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The map label for this gene is mdlA [H]

Identifier: 146337197

GI number: 146337197

Start: 27293

End: 28381

Strand: Direct

Name: mdlA [H]

Synonym: BRADO0023

Alternate gene names: 146337197

Gene position: 27293-28381 (Clockwise)

Preceding gene: 146337193

Following gene: 146337200

Centisome position: 0.37

GC content: 68.23

Gene sequence:

>1089_bases
ATGGCACAGATCGCCGCGATCGAGACCGGCCTCTACCGCATTCCGCTCAGCGTCACGCTGACCGACAGCACGCATGGCGA
GATGACGGCGTTCGAGCTGATCACCTGCCGCGTGCGCGATGCCGATGGCGCCGAGGGCACAGGCTACACCTACACGGTGG
GCCGCAACGGCTATGCGGTGGCCGACATCTGCAGGCGCGAGATCCCTGATCTCGTCGCGGGTCACGAGGCCGACGACACC
GAGGCGATCTGGCACCGCGTGTGGTGGGGCCTGCATTATGGCGGCCGCGGCGGACCGGTGGTGCTGGCACTCTCGGCGCT
CGACATCGCGCTGTGGGACCTGAAGGCGCGGCGGGCCGGCCTGCCGCTGTTCCGCCTGCTGGGCGGCTATGACAGAAGCG
TGCCGTGCTATGCCGGCGGCATCGACCTCGATCTCTCTGTGGACGCGCTGCTGAAGCAAACCGACGACAATCTCGCCAGG
GGCTTCCGCGCGATCAAGATGAAAGTGGGACGGCCCGACCTTGGCGGCGACGTCGCGCGCGTCCGGGCGATGCGCCAACA
TCTCGGCGACGGCTTTCCGCTGATGGCCGATGCCAACATGAAATGGACCATCGAGGAGGCGATCCGCGCCGCGCGCGCCC
TGCAGCCGTTCGACCTCACCTGGCTGGAGGAGCCAACCATTCCCGACGACGTTGCCGGCCATGCGCGCATCCTGGCCGCC
GGTGGCGTGCCAATCGCGGCCGGCGAAAACCTGCGCTCGCTGTGGGATTTCAAGACCTACATCGCCGCCGGCGCCGTCTC
CTATCCCGAGCCCGACGTGACCAATTGCGGCGGCGTGACATCGTTCATGAAGATCGCGCGGCTGGCCGAGGCGTTCAACC
TGCCGGTCACGAGCCACGGCGCGCATGACGTCACCGTGCATCTGCTGGCGGCGTGCCCCAACCGCTCGTTCCTCGAAGCC
CACGGCTTCGGCCTCGACCGCTACATCGAGCATCCGCTGGTGCTGGAGAACGGCATGGCGCTCGCGCCGGCGCGGCCGGG
CCATGGCATGACCTTCGACTGGAAGGGACTTGCGACGCTCGCCGGGTGA

Upstream 100 bases:

>100_bases
TCGATCTCCAAGCCCTCTCGTCCGCCATGCGCCGTCCGCAATTGCCCTCGCAACAGCAGCAGCATAACCTTCGCCAAAAA
TCCAGGGAAGGAACTGCGCC

Downstream 100 bases:

>100_bases
GCCGAAGCGGAGCGCGCGGCGCAGCAGGTCAGGCCTTCGCACTCGGACGGCGCGACACGTCGTCGTACCATCGCCCGAGA
TGACGGAGATGATCCGGCGG

Product: putative mandelate racemase/muconate lactonizing family protein

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MAQIAAIETGLYRIPLSVTLTDSTHGEMTAFELITCRVRDADGAEGTGYTYTVGRNGYAVADICRREIPDLVAGHEADDT
EAIWHRVWWGLHYGGRGGPVVLALSALDIALWDLKARRAGLPLFRLLGGYDRSVPCYAGGIDLDLSVDALLKQTDDNLAR
GFRAIKMKVGRPDLGGDVARVRAMRQHLGDGFPLMADANMKWTIEEAIRAARALQPFDLTWLEEPTIPDDVAGHARILAA
GGVPIAAGENLRSLWDFKTYIAAGAVSYPEPDVTNCGGVTSFMKIARLAEAFNLPVTSHGAHDVTVHLLAACPNRSFLEA
HGFGLDRYIEHPLVLENGMALAPARPGHGMTFDWKGLATLAG

Sequences:

>Translated_362_residues
MAQIAAIETGLYRIPLSVTLTDSTHGEMTAFELITCRVRDADGAEGTGYTYTVGRNGYAVADICRREIPDLVAGHEADDT
EAIWHRVWWGLHYGGRGGPVVLALSALDIALWDLKARRAGLPLFRLLGGYDRSVPCYAGGIDLDLSVDALLKQTDDNLAR
GFRAIKMKVGRPDLGGDVARVRAMRQHLGDGFPLMADANMKWTIEEAIRAARALQPFDLTWLEEPTIPDDVAGHARILAA
GGVPIAAGENLRSLWDFKTYIAAGAVSYPEPDVTNCGGVTSFMKIARLAEAFNLPVTSHGAHDVTVHLLAACPNRSFLEA
HGFGLDRYIEHPLVLENGMALAPARPGHGMTFDWKGLATLAG
>Mature_361_residues
AQIAAIETGLYRIPLSVTLTDSTHGEMTAFELITCRVRDADGAEGTGYTYTVGRNGYAVADICRREIPDLVAGHEADDTE
AIWHRVWWGLHYGGRGGPVVLALSALDIALWDLKARRAGLPLFRLLGGYDRSVPCYAGGIDLDLSVDALLKQTDDNLARG
FRAIKMKVGRPDLGGDVARVRAMRQHLGDGFPLMADANMKWTIEEAIRAARALQPFDLTWLEEPTIPDDVAGHARILAAG
GVPIAAGENLRSLWDFKTYIAAGAVSYPEPDVTNCGGVTSFMKIARLAEAFNLPVTSHGAHDVTVHLLAACPNRSFLEAH
GFGLDRYIEHPLVLENGMALAPARPGHGMTFDWKGLATLAG

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=380, Percent_Identity=25.7894736842105, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI186972148, Length=314, Percent_Identity=27.3885350318471, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI42544117, Length=377, Percent_Identity=24.6684350132626, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1787864, Length=370, Percent_Identity=26.7567567567568, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI226510960, Length=283, Percent_Identity=29.6819787985866, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI48994953, Length=295, Percent_Identity=26.4406779661017, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 39109; Mature: 38978

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQIAAIETGLYRIPLSVTLTDSTHGEMTAFELITCRVRDADGAEGTGYTYTVGRNGYAV
CCCEEEECCCEEEEEEEEEEECCCCCCEEEEEEEEEEEECCCCCCCCCEEEEECCCCCHH
ADICRREIPDLVAGHEADDTEAIWHRVWWGLHYGGRGGPVVLALSALDIALWDLKARRAG
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCCCEEEEEEHHHHHHHHHHHHHCC
LPLFRLLGGYDRSVPCYAGGIDLDLSVDALLKQTDDNLARGFRAIKMKVGRPDLGGDVAR
CHHHHHHCCCCCCCCEEECCEEECCCHHHHHHHCCHHHHHHHEEEEEECCCCCCCCHHHH
VRAMRQHLGDGFPLMADANMKWTIEEAIRAARALQPFDLTWLEEPTIPDDVAGHARILAA
HHHHHHHHCCCCCEEECCCCEEEHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCEEEEEE
GGVPIAAGENLRSLWDFKTYIAAGAVSYPEPDVTNCGGVTSFMKIARLAEAFNLPVTSHG
CCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
AHDVTVHLLAACPNRSFLEAHGFGLDRYIEHPLVLENGMALAPARPGHGMTFDWKGLATL
CCCEEEEEEEECCCCCCHHHCCCCHHHHHCCCEEEECCCEECCCCCCCCCEEECCCHHCC
AG
CC
>Mature Secondary Structure 
AQIAAIETGLYRIPLSVTLTDSTHGEMTAFELITCRVRDADGAEGTGYTYTVGRNGYAV
CCEEEECCCEEEEEEEEEEECCCCCCEEEEEEEEEEEECCCCCCCCCEEEEECCCCCHH
ADICRREIPDLVAGHEADDTEAIWHRVWWGLHYGGRGGPVVLALSALDIALWDLKARRAG
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCCCEEEEEEHHHHHHHHHHHHHCC
LPLFRLLGGYDRSVPCYAGGIDLDLSVDALLKQTDDNLARGFRAIKMKVGRPDLGGDVAR
CHHHHHHCCCCCCCCEEECCEEECCCHHHHHHHCCHHHHHHHEEEEEECCCCCCCCHHHH
VRAMRQHLGDGFPLMADANMKWTIEEAIRAARALQPFDLTWLEEPTIPDDVAGHARILAA
HHHHHHHHCCCCCEEECCCCEEEHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCEEEEEE
GGVPIAAGENLRSLWDFKTYIAAGAVSYPEPDVTNCGGVTSFMKIARLAEAFNLPVTSHG
CCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
AHDVTVHLLAACPNRSFLEAHGFGLDRYIEHPLVLENGMALAPARPGHGMTFDWKGLATL
CCCEEEEEEEECCCCCCHHHCCCCHHHHHCCCEEEECCCEECCCCCCCCCEEECCCHHCC
AG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]