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The map label for this gene is trpA

Identifier: 146302626

GI number: 146302626

Start: 5920021

End: 5920782

Strand: Direct

Name: trpA

Synonym: Fjoh_4899

Alternate gene names: 146302626

Gene position: 5920021-5920782 (Clockwise)

Preceding gene: 146302625

Following gene: 146302627

Centisome position: 97.1

GC content: 33.86

Gene sequence:

>762_bases
ATGAACAGAATAACTCAAAAATTACAAGAAGATAAAAAGATCCTTTCGATCTATTTTTCTGCGGGATATCCAAATTTAAA
TGATACCGTGCAGATTATTCAGGATTTAGAAAAAAACGGAGTTGATTTAATCGAAATCGGACTTCCTTTCAGTGATCCTT
TGGCAGACGGACCAACTATTCAGGCGAGTTCTACACAGGCACTTCATAACGGAATGACAACTCAAATTCTTTTTGATCAG
CTGCAAAACATCCGCGAAAGCGTAAAAATTCCATTAATCATTATGGGATATTTCAACCCGATGTTACAATACGGAGTTGA
AGCTTTCTGCAAAAAATGTGCTGAAATTGGTATTGATGGTCTAATTATCCCAGATCTTCCGGTTGATGTTTATGCAGATG
AATACAAAGCAATTTTCGAAAAATACGGTTTAATTAATGTATTCCTAATTACACCTCAAACTTCAGATGAACGTATTCGT
TTTATAGACAGCGTTTCAAACGGATTTATTTATATGGTAAGTTCTGCAAGTGTTACAGGATCACAAAGTGGTTTTGGAAA
TGTTCAGGAAACCTATTTTGAAAGAATTTCAAATCTAAATTTAAAAAATCCTCAAATTGTTGGTTTTGGAATTTCAAATA
AAGAAACTTTTAACCAAGCCACTAAATATGCAAAAGGAGCTATTATTGGAAGTGCTTTTATCAAACATTTAAGCGAAAGC
GGCAGTGGGAAAATTCAAGAGTTTGTTGGAGAGATTCGATAA

Upstream 100 bases:

>100_bases
AATCAGCTTAAATCAATTTAAATCTGCGTGAAATATTTTTCAAATAAACTTAATATACCTACAAGGTTTTGAAAGCCTTG
CAGTAGAAAAACATATCAAA

Downstream 100 bases:

>100_bases
TTAAATGTAAATTACACGGTATTTCAATTTTAGCAGCCGTGTTATGTTCCAATATTCCATTAAAAAAATAGTCTTTCTAT
TTTTCTTAAATTTTATATTT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNRITQKLQEDKKILSIYFSAGYPNLNDTVQIIQDLEKNGVDLIEIGLPFSDPLADGPTIQASSTQALHNGMTTQILFDQ
LQNIRESVKIPLIIMGYFNPMLQYGVEAFCKKCAEIGIDGLIIPDLPVDVYADEYKAIFEKYGLINVFLITPQTSDERIR
FIDSVSNGFIYMVSSASVTGSQSGFGNVQETYFERISNLNLKNPQIVGFGISNKETFNQATKYAKGAIIGSAFIKHLSES
GSGKIQEFVGEIR

Sequences:

>Translated_253_residues
MNRITQKLQEDKKILSIYFSAGYPNLNDTVQIIQDLEKNGVDLIEIGLPFSDPLADGPTIQASSTQALHNGMTTQILFDQ
LQNIRESVKIPLIIMGYFNPMLQYGVEAFCKKCAEIGIDGLIIPDLPVDVYADEYKAIFEKYGLINVFLITPQTSDERIR
FIDSVSNGFIYMVSSASVTGSQSGFGNVQETYFERISNLNLKNPQIVGFGISNKETFNQATKYAKGAIIGSAFIKHLSES
GSGKIQEFVGEIR
>Mature_253_residues
MNRITQKLQEDKKILSIYFSAGYPNLNDTVQIIQDLEKNGVDLIEIGLPFSDPLADGPTIQASSTQALHNGMTTQILFDQ
LQNIRESVKIPLIIMGYFNPMLQYGVEAFCKKCAEIGIDGLIIPDLPVDVYADEYKAIFEKYGLINVFLITPQTSDERIR
FIDSVSNGFIYMVSSASVTGSQSGFGNVQETYFERISNLNLKNPQIVGFGISNKETFNQATKYAKGAIIGSAFIKHLSES
GSGKIQEFVGEIR

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=208, Percent_Identity=32.2115384615385, Blast_Score=117, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6321412, Length=237, Percent_Identity=38.3966244725738, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_FLAJ1 (A5FA76)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001197217.1
- ProteinModelPortal:   A5FA76
- SMR:   A5FA76
- STRING:   A5FA76
- GeneID:   5094169
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_4899
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   TSDERIR
- ProtClustDB:   PRK13111
- BioCyc:   FJOH376686:FJOH_4899-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28043; Mature: 28043

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 45-45 ACT_SITE 56-56

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRITQKLQEDKKILSIYFSAGYPNLNDTVQIIQDLEKNGVDLIEIGLPFSDPLADGPTI
CCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEE
QASSTQALHNGMTTQILFDQLQNIRESVKIPLIIMGYFNPMLQYGVEAFCKKCAEIGIDG
CCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
LIIPDLPVDVYADEYKAIFEKYGLINVFLITPQTSDERIRFIDSVSNGFIYMVSSASVTG
EECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCC
SQSGFGNVQETYFERISNLNLKNPQIVGFGISNKETFNQATKYAKGAIIGSAFIKHLSES
CCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSGKIQEFVGEIR
CCCHHHHHHHCCC
>Mature Secondary Structure
MNRITQKLQEDKKILSIYFSAGYPNLNDTVQIIQDLEKNGVDLIEIGLPFSDPLADGPTI
CCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEE
QASSTQALHNGMTTQILFDQLQNIRESVKIPLIIMGYFNPMLQYGVEAFCKKCAEIGIDG
CCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
LIIPDLPVDVYADEYKAIFEKYGLINVFLITPQTSDERIRFIDSVSNGFIYMVSSASVTG
EECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCC
SQSGFGNVQETYFERISNLNLKNPQIVGFGISNKETFNQATKYAKGAIIGSAFIKHLSES
CCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSGKIQEFVGEIR
CCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA