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The map label for this gene is truA [H]

Identifier: 146302394

GI number: 146302394

Start: 5643692

End: 5644546

Strand: Direct

Name: truA [H]

Synonym: Fjoh_4667

Alternate gene names: 146302394

Gene position: 5643692-5644546 (Clockwise)

Preceding gene: 146302392

Following gene: 146302395

Centisome position: 92.57

GC content: 35.09

Gene sequence:

>855_bases
ATGAGACGTTTTTACAACATCCCTAAAAAGAAAACTTTGAACCTCTGTCCCTTTGAACCTTTGAACCTCAAAAAGAAAAA
ATCTTTGTACCTTTGCACCTCAAAACCTCTGTACCTCACAATGAGATATTTTATTCATTTTGCTTATAACGGAACTCATT
ATCATGGCTGGCAGTTTCAGCCAAATGCTTCTTCAGTTCAGGAAACTTTAAATAAAGCGCTTTCGGTTTTATTAAATTCA
ACCATAAATGTTATGGGCGCCGGAAGAACAGATACTGGGGTGCATGCCGAGGAAATGTACGGACATTTTGATTTAGAAAA
ATCTATTGATATTCCGGTTTTGGTTCATAAACTCAATTCGTATTTACCAAAAGATATTGCGGTTTACAACCTGATTCCGG
TACATGATGATGCGCATTGTAGATTTGACGCAACAAAAAGAACATACGAATATCATATCAATACAGTTAAAAATCCGTTT
TTAGAAGAATTGAGCTGGTACGTGACTCAAAAGCTCGATGTTGAGTTAATGAATGAGGCTGCAAAGATATTATTGAAGCA
TACCGATTTTCAGTGTTTCTCAAAAGTAAATACAGATGTTAATACTTTTGACTGCACGATTTTTGAGGCCTATTGGAAAC
AGGAAAATAACAAACTGGTTTTTACAATTTCGGCAAACCGTTTTTTACGAAACATGGTTCGGGCAATTGTGGGAACTTTA
ATTAATATAGGATTACACAAAATCACGCTGGCAGATTTTGAAAATATTATTGCCAGCAAAAACAGAGAAAAAGCCGGATT
TTCGGTTCCGGCGCATGGTTTGTATTTAACCAAAATTTATTACGATTATTTATAA

Upstream 100 bases:

>100_bases
GGTACAAAGGTTTTTCCTGTATAAAAACATTAATAACTATGTGTAAATTCTGTCTTTATTTACTTATTAGACATTATGTG
CCTGCTTTGAATCTCTACAA

Downstream 100 bases:

>100_bases
GCTTTAAGCTTTAGGCAATAGGCTTCAGGCTTATTTCTTACTGTTTACTGCTAATAGCTTACAGCCTAAAGCCTAAAGCT
TAAAGCATCAAAAAAATGAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MRRFYNIPKKKTLNLCPFEPLNLKKKKSLYLCTSKPLYLTMRYFIHFAYNGTHYHGWQFQPNASSVQETLNKALSVLLNS
TINVMGAGRTDTGVHAEEMYGHFDLEKSIDIPVLVHKLNSYLPKDIAVYNLIPVHDDAHCRFDATKRTYEYHINTVKNPF
LEELSWYVTQKLDVELMNEAAKILLKHTDFQCFSKVNTDVNTFDCTIFEAYWKQENNKLVFTISANRFLRNMVRAIVGTL
INIGLHKITLADFENIIASKNREKAGFSVPAHGLYLTKIYYDYL

Sequences:

>Translated_284_residues
MRRFYNIPKKKTLNLCPFEPLNLKKKKSLYLCTSKPLYLTMRYFIHFAYNGTHYHGWQFQPNASSVQETLNKALSVLLNS
TINVMGAGRTDTGVHAEEMYGHFDLEKSIDIPVLVHKLNSYLPKDIAVYNLIPVHDDAHCRFDATKRTYEYHINTVKNPF
LEELSWYVTQKLDVELMNEAAKILLKHTDFQCFSKVNTDVNTFDCTIFEAYWKQENNKLVFTISANRFLRNMVRAIVGTL
INIGLHKITLADFENIIASKNREKAGFSVPAHGLYLTKIYYDYL
>Mature_284_residues
MRRFYNIPKKKTLNLCPFEPLNLKKKKSLYLCTSKPLYLTMRYFIHFAYNGTHYHGWQFQPNASSVQETLNKALSVLLNS
TINVMGAGRTDTGVHAEEMYGHFDLEKSIDIPVLVHKLNSYLPKDIAVYNLIPVHDDAHCRFDATKRTYEYHINTVKNPF
LEELSWYVTQKLDVELMNEAAKILLKHTDFQCFSKVNTDVNTFDCTIFEAYWKQENNKLVFTISANRFLRNMVRAIVGTL
INIGLHKITLADFENIIASKNREKAGFSVPAHGLYLTKIYYDYL

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=29.7101449275362, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1788657, Length=235, Percent_Identity=36.5957446808511, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 33009; Mature: 33009

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFYNIPKKKTLNLCPFEPLNLKKKKSLYLCTSKPLYLTMRYFIHFAYNGTHYHGWQFQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHEEEEHEEEEECCCEECCEEEC
PNASSVQETLNKALSVLLNSTINVMGAGRTDTGVHAEEMYGHFDLEKSIDIPVLVHKLNS
CCHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
YLPKDIAVYNLIPVHDDAHCRFDATKRTYEYHINTVKNPFLEELSWYVTQKLDVELMNEA
HCCCCEEEEEEEECCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
AKILLKHTDFQCFSKVNTDVNTFDCTIFEAYWKQENNKLVFTISANRFLRNMVRAIVGTL
HHHHHHCCCHHHHHHCCCCCCEEEEHEEEHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHH
INIGLHKITLADFENIIASKNREKAGFSVPAHGLYLTKIYYDYL
HHCCHHEEEHHHHHHHHHCCCCCCCCCCCCCCCEEEEHEEHHCC
>Mature Secondary Structure
MRRFYNIPKKKTLNLCPFEPLNLKKKKSLYLCTSKPLYLTMRYFIHFAYNGTHYHGWQFQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHEEEEHEEEEECCCEECCEEEC
PNASSVQETLNKALSVLLNSTINVMGAGRTDTGVHAEEMYGHFDLEKSIDIPVLVHKLNS
CCHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
YLPKDIAVYNLIPVHDDAHCRFDATKRTYEYHINTVKNPFLEELSWYVTQKLDVELMNEA
HCCCCEEEEEEEECCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
AKILLKHTDFQCFSKVNTDVNTFDCTIFEAYWKQENNKLVFTISANRFLRNMVRAIVGTL
HHHHHHCCCHHHHHHCCCCCCEEEEHEEEHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHH
INIGLHKITLADFENIIASKNREKAGFSVPAHGLYLTKIYYDYL
HHCCHHEEEHHHHHHHHHCCCCCCCCCCCCCCCEEEEHEEHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA