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The map label for this gene is agaA [H]

Identifier: 146302674

GI number: 146302674

Start: 5975713

End: 5976948

Strand: Reverse

Name: agaA [H]

Synonym: Fjoh_4947

Alternate gene names: 146302674

Gene position: 5976948-5975713 (Counterclockwise)

Preceding gene: 146302675

Following gene: 146302673

Centisome position: 98.03

GC content: 37.22

Gene sequence:

>1236_bases
ATGAAAAAAACAATAGTACTATTAGCTGTTTTGAGCTCATTTAATTTTGGCTTCAGCCAAGGAAATACGCACACACAAAC
AGGAGGTAAATTTGAAGGTCTTGCCATGACACCGCCAATGGGATGGAATTCATGGAATACTTTTGCAGCTAATATAGATG
AGAAACTGGTAAAAGAAACAGCAGATATTATGGTTTCGTCTGGCTTAGCTGCTGCAGGCTATAATTATATAGTTTTAGAT
GACGGCTGGATGACAAAGGAACGTGATGCAAATGGCGATCTGGTTCCTGATCCGGCTAAATTTCCTAACGGAATGAAAGC
TCTTATAGATTATGTACACAGCAAAGGTTTAAAATTTGGGTTGTATAATTGCGCAGGTACGCAGACTTGTGCAGGATATC
CCGGGACTCGCGGTTACGAATATCAGGATGCTCGTTTTTATGCCAAACTTGGAATTGATTTTTTAAAATACGATTGGTGT
AATACAAAAGGCATAACAGCGCCAGAAGCTTATACCACAATGAGTAATGCTTTAAAAACTGCTGGAAGACCAATTGTTTT
CAGTCTTTGCGAATGGGGTGATAATCAGCCGTGGGAGTGGGGTAAACCAGTTGGAAATCTTTGGAGAATATCCGGCGATA
TTTATCCTTGTTTTGACTGCGAATTCAAACATCCTGAAAATTGGTCTTCGTGGGGATTCATGAAAATTGCCGAAATGCGA
AAAGATATCCGTAAATATTCCGGCCCGGATCATTGGAATGATTTTGATATGATGGAAGTAGGCAACGAAATGAACGATAC
CGAAGATAAATCGCATTTTGCTATGTGGTGTATGCTTTCTTCACCATTGTTTACCGGAAATGACTATAGAAAAATGTCTA
AAGAAACCTTGGCAATTTTAACAAACAAGGAGTTAATCGCGATAAATCAGGATAAATTAGGAATACAAGGATTTAAACAT
TCTGCTGAAGACGGTTTAGAAGTTTGGGTAAAACCTTTGTCTGATGGAAATTGGGCAGTAACTTTTTTAAATAGAAGTGA
TGTTTCTAAAAAGATTAATTTCGACTGGAAAAAGAATACTTTTAAAGATGTTGATTTTGGTTACGAAGCTGATTTTAATA
AAACAGCATTCAAGCTAAAAAATCTTTGGACAAATAAAGAAGCAGGAAGCACCAAAAAGAACTTTACGGCAGAGTTAGCT
TCTCATGACTGCATAACATTGAAACTATCTCTTTAA

Upstream 100 bases:

>100_bases
AGAAAGGACTCCAATTCGAGACATCGATTTGGAGTTTTTTATATATTATTTAAATATTTCTATAAGCCCATAATAAACAA
AATATCAAAAATAAAGTAAA

Downstream 100 bases:

>100_bases
AAATAATGATATGATTAAGTTAAAAAAAAATTTAATTACAGCGGCTCTTTTATTAATCTGTTGTTTCTCGCAGGCACAGT
TTGTAAAAGAACATGGCCAG

Product: alpha-galactosidase

Products: NA

Alternate protein names: Alpha-D-galactoside galactohydrolase; Alpha-galactosidase 27A; Melibiase [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MKKTIVLLAVLSSFNFGFSQGNTHTQTGGKFEGLAMTPPMGWNSWNTFAANIDEKLVKETADIMVSSGLAAAGYNYIVLD
DGWMTKERDANGDLVPDPAKFPNGMKALIDYVHSKGLKFGLYNCAGTQTCAGYPGTRGYEYQDARFYAKLGIDFLKYDWC
NTKGITAPEAYTTMSNALKTAGRPIVFSLCEWGDNQPWEWGKPVGNLWRISGDIYPCFDCEFKHPENWSSWGFMKIAEMR
KDIRKYSGPDHWNDFDMMEVGNEMNDTEDKSHFAMWCMLSSPLFTGNDYRKMSKETLAILTNKELIAINQDKLGIQGFKH
SAEDGLEVWVKPLSDGNWAVTFLNRSDVSKKINFDWKKNTFKDVDFGYEADFNKTAFKLKNLWTNKEAGSTKKNFTAELA
SHDCITLKLSL

Sequences:

>Translated_411_residues
MKKTIVLLAVLSSFNFGFSQGNTHTQTGGKFEGLAMTPPMGWNSWNTFAANIDEKLVKETADIMVSSGLAAAGYNYIVLD
DGWMTKERDANGDLVPDPAKFPNGMKALIDYVHSKGLKFGLYNCAGTQTCAGYPGTRGYEYQDARFYAKLGIDFLKYDWC
NTKGITAPEAYTTMSNALKTAGRPIVFSLCEWGDNQPWEWGKPVGNLWRISGDIYPCFDCEFKHPENWSSWGFMKIAEMR
KDIRKYSGPDHWNDFDMMEVGNEMNDTEDKSHFAMWCMLSSPLFTGNDYRKMSKETLAILTNKELIAINQDKLGIQGFKH
SAEDGLEVWVKPLSDGNWAVTFLNRSDVSKKINFDWKKNTFKDVDFGYEADFNKTAFKLKNLWTNKEAGSTKKNFTAELA
SHDCITLKLSL
>Mature_411_residues
MKKTIVLLAVLSSFNFGFSQGNTHTQTGGKFEGLAMTPPMGWNSWNTFAANIDEKLVKETADIMVSSGLAAAGYNYIVLD
DGWMTKERDANGDLVPDPAKFPNGMKALIDYVHSKGLKFGLYNCAGTQTCAGYPGTRGYEYQDARFYAKLGIDFLKYDWC
NTKGITAPEAYTTMSNALKTAGRPIVFSLCEWGDNQPWEWGKPVGNLWRISGDIYPCFDCEFKHPENWSSWGFMKIAEMR
KDIRKYSGPDHWNDFDMMEVGNEMNDTEDKSHFAMWCMLSSPLFTGNDYRKMSKETLAILTNKELIAINQDKLGIQGFKH
SAEDGLEVWVKPLSDGNWAVTFLNRSDVSKKINFDWKKNTFKDVDFGYEADFNKTAFKLKNLWTNKEAGSTKKNFTAELA
SHDCITLKLSL

Specific function: Hydrolyzes galactomannan found in plant cell wall, by cleaving alpha-1,6-D-galactose side-chains from the mannan backbone. Appears to act in synergy with mannanase (ManA) to elicit hydrolysis of galactomannan. Has greater activity against galactomannans w

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 27 family [H]

Homologues:

Organism=Homo sapiens, GI4504009, Length=389, Percent_Identity=37.2750642673522, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI4557781, Length=347, Percent_Identity=37.463976945245, Blast_Score=210, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17562946, Length=338, Percent_Identity=36.6863905325444, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24583253, Length=387, Percent_Identity=36.1757105943152, Blast_Score=224, Evalue=8e-59,
Organism=Drosophila melanogaster, GI19922382, Length=344, Percent_Identity=37.2093023255814, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24654142, Length=344, Percent_Identity=37.2093023255814, Blast_Score=220, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013780
- InterPro:   IPR002241
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 46480; Mature: 46480

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00512 ALPHA_GALACTOSIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTIVLLAVLSSFNFGFSQGNTHTQTGGKFEGLAMTPPMGWNSWNTFAANIDEKLVKET
CCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEECCHHHHHHHHH
ADIMVSSGLAAAGYNYIVLDDGWMTKERDANGDLVPDPAKFPNGMKALIDYVHSKGLKFG
HHHHHHCCCHHCCCCEEEECCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCEEE
LYNCAGTQTCAGYPGTRGYEYQDARFYAKLGIDFLKYDWCNTKGITAPEAYTTMSNALKT
EEECCCCCCCCCCCCCCCCCCCHHEEEEHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
AGRPIVFSLCEWGDNQPWEWGKPVGNLWRISGDIYPCFDCEFKHPENWSSWGFMKIAEMR
CCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHH
KDIRKYSGPDHWNDFDMMEVGNEMNDTEDKSHFAMWCMLSSPLFTGNDYRKMSKETLAIL
HHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHEEEEE
TNKELIAINQDKLGIQGFKHSAEDGLEVWVKPLSDGNWAVTFLNRSDVSKKINFDWKKNT
ECCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCC
FKDVDFGYEADFNKTAFKLKNLWTNKEAGSTKKNFTAELASHDCITLKLSL
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MKKTIVLLAVLSSFNFGFSQGNTHTQTGGKFEGLAMTPPMGWNSWNTFAANIDEKLVKET
CCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEECCHHHHHHHHH
ADIMVSSGLAAAGYNYIVLDDGWMTKERDANGDLVPDPAKFPNGMKALIDYVHSKGLKFG
HHHHHHCCCHHCCCCEEEECCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCEEE
LYNCAGTQTCAGYPGTRGYEYQDARFYAKLGIDFLKYDWCNTKGITAPEAYTTMSNALKT
EEECCCCCCCCCCCCCCCCCCCHHEEEEHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
AGRPIVFSLCEWGDNQPWEWGKPVGNLWRISGDIYPCFDCEFKHPENWSSWGFMKIAEMR
CCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHH
KDIRKYSGPDHWNDFDMMEVGNEMNDTEDKSHFAMWCMLSSPLFTGNDYRKMSKETLAIL
HHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHEEEEE
TNKELIAINQDKLGIQGFKHSAEDGLEVWVKPLSDGNWAVTFLNRSDVSKKINFDWKKNT
ECCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCC
FKDVDFGYEADFNKTAFKLKNLWTNKEAGSTKKNFTAELASHDCITLKLSL
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA