLDAQIVPDTFEARFSARKRHYRYRIVNRRSNLALEVGRVWRVPQRLDSDAMHVAAQRLIGKHDFTTFRDT
ECQAKSPEKTLDQLDVVRDGDAVTIITSARSYLHSQVRSMVGSLVWVGQGRWSADDLAAALAARRRAACGVVAPPEGLYL
VQVDY
>Mature_244_residues
PRYKLTIEYDGAPFCGWQLQETLPSVQGVLEAAVQATCGVPTRVHGSGRTDAGVHATGQVAHCDIDKEFRPDKLRDALNA
HLRPNPVAVLDAQIVPDTFEARFSARKRHYRYRIVNRRSNLALEVGRVWRVPQRLDSDAMHVAAQRLIGKHDFTTFRDTE
CQAKSPEKTLDQLDVVRDGDAVTIITSARSYLHSQVRSMVGSLVWVGQGRWSADDLAAALAARRRAACGVVAPPEGLYLV
QVDY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=273, Percent_Identity=34.7985347985348, Blast_Score=112, Evalue=2e-25,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=42.2764227642276, Blast_Score=176, Evalue=2e-45,
Organism=Drosophila melanogaster, GI116007752, Length=303, Percent_Identity=29.042904290429, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27199; Mature: 27068

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYKLTIEYDGAPFCGWQLQETLPSVQGVLEAAVQATCGVPTRVHGSGRTDAGVHATGQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
VAHCDIDKEFRPDKLRDALNAHLRPNPVAVLDAQIVPDTFEARFSARKRHYRYRIVNRRS
EEEECCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHEEEECCCC
NLALEVGRVWRVPQRLDSDAMHVAAQRLIGKHDFTTFRDTECQAKSPEKTLDQLDVVRDG
CCEEEHHHHHCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHEECC
DAVTIITSARSYLHSQVRSMVGSLVWVGQGRWSADDLAAALAARRRAACGVVAPPEGLYL
CEEEEEHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
VQVDY
EEECC
>Mature Secondary Structure 
PRYKLTIEYDGAPFCGWQLQETLPSVQGVLEAAVQATCGVPTRVHGSGRTDAGVHATGQ
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
VAHCDIDKEFRPDKLRDALNAHLRPNPVAVLDAQIVPDTFEARFSARKRHYRYRIVNRRS
EEEECCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHEEEECCCC
NLALEVGRVWRVPQRLDSDAMHVAAQRLIGKHDFTTFRDTECQAKSPEKTLDQLDVVRDG
CCEEEHHHHHCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHEECC
DAVTIITSARSYLHSQVRSMVGSLVWVGQGRWSADDLAAALAARRRAACGVVAPPEGLYL
CEEEEEHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
VQVDY
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA