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The map label for this gene is pheT [H]

Identifier: 146337310

GI number: 146337310

Start: 152212

End: 154620

Strand: Direct

Name: pheT [H]

Synonym: BRADO0141

Alternate gene names: 146337310

Gene position: 152212-154620 (Clockwise)

Preceding gene: 146337309

Following gene: 146337311

Centisome position: 2.04

GC content: 67.16

Gene sequence:

>2409_bases
ATGAAATTCACACTGTCCTGGCTGAAGGACCATCTCGACACCGATGAGCCGGTGGAGAAACTCGCCGACAAGCTCACCAT
GATCGGCCTTGAGGTCGAGCATCTCGAGGACAAGGCGAAGCTGCTCGCGCCCTATAAAATCGCGCGCGTCGTCTCCGCCG
AGCAGCATCCCAACGCCGATCGCCTGCGCGTCTGCATGGTCGATACCGGTGACGGTGGCGCGCCGGTGCAGGTCGTGTGC
GGCGCGCCGAATGCGCGGGCGGGGCTGGTGTCGGTGTTCTCGCCGCCGGGCACCTACATTCCCGGCAAGAACATCACACT
GTCGGTCGGCACGATCAGAGGCGTCGAGAGCCGCGGCATGCTGTGCTCGGAGGCCGAGCTCGAATTGTCGGAGAACCACG
ACGGCATCATGGAGCTGCCGGCCGATGCGCCGATCGGCAAGCCCTATGCGGAGTGGGCCGGGCTCGGCGATCCCCTGTTC
GAGATCAATCTGACGCCGAATCGCCAGGACTGCACGGGCGTACACGGCATCGCGCGCGATCTCGCCGCCGCCGACATGGG
GAAGCTGAAAGACCCCGGCATCAAGCCGATCAAGGGCGAATTCCCCTGCCCCGTGAAGGTCGCGGTCGAGGACGAGAAGC
TGTGCCCCGGCTTCGCACTGCGGCTGGTGCGCGGCGTCAAGAACGGCCCGTCGCCGAAATGGCTGCAGCAGCGCCTGGCC
TCGATCGGGCTGCGCCCGATCAACGCACTGGTCGACGTCACCAACTACATGACCTTCGATCGCGCGCGGCCGCTGCACGT
GTTCGACGCCAGGAAGGTCAAGGGCAATCTGGTCGTCCGCCGCGCCCGCGACGGCGAGACGCTGCTGGCGCTCGACGGCC
GCACCTACACGCTCGACCATTCGATGTGTGTCATCGCCGATGACGCCGGCGTCGAATCGCTCGCCGGCATCATGGGCGGC
GAAGCCTCGGGCTGCGACGAGGGCACCACCGACGTGCTGATCGAATCGGCGCTGTGGAACGAGATCAACATCGCCCAGAC
CGGCCGCAAGCTCGGCATCAATTCCGACGCGCGCTACCGCTTCGAGCGCGGCGTCGATCCGGCCTTCATGGTGCCCGGCC
TGGAGATGGCGACCAAGCTCGTGATCGATCTCTGCGGCGGCTCGCCGTCGGAGAACGTCGTCGTCGGCAACGCCTATGGC
GACGACCGCATCATCGACTTCCCCGTCGCCGAGGTGAAGCGCCTCGCCGGCATCGAGGTGCCGTTCGCAGAGATGCGGCA
CATCCTGACCCATCTCGGCTTCACGCTGGCCGGCAGCGGTCCGGTGGTGAAGGTCGCGGTGCCCTCGTGGCGCAGCGACG
TGCACGGCAAGGCCGATCTCGTCGAGGAGATCGTCCGCATGGTCGGCGTCGACAAGGTGCCGATGACGCCGTTCGAACGT
GGCGACGCGCCGCGCAAGCCGGTGCTGACGCAGCTGCAGCTCCGCACCCGCCGCGCCAAGCGCGCGCTGGCCGCGCGCGG
CATGGTCGAGGCCGTGACGTGGTCGTTCATATCCAAGCCTGCCGCCAAGCTGTTCGGCGGCGGCAAGCCGGAGCTTGCGC
TCGCAAATCCAATCGCGTCCGATCTCTCCGACATGCGCCCCAGCCTCTTGCCCGGCCTGATCGCGACCGTGCAGGCCAAT
GCCGATCGCGGCTTCGGGGATGCCGCGATCTTCGAGGTCGGACAGACCTTCAGGGGTGATCGTCCGGAGGATCAGTTCGT
CGCCGCCAGCGGCATCCGCCGCGGTATCGCCTCGTCGAAGGGTCACGGCCGGCATTGGACCGGCTCGCAGCCGGCGAACG
CGTATGACGCGAAGGCCGACGCATTCGCGGTGCTCGCCGCGGCGGGTGCGCCGATGAGCTCATTGCAGATCGTCGCCGGG
GGACCTGCCTGGATGCATCCGGGCCGCTCCGGCACGATCCAGATGGGACCGCAGAACGTGCTCGGGCATTTCGGCGAGCT
GCATCCGCGCACGCTCGAAGCGCTGAAGGCCGATGGTCCCCTGGTCGCGTTCGAGGTCATCCTCGATCGCATTCCCGCGG
CCAAGGCGAAGCCGACACGCGCCAAGCCGGTGCTGGAGCTCTCGGCCTTCCAGCCGGTCTCGCGCGACTTTGCCTTCATC
GTCGACCGCAAAGTGAAGGCCGGCGATATCGTGAAGGCGGCGCAGGGCGTCGACAAGAAGCTGATCTCCGACGTGACCGT
GTTCGACGTCTATGAGGGCAAGGGCATCGAGGACGGCAAGAAATCGGTCGCCATCGCCGTCACCCTTCAGCCGCGCGACA
AGACCATGACCGACGAGGAGATCGACGCCGTCGCGGCGAAGGTCACCGCCGACGTGGCCAAGAAGACCGGCGGCACGTTG
CGCGGATGA

Upstream 100 bases:

>100_bases
GAACCTATTTCACCCGACTGGGCAAGTTCAGCGACACCATGATGTTGATGTGTGAGCGCTTTCGTCTTGTCGAGGTCTTT
GCTGATCGCAGGATTGCATC

Downstream 100 bases:

>100_bases
GCCTGCAAGAGCTCATCCGAACCGAGCTCATCCCTGCCGATCTCAGCCTGTTCGCGACGGTTGCACTCGCCATCGTCGCT
CTCATCGCAGCGACCGCTCG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 802; Mature: 802

Protein sequence:

>802_residues
MKFTLSWLKDHLDTDEPVEKLADKLTMIGLEVEHLEDKAKLLAPYKIARVVSAEQHPNADRLRVCMVDTGDGGAPVQVVC
GAPNARAGLVSVFSPPGTYIPGKNITLSVGTIRGVESRGMLCSEAELELSENHDGIMELPADAPIGKPYAEWAGLGDPLF
EINLTPNRQDCTGVHGIARDLAAADMGKLKDPGIKPIKGEFPCPVKVAVEDEKLCPGFALRLVRGVKNGPSPKWLQQRLA
SIGLRPINALVDVTNYMTFDRARPLHVFDARKVKGNLVVRRARDGETLLALDGRTYTLDHSMCVIADDAGVESLAGIMGG
EASGCDEGTTDVLIESALWNEINIAQTGRKLGINSDARYRFERGVDPAFMVPGLEMATKLVIDLCGGSPSENVVVGNAYG
DDRIIDFPVAEVKRLAGIEVPFAEMRHILTHLGFTLAGSGPVVKVAVPSWRSDVHGKADLVEEIVRMVGVDKVPMTPFER
GDAPRKPVLTQLQLRTRRAKRALAARGMVEAVTWSFISKPAAKLFGGGKPELALANPIASDLSDMRPSLLPGLIATVQAN
ADRGFGDAAIFEVGQTFRGDRPEDQFVAASGIRRGIASSKGHGRHWTGSQPANAYDAKADAFAVLAAAGAPMSSLQIVAG
GPAWMHPGRSGTIQMGPQNVLGHFGELHPRTLEALKADGPLVAFEVILDRIPAAKAKPTRAKPVLELSAFQPVSRDFAFI
VDRKVKAGDIVKAAQGVDKKLISDVTVFDVYEGKGIEDGKKSVAIAVTLQPRDKTMTDEEIDAVAAKVTADVAKKTGGTL
RG

Sequences:

>Translated_802_residues
MKFTLSWLKDHLDTDEPVEKLADKLTMIGLEVEHLEDKAKLLAPYKIARVVSAEQHPNADRLRVCMVDTGDGGAPVQVVC
GAPNARAGLVSVFSPPGTYIPGKNITLSVGTIRGVESRGMLCSEAELELSENHDGIMELPADAPIGKPYAEWAGLGDPLF
EINLTPNRQDCTGVHGIARDLAAADMGKLKDPGIKPIKGEFPCPVKVAVEDEKLCPGFALRLVRGVKNGPSPKWLQQRLA
SIGLRPINALVDVTNYMTFDRARPLHVFDARKVKGNLVVRRARDGETLLALDGRTYTLDHSMCVIADDAGVESLAGIMGG
EASGCDEGTTDVLIESALWNEINIAQTGRKLGINSDARYRFERGVDPAFMVPGLEMATKLVIDLCGGSPSENVVVGNAYG
DDRIIDFPVAEVKRLAGIEVPFAEMRHILTHLGFTLAGSGPVVKVAVPSWRSDVHGKADLVEEIVRMVGVDKVPMTPFER
GDAPRKPVLTQLQLRTRRAKRALAARGMVEAVTWSFISKPAAKLFGGGKPELALANPIASDLSDMRPSLLPGLIATVQAN
ADRGFGDAAIFEVGQTFRGDRPEDQFVAASGIRRGIASSKGHGRHWTGSQPANAYDAKADAFAVLAAAGAPMSSLQIVAG
GPAWMHPGRSGTIQMGPQNVLGHFGELHPRTLEALKADGPLVAFEVILDRIPAAKAKPTRAKPVLELSAFQPVSRDFAFI
VDRKVKAGDIVKAAQGVDKKLISDVTVFDVYEGKGIEDGKKSVAIAVTLQPRDKTMTDEEIDAVAAKVTADVAKKTGGTL
RG
>Mature_802_residues
MKFTLSWLKDHLDTDEPVEKLADKLTMIGLEVEHLEDKAKLLAPYKIARVVSAEQHPNADRLRVCMVDTGDGGAPVQVVC
GAPNARAGLVSVFSPPGTYIPGKNITLSVGTIRGVESRGMLCSEAELELSENHDGIMELPADAPIGKPYAEWAGLGDPLF
EINLTPNRQDCTGVHGIARDLAAADMGKLKDPGIKPIKGEFPCPVKVAVEDEKLCPGFALRLVRGVKNGPSPKWLQQRLA
SIGLRPINALVDVTNYMTFDRARPLHVFDARKVKGNLVVRRARDGETLLALDGRTYTLDHSMCVIADDAGVESLAGIMGG
EASGCDEGTTDVLIESALWNEINIAQTGRKLGINSDARYRFERGVDPAFMVPGLEMATKLVIDLCGGSPSENVVVGNAYG
DDRIIDFPVAEVKRLAGIEVPFAEMRHILTHLGFTLAGSGPVVKVAVPSWRSDVHGKADLVEEIVRMVGVDKVPMTPFER
GDAPRKPVLTQLQLRTRRAKRALAARGMVEAVTWSFISKPAAKLFGGGKPELALANPIASDLSDMRPSLLPGLIATVQAN
ADRGFGDAAIFEVGQTFRGDRPEDQFVAASGIRRGIASSKGHGRHWTGSQPANAYDAKADAFAVLAAAGAPMSSLQIVAG
GPAWMHPGRSGTIQMGPQNVLGHFGELHPRTLEALKADGPLVAFEVILDRIPAAKAKPTRAKPVLELSAFQPVSRDFAFI
VDRKVKAGDIVKAAQGVDKKLISDVTVFDVYEGKGIEDGKKSVAIAVTLQPRDKTMTDEEIDAVAAKVTADVAKKTGGTL
RG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=815, Percent_Identity=34.8466257668712, Blast_Score=456, Evalue=1e-129,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 85735; Mature: 85735

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTLSWLKDHLDTDEPVEKLADKLTMIGLEVEHLEDKAKLLAPYKIARVVSAEQHPNAD
CCEEHHHHHHHCCCCHHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RLRVCMVDTGDGGAPVQVVCGAPNARAGLVSVFSPPGTYIPGKNITLSVGTIRGVESRGM
CEEEEEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEECEEECCCCCCC
LCSEAELELSENHDGIMELPADAPIGKPYAEWAGLGDPLFEINLTPNRQDCTGVHGIARD
EECCCCCEECCCCCCEEECCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHH
LAAADMGKLKDPGIKPIKGEFPCPVKVAVEDEKLCPGFALRLVRGVKNGPSPKWLQQRLA
HHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
SIGLRPINALVDVTNYMTFDRARPLHVFDARKVKGNLVVRRARDGETLLALDGRTYTLDH
HCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCEEEEECCEEEEECC
SMCVIADDAGVESLAGIMGGEASGCDEGTTDVLIESALWNEINIAQTGRKLGINSDARYR
CEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCCCCCCCHH
FERGVDPAFMVPGLEMATKLVIDLCGGSPSENVVVGNAYGDDRIIDFPVAEVKRLAGIEV
HHCCCCCEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEECCHHHHHHHHCCCC
PFAEMRHILTHLGFTLAGSGPVVKVAVPSWRSDVHGKADLVEEIVRMVGVDKVPMTPFER
CHHHHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
GDAPRKPVLTQLQLRTRRAKRALAARGMVEAVTWSFISKPAAKLFGGGKPELALANPIAS
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHCCCCCCEEECCHHHH
DLSDMRPSLLPGLIATVQANADRGFGDAAIFEVGQTFRGDRPEDQFVAASGIRRGIASSK
HHHHCCHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
GHGRHWTGSQPANAYDAKADAFAVLAAAGAPMSSLQIVAGGPAWMHPGRSGTIQMGPQNV
CCCCCCCCCCCCCCCCCCCHHHEEEECCCCCHHCEEEEECCCCEECCCCCCEEEECHHHH
LGHFGELHPRTLEALKADGPLVAFEVILDRIPAAKAKPTRAKPVLELSAFQPVSRDFAFI
HHHHHCCCHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCEEEE
VDRKVKAGDIVKAAQGVDKKLISDVTVFDVYEGKGIEDGKKSVAIAVTLQPRDKTMTDEE
EECCCCCCHHHHHHHCHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCHHH
IDAVAAKVTADVAKKTGGTLRG
HHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFTLSWLKDHLDTDEPVEKLADKLTMIGLEVEHLEDKAKLLAPYKIARVVSAEQHPNAD
CCEEHHHHHHHCCCCHHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RLRVCMVDTGDGGAPVQVVCGAPNARAGLVSVFSPPGTYIPGKNITLSVGTIRGVESRGM
CEEEEEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEECEEECCCCCCC
LCSEAELELSENHDGIMELPADAPIGKPYAEWAGLGDPLFEINLTPNRQDCTGVHGIARD
EECCCCCEECCCCCCEEECCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHH
LAAADMGKLKDPGIKPIKGEFPCPVKVAVEDEKLCPGFALRLVRGVKNGPSPKWLQQRLA
HHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
SIGLRPINALVDVTNYMTFDRARPLHVFDARKVKGNLVVRRARDGETLLALDGRTYTLDH
HCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCEEEEECCEEEEECC
SMCVIADDAGVESLAGIMGGEASGCDEGTTDVLIESALWNEINIAQTGRKLGINSDARYR
CEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCCCCCCCHH
FERGVDPAFMVPGLEMATKLVIDLCGGSPSENVVVGNAYGDDRIIDFPVAEVKRLAGIEV
HHCCCCCEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEECCHHHHHHHHCCCC
PFAEMRHILTHLGFTLAGSGPVVKVAVPSWRSDVHGKADLVEEIVRMVGVDKVPMTPFER
CHHHHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
GDAPRKPVLTQLQLRTRRAKRALAARGMVEAVTWSFISKPAAKLFGGGKPELALANPIAS
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHCCCCCCEEECCHHHH
DLSDMRPSLLPGLIATVQANADRGFGDAAIFEVGQTFRGDRPEDQFVAASGIRRGIASSK
HHHHCCHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
GHGRHWTGSQPANAYDAKADAFAVLAAAGAPMSSLQIVAGGPAWMHPGRSGTIQMGPQNV
CCCCCCCCCCCCCCCCCCCHHHEEEECCCCCHHCEEEEECCCCEECCCCCCEEEECHHHH
LGHFGELHPRTLEALKADGPLVAFEVILDRIPAAKAKPTRAKPVLELSAFQPVSRDFAFI
HHHHHCCCHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCEEEE
VDRKVKAGDIVKAAQGVDKKLISDVTVFDVYEGKGIEDGKKSVAIAVTLQPRDKTMTDEE
EECCCCCCHHHHHHHCHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCHHH
IDAVAAKVTADVAKKTGGTLRG
HHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]