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The map label for this gene is amiB [H]

Identifier: 146302312

GI number: 146302312

Start: 5544103

End: 5545224

Strand: Reverse

Name: amiB [H]

Synonym: Fjoh_4585

Alternate gene names: 146302312

Gene position: 5545224-5544103 (Counterclockwise)

Preceding gene: 146302315

Following gene: 146302311

Centisome position: 90.95

GC content: 34.49

Gene sequence:

>1122_bases
ATGAATGGTTTAAACAAAATTAAGGTAATCTTTACTTTTTTTCTAACGATATTGTCATTTTGCTCTTATAGTCAATCAAA
TGTTTTTAAAGTAACACTTGATGCCGGACACGGAGATCATGACTTTGGTGCTGTTTACAGCGGCCGGATTGAAAAAAATA
TTGCCTTAGCAATTGTTTTAAAAGTGGGGAAAATTTTAGAGCTTAATCCAAATGTTAATGTAATTTATACTCGTAAAACA
GATGTTTTCGTGGATTTGGTCGAAAGAGCTAATATTGCCAACAGAGCAAATTCAAATATTTTTGTTTCTATTCACTGTAA
TGCTAACAAAAATACAGCTGCAGACGGAACCGAAACTTATGTAATGGGTTTGAGTAAAGTTGCCTCGAATCTTGAAGCAG
CAAAAAAAGAGAACTCTGTAATTACTTTAGAAAAAGATTATAAACGAAAATATGAAGGTTTCGACCCAAATTCGCCAGAA
TCTATGATCGGAATGACTTTGATGCAGGAAGAATATTTAGACAATAGTATTTCTCTGGCAACTAAAATCGAAGATAATTT
TGAAAAATTAGGTAAAAAACTTCGTCAGGGAGGTGTAAAACAAGCACCTTTCATGGTACTTCACAAAGCATACATGCCAA
GAGTTTTAGTAGAAACGGGATTTGTCTCTAATCCAACAGAAGGTAATATTTTAAATTCTGAAGAAGGTCAGGATGATATA
GCAAAAGCTATTGCAGAAGCAATTCTGAGCTATAAAAGAGAGTACTTTGGTTCAGGGGGCGAGTCAGACGATAGTCGGCC
CATAAGAGATACTACTCCCGCAAAACCAAAAACAACTGCTCCGGCTGCAGTAACTAAAAATGCACCAAAAGGAACGTTTT
TCAAAGTACAGCTAATCGCAAGTATTAAAAAAACACCATTGGAGCCAAAAAACTTTAAAGGACTAAAAAATGTAACTATG
TTATTTGAAAACAATATATATAAGTACTTCTATCAGGAAACTTCAGATTATGAAACTGCAAAGAAATATCTGGAAGAGGC
TAAAAGTAAAGGGTATGGCGCTTCCTTTTTAGTTGCAACCAAAGACGGAGAAAAGATTAGTATTCAGGACGCGATCAAAT
AA

Upstream 100 bases:

>100_bases
TGTTTTTTGACATGTCAAACTTACATATAATTTTTTGGCAAAAATAATCAATTGTAAAAATTATAACAGCTGCATTTTAT
TAAAATTAACTTTTAGATTT

Downstream 100 bases:

>100_bases
TCGTCACAAGTTCGAATATTTATATTAATTTTGTAACAAACACTAAGAATTTTGAAAGTAACAAGAGAAATTAAAACGGC
TATTTTAGTCATCGCATCAA

Product: cell wall hydrolase/autolysin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MNGLNKIKVIFTFFLTILSFCSYSQSNVFKVTLDAGHGDHDFGAVYSGRIEKNIALAIVLKVGKILELNPNVNVIYTRKT
DVFVDLVERANIANRANSNIFVSIHCNANKNTAADGTETYVMGLSKVASNLEAAKKENSVITLEKDYKRKYEGFDPNSPE
SMIGMTLMQEEYLDNSISLATKIEDNFEKLGKKLRQGGVKQAPFMVLHKAYMPRVLVETGFVSNPTEGNILNSEEGQDDI
AKAIAEAILSYKREYFGSGGESDDSRPIRDTTPAKPKTTAPAAVTKNAPKGTFFKVQLIASIKKTPLEPKNFKGLKNVTM
LFENNIYKYFYQETSDYETAKKYLEEAKSKGYGASFLVATKDGEKISIQDAIK

Sequences:

>Translated_373_residues
MNGLNKIKVIFTFFLTILSFCSYSQSNVFKVTLDAGHGDHDFGAVYSGRIEKNIALAIVLKVGKILELNPNVNVIYTRKT
DVFVDLVERANIANRANSNIFVSIHCNANKNTAADGTETYVMGLSKVASNLEAAKKENSVITLEKDYKRKYEGFDPNSPE
SMIGMTLMQEEYLDNSISLATKIEDNFEKLGKKLRQGGVKQAPFMVLHKAYMPRVLVETGFVSNPTEGNILNSEEGQDDI
AKAIAEAILSYKREYFGSGGESDDSRPIRDTTPAKPKTTAPAAVTKNAPKGTFFKVQLIASIKKTPLEPKNFKGLKNVTM
LFENNIYKYFYQETSDYETAKKYLEEAKSKGYGASFLVATKDGEKISIQDAIK
>Mature_373_residues
MNGLNKIKVIFTFFLTILSFCSYSQSNVFKVTLDAGHGDHDFGAVYSGRIEKNIALAIVLKVGKILELNPNVNVIYTRKT
DVFVDLVERANIANRANSNIFVSIHCNANKNTAADGTETYVMGLSKVASNLEAAKKENSVITLEKDYKRKYEGFDPNSPE
SMIGMTLMQEEYLDNSISLATKIEDNFEKLGKKLRQGGVKQAPFMVLHKAYMPRVLVETGFVSNPTEGNILNSEEGQDDI
AKAIAEAILSYKREYFGSGGESDDSRPIRDTTPAKPKTTAPAAVTKNAPKGTFFKVQLIASIKKTPLEPKNFKGLKNVTM
LFENNIYKYFYQETSDYETAKKYLEEAKSKGYGASFLVATKDGEKISIQDAIK

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=231, Percent_Identity=30.7359307359307, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788776, Length=224, Percent_Identity=31.6964285714286, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87082163, Length=244, Percent_Identity=29.9180327868852, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 41398; Mature: 41398

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLNKIKVIFTFFLTILSFCSYSQSNVFKVTLDAGHGDHDFGAVYSGRIEKNIALAIVL
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEECCCCCCCEEEEEEE
KVGKILELNPNVNVIYTRKTDVFVDLVERANIANRANSNIFVSIHCNANKNTAADGTETY
ECCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHH
VMGLSKVASNLEAAKKENSVITLEKDYKRKYEGFDPNSPESMIGMTLMQEEYLDNSISLA
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEE
TKIEDNFEKLGKKLRQGGVKQAPFMVLHKAYMPRVLVETGFVSNPTEGNILNSEEGQDDI
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHH
AKAIAEAILSYKREYFGSGGESDDSRPIRDTTPAKPKTTAPAAVTKNAPKGTFFKVQLIA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEEEEH
SIKKTPLEPKNFKGLKNVTMLFENNIYKYFYQETSDYETAKKYLEEAKSKGYGASFLVAT
HHHHCCCCCCCCCCHHHHHHHHHCCEEEEEHHCCCCHHHHHHHHHHHHCCCCCCEEEEEE
KDGEKISIQDAIK
CCCCEEEHHCCCC
>Mature Secondary Structure
MNGLNKIKVIFTFFLTILSFCSYSQSNVFKVTLDAGHGDHDFGAVYSGRIEKNIALAIVL
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEECCCCCCCEEEEEEE
KVGKILELNPNVNVIYTRKTDVFVDLVERANIANRANSNIFVSIHCNANKNTAADGTETY
ECCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHH
VMGLSKVASNLEAAKKENSVITLEKDYKRKYEGFDPNSPESMIGMTLMQEEYLDNSISLA
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEE
TKIEDNFEKLGKKLRQGGVKQAPFMVLHKAYMPRVLVETGFVSNPTEGNILNSEEGQDDI
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHH
AKAIAEAILSYKREYFGSGGESDDSRPIRDTTPAKPKTTAPAAVTKNAPKGTFFKVQLIA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEEEEH
SIKKTPLEPKNFKGLKNVTMLFENNIYKYFYQETSDYETAKKYLEEAKSKGYGASFLVAT
HHHHCCCCCCCCCCHHHHHHHHHCCEEEEEHHCCCCHHHHHHHHHHHHCCCCCCEEEEEE
KDGEKISIQDAIK
CCCCEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511774; 7610040; 9278503; 1594459 [H]