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The map label for this gene is rlmH

Identifier: 146337572

GI number: 146337572

Start: 438752

End: 439240

Strand: Reverse

Name: rlmH

Synonym: BRADO0427

Alternate gene names: 146337572

Gene position: 439240-438752 (Counterclockwise)

Preceding gene: 146337573

Following gene: 146337571

Centisome position: 5.89

GC content: 66.46

Gene sequence:

>489_bases
GTGCACATGCGCCTCGTCGTGATCGCCATCGGCCGGTTGAAGCAGGGTCCGGAGCGGCAGCTCGCGGAGCGTTACCGCGA
GCGTTTCGACGACATTGGCCGCAAGCTCGGCTTTCGCGGCCTCGACATCCATGAAATTCCAGAAAGCCGCGCCCGCGATG
CGGCGACGCGCATGGCCGAGGAGGCCGCGGCGATCGCAGCCGCGATTCCCGACAACTCCATGCTCGTCTGCCTGGACGAG
CGGGGCCACAACATCGACAGCGCAACCTTTGCGGGTCATATCGGACGGTGGCGTGACGAGGGCGTCGCGGCGACGGCCTT
CGTGATCGGCGGTGCCGACGGACTTTCGCCCGAATTGCGGCGCAAAGCCAAGCTCGGGGTCGCGTTCGGCGCTGCGACCT
GGCCGCACCAGATCGTCCGTGTCCTGCTGCTCGAGCAGATTTACCGTACTGCTACGATCCTGGCCGGTCACCCCTATCAC
CGCGCTTGA

Upstream 100 bases:

>100_bases
GAGTTCTACAATCTCGAGCGAATGTGGACGCAAGGGCCAAAGCCGGCGAAGACGCTGTAGCCGCCGCGGGCTGCGAATCG
CGCGGTGCACGTGCTAGGGC

Downstream 100 bases:

>100_bases
ATCAGGGACGATCGGAGCACAATAAGGACGTCGATGGTTTTCGCGCGGCCCCTTTCTCTCTCCCCGATTCCGGCCCGCCG
CGGCTGGGCGCGTCGCGCGT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MHMRLVVIAIGRLKQGPERQLAERYRERFDDIGRKLGFRGLDIHEIPESRARDAATRMAEEAAAIAAAIPDNSMLVCLDE
RGHNIDSATFAGHIGRWRDEGVAATAFVIGGADGLSPELRRKAKLGVAFGAATWPHQIVRVLLLEQIYRTATILAGHPYH
RA

Sequences:

>Translated_162_residues
MHMRLVVIAIGRLKQGPERQLAERYRERFDDIGRKLGFRGLDIHEIPESRARDAATRMAEEAAAIAAAIPDNSMLVCLDE
RGHNIDSATFAGHIGRWRDEGVAATAFVIGGADGLSPELRRKAKLGVAFGAATWPHQIVRVLLLEQIYRTATILAGHPYH
RA
>Mature_162_residues
MHMRLVVIAIGRLKQGPERQLAERYRERFDDIGRKLGFRGLDIHEIPESRARDAATRMAEEAAAIAAAIPDNSMLVCLDE
RGHNIDSATFAGHIGRWRDEGVAATAFVIGGADGLSPELRRKAKLGVAFGAATWPHQIVRVLLLEQIYRTATILAGHPYH
RA

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=30.188679245283, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_BRASO (A4YKE8)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001202620.1
- ProteinModelPortal:   A4YKE8
- STRING:   A4YKE8
- GeneID:   5116225
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO0427
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   ILSGHPY
- ProtClustDB:   PRK00103
- BioCyc:   BSP376:BRADO0427-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17900; Mature: 17900

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMRLVVIAIGRLKQGPERQLAERYRERFDDIGRKLGFRGLDIHEIPESRARDAATRMAE
CCEEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHH
EAAAIAAAIPDNSMLVCLDERGHNIDSATFAGHIGRWRDEGVAATAFVIGGADGLSPELR
HHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHH
RKAKLGVAFGAATWPHQIVRVLLLEQIYRTATILAGHPYHRA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHMRLVVIAIGRLKQGPERQLAERYRERFDDIGRKLGFRGLDIHEIPESRARDAATRMAE
CCEEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHH
EAAAIAAAIPDNSMLVCLDERGHNIDSATFAGHIGRWRDEGVAATAFVIGGADGLSPELR
HHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHH
RKAKLGVAFGAATWPHQIVRVLLLEQIYRTATILAGHPYHRA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA