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The map label for this gene is pheS [H]

Identifier: 146337308

GI number: 146337308

Start: 150720

End: 151802

Strand: Direct

Name: pheS [H]

Synonym: BRADO0139

Alternate gene names: 146337308

Gene position: 150720-151802 (Clockwise)

Preceding gene: 146337307

Following gene: 146337309

Centisome position: 2.02

GC content: 64.91

Gene sequence:

>1083_bases
GTGTCCGACCTCGCCTCGCTCGAAACCTCGATCCTCGACCAGATCGCCGCCGCTGGCGACGAAGCCGCGCTGGAAGCGGT
GCGCGTCGCGGCCCTCGGCAAGAAGGGCTCGATCTCGGCGTTGCTCGCCACGCTCGGCAAGATGTCGCCGGACGAGCGCA
AGACGCAAGGTGCTGCGATCAACCTCGCCAAGGACAAGGTCACGCAGGCGTTGTCCGCGCGCCGCGACGTGCTCAAGGCC
GCCGCGCTCGATGCCCGCCTCGCCGCCGAGACCATCGACGTCACCCTCCCGCTGCAGGATACGCCAGCCGATACGGGGCG
CATCCATCCGCTCAGCCAGGTGATGGACGAGCTGACCACGATCTTCGCCGACATGGGCTTCTCGATCGCCGAAGGTCCCG
ACGTCGAGACCGACGACTACAACTTCACCAAGCTGAATTTCCCGGAAGGCCATCCGGCGCGCGAGATGCACGACACGTTC
TTCTTCAATCCGAAGGAGGACGGCTCGCGCATGCTGCTGCGGACCCACACCTCGCCGGTGCAGGTACGCACGATGCTGAG
CCAGAAGCCGCCGATCCGCGTGATCTGCCCGGGCCGCACCTACCGCATCGATTCGGATGCGACCCACACGCCGCAATTCC
ACCAGGTCGAAGGCCTCGTCATCGACAAGGGCTCGCATCTCGGCCACCTCAAATGGATCCTGCACGAGTTCTGCAAGGCG
TTCTTCGAGGTCGACCACATCAACATGAAATTCCGGCCGTCGTTCTTCCCGTTCACCGAGCCGTCGCTCGAGGTCGACAT
CCAGTGCCGCCGCGACAAGGGCGAGATCCGGTTCGGCGAGGGCGAGGACTGGCTTGAGATTCTCGGCTGCGGCATGGTGC
ATCCGAACGTGCTGCGCGCCTGCGGCATCGATCCCGACGAGTACCAGGGCTTTGCCTGGGGCATGGGCATCGACCGCATC
GCGATGCTGAAATACGGCATGAGCGATCTGCGCCAGCTGTTCGAAGGCGACGTTCGCTGGCTGTCGCATTACGGCTTCAA
GCCGCTGGAGGTGCCGACCTTGGCGGGAGGACTGAGCACGTGA

Upstream 100 bases:

>100_bases
CGGCCGCTTTTTGCTGTCTAGCGCGATCACTCGTTTCGCTTGACCCCCGTCGCCGTCGCGGCGACAACCCAGCCGCCTTT
GCCCCGCGGAGATCTTGCCC

Downstream 100 bases:

>100_bases
GCGTCGAGATAGTTCCTGCAGCCTACCGCGGCTTGCGGTCGTTCGCGTTCGGCGACGGTCCTGAGCTTGCCGACAAATTG
CTCGACCTCGTCATCAAGGG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSDLASLETSILDQIAAAGDEAALEAVRVAALGKKGSISALLATLGKMSPDERKTQGAAINLAKDKVTQALSARRDVLKA
AALDARLAAETIDVTLPLQDTPADTGRIHPLSQVMDELTTIFADMGFSIAEGPDVETDDYNFTKLNFPEGHPAREMHDTF
FFNPKEDGSRMLLRTHTSPVQVRTMLSQKPPIRVICPGRTYRIDSDATHTPQFHQVEGLVIDKGSHLGHLKWILHEFCKA
FFEVDHINMKFRPSFFPFTEPSLEVDIQCRRDKGEIRFGEGEDWLEILGCGMVHPNVLRACGIDPDEYQGFAWGMGIDRI
AMLKYGMSDLRQLFEGDVRWLSHYGFKPLEVPTLAGGLST

Sequences:

>Translated_360_residues
MSDLASLETSILDQIAAAGDEAALEAVRVAALGKKGSISALLATLGKMSPDERKTQGAAINLAKDKVTQALSARRDVLKA
AALDARLAAETIDVTLPLQDTPADTGRIHPLSQVMDELTTIFADMGFSIAEGPDVETDDYNFTKLNFPEGHPAREMHDTF
FFNPKEDGSRMLLRTHTSPVQVRTMLSQKPPIRVICPGRTYRIDSDATHTPQFHQVEGLVIDKGSHLGHLKWILHEFCKA
FFEVDHINMKFRPSFFPFTEPSLEVDIQCRRDKGEIRFGEGEDWLEILGCGMVHPNVLRACGIDPDEYQGFAWGMGIDRI
AMLKYGMSDLRQLFEGDVRWLSHYGFKPLEVPTLAGGLST
>Mature_359_residues
SDLASLETSILDQIAAAGDEAALEAVRVAALGKKGSISALLATLGKMSPDERKTQGAAINLAKDKVTQALSARRDVLKAA
ALDARLAAETIDVTLPLQDTPADTGRIHPLSQVMDELTTIFADMGFSIAEGPDVETDDYNFTKLNFPEGHPAREMHDTFF
FNPKEDGSRMLLRTHTSPVQVRTMLSQKPPIRVICPGRTYRIDSDATHTPQFHQVEGLVIDKGSHLGHLKWILHEFCKAF
FEVDHINMKFRPSFFPFTEPSLEVDIQCRRDKGEIRFGEGEDWLEILGCGMVHPNVLRACGIDPDEYQGFAWGMGIDRIA
MLKYGMSDLRQLFEGDVRWLSHYGFKPLEVPTLAGGLST

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=274, Percent_Identity=33.2116788321168, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1788007, Length=344, Percent_Identity=52.0348837209302, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32563657, Length=271, Percent_Identity=32.4723247232472, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17508957, Length=271, Percent_Identity=32.4723247232472, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32566635, Length=264, Percent_Identity=31.0606060606061, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321087, Length=277, Percent_Identity=30.3249097472924, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=108, Percent_Identity=34.2592592592593, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI18858079, Length=275, Percent_Identity=32, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17137424, Length=299, Percent_Identity=25.4180602006689, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39914; Mature: 39783

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLASLETSILDQIAAAGDEAALEAVRVAALGKKGSISALLATLGKMSPDERKTQGAAI
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHCCCCEE
NLAKDKVTQALSARRDVLKAAALDARLAAETIDVTLPLQDTPADTGRIHPLSQVMDELTT
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
IFADMGFSIAEGPDVETDDYNFTKLNFPEGHPAREMHDTFFFNPKEDGSRMLLRTHTSPV
HHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCH
QVRTMLSQKPPIRVICPGRTYRIDSDATHTPQFHQVEGLVIDKGSHLGHLKWILHEFCKA
HHHHHHCCCCCEEEECCCCEEEECCCCCCCCCCEEECEEEEECCCCCHHHHHHHHHHHHH
FFEVDHINMKFRPSFFPFTEPSLEVDIQCRRDKGEIRFGEGEDWLEILGCGMVHPNVLRA
HHHHHCCCEEECCCCCCCCCCCEEEEEEEECCCCCEEECCCCHHHHHHHCCCCCHHHHHH
CGIDPDEYQGFAWGMGIDRIAMLKYGMSDLRQLFEGDVRWLSHYGFKPLEVPTLAGGLST
CCCCHHHCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDLASLETSILDQIAAAGDEAALEAVRVAALGKKGSISALLATLGKMSPDERKTQGAAI
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHCCCCEE
NLAKDKVTQALSARRDVLKAAALDARLAAETIDVTLPLQDTPADTGRIHPLSQVMDELTT
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
IFADMGFSIAEGPDVETDDYNFTKLNFPEGHPAREMHDTFFFNPKEDGSRMLLRTHTSPV
HHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCH
QVRTMLSQKPPIRVICPGRTYRIDSDATHTPQFHQVEGLVIDKGSHLGHLKWILHEFCKA
HHHHHHCCCCCEEEECCCCEEEECCCCCCCCCCEEECEEEEECCCCCHHHHHHHHHHHHH
FFEVDHINMKFRPSFFPFTEPSLEVDIQCRRDKGEIRFGEGEDWLEILGCGMVHPNVLRA
HHHHHCCCEEECCCCCCCCCCCEEEEEEEECCCCCEEECCCCHHHHHHHCCCCCHHHHHH
CGIDPDEYQGFAWGMGIDRIAMLKYGMSDLRQLFEGDVRWLSHYGFKPLEVPTLAGGLST
CCCCHHHCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]