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The map label for this gene is manA [H]

Identifier: 146302684

GI number: 146302684

Start: 5990573

End: 5991802

Strand: Direct

Name: manA [H]

Synonym: Fjoh_4957

Alternate gene names: 146302684

Gene position: 5990573-5991802 (Clockwise)

Preceding gene: 146302683

Following gene: 146302685

Centisome position: 98.26

GC content: 35.85

Gene sequence:

>1230_bases
ATGAAGACTGCATTTCTTAAAACAGCATTTATAAGCCTCACTGTTCTAGCATTTATAAGTTGTTCTTCTGATAAAGAAGC
AGATCCTGTCATCATAATCGATCCGGTGGTAGAAGCAGATCCTTTAACAACTCAAAATACCGCAACATTTATGGTCGATG
CAAATGCGACAAAAGAAACCGTGGCTTTATTTTATAATTTAAAAAGACTTGCCAAAACTAAAACGGCAATTGGCCAGCAA
GATGCATTTAACAGCTTTTACCAGGATGTCGGCGGAGATTCTGACATCAAAAAAAACACAGGCTACGATCCGGCAGTTTT
AGGTTCAGATTTCATGTTTATTACAGACAAAAACAATAATAGCCAAGCAGATAACTGGTATTATCAGCAGGAACAAAAAA
TTGTAAGCGATGTGAAAGCAGCTTATGCAAAAGGAATAATCAACACGTTTTGCTGGCATTTGAGAGAGCCTAACAAAGAA
GAATCTTTTTATGCCGCCGATATGACTTCCGAAGAAAAAACAACCGCTTTTAAAAGCATTTTACCTGGTGGAGCAAACAA
TGAATGGTACAAAAAGAAATTAGATAAAATAGCCAGCGTTATTTTAAATCTAAAAGGTTCTAATGGTGAATTGATTCCCG
TAATTTTCAGACCCTTTCATGAATTTGATGGAAGCTGGTTTTGGTGGGGTGCCAGTTTTTGCACGCCCGAAGAATACAAG
CAGGCTTTTCAGTTTACTGTTGATTATTTAAAAAACACAAAAGGCGTACAGAATATTCTCTATGCTTTTTCTCCTGATAA
TTCATACACAACCGAAGTCAATTATTTAAGCCGTTATCCGGGCGATAAATATGTCGATATTATAGGAATGGATAATTATG
GAGATTTCAATAACCAAGGTCAAACAGGATCTGACAAAGCAAATGCAAAACTGAAAATTCTTTCTGATTACGCTAAAGCA
AAAGTAAAAATCGCAGCTTTAACCGAAACAGGTTATCGCGTTACCAGTAGAACGCCGGCCATTACAGATTGGTTTTCGAC
TTTATTATACAACGCTTTAACCAAAAATGATATTCAGATAAGTTATGTCATGTTTTGGAACAATAACAAAGATGGCTATT
ACGTACCAACTGGCTCAGTTTCTAACGCAGCAGATTTTAAAACGTATAGTTCTAAAACAAAATCAGCATTATTAAACACA
CTGCCTAAAATGTATGAAATGCCGAAATAA

Upstream 100 bases:

>100_bases
ATTCACTCATCCAAAAAGACAAAGCCGGATTCTGGAAATGTCCGTATCATAACAGCCGTGCCTGTTTAGAGCTTATTAAC
CGAATTAAAACCTAAAAAAA

Downstream 100 bases:

>100_bases
TATTGTACCGCAATGGCACACAGATCAAACGGATGCAGCAGGTTTTTCATTGTTGACAACTTAATGATAGATTTTAAAAG
CTTATAAATAACAATCTGTT

Product: Mannan endo-1,4-beta-mannosidase

Products: NA

Alternate protein names: Mannanase A [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKTAFLKTAFISLTVLAFISCSSDKEADPVIIIDPVVEADPLTTQNTATFMVDANATKETVALFYNLKRLAKTKTAIGQQ
DAFNSFYQDVGGDSDIKKNTGYDPAVLGSDFMFITDKNNNSQADNWYYQQEQKIVSDVKAAYAKGIINTFCWHLREPNKE
ESFYAADMTSEEKTTAFKSILPGGANNEWYKKKLDKIASVILNLKGSNGELIPVIFRPFHEFDGSWFWWGASFCTPEEYK
QAFQFTVDYLKNTKGVQNILYAFSPDNSYTTEVNYLSRYPGDKYVDIIGMDNYGDFNNQGQTGSDKANAKLKILSDYAKA
KVKIAALTETGYRVTSRTPAITDWFSTLLYNALTKNDIQISYVMFWNNNKDGYYVPTGSVSNAADFKTYSSKTKSALLNT
LPKMYEMPK

Sequences:

>Translated_409_residues
MKTAFLKTAFISLTVLAFISCSSDKEADPVIIIDPVVEADPLTTQNTATFMVDANATKETVALFYNLKRLAKTKTAIGQQ
DAFNSFYQDVGGDSDIKKNTGYDPAVLGSDFMFITDKNNNSQADNWYYQQEQKIVSDVKAAYAKGIINTFCWHLREPNKE
ESFYAADMTSEEKTTAFKSILPGGANNEWYKKKLDKIASVILNLKGSNGELIPVIFRPFHEFDGSWFWWGASFCTPEEYK
QAFQFTVDYLKNTKGVQNILYAFSPDNSYTTEVNYLSRYPGDKYVDIIGMDNYGDFNNQGQTGSDKANAKLKILSDYAKA
KVKIAALTETGYRVTSRTPAITDWFSTLLYNALTKNDIQISYVMFWNNNKDGYYVPTGSVSNAADFKTYSSKTKSALLNT
LPKMYEMPK
>Mature_409_residues
MKTAFLKTAFISLTVLAFISCSSDKEADPVIIIDPVVEADPLTTQNTATFMVDANATKETVALFYNLKRLAKTKTAIGQQ
DAFNSFYQDVGGDSDIKKNTGYDPAVLGSDFMFITDKNNNSQADNWYYQQEQKIVSDVKAAYAKGIINTFCWHLREPNKE
ESFYAADMTSEEKTTAFKSILPGGANNEWYKKKLDKIASVILNLKGSNGELIPVIFRPFHEFDGSWFWWGASFCTPEEYK
QAFQFTVDYLKNTKGVQNILYAFSPDNSYTTEVNYLSRYPGDKYVDIIGMDNYGDFNNQGQTGSDKANAKLKILSDYAKA
KVKIAALTETGYRVTSRTPAITDWFSTLLYNALTKNDIQISYVMFWNNNKDGYYVPTGSVSNAADFKTYSSKTKSALLNT
LPKMYEMPK

Specific function: Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the

COG id: COG4124

COG function: function code G; Beta-mannanase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 26 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022790
- InterPro:   IPR000805
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02156 Glyco_hydro_26 [H]

EC number: =3.2.1.78 [H]

Molecular weight: Translated: 46063; Mature: 46063

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAFLKTAFISLTVLAFISCSSDKEADPVIIIDPVVEADPLTTQNTATFMVDANATKET
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCHHH
VALFYNLKRLAKTKTAIGQQDAFNSFYQDVGGDSDIKKNTGYDPAVLGSDFMFITDKNNN
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEEEEECCCC
SQADNWYYQQEQKIVSDVKAAYAKGIINTFCWHLREPNKEESFYAADMTSEEKTTAFKSI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHH
LPGGANNEWYKKKLDKIASVILNLKGSNGELIPVIFRPFHEFDGSWFWWGASFCTPEEYK
CCCCCCCHHHHHHHHHHHHHHEEEECCCCCEEEEEECCHHHCCCCEEEECCCCCCHHHHH
QAFQFTVDYLKNTKGVQNILYAFSPDNSYTTEVNYLSRYPGDKYVDIIGMDNYGDFNNQG
HHHHHHHHHHHCCCCHHHEEEEECCCCCCEEEEHHHHHCCCCCEEEEEECCCCCCCCCCC
QTGSDKANAKLKILSDYAKAKVKIAALTETGYRVTSRTPAITDWFSTLLYNALTKNDIQI
CCCCCCCCCEEEEEEHHHHCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCEEE
SYVMFWNNNKDGYYVPTGSVSNAADFKTYSSKTKSALLNTLPKMYEMPK
EEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTAFLKTAFISLTVLAFISCSSDKEADPVIIIDPVVEADPLTTQNTATFMVDANATKET
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCHHH
VALFYNLKRLAKTKTAIGQQDAFNSFYQDVGGDSDIKKNTGYDPAVLGSDFMFITDKNNN
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEEEEECCCC
SQADNWYYQQEQKIVSDVKAAYAKGIINTFCWHLREPNKEESFYAADMTSEEKTTAFKSI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHH
LPGGANNEWYKKKLDKIASVILNLKGSNGELIPVIFRPFHEFDGSWFWWGASFCTPEEYK
CCCCCCCHHHHHHHHHHHHHHEEEECCCCCEEEEEECCHHHCCCCEEEECCCCCCHHHHH
QAFQFTVDYLKNTKGVQNILYAFSPDNSYTTEVNYLSRYPGDKYVDIIGMDNYGDFNNQG
HHHHHHHHHHHCCCCHHHEEEEECCCCCCEEEEHHHHHCCCCCEEEEEECCCCCCCCCCC
QTGSDKANAKLKILSDYAKAKVKIAALTETGYRVTSRTPAITDWFSTLLYNALTKNDIQI
CCCCCCCCCEEEEEEHHHHCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCEEE
SYVMFWNNNKDGYYVPTGSVSNAADFKTYSSKTKSALLNTLPKMYEMPK
EEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7848261 [H]