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The map label for this gene is cobB [H]

Identifier: 146302740

GI number: 146302740

Start: 6050678

End: 6051379

Strand: Direct

Name: cobB [H]

Synonym: Fjoh_5013

Alternate gene names: 146302740

Gene position: 6050678-6051379 (Clockwise)

Preceding gene: 146302735

Following gene: 146302741

Centisome position: 99.24

GC content: 38.32

Gene sequence:

>702_bases
ATGAAAAAGAAACTTGTGGTTTTAACCGGAGCCGGAATTAGTGCCGAAAGCGGTATTAAAACATTCCGCGACAGCGATGG
TTTATGGGAAGGACATGACGTTATGGAAGTAGCAACTCCAGAAGGATGGCAAAAAAATCAGGATCTGGTTTTGGACTTTT
ACAATAAAAGACGTCAGCAGTTAAAAGAAGTACAGCCCAACTTAGGCCACCAGATTCTGGCTGAATTAGAAAAAGATTTT
GATGTTTATATTATCACCCAAAATGTCGACGATTTGCACGAACGTGCCGGAAGCACAAAAGTACTGCACTTACATGGAGA
ATTATTAAAAGTAAGAAGCACCCGAAATCCTGATCTGATTTTAGACTGGCAGGATGATTTGTTTACAGGTGATTTTGATG
AAAACGGACATCAATTACGTCCGCATATTGTTTGGTTTGGAGAAATGGTTCCTGCTTTAGAAGAAGCCATCCCAATTGTA
GAAAATGCTGATTATTTTGCCGTTATTGGAACTTCGCTTCAGGTTTATCCAGCTGCAGGTTTAATTTCGTACACATACAG
TATTACTCCGGTTTTTTATATTGACCCCAAACCTATTTCTATCCCAAATATTCAAAACAAAGTTGAAGTTATCGCTAAAA
CTGCTACAGAAGGTGTTGCAGAAATGAGAACAAAATTGTTTGAGTTAGAAAATCTGTCATGA

Upstream 100 bases:

>100_bases
GAATTACGGTTTATGATGTCTGAAATTATTAAAAAAAGAACGCTGTAAAGATGTCTTACATTTTCGAAATTGTTATTTTT
ATCTTTTAATAAATTTCAAA

Downstream 100 bases:

>100_bases
ATGTAGAATCTCTATTACAGCAATTAGGTCAGTTACATTTCCTGGCTCAGTTTCTTATTTTTTCTCTCGAAAATATAAGT
TTGTTATTGATTGCTGTTTT

Product: silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKKKLVVLTGAGISAESGIKTFRDSDGLWEGHDVMEVATPEGWQKNQDLVLDFYNKRRQQLKEVQPNLGHQILAELEKDF
DVYIITQNVDDLHERAGSTKVLHLHGELLKVRSTRNPDLILDWQDDLFTGDFDENGHQLRPHIVWFGEMVPALEEAIPIV
ENADYFAVIGTSLQVYPAAGLISYTYSITPVFYIDPKPISIPNIQNKVEVIAKTATEGVAEMRTKLFELENLS

Sequences:

>Translated_233_residues
MKKKLVVLTGAGISAESGIKTFRDSDGLWEGHDVMEVATPEGWQKNQDLVLDFYNKRRQQLKEVQPNLGHQILAELEKDF
DVYIITQNVDDLHERAGSTKVLHLHGELLKVRSTRNPDLILDWQDDLFTGDFDENGHQLRPHIVWFGEMVPALEEAIPIV
ENADYFAVIGTSLQVYPAAGLISYTYSITPVFYIDPKPISIPNIQNKVEVIAKTATEGVAEMRTKLFELENLS
>Mature_233_residues
MKKKLVVLTGAGISAESGIKTFRDSDGLWEGHDVMEVATPEGWQKNQDLVLDFYNKRRQQLKEVQPNLGHQILAELEKDF
DVYIITQNVDDLHERAGSTKVLHLHGELLKVRSTRNPDLILDWQDDLFTGDFDENGHQLRPHIVWFGEMVPALEEAIPIV
ENADYFAVIGTSLQVYPAAGLISYTYSITPVFYIDPKPISIPNIQNKVEVIAKTATEGVAEMRTKLFELENLS

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=190, Percent_Identity=39.4736842105263, Blast_Score=136, Evalue=1e-32,
Organism=Homo sapiens, GI6912664, Length=208, Percent_Identity=36.0576923076923, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI13787215, Length=208, Percent_Identity=36.0576923076923, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI7657575, Length=237, Percent_Identity=27.8481012658228, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI308199517, Length=188, Percent_Identity=51.5957446808511, Blast_Score=186, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26346; Mature: 26346

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLVVLTGAGISAESGIKTFRDSDGLWEGHDVMEVATPEGWQKNQDLVLDFYNKRRQQ
CCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCCEEEECCCCCCCCCCCCEEHHHHHHHHH
LKEVQPNLGHQILAELEKDFDVYIITQNVDDLHERAGSTKVLHLHGELLKVRSTRNPDLI
HHHHCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHCCCEEEEEECCEEEEEECCCCCCEE
LDWQDDLFTGDFDENGHQLRPHIVWFGEMVPALEEAIPIVENADYFAVIGTSLQVYPAAG
EECCCCCEECCCCCCCCEECCEEEEHHHHHHHHHHHCCHHCCCCEEEEECCCEEEEECCC
LISYTYSITPVFYIDPKPISIPNIQNKVEVIAKTATEGVAEMRTKLFELENLS
EEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKKLVVLTGAGISAESGIKTFRDSDGLWEGHDVMEVATPEGWQKNQDLVLDFYNKRRQQ
CCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCCEEEECCCCCCCCCCCCEEHHHHHHHHH
LKEVQPNLGHQILAELEKDFDVYIITQNVDDLHERAGSTKVLHLHGELLKVRSTRNPDLI
HHHHCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHCCCEEEEEECCEEEEEECCCCCCEE
LDWQDDLFTGDFDENGHQLRPHIVWFGEMVPALEEAIPIVENADYFAVIGTSLQVYPAAG
EECCCCCEECCCCCCCCEECCEEEEHHHHHHHHHHHCCHHCCCCEEEEECCCEEEEECCC
LISYTYSITPVFYIDPKPISIPNIQNKVEVIAKTATEGVAEMRTKLFELENLS
EEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]