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The map label for this gene is rph [H]

Identifier: 146337341

GI number: 146337341

Start: 185987

End: 186700

Strand: Direct

Name: rph [H]

Synonym: BRADO0173

Alternate gene names: 146337341

Gene position: 185987-186700 (Clockwise)

Preceding gene: 146337337

Following gene: 146337342

Centisome position: 2.49

GC content: 69.33

Gene sequence:

>714_bases
ATGCGGCCGAGCCGCCGTGCACCGGACGAAATGCGGGCCGTGAGCCTGGAACGAGGCGTCGTGAAATACGCCGAGGGCTC
CTGCCTGGTGAAGTTCGGCGACACCCATGTGCTGGTCACCGCGACCCTGGAGGACCGGCTGCCGCCCTGGCTCAAGGGGC
AGGGCCGCGGCTGGATCACCGCCGAATACGGCATGCTGCCGCGCGCCACCCTGGAGCGCACCCGCCGCGAGGCGTCAGCC
GGCAAGCAGACCGGCCGCACGGTCGAGATCCAGCGCCTGATCGGCCGCTCGCTGCGCACCGCAATCGACCTGGAAGCGCT
CGGCGAGCGCCAGATCACGGTCGATTGCGACGTGCTGCAGGCCGATGGCGGCACCCGCACCGCTTCGATCACCGGCGCCT
GGGTGGCGTTGGCCGACTGCATCCGCTGGATGAAGGCGCGCAACATGATCAAGACCGAGGTGCTGCGTACCAACGTCGCC
GCGGTCTCGTGCGGCATCTACAACGGCACGCCGGTGCTCGACCTCGACTATGCCGAGGATTCCGAGGCCGAGACCGACGC
CAATTTCGTCATGACCGGCGACGGCCGCATCATCGAGGTGCAGGGCACCGCCGAGAAGACCCCGTTCAGCGAGGCCGAGT
TCCTCGCGCTGATGGCGCTGGCGCGCAAGGGCGTCGGCCGGCTGGTCGACCTGCAGAAGATGGCGGTCGCGTGA

Upstream 100 bases:

>100_bases
CGCGCCCGGTTAAGCCCTTGATTAAGCCCTTGCCGCTGCCGGTCGCGGCTCCTACAAGGCCGGTCAAATCGGTCCATTGC
CTTTTTCACGGAGAGAACCC

Downstream 100 bases:

>100_bases
GCAGTTCCCACCGCCAGATCACCGGGCGCCTCGTGATTGCGACCCACAATGCGGGCAAGCTCGTCGAGATGCGCGAGCTG
CTGGCGCCCCACGGCGTCGA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRPSRRAPDEMRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEDRLPPWLKGQGRGWITAEYGMLPRATLERTRREASA
GKQTGRTVEIQRLIGRSLRTAIDLEALGERQITVDCDVLQADGGTRTASITGAWVALADCIRWMKARNMIKTEVLRTNVA
AVSCGIYNGTPVLDLDYAEDSEAETDANFVMTGDGRIIEVQGTAEKTPFSEAEFLALMALARKGVGRLVDLQKMAVA

Sequences:

>Translated_237_residues
MRPSRRAPDEMRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEDRLPPWLKGQGRGWITAEYGMLPRATLERTRREASA
GKQTGRTVEIQRLIGRSLRTAIDLEALGERQITVDCDVLQADGGTRTASITGAWVALADCIRWMKARNMIKTEVLRTNVA
AVSCGIYNGTPVLDLDYAEDSEAETDANFVMTGDGRIIEVQGTAEKTPFSEAEFLALMALARKGVGRLVDLQKMAVA
>Mature_237_residues
MRPSRRAPDEMRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEDRLPPWLKGQGRGWITAEYGMLPRATLERTRREASA
GKQTGRTVEIQRLIGRSLRTAIDLEALGERQITVDCDVLQADGGTRTASITGAWVALADCIRWMKARNMIKTEVLRTNVA
AVSCGIYNGTPVLDLDYAEDSEAETDANFVMTGDGRIIEVQGTAEKTPFSEAEFLALMALARKGVGRLVDLQKMAVA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=63.5071090047393, Blast_Score=267, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI71981632, Length=177, Percent_Identity=27.683615819209, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25930; Mature: 25930

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSRRAPDEMRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEDRLPPWLKGQGRGWIT
CCCCCCCCHHHHHHHHHHCEEEECCCCEEEEECCCEEEEEEEHHHCCCCCCCCCCCEEEE
AEYGMLPRATLERTRREASAGKQTGRTVEIQRLIGRSLRTAIDLEALGERQITVDCDVLQ
ECCCCCCHHHHHHHHHHHHCCHHCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGAWVALADCIRWMKARNMIKTEVLRTNVAAVSCGIYNGTPVLDLDYAED
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCC
SEAETDANFVMTGDGRIIEVQGTAEKTPFSEAEFLALMALARKGVGRLVDLQKMAVA
CCCCCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MRPSRRAPDEMRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLEDRLPPWLKGQGRGWIT
CCCCCCCCHHHHHHHHHHCEEEECCCCEEEEECCCEEEEEEEHHHCCCCCCCCCCCEEEE
AEYGMLPRATLERTRREASAGKQTGRTVEIQRLIGRSLRTAIDLEALGERQITVDCDVLQ
ECCCCCCHHHHHHHHHHHHCCHHCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGAWVALADCIRWMKARNMIKTEVLRTNVAAVSCGIYNGTPVLDLDYAED
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCC
SEAETDANFVMTGDGRIIEVQGTAEKTPFSEAEFLALMALARKGVGRLVDLQKMAVA
CCCCCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA