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The map label for this gene is nusA [H]

Identifier: 146337225

GI number: 146337225

Start: 57489

End: 59096

Strand: Direct

Name: nusA [H]

Synonym: BRADO0051

Alternate gene names: 146337225

Gene position: 57489-59096 (Clockwise)

Preceding gene: 146337224

Following gene: 146337226

Centisome position: 0.77

GC content: 67.54

Gene sequence:

>1608_bases
ATGGCCGTCAGCGCCAACCGGTTGGAACTGCTGCAGATCGCCGACGCCGTCGCGCGCGAGAAGTCGATCGACCGCGGCAT
CGTGATCGCCGCCATGGAAGACGCGATCGCCAAGGCCGCCCGCGCCCGCTATGGCAGCGAGACCGACGTCCATGCCGAGA
TCGACCCGCGCAAGGGCGAGCTCCGGCTGTCGCGCCACATGCTGGTGGTCGAGAAGGTCGAGAACCCCTCCAACCAGATC
TCGCTCGATGACGCCCGCCGCGCCAATCCCGGCGCGCAGATCGGCGACACCATCGCCGATACGCTGCCGCCGCTGGAATA
TGGCCGCATCGCCGCGCAATCGGCCAAGCAGGTCATCGTGCAGAAGGTGCGCGAGGCCGAGCGCGACCGGCAGTACCAGG
AATTCAAGGACCGCATCGGCGACATCGTCAACGGCGTCGTCAAGCGCGTCGAATATGGCAGCGTGATCCTCGACCTCGGC
CGCGGCGAGGCGATCATCCGCCGCGACGAGATGCTGCCGCGCGAGGTGTTCCGCAACGGCGACCGCGTCCGCGCCTACGT
GTTCGACGTGCGCCGCGAGACCCGCGGCCCGCAGATCTTCCTGTCGCGCACCCATCCCCAGTTCATGGCCAAGCTGTTCG
CGCAGGAAGTGCCGGAGATCTACGACGGCATCGTTGAGATCAAGGCGGTGGCGCGCGATCCCGGCTCGCGTGCCAAGATC
GGCGTCGTGTCCCGCGACTCCTCGGTCGATCCGGTGGGCGCCTGCGTCGGCATGCGCGGCTCGCGCGTGCAGGCCGTGGT
GAACGAGCTGCAGGGCGAGAAGATCGACATCATCCCGTGGTCGCCCGATATCGCGACCTTCGTGGTCAACGCGCTGGCGC
CGGCCGAAGTCGCCAAGGTCGTCATCGACGAGGACCGCGAGCGCATCGAGGTCGTCGTCCCCGACACCAACAACCAGCTC
TCGCTGGCGATCGGACGCCGCGGCCAGAACGTCCGCCTCGCCTCGCAGCTGACCGGCTGGGACATCGACATCCTGACCGA
ACAGGAGGAATCGGAGCGCCGCCAGGCCGACTTCGAGAACTCGACCCGCGTATTCATGGAATCGCTCAACGTCGACGAAG
TCGTCGGCCAGCTCTTGGCGTCCGAAGGCTTCACCTCGGTGGAGGAGCTCGCGCTGGTCGATCTCAAGGATCTCGCCAGC
ATCGAGGGCTTCGACGAGGAGACCGCGACCGAGCTGCAGACCCGCGCCCGCGAATTCCTGGAGCAGCAGGAGGCCGAGCT
CGAGGCCAAGCGCAAGGAGCTCGGAGTCGAGGATGCGCTGAAGGACGTACCGGGCGTCACCTCCAAGATGCTGGTCAAGT
TCGGCGAGAACGACATCAAGACCGTCGACGACCTCGCCGGCTGCGCCACCGACGATCTGGTCGGCTGGGTCGAGCGCAAG
GACGGCGGCGAGCAGACCAAGTTCGCCGGCATTCTCGACGGGCTCGACGTCTCGCGCGACGACGCCGAGGCCATGGTCAT
GCAGGCCCGCGTCAAGGCCGGCTGGATCACCGAGGCCGATCTCGCCAAGGGCGAGGCGGAGGACGAAGCCGAAGGACAGC
CCGCCTGA

Upstream 100 bases:

>100_bases
CGGCCAAGAAGCCGCTGCCGACCGACACCAAGAAGCATCGCCTCGCCGCCGATCGTGCGCGCAGAGGCGAGATCGATCCT
GATTAGAGGAGATTTGAGCC

Downstream 100 bases:

>100_bases
CCCATGCTGGACGCCGCGATGACCCCCGCCATGACGTTGCTGGCTTGAGATGAGGAGCCGCACCACCGCATGCTCGCTTT
GGCCGACCCCGAGCTCGACA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MAVSANRLELLQIADAVAREKSIDRGIVIAAMEDAIAKAARARYGSETDVHAEIDPRKGELRLSRHMLVVEKVENPSNQI
SLDDARRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIVQKVREAERDRQYQEFKDRIGDIVNGVVKRVEYGSVILDLG
RGEAIIRRDEMLPREVFRNGDRVRAYVFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKI
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVAKVVIDEDRERIEVVVPDTNNQL
SLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQADFENSTRVFMESLNVDEVVGQLLASEGFTSVEELALVDLKDLAS
IEGFDEETATELQTRAREFLEQQEAELEAKRKELGVEDALKDVPGVTSKMLVKFGENDIKTVDDLAGCATDDLVGWVERK
DGGEQTKFAGILDGLDVSRDDAEAMVMQARVKAGWITEADLAKGEAEDEAEGQPA

Sequences:

>Translated_535_residues
MAVSANRLELLQIADAVAREKSIDRGIVIAAMEDAIAKAARARYGSETDVHAEIDPRKGELRLSRHMLVVEKVENPSNQI
SLDDARRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIVQKVREAERDRQYQEFKDRIGDIVNGVVKRVEYGSVILDLG
RGEAIIRRDEMLPREVFRNGDRVRAYVFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKI
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVAKVVIDEDRERIEVVVPDTNNQL
SLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQADFENSTRVFMESLNVDEVVGQLLASEGFTSVEELALVDLKDLAS
IEGFDEETATELQTRAREFLEQQEAELEAKRKELGVEDALKDVPGVTSKMLVKFGENDIKTVDDLAGCATDDLVGWVERK
DGGEQTKFAGILDGLDVSRDDAEAMVMQARVKAGWITEADLAKGEAEDEAEGQPA
>Mature_534_residues
AVSANRLELLQIADAVAREKSIDRGIVIAAMEDAIAKAARARYGSETDVHAEIDPRKGELRLSRHMLVVEKVENPSNQIS
LDDARRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIVQKVREAERDRQYQEFKDRIGDIVNGVVKRVEYGSVILDLGR
GEAIIRRDEMLPREVFRNGDRVRAYVFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKIG
VVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVAKVVIDEDRERIEVVVPDTNNQLS
LAIGRRGQNVRLASQLTGWDIDILTEQEESERRQADFENSTRVFMESLNVDEVVGQLLASEGFTSVEELALVDLKDLASI
EGFDEETATELQTRAREFLEQQEAELEAKRKELGVEDALKDVPGVTSKMLVKFGENDIKTVDDLAGCATDDLVGWVERKD
GGEQTKFAGILDGLDVSRDDAEAMVMQARVKAGWITEADLAKGEAEDEAEGQPA

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=466, Percent_Identity=44.4206008583691, Blast_Score=388, Evalue=1e-109,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 59071; Mature: 58940

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSANRLELLQIADAVAREKSIDRGIVIAAMEDAIAKAARARYGSETDVHAEIDPRKGE
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCC
LRLSRHMLVVEKVENPSNQISLDDARRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIV
HHHHHHHEEEEECCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QKVREAERDRQYQEFKDRIGDIVNGVVKRVEYGSVILDLGRGEAIIRRDEMLPREVFRNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEHHHHHCCHHHHHCCC
DRVRAYVFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKI
CEEEEHHEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVAKV
EEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCHHHHHHH
VIDEDRERIEVVVPDTNNQLSLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQADFEN
HHCCCCCEEEEEECCCCCEEEEEECCCCCCEEEHHHCCCCCEEEECCCHHHHHHHCCCCC
STRVFMESLNVDEVVGQLLASEGFTSVEELALVDLKDLASIEGFDEETATELQTRAREFL
HHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EQQEAELEAKRKELGVEDALKDVPGVTSKMLVKFGENDIKTVDDLAGCATDDLVGWVERK
HHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCC
DGGEQTKFAGILDGLDVSRDDAEAMVMQARVKAGWITEADLAKGEAEDEAEGQPA
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AVSANRLELLQIADAVAREKSIDRGIVIAAMEDAIAKAARARYGSETDVHAEIDPRKGE
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCC
LRLSRHMLVVEKVENPSNQISLDDARRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIV
HHHHHHHEEEEECCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QKVREAERDRQYQEFKDRIGDIVNGVVKRVEYGSVILDLGRGEAIIRRDEMLPREVFRNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEHHHHHCCHHHHHCCC
DRVRAYVFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKI
CEEEEHHEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVAKV
EEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCHHHHHHH
VIDEDRERIEVVVPDTNNQLSLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQADFEN
HHCCCCCEEEEEECCCCCEEEEEECCCCCCEEEHHHCCCCCEEEECCCHHHHHHHCCCCC
STRVFMESLNVDEVVGQLLASEGFTSVEELALVDLKDLASIEGFDEETATELQTRAREFL
HHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EQQEAELEAKRKELGVEDALKDVPGVTSKMLVKFGENDIKTVDDLAGCATDDLVGWVERK
HHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCC
DGGEQTKFAGILDGLDVSRDDAEAMVMQARVKAGWITEADLAKGEAEDEAEGQPA
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]