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The map label for this gene is rhlE [H]

Identifier: 146302773

GI number: 146302773

Start: 6084769

End: 6085434

Strand: Reverse

Name: rhlE [H]

Synonym: Fjoh_5046

Alternate gene names: 146302773

Gene position: 6085434-6084769 (Counterclockwise)

Preceding gene: 146302774

Following gene: 146302772

Centisome position: 99.81

GC content: 33.93

Gene sequence:

>666_bases
ATGAAACTAAAAAAGATAAACGAGAAATTACAAGACGGATTAATTGAAAATGGTTTAACAGAAGCAAATGTTTTGCAGAT
GGAAACTTTTTCGATCATAAAAAGCGGTGCAGATTGTATAATTATTTCTCCTGAAAAAAGCGGAAAAACCACTACAATTG
TCCTGAATGTTATTCAGCAGTTAGCTGGAAAAAATGAAGAATCGCCTCGTGCTTTGATTATTGTTGAAGACAAAGAAAAG
GTGCTTGAAATGGAAGAACTTTTTGAGAAATACGGAAAATATACCAATCTCGAAGTTTACGGTGTGCATGATAAAGGCGA
TATGGATTACGATAAAAATTATGTTTCTACCGGAATCGATGTTTTAATTGGAACGCCAAATAAATTAAGCGAAATGTTTT
CAACGGCTGGTTATAACGTAAATCGTTTAAGAATGTTTATCTTAGATGATGCTGATCCTATTTTAAAACTGCGTCATGAA
ACGAAGATCATGCGTATTTCAAACAGTATTGCTAAAACACAGCGTTTAATTTTTGCTGAAACATTGTCAGAGCGTATCGA
AATATTGGCAGATAAAATGCTAGTAGAGCCATATTTATTTGATATGGATGAAGAAGGCGAGGAGGAACTTGACGAAGAAG
ACGACGATATTGAGGAAGAAGAATAA

Upstream 100 bases:

>100_bases
GCTTCGCGCTTTAGGCTATAAGCTTTGGGCTTTAAGCTTTTATAGAAACTAAATAATTTTTGCTTACAGCGTTCAGCTTA
AAGCTTAAAGCATAAAAAAA

Downstream 100 bases:

>100_bases
TATGTAGAGAAACTTGAAATTGTAATAAACATCGAATGAGAAAACATCTTTTATATTTTTTGCTTACAATGTTTATTACA
ACTAGTTTTTTAAGTTGTGC

Product: DEAD/DEAH box helicase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKLKKINEKLQDGLIENGLTEANVLQMETFSIIKSGADCIIISPEKSGKTTTIVLNVIQQLAGKNEESPRALIIVEDKEK
VLEMEELFEKYGKYTNLEVYGVHDKGDMDYDKNYVSTGIDVLIGTPNKLSEMFSTAGYNVNRLRMFILDDADPILKLRHE
TKIMRISNSIAKTQRLIFAETLSERIEILADKMLVEPYLFDMDEEGEEELDEEDDDIEEEE

Sequences:

>Translated_221_residues
MKLKKINEKLQDGLIENGLTEANVLQMETFSIIKSGADCIIISPEKSGKTTTIVLNVIQQLAGKNEESPRALIIVEDKEK
VLEMEELFEKYGKYTNLEVYGVHDKGDMDYDKNYVSTGIDVLIGTPNKLSEMFSTAGYNVNRLRMFILDDADPILKLRHE
TKIMRISNSIAKTQRLIFAETLSERIEILADKMLVEPYLFDMDEEGEEELDEEDDDIEEEE
>Mature_221_residues
MKLKKINEKLQDGLIENGLTEANVLQMETFSIIKSGADCIIISPEKSGKTTTIVLNVIQQLAGKNEESPRALIIVEDKEK
VLEMEELFEKYGKYTNLEVYGVHDKGDMDYDKNYVSTGIDVLIGTPNKLSEMFSTAGYNVNRLRMFILDDADPILKLRHE
TKIMRISNSIAKTQRLIFAETLSERIEILADKMLVEPYLFDMDEEGEEELDEEDDDIEEEE

Specific function: Acts as an ATP-dependent RNA helicase, able to unwind ds-RNA [H]

COG id: COG0513

COG function: function code LKJ; Superfamily II DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI17505907, Length=212, Percent_Identity=27.8301886792453, Blast_Score=81, Evalue=6e-16,
Organism=Homo sapiens, GI20336298, Length=212, Percent_Identity=27.8301886792453, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI41327773, Length=200, Percent_Identity=25.5, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45446747, Length=210, Percent_Identity=23.3333333333333, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI45446743, Length=210, Percent_Identity=23.3333333333333, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI148613856, Length=195, Percent_Identity=26.1538461538462, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI38201710, Length=195, Percent_Identity=26.1538461538462, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI21071032, Length=225, Percent_Identity=24.4444444444444, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4758138, Length=191, Percent_Identity=24.0837696335079, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI13129006, Length=195, Percent_Identity=24.1025641025641, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI7661920, Length=193, Percent_Identity=23.8341968911917, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI222352149, Length=188, Percent_Identity=24.468085106383, Blast_Score=64, Evalue=8e-11,
Organism=Escherichia coli, GI1787016, Length=191, Percent_Identity=26.7015706806283, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507945, Length=214, Percent_Identity=28.5046728971963, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71987143, Length=195, Percent_Identity=21.5384615384615, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71999646, Length=198, Percent_Identity=22.2222222222222, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71999644, Length=198, Percent_Identity=22.2222222222222, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17509811, Length=199, Percent_Identity=25.6281407035176, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533224, Length=185, Percent_Identity=24.8648648648649, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17552642, Length=183, Percent_Identity=25.1366120218579, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17507879, Length=208, Percent_Identity=23.0769230769231, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17537549, Length=189, Percent_Identity=26.984126984127, Blast_Score=63, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322427, Length=183, Percent_Identity=26.775956284153, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320041, Length=194, Percent_Identity=23.7113402061856, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24662330, Length=218, Percent_Identity=25.6880733944954, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18859659, Length=221, Percent_Identity=27.1493212669683, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571338, Length=221, Percent_Identity=27.1493212669683, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17977678, Length=222, Percent_Identity=24.3243243243243, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281365720, Length=185, Percent_Identity=23.2432432432432, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24642151, Length=188, Percent_Identity=23.4042553191489, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI28574962, Length=187, Percent_Identity=22.9946524064171, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI18857967, Length=187, Percent_Identity=25.1336898395722, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR000629
- InterPro:   IPR014014 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: =3.6.4.13 [H]

Molecular weight: Translated: 25264; Mature: 25264

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKINEKLQDGLIENGLTEANVLQMETFSIIKSGADCIIISPEKSGKTTTIVLNVIQQ
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
LAGKNEESPRALIIVEDKEKVLEMEELFEKYGKYTNLEVYGVHDKGDMDYDKNYVSTGID
HHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCE
VLIGTPNKLSEMFSTAGYNVNRLRMFILDDADPILKLRHETKIMRISNSIAKTQRLIFAE
EEEECCHHHHHHHHHCCCCHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLSERIEILADKMLVEPYLFDMDEEGEEELDEEDDDIEEEE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKLKKINEKLQDGLIENGLTEANVLQMETFSIIKSGADCIIISPEKSGKTTTIVLNVIQQ
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
LAGKNEESPRALIIVEDKEKVLEMEELFEKYGKYTNLEVYGVHDKGDMDYDKNYVSTGID
HHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCE
VLIGTPNKLSEMFSTAGYNVNRLRMFILDDADPILKLRHETKIMRISNSIAKTQRLIFAE
EEEECCHHHHHHHHHCCCCHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLSERIEILADKMLVEPYLFDMDEEGEEELDEEDDDIEEEE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8037924; 8905232; 9278503; 1931833; 10361280 [H]