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The map label for this gene is nasB [H]

Identifier: 146302351

GI number: 146302351

Start: 5588108

End: 5590621

Strand: Direct

Name: nasB [H]

Synonym: Fjoh_4624

Alternate gene names: 146302351

Gene position: 5588108-5590621 (Clockwise)

Preceding gene: 146302349

Following gene: 146302352

Centisome position: 91.66

GC content: 38.62

Gene sequence:

>2514_bases
ATGATTAAAGTAATAGTAGTTGGAAACGGCATGGTTGGTTATAAATTTTGCGAAAAATTCGTTGCAAAATCAGGACAAGA
AAAGTATCAAATCACAGTATTTGGAGAAGAACCAAGACGCGCTTACGATCGCGTTCACTTAAGCGAGTACTTTGGAGGAA
AAACAGCTGATGACTTATCATTATCAACAAGCCAATGGTACGCAGAAAACAATATTATTCTAAATACATCTGAACTAATT
ACAGATATAAATCGTGATGCAAAAACAATACATACACACTTAGGCAAAACACATACGTACGACTACTTAGTTTTAGCAAC
AGGTTCTTCCGCTTTTGTACCTCCAATTGAAGGTGTAGAAAAAGAAGGTGTTTTTGTCTATAGAACCATCGAAGATCTGG
ATGCTATCATGTCCTATGCAAAAAAAATAAAAATAAAAGGCGCAACCGAAGCTGCAGTTTTAGGTGGCGGTTTATTAGGA
TTAGAAGCAGCAAAAGCAGTTCGTGATTTAGGCTTAAATCCGCATGTGGTGGAATTTGCGCCGCGTTTAATGCCGAGACA
ACTAGATCAGGGCGCAAGTGATATGCTTCAATCAAAAATTGAAGAATTAAACATCGGAATTCATCTTAACAAAGCAACCC
AATATATCGACGGAAAAGAAAGTATAACAGGAATGATGTTTGCAAACGACGAATTGTTAAAAGTAGACATGTTGGTTATT
TCTGCCGGAATCAAACCTCGCGACGAACTGGCAAGAGTTTCAGGGCTTGAAGTTGGCGTACGAGGCGGAATTGTGGTAAA
CAATCAAATGCGAACATCAGATCCTTTTATTTTTGCGATTGGCGAAGCAGCTCTTTACAACCAAAACATTTACGGACTTG
TTGCTCCTGGTTACGAAATGGCTGATGTCGCTGCTGAGCAGATCTTAAATGGCGACAAAACCATGAGAGAAACCATCGAT
ATGTCGACTCAATTGAAATTAATTGGTGTTGAAGTAGCGAGTTTTGGTGATCCTTTTATTGAAAACAATGACGTTACCGC
TATTATTTATGAAAATAAATTAAGCAGCGTTTACAAAAGAATCAACGTTACAAAAGATTCTAAAAAACTTTTAGGCGGAA
TTTTAGTTGGAGATTCCAGCGATTACAATTCCCTTTTCCAGATTTATATCAATGAAATGGCATTGCCTAAAAATCCTGAA
GATTTGATTCTGGGTTCAAGAGACGGTTCAGAAGGTTCTCCTTTAGGAAGCGTGATGGATTTGCCGGATACAGCCGTAAT
CTGTTCTTGCGAGAATGTTACCAAAGGTGCGATCTGCTGTAAAATTTTAGACGAAAGCTGTTCTTCTCTTTCAGATGTTG
TAAAAGCAACCAAAGCAACTTCAGGATGCGGCGGCTGTAAACCAATGGTTTCTGATTTAGTAAAAGCGACTCAAAAATCT
TTAGGAAAAGAAGTAAAAGAAGTGATCTGTGAGCATTTCAATTATAATCGTCAGGAACTATTTGATTTGGTAAAAATCAA
TAAATACGAGAATTTCTACGATGTTCTGGATCATCACGGAAAAGGCGATGGTTGCGAAATCTGTAAACCAGTTGTCGCTT
CTATTTTCTCTAGTATCTATAACGACACTGCAAACAAACACGTGACAACGCAAGATACAAACGATAGATTTTTAGCTAAT
ATTCAACGAAACGGAACGTATTCTGTAGTTCCGAGAGTTGCCGGAGGCGAAATTACTGCCGAAAAACTAATCGTAATAGG
TGAAGTTGCTAAACAATTCGATTTGTACACTAAAATTACGGGAGCTCAAAGAGTGGATTTATTCGGAGCACATTTGAGTG
ATCTTCCAAAAATCTGGAAAATTTTAATTGACAACGGTTTTGAAAGTGGTCATGCTTACGGAAAATCACTTCGTGCCGTA
AAAAGCTGTGTGGGAAATGCTTGGTGCCGTTACGGAATGGACGACAGCGCCGGATTTGCCATCGAACTAGAAAATAGATA
TAAAGGAATCCGTTCTCCGCATAAACTCAAAGGTGGTGTTTCTGCCTGCATTCGCGAATGCGCTGAAGCACGCGGAAAAG
ATTTCGGATTAATTGCCGTTGAAGGCGGTTGGAATTTATACATCGCAGGAAATGGCGGTGCAAATCCAAAACACGCGGTT
TTACTAGCAGAGAAAATCGACAAAGAAACCGTAATCAAATACATGGATCGTTTCTTAATGTATTACATCCGAACCGCTGG
CCCGCTGATTAGAACTTCAACTTGGTTAGAAAAACTAGATGGAGGCTTAGAATATTTAAAACAAGTAATTATCGAAGACA
GTTTAGGAATCTGCGAAACGCTTGAAGCTGAAATGCAGACTTTGGTAAATACTTTCGAATGCGAATGGAAACAAGTGCTG
GAAAAACCAAGATTACTAAAACGATTCAATCACTTTATAAACTCAGACGAGAAAGACGATAATGCAGTTTTTGTTCCGTT
ACGAGATCAAAAAATGCCTAAAGCTTGGTCGTAA

Upstream 100 bases:

>100_bases
AGTAATAAAAACCTCCATCTACTTAAAAAAATAAAATTATTACGTATTTTAATAAGTATAAATACTTATATTTGCGTAGT
AATACTTAATTAAAGAAATA

Downstream 100 bases:

>100_bases
CTTAAAAACATAATTTTTTAAACACATAGAGACATAGATTTTAAAAACTTAATAAAGGCGTTTCACTTGCATAAATACAC
ATAGCTATGTGTTAGAAACT

Product: nitrite reductase (NAD(P)H), large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 837; Mature: 837

Protein sequence:

>837_residues
MIKVIVVGNGMVGYKFCEKFVAKSGQEKYQITVFGEEPRRAYDRVHLSEYFGGKTADDLSLSTSQWYAENNIILNTSELI
TDINRDAKTIHTHLGKTHTYDYLVLATGSSAFVPPIEGVEKEGVFVYRTIEDLDAIMSYAKKIKIKGATEAAVLGGGLLG
LEAAKAVRDLGLNPHVVEFAPRLMPRQLDQGASDMLQSKIEELNIGIHLNKATQYIDGKESITGMMFANDELLKVDMLVI
SAGIKPRDELARVSGLEVGVRGGIVVNNQMRTSDPFIFAIGEAALYNQNIYGLVAPGYEMADVAAEQILNGDKTMRETID
MSTQLKLIGVEVASFGDPFIENNDVTAIIYENKLSSVYKRINVTKDSKKLLGGILVGDSSDYNSLFQIYINEMALPKNPE
DLILGSRDGSEGSPLGSVMDLPDTAVICSCENVTKGAICCKILDESCSSLSDVVKATKATSGCGGCKPMVSDLVKATQKS
LGKEVKEVICEHFNYNRQELFDLVKINKYENFYDVLDHHGKGDGCEICKPVVASIFSSIYNDTANKHVTTQDTNDRFLAN
IQRNGTYSVVPRVAGGEITAEKLIVIGEVAKQFDLYTKITGAQRVDLFGAHLSDLPKIWKILIDNGFESGHAYGKSLRAV
KSCVGNAWCRYGMDDSAGFAIELENRYKGIRSPHKLKGGVSACIRECAEARGKDFGLIAVEGGWNLYIAGNGGANPKHAV
LLAEKIDKETVIKYMDRFLMYYIRTAGPLIRTSTWLEKLDGGLEYLKQVIIEDSLGICETLEAEMQTLVNTFECEWKQVL
EKPRLLKRFNHFINSDEKDDNAVFVPLRDQKMPKAWS

Sequences:

>Translated_837_residues
MIKVIVVGNGMVGYKFCEKFVAKSGQEKYQITVFGEEPRRAYDRVHLSEYFGGKTADDLSLSTSQWYAENNIILNTSELI
TDINRDAKTIHTHLGKTHTYDYLVLATGSSAFVPPIEGVEKEGVFVYRTIEDLDAIMSYAKKIKIKGATEAAVLGGGLLG
LEAAKAVRDLGLNPHVVEFAPRLMPRQLDQGASDMLQSKIEELNIGIHLNKATQYIDGKESITGMMFANDELLKVDMLVI
SAGIKPRDELARVSGLEVGVRGGIVVNNQMRTSDPFIFAIGEAALYNQNIYGLVAPGYEMADVAAEQILNGDKTMRETID
MSTQLKLIGVEVASFGDPFIENNDVTAIIYENKLSSVYKRINVTKDSKKLLGGILVGDSSDYNSLFQIYINEMALPKNPE
DLILGSRDGSEGSPLGSVMDLPDTAVICSCENVTKGAICCKILDESCSSLSDVVKATKATSGCGGCKPMVSDLVKATQKS
LGKEVKEVICEHFNYNRQELFDLVKINKYENFYDVLDHHGKGDGCEICKPVVASIFSSIYNDTANKHVTTQDTNDRFLAN
IQRNGTYSVVPRVAGGEITAEKLIVIGEVAKQFDLYTKITGAQRVDLFGAHLSDLPKIWKILIDNGFESGHAYGKSLRAV
KSCVGNAWCRYGMDDSAGFAIELENRYKGIRSPHKLKGGVSACIRECAEARGKDFGLIAVEGGWNLYIAGNGGANPKHAV
LLAEKIDKETVIKYMDRFLMYYIRTAGPLIRTSTWLEKLDGGLEYLKQVIIEDSLGICETLEAEMQTLVNTFECEWKQVL
EKPRLLKRFNHFINSDEKDDNAVFVPLRDQKMPKAWS
>Mature_837_residues
MIKVIVVGNGMVGYKFCEKFVAKSGQEKYQITVFGEEPRRAYDRVHLSEYFGGKTADDLSLSTSQWYAENNIILNTSELI
TDINRDAKTIHTHLGKTHTYDYLVLATGSSAFVPPIEGVEKEGVFVYRTIEDLDAIMSYAKKIKIKGATEAAVLGGGLLG
LEAAKAVRDLGLNPHVVEFAPRLMPRQLDQGASDMLQSKIEELNIGIHLNKATQYIDGKESITGMMFANDELLKVDMLVI
SAGIKPRDELARVSGLEVGVRGGIVVNNQMRTSDPFIFAIGEAALYNQNIYGLVAPGYEMADVAAEQILNGDKTMRETID
MSTQLKLIGVEVASFGDPFIENNDVTAIIYENKLSSVYKRINVTKDSKKLLGGILVGDSSDYNSLFQIYINEMALPKNPE
DLILGSRDGSEGSPLGSVMDLPDTAVICSCENVTKGAICCKILDESCSSLSDVVKATKATSGCGGCKPMVSDLVKATQKS
LGKEVKEVICEHFNYNRQELFDLVKINKYENFYDVLDHHGKGDGCEICKPVVASIFSSIYNDTANKHVTTQDTNDRFLAN
IQRNGTYSVVPRVAGGEITAEKLIVIGEVAKQFDLYTKITGAQRVDLFGAHLSDLPKIWKILIDNGFESGHAYGKSLRAV
KSCVGNAWCRYGMDDSAGFAIELENRYKGIRSPHKLKGGVSACIRECAEARGKDFGLIAVEGGWNLYIAGNGGANPKHAV
LLAEKIDKETVIKYMDRFLMYYIRTAGPLIRTSTWLEKLDGGLEYLKQVIIEDSLGICETLEAEMQTLVNTFECEWKQVL
EKPRLLKRFNHFINSDEKDDNAVFVPLRDQKMPKAWS

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=344, Percent_Identity=24.4186046511628, Blast_Score=88, Evalue=4e-17,
Organism=Homo sapiens, GI226437568, Length=344, Percent_Identity=24.4186046511628, Blast_Score=88, Evalue=4e-17,
Organism=Homo sapiens, GI65787454, Length=344, Percent_Identity=24.4186046511628, Blast_Score=88, Evalue=4e-17,
Organism=Escherichia coli, GI1789765, Length=836, Percent_Identity=50.9569377990431, Blast_Score=843, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=320, Percent_Identity=25.625, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788892, Length=259, Percent_Identity=25.4826254826255, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI87082354, Length=243, Percent_Identity=26.3374485596708, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1787352, Length=268, Percent_Identity=27.2388059701493, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=282, Percent_Identity=23.4042553191489, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281359715, Length=256, Percent_Identity=25.78125, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281359713, Length=256, Percent_Identity=25.78125, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24639250, Length=256, Percent_Identity=25.78125, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18543267, Length=256, Percent_Identity=25.78125, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24639252, Length=256, Percent_Identity=25.78125, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24639257, Length=295, Percent_Identity=25.7627118644068, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585130, Length=282, Percent_Identity=22.3404255319149, Blast_Score=68, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327
- InterPro:   IPR017941 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 92344; Mature: 92344

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00022 EGF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVIVVGNGMVGYKFCEKFVAKSGQEKYQITVFGEEPRRAYDRVHLSEYFGGKTADDLS
CEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCC
LSTSQWYAENNIILNTSELITDINRDAKTIHTHLGKTHTYDYLVLATGSSAFVPPIEGVE
CCCHHEEECCCEEEEHHHHHHHHCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCC
KEGVFVYRTIEDLDAIMSYAKKIKIKGATEAAVLGGGLLGLEAAKAVRDLGLNPHVVEFA
CCCEEEEEEHHHHHHHHHHHHHEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHH
PRLMPRQLDQGASDMLQSKIEELNIGIHLNKATQYIDGKESITGMMFANDELLKVDMLVI
HHHHHHHHCCCHHHHHHHHHHHHCCCEEECCHHHHCCCHHHEEEEEEECCCEEEEEEEEE
SAGIKPRDELARVSGLEVGVRGGIVVNNQMRTSDPFIFAIGEAALYNQNIYGLVAPGYEM
ECCCCCHHHHHHHCCCEEECCCCEEECCCCCCCCCEEEEECCHHHCCCCEEEEECCCCHH
ADVAAEQILNGDKTMRETIDMSTQLKLIGVEVASFGDPFIENNDVTAIIYENKLSSVYKR
HHHHHHHHHCCCHHHHHHHCCCCEEEEEEEEHHHCCCCCCCCCCEEEEEECCHHHHHHHH
INVTKDSKKLLGGILVGDSSDYNSLFQIYINEMALPKNPEDLILGSRDGSEGSPLGSVMD
HCCCCCHHHHHCEEEECCCCCHHHHHHHHHHHHCCCCCCHHCEEECCCCCCCCCCCCHHC
LPDTAVICSCENVTKGAICCKILDESCSSLSDVVKATKATSGCGGCKPMVSDLVKATQKS
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LGKEVKEVICEHFNYNRQELFDLVKINKYENFYDVLDHHGKGDGCEICKPVVASIFSSIY
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
NDTANKHVTTQDTNDRFLANIQRNGTYSVVPRVAGGEITAEKLIVIGEVAKQFDLYTKIT
CCCCCCEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
GAQRVDLFGAHLSDLPKIWKILIDNGFESGHAYGKSLRAVKSCVGNAWCRYGMDDSAGFA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
IELENRYKGIRSPHKLKGGVSACIRECAEARGKDFGLIAVEGGWNLYIAGNGGANPKHAV
EEEHHHHCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEEECCCCCCCCEE
LLAEKIDKETVIKYMDRFLMYYIRTAGPLIRTSTWLEKLDGGLEYLKQVIIEDSLGICET
EEHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LEAEMQTLVNTFECEWKQVLEKPRLLKRFNHFINSDEKDDNAVFVPLRDQKMPKAWS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MIKVIVVGNGMVGYKFCEKFVAKSGQEKYQITVFGEEPRRAYDRVHLSEYFGGKTADDLS
CEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCC
LSTSQWYAENNIILNTSELITDINRDAKTIHTHLGKTHTYDYLVLATGSSAFVPPIEGVE
CCCHHEEECCCEEEEHHHHHHHHCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCC
KEGVFVYRTIEDLDAIMSYAKKIKIKGATEAAVLGGGLLGLEAAKAVRDLGLNPHVVEFA
CCCEEEEEEHHHHHHHHHHHHHEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHH
PRLMPRQLDQGASDMLQSKIEELNIGIHLNKATQYIDGKESITGMMFANDELLKVDMLVI
HHHHHHHHCCCHHHHHHHHHHHHCCCEEECCHHHHCCCHHHEEEEEEECCCEEEEEEEEE
SAGIKPRDELARVSGLEVGVRGGIVVNNQMRTSDPFIFAIGEAALYNQNIYGLVAPGYEM
ECCCCCHHHHHHHCCCEEECCCCEEECCCCCCCCCEEEEECCHHHCCCCEEEEECCCCHH
ADVAAEQILNGDKTMRETIDMSTQLKLIGVEVASFGDPFIENNDVTAIIYENKLSSVYKR
HHHHHHHHHCCCHHHHHHHCCCCEEEEEEEEHHHCCCCCCCCCCEEEEEECCHHHHHHHH
INVTKDSKKLLGGILVGDSSDYNSLFQIYINEMALPKNPEDLILGSRDGSEGSPLGSVMD
HCCCCCHHHHHCEEEECCCCCHHHHHHHHHHHHCCCCCCHHCEEECCCCCCCCCCCCHHC
LPDTAVICSCENVTKGAICCKILDESCSSLSDVVKATKATSGCGGCKPMVSDLVKATQKS
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LGKEVKEVICEHFNYNRQELFDLVKINKYENFYDVLDHHGKGDGCEICKPVVASIFSSIY
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
NDTANKHVTTQDTNDRFLANIQRNGTYSVVPRVAGGEITAEKLIVIGEVAKQFDLYTKIT
CCCCCCEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
GAQRVDLFGAHLSDLPKIWKILIDNGFESGHAYGKSLRAVKSCVGNAWCRYGMDDSAGFA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
IELENRYKGIRSPHKLKGGVSACIRECAEARGKDFGLIAVEGGWNLYIAGNGGANPKHAV
EEEHHHHCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEEECCCCCCCCEE
LLAEKIDKETVIKYMDRFLMYYIRTAGPLIRTSTWLEKLDGGLEYLKQVIIEDSLGICET
EEHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LEAEMQTLVNTFECEWKQVLEKPRLLKRFNHFINSDEKDDNAVFVPLRDQKMPKAWS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]