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The map label for this gene is oprM [H]

Identifier: 146302588

GI number: 146302588

Start: 5881471

End: 5882910

Strand: Reverse

Name: oprM [H]

Synonym: Fjoh_4861

Alternate gene names: 146302588

Gene position: 5882910-5881471 (Counterclockwise)

Preceding gene: 146302589

Following gene: 146302586

Centisome position: 96.49

GC content: 35.56

Gene sequence:

>1440_bases
ATGTATAAAGTTAAAACATATCAATACAGTATTGCATTGGGCTTATGTCTTGCCGTGGCAGGATGTAAAGCTCCTGCAGC
TGAAACAGCAAGTACAAGTGCTCCTGTTCCTGAAGCATTTGGAAACGTTAAATCTCAGGATACAACTAATACTGCAGCCT
TAAAATGGAAAGATTTCTTTAAGGATCAAAATTTAGTTGAACTAATCGATATTGCGCTGAAAAACAATCAGGAACTGAAT
ATGACTTTGCAGGAAATTGAAATTGCAAAGAATGATATCAGAGTTCGTAAAGGCGCTTTACTGCCAACAGTTGGTGTTCG
TGCCGGTGCAGGAATCGAAAAAGTGGGTAGATATACAAGTCAGGGAGCAGGTGATGCTACAACAGAAATAAAACCTGGAG
TAGAAACACCAGATCCATTACCGGATTATACCATTGCTGCTTATGCAAATTGGGAAGTAGATATCTGGAAAAAACTGCGT
AATTCTAAAAAGGCAGCTGTAAACCGTTATTTAGCTACAGTTGAAGGTAAAAACTTTGTAATTACAAACTTAATTGCTGA
AATCGCTGATTCTTATTATGAATTATTAGCTTTAGACAGCCAATTGGAAATCGTAAAACAGACAATTGAATTACAGAGCA
ATGCTTTGGAAATTGTAAAAGTTCAGAAACAAGCAGCTAGAGCTACAGAACTTGGAGTTCAGAAGTTTCAGGCTGAGGTT
TTAACTTCAAAAAGTTTAGAGTTTGATATTCTTCAGAAAATTAAAGAAAACGAAAACAAAATCAACTTTTTGTTAGGACG
TTATCCACAGGAAATAAAAAGAGAGAAAACCAATTTTACAGACTTGCTTCCGGCAGTTGTAAACTCTGGAATTCCATCTC
AGTTACTTGCAAATCGTCCAGATGTTAAACAGGCCGAATTAGAGTTAGTAGCTGCAAAACTAGATGTAAAAGTAGCAAAG
GCTGAATTTTATCCTTCATTGGATATTTCTGCTGCTTTTGGAGTTCAGGCTTTTAAACCTTCTTATTTGTTTACATTCCC
TGAGTCAATTTTGTATTCATTAGCAGGAGATCTTGCTGCGCCGCTTATTAATAGAAATGCTATTAAAGCAGAGTTTTCAA
GCGCTAATGCAAGACAGCTTCAGGCTTTGTACAATTACGATAAAACAATTTTGAATGCATATTTAGAAGTTTCAACACAA
CTTTCTAAAATCGAAAATCTTCAAAAAGGTTACGATTTAAAATCACAGCAGGTTGATGCTTTAAATAGATCAATTGATGT
TTCAAATGATTTGTTTAAATCTGCGAGAGTAGATTATTTTGAAGTTTTGATGACGCAGAGAGATGCATTAGAAGCAAAAC
TTGAATTAATCGACACTAAAAAAGAACAATTAAATGCTGCGGTTCATGTTTACAGAGACCTGGGCGGCGGATGGAAATAA

Upstream 100 bases:

>100_bases
ATTCCGGGCTTGTATTTCATTTTTGCCAAAATTGCTGAGAAACACAAACTGGTAAAACATGAAGAAGAAAATCCATTAAC
AGAAGAAATTGATAACAATC

Downstream 100 bases:

>100_bases
TTTTGCCAATTAATTTGTAGAAAAAAAGCCTTTCGGAAGTGAAGTTCTGAAAGGCTTTTTTGTTTTATGAATTTAAAGAA
TGATCAAACCTGTATTTTGA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MYKVKTYQYSIALGLCLAVAGCKAPAAETASTSAPVPEAFGNVKSQDTTNTAALKWKDFFKDQNLVELIDIALKNNQELN
MTLQEIEIAKNDIRVRKGALLPTVGVRAGAGIEKVGRYTSQGAGDATTEIKPGVETPDPLPDYTIAAYANWEVDIWKKLR
NSKKAAVNRYLATVEGKNFVITNLIAEIADSYYELLALDSQLEIVKQTIELQSNALEIVKVQKQAARATELGVQKFQAEV
LTSKSLEFDILQKIKENENKINFLLGRYPQEIKREKTNFTDLLPAVVNSGIPSQLLANRPDVKQAELELVAAKLDVKVAK
AEFYPSLDISAAFGVQAFKPSYLFTFPESILYSLAGDLAAPLINRNAIKAEFSSANARQLQALYNYDKTILNAYLEVSTQ
LSKIENLQKGYDLKSQQVDALNRSIDVSNDLFKSARVDYFEVLMTQRDALEAKLELIDTKKEQLNAAVHVYRDLGGGWK

Sequences:

>Translated_479_residues
MYKVKTYQYSIALGLCLAVAGCKAPAAETASTSAPVPEAFGNVKSQDTTNTAALKWKDFFKDQNLVELIDIALKNNQELN
MTLQEIEIAKNDIRVRKGALLPTVGVRAGAGIEKVGRYTSQGAGDATTEIKPGVETPDPLPDYTIAAYANWEVDIWKKLR
NSKKAAVNRYLATVEGKNFVITNLIAEIADSYYELLALDSQLEIVKQTIELQSNALEIVKVQKQAARATELGVQKFQAEV
LTSKSLEFDILQKIKENENKINFLLGRYPQEIKREKTNFTDLLPAVVNSGIPSQLLANRPDVKQAELELVAAKLDVKVAK
AEFYPSLDISAAFGVQAFKPSYLFTFPESILYSLAGDLAAPLINRNAIKAEFSSANARQLQALYNYDKTILNAYLEVSTQ
LSKIENLQKGYDLKSQQVDALNRSIDVSNDLFKSARVDYFEVLMTQRDALEAKLELIDTKKEQLNAAVHVYRDLGGGWK
>Mature_479_residues
MYKVKTYQYSIALGLCLAVAGCKAPAAETASTSAPVPEAFGNVKSQDTTNTAALKWKDFFKDQNLVELIDIALKNNQELN
MTLQEIEIAKNDIRVRKGALLPTVGVRAGAGIEKVGRYTSQGAGDATTEIKPGVETPDPLPDYTIAAYANWEVDIWKKLR
NSKKAAVNRYLATVEGKNFVITNLIAEIADSYYELLALDSQLEIVKQTIELQSNALEIVKVQKQAARATELGVQKFQAEV
LTSKSLEFDILQKIKENENKINFLLGRYPQEIKREKTNFTDLLPAVVNSGIPSQLLANRPDVKQAELELVAAKLDVKVAK
AEFYPSLDISAAFGVQAFKPSYLFTFPESILYSLAGDLAAPLINRNAIKAEFSSANARQLQALYNYDKTILNAYLEVSTQ
LSKIENLQKGYDLKSQQVDALNRSIDVSNDLFKSARVDYFEVLMTQRDALEAKLELIDTKKEQLNAAVHVYRDLGGGWK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=475, Percent_Identity=24.8421052631579, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI2367348, Length=344, Percent_Identity=21.8023255813953, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53118; Mature: 53118

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKVKTYQYSIALGLCLAVAGCKAPAAETASTSAPVPEAFGNVKSQDTTNTAALKWKDFF
CCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHEEEHHHHH
KDQNLVELIDIALKNNQELNMTLQEIEIAKNDIRVRKGALLPTVGVRAGAGIEKVGRYTS
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHC
QGAGDATTEIKPGVETPDPLPDYTIAAYANWEVDIWKKLRNSKKAAVNRYLATVEGKNFV
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHHHHHHEECCCCCH
ITNLIAEIADSYYELLALDSQLEIVKQTIELQSNALEIVKVQKQAARATELGVQKFQAEV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTSKSLEFDILQKIKENENKINFLLGRYPQEIKREKTNFTDLLPAVVNSGIPSQLLANRP
HHCCCCCHHHHHHHHCCCCCEEHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCHHHHCCCC
DVKQAELELVAAKLDVKVAKAEFYPSLDISAAFGVQAFKPSYLFTFPESILYSLAGDLAA
CCHHHHHHHHHHHHHHEEEHHHCCCCCCCHHHCCCHHCCCCEEEECHHHHHHHHHHHHHH
PLINRNAIKAEFSSANARQLQALYNYDKTILNAYLEVSTQLSKIENLQKGYDLKSQQVDA
HHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LNRSIDVSNDLFKSARVDYFEVLMTQRDALEAKLELIDTKKEQLNAAVHVYRDLGGGWK
HHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYKVKTYQYSIALGLCLAVAGCKAPAAETASTSAPVPEAFGNVKSQDTTNTAALKWKDFF
CCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHEEEHHHHH
KDQNLVELIDIALKNNQELNMTLQEIEIAKNDIRVRKGALLPTVGVRAGAGIEKVGRYTS
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHC
QGAGDATTEIKPGVETPDPLPDYTIAAYANWEVDIWKKLRNSKKAAVNRYLATVEGKNFV
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHHHHHHEECCCCCH
ITNLIAEIADSYYELLALDSQLEIVKQTIELQSNALEIVKVQKQAARATELGVQKFQAEV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTSKSLEFDILQKIKENENKINFLLGRYPQEIKREKTNFTDLLPAVVNSGIPSQLLANRP
HHCCCCCHHHHHHHHCCCCCEEHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCHHHHCCCC
DVKQAELELVAAKLDVKVAKAEFYPSLDISAAFGVQAFKPSYLFTFPESILYSLAGDLAA
CCHHHHHHHHHHHHHHEEEHHHCCCCCCCHHHCCCHHCCCCEEEECHHHHHHHHHHHHHH
PLINRNAIKAEFSSANARQLQALYNYDKTILNAYLEVSTQLSKIENLQKGYDLKSQQVDA
HHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LNRSIDVSNDLFKSARVDYFEVLMTQRDALEAKLELIDTKKEQLNAAVHVYRDLGGGWK
HHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]