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The map label for this gene is hemN [H]

Identifier: 146337343

GI number: 146337343

Start: 187328

End: 188485

Strand: Direct

Name: hemN [H]

Synonym: BRADO0175

Alternate gene names: 146337343

Gene position: 187328-188485 (Clockwise)

Preceding gene: 146337342

Following gene: 146337345

Centisome position: 2.51

GC content: 67.62

Gene sequence:

>1158_bases
TTGAGCAGCGCTGAGAGCGAAGCGTTCGGAGTCTATGTGCACTGGCCGTTCTGCCTGTCGAAATGTCCGTATTGCGACTT
CAACAGCCACGTCCGCCACGCCGCCATCGACCAGGACCGTTTTGCTCGCGCCTTCGCGCAGGAGATCGCCAACACTGCGG
CGCGCACCGGGCCGCGCACCGTCAGCTCGATCTTTCTCGGCGGCGGCACGCCGTCGCTGATGCAGCCGCAGACCGTCGGC
GCCATTCTCGATGCCATCGGCAAACACTGGCACGTCGCCCCCGATGTCGAGGTGTCGCTCGAAGCCAACCCGACCAGCGT
CGAGGCCACGCGCTTTGCAGGCTATCGTGCGGCCGGCGTCAACCGTGTCTCACTCGGCGTCCAGGCGATGGACGATGCGT
CGCTGAAGATGCTGGGACGACTGCACACCGCAGAAGAAGCGATGAAAGCGGTGGCGATCGCGCGGGGCGCGTTCGAGCGC
TATTCCTTCGATCTGATCTATGCGCGGCCCGACCAGACGGCTGCGATGTGGACCGAGGAGCTGACGCGCGCGATCGGTGA
AGCCGCCGAGCATCTGTCGCTGTACCAGCTGACGATCGAGGAGGGCACGCCGTTCTTCGGGCTGCACGCCGCCGGCAAGC
TGAAGACGCTGGACGAGGGCCTGGCGCGCACGCTGTACGACGTCACGCAGGAGATCTGCGCCCGCCATGGCCTGCCGGCC
TATGAGATCTCCAACCATGCGCGTCCCGGCGCGGAGTGCCGGCACAATCTCGTCTACTGGCGCGGCCAGGAATATGCCGG
CATCGGTCCGGGCGCGCACGGGCGGCTCGATATCGAGGGCATCCGCCACGCCACCGCGACCGACAAGCGGCCGGAAGCCT
GGCTGATGCGGGTCGAGACAAACGGCCACGGCGTGATGACCGACGACCTGCTCAACAGCGAGGAGCGCGCCGACGAATTC
CTGCTGATGGGGCTACGCCTGAGCGAGGGCATCGATCCCCGACGCTATGCCGCGATCGCCGGACGTACGCTCGATCCGCG
CCGCATTGCGCTGTTGCGCGAGGAGGGCGCCATCGCCGTGGAGCCGGACGGCCGGCTGCGCGTCACCCAGGACGGCTTCC
CCGTGCTCGACGCCGTCGTCGCCGATCTCGCCGCGTGA

Upstream 100 bases:

>100_bases
TTCGGCGAGATGACCAGCATCGAGAAGCACGGGCTGCCGCCGCTCGGCCTCGGCCTGTCGCACCGGGCGCGCGCCTTCGT
GAAGCTCGCGGAGATCTGTC

Downstream 100 bases:

>100_bases
GCTGTGGAAAGGCGTGGCTCATCATGCCGCGCCGGTGTCGGAACCCCGACCCTCCTGATGAACACGCCTCTCGGCGTGTT
CATGGTCGGTGTGCTGTGCC

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSSAESEAFGVYVHWPFCLSKCPYCDFNSHVRHAAIDQDRFARAFAQEIANTAARTGPRTVSSIFLGGGTPSLMQPQTVG
AILDAIGKHWHVAPDVEVSLEANPTSVEATRFAGYRAAGVNRVSLGVQAMDDASLKMLGRLHTAEEAMKAVAIARGAFER
YSFDLIYARPDQTAAMWTEELTRAIGEAAEHLSLYQLTIEEGTPFFGLHAAGKLKTLDEGLARTLYDVTQEICARHGLPA
YEISNHARPGAECRHNLVYWRGQEYAGIGPGAHGRLDIEGIRHATATDKRPEAWLMRVETNGHGVMTDDLLNSEERADEF
LLMGLRLSEGIDPRRYAAIAGRTLDPRRIALLREEGAIAVEPDGRLRVTQDGFPVLDAVVADLAA

Sequences:

>Translated_385_residues
MSSAESEAFGVYVHWPFCLSKCPYCDFNSHVRHAAIDQDRFARAFAQEIANTAARTGPRTVSSIFLGGGTPSLMQPQTVG
AILDAIGKHWHVAPDVEVSLEANPTSVEATRFAGYRAAGVNRVSLGVQAMDDASLKMLGRLHTAEEAMKAVAIARGAFER
YSFDLIYARPDQTAAMWTEELTRAIGEAAEHLSLYQLTIEEGTPFFGLHAAGKLKTLDEGLARTLYDVTQEICARHGLPA
YEISNHARPGAECRHNLVYWRGQEYAGIGPGAHGRLDIEGIRHATATDKRPEAWLMRVETNGHGVMTDDLLNSEERADEF
LLMGLRLSEGIDPRRYAAIAGRTLDPRRIALLREEGAIAVEPDGRLRVTQDGFPVLDAVVADLAA
>Mature_384_residues
SSAESEAFGVYVHWPFCLSKCPYCDFNSHVRHAAIDQDRFARAFAQEIANTAARTGPRTVSSIFLGGGTPSLMQPQTVGA
ILDAIGKHWHVAPDVEVSLEANPTSVEATRFAGYRAAGVNRVSLGVQAMDDASLKMLGRLHTAEEAMKAVAIARGAFERY
SFDLIYARPDQTAAMWTEELTRAIGEAAEHLSLYQLTIEEGTPFFGLHAAGKLKTLDEGLARTLYDVTQEICARHGLPAY
EISNHARPGAECRHNLVYWRGQEYAGIGPGAHGRLDIEGIRHATATDKRPEAWLMRVETNGHGVMTDDLLNSEERADEFL
LMGLRLSEGIDPRRYAAIAGRTLDPRRIALLREEGAIAVEPDGRLRVTQDGFPVLDAVVADLAA

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=300, Percent_Identity=40, Blast_Score=232, Evalue=5e-61,
Organism=Escherichia coli, GI1789325, Length=345, Percent_Identity=35.0724637681159, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87082341, Length=248, Percent_Identity=28.2258064516129, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 42095; Mature: 41964

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAESEAFGVYVHWPFCLSKCPYCDFNSHVRHAAIDQDRFARAFAQEIANTAARTGPRT
CCCCCCCEEEEEEECHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCE
VSSIFLGGGTPSLMQPQTVGAILDAIGKHWHVAPDVEVSLEANPTSVEATRFAGYRAAGV
EEEEEECCCCCCCCCCHHHHHHHHHHCCCEECCCCCEEEEECCCCCCCHHHHCCCHHCCC
NRVSLGVQAMDDASLKMLGRLHTAEEAMKAVAIARGAFERYSFDLIYARPDQTAAMWTEE
CEEEECCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHH
LTRAIGEAAEHLSLYQLTIEEGTPFFGLHAAGKLKTLDEGLARTLYDVTQEICARHGLPA
HHHHHHHHHHHEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
YEISNHARPGAECRHNLVYWRGQEYAGIGPGAHGRLDIEGIRHATATDKRPEAWLMRVET
HHHCCCCCCCHHHHCCCEEEECCCCCCCCCCCCCEEEHHHCCCCCCCCCCCCEEEEEEEE
NGHGVMTDDLLNSEERADEFLLMGLRLSEGIDPRRYAAIAGRTLDPRRIALLREEGAIAV
CCCEEEEHHHCCCHHHHHHHEEEEEHHHCCCCHHHHHHHHCCCCCCHHEEEEECCCCEEE
EPDGRLRVTQDGFPVLDAVVADLAA
CCCCCEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SSAESEAFGVYVHWPFCLSKCPYCDFNSHVRHAAIDQDRFARAFAQEIANTAARTGPRT
CCCCCCEEEEEEECHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCE
VSSIFLGGGTPSLMQPQTVGAILDAIGKHWHVAPDVEVSLEANPTSVEATRFAGYRAAGV
EEEEEECCCCCCCCCCHHHHHHHHHHCCCEECCCCCEEEEECCCCCCCHHHHCCCHHCCC
NRVSLGVQAMDDASLKMLGRLHTAEEAMKAVAIARGAFERYSFDLIYARPDQTAAMWTEE
CEEEECCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHH
LTRAIGEAAEHLSLYQLTIEEGTPFFGLHAAGKLKTLDEGLARTLYDVTQEICARHGLPA
HHHHHHHHHHHEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
YEISNHARPGAECRHNLVYWRGQEYAGIGPGAHGRLDIEGIRHATATDKRPEAWLMRVET
HHHCCCCCCCHHHHCCCEEEECCCCCCCCCCCCCEEEHHHCCCCCCCCCCCCEEEEEEEE
NGHGVMTDDLLNSEERADEFLLMGLRLSEGIDPRRYAAIAGRTLDPRRIALLREEGAIAV
CCCEEEEHHHCCCHHHHHHHEEEEEHHHCCCCHHHHHHHHCCCCCCHHEEEEECCCCEEE
EPDGRLRVTQDGFPVLDAVVADLAA
CCCCCEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8757728; 8969508; 9384377; 1339421 [H]