Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 146337885

GI number: 146337885

Start: 815192

End: 815719

Strand: Direct

Name: def [H]

Synonym: BRADO0770

Alternate gene names: 146337885

Gene position: 815192-815719 (Clockwise)

Preceding gene: 146337881

Following gene: 146337886

Centisome position: 10.93

GC content: 63.07

Gene sequence:

>528_bases
ATGTCCTTAAGAGAAATCATCATCCTGCCCGACCGGCAGCTCCGGCTGGTGTCCAAGCCTGTCGAGAAGGTCACGCCGGA
GATCCGGCAGCTGGTCGACGACATGTTCCAGACCATGTACGACGCACCCGGCATCGGGCTCGCGGCGATCCAGGTCGCGC
AGCCCCTGCGCGTCATCACCATGGACCTCGCCAAGCCGGATTCCGGCGGCGAGACCAAGCGCGAGCCGCGGGTCTTCATC
AATCCGGAGATCATCGCCAAGTCCGACGAGCTGTCGGTGTATGAGGAAGGCTGCCTGTCGATCCCCGAATATTACGAGGA
GGTCGAGCGGCCGGCGCGCGTGCGCGTGCGCTTCACCGATCTCGACGGCGTGGTTCGCGAGGAGGATGCCGAGGGTCTCT
ACGCCACCTGCATCCAGCATGAGATCGACCATCTCAACGGCGTGCTGTTCATCGACTATCTGTCGAAGCTCAAGCGCGAC
CGCGTGATGAAGAAGTTCACCAAGGCCGCCAAGCGCGCCGGCGAGTAA

Upstream 100 bases:

>100_bases
AGAAGCGTGGTTAACGGACCGTTGAAATTCATGGTTAGCGATGTCTTAACCGCGCGCCGTCACATTGACCGCGCCTGTTC
CGGGGCTTAAATCGCGCGCC

Downstream 100 bases:

>100_bases
CGACTAATCTCTCTCTTCCGTCATGCCCGGGCTTGTCCCGAGCATCCACGTCTCTCCATCCGGCACTGCCGCGAGACGTG
GATGGCCGGGACAAGCCCGG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSLREIIILPDRQLRLVSKPVEKVTPEIRQLVDDMFQTMYDAPGIGLAAIQVAQPLRVITMDLAKPDSGGETKREPRVFI
NPEIIAKSDELSVYEEGCLSIPEYYEEVERPARVRVRFTDLDGVVREEDAEGLYATCIQHEIDHLNGVLFIDYLSKLKRD
RVMKKFTKAAKRAGE

Sequences:

>Translated_175_residues
MSLREIIILPDRQLRLVSKPVEKVTPEIRQLVDDMFQTMYDAPGIGLAAIQVAQPLRVITMDLAKPDSGGETKREPRVFI
NPEIIAKSDELSVYEEGCLSIPEYYEEVERPARVRVRFTDLDGVVREEDAEGLYATCIQHEIDHLNGVLFIDYLSKLKRD
RVMKKFTKAAKRAGE
>Mature_174_residues
SLREIIILPDRQLRLVSKPVEKVTPEIRQLVDDMFQTMYDAPGIGLAAIQVAQPLRVITMDLAKPDSGGETKREPRVFIN
PEIIAKSDELSVYEEGCLSIPEYYEEVERPARVRVRFTDLDGVVREEDAEGLYATCIQHEIDHLNGVLFIDYLSKLKRDR
VMKKFTKAAKRAGE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=33.1210191082803, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=50, Blast_Score=152, Evalue=8e-39,
Organism=Drosophila melanogaster, GI24645728, Length=183, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24645726, Length=143, Percent_Identity=29.3706293706294, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19976; Mature: 19845

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLREIIILPDRQLRLVSKPVEKVTPEIRQLVDDMFQTMYDAPGIGLAAIQVAQPLRVIT
CCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHH
MDLAKPDSGGETKREPRVFINPEIIAKSDELSVYEEGCLSIPEYYEEVERPARVRVRFTD
HHCCCCCCCCCCCCCCEEEECCCEEECCCCHHHHHHHCCCCHHHHHHHCCCCEEEEEEEC
LDGVVREEDAEGLYATCIQHEIDHLNGVLFIDYLSKLKRDRVMKKFTKAAKRAGE
CCCCHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLREIIILPDRQLRLVSKPVEKVTPEIRQLVDDMFQTMYDAPGIGLAAIQVAQPLRVIT
CCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHH
MDLAKPDSGGETKREPRVFINPEIIAKSDELSVYEEGCLSIPEYYEEVERPARVRVRFTD
HHCCCCCCCCCCCCCCEEEECCCEEECCCCHHHHHHHCCCCHHHHHHHCCCCEEEEEEEC
LDGVVREEDAEGLYATCIQHEIDHLNGVLFIDYLSKLKRDRVMKKFTKAAKRAGE
CCCCHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA